Objective To investigate the effects of losing ratio of orthodontic brackets bonding after prolonged etching time (prolonged etching time method) and microabrasion with normal acid etching time (etching after microabrasion method) to different degree of dental fluorosis.Methods A total of 30 patients with dental fluorosis were selected from the cases treated in the Department of Stomatology,Lianyungang Hospital of Traditional Chinese Medicine.They were classified as 3 groups from mild to severe degree according to Dean's criteria,10 people per group.Patients within each group were randomly assigned to 2 subgroups,5 people per subgroup;prolonged etching time method and etching after microabrasion method were used,respectively,in each subgroup.The total and first losing ratios of brackets at five time points (1,2,4,12 and 24 weeks) were observed.The losing ratio differences of orthodontic brackets between the two disposing measures bonding to different degree of dental fluorosis were analyzed.Resuts The total bracket loss rates were 38.3％ (46/120) and 21.4％ (25/117),respectively,in severe dental fluorosis disposed by prolonged etching time method and etching after microabrasion method.It was significantly higher than that of the same processing method of mild and moderate dental fluorosis groups,in which the total bracket loss rates were 12.5％ (15/120),18.1％ (21/116),7.6％ (9/118) and 5.8％ (7/120),respectively,and the differences were statistically significant (x2 =21.12,11.87,8.96,12.24,all P ＜ 0.01).The total loss rate of etching after microabrasion method was lower than that of prolonged etching time method in mild dental fluorosis group,the difference was not statistically significant (x2 =1.56,P ＞ 0.05).The total loss rates of etching after microabrasion method were lower than that of prolonged etching time method in moderate and severe dental fluorosis groups,and the differences were statistically significant (x2 =8.49,8.13,all P ＜ 0.05).The medians of first bracket loss rates were 8.3％ and 4.3％,respectively,in severe dental fluorosis disposed by prolonged etching time method and etching after microabrasion method.They were significantly higher than that of the same processing method of mild and moderate dental fluorosis groups,in which the medians of first bracket loss rates were 2.5％,4.3％,1.7％ and 0.8％,respectively,and the differences were statistically significant (U =2.619,2.627,2.312,2.627,all P ＜ 0.05).The average percentage of first bracket loss rates of etching after microabrasion method was lower than that of prolonged etching time method in mild dental fluorosis group,the difference was not statistically significant (U =-0.949,P ＞ 0.05).The average percentages of first bracket loss rates of etching after microabrasion method were lower than those of prolonged etching time method in moderate and severe dental fluorosis groups,and the differences were statistically significant (U =-2.214,-2.410,all P＜ 0.05).Conclusions Whether in prolonged etching time method subgroup or etching after microabrasion method subgroup,the more serious injury of dental fluorosis is,the higher loss rate of orthodontic bracket will be.For the clinical treatment of mild and severe dental fluorosis,the effect of etching after microabrasion method is better than prolonging etching time method.

Objective To explore the value of electroencephalogram (EEG) in children with headache.Methods 180 children with headache were carried out EEG recording,and the electrical activity of the brain was recorded and analyzed.Results In 180 cases of children,EEG was normal in 56 cases,abnormalities in 124 cases,the abnormal rate was 68.89％.The mild abnormalities was 78 cases,in a proportion to 43.33％ in the total record:moderate abnormalities was 14 cases,in a proportion to 7.78％ in the total reccrd;high-grede,abnormalities was 2 cases,in a proportion to 1.11％ ;5 patients was in critical state,in a proportion to 2.78％ ;epileptiform activity was 25 cases,in a proportion to 13.89％.97 normal cases with intermittent headache,abnormal rate was 46.1 1％,which was significantly lower than the attack headache ( x2 =15.16,P ＜ 0.05 ).Conclusion Headache in children with EEG examination had important significance.

Humans ; Organ Transplantation ; legislation & jurisprudence ; Patient Selection ; Tissue Banks ; legislation & jurisprudence ; organization & administration ; Transplant Recipients

Objective To investigate the effect of Shengxue-zengbai Decoction on the expression of ICAM-1 and SCF in bone marrow tissue and on the mechanism of hematopoietic reconstitution in bone marrow transplantation (BMT). Methods The experimental animals were randomly divided into S groups: normal group received no treatment, BMT control group, G-CSF group, Shengxue group, and Shengxue +G-CSF group. BMT mouse models were established. Shengxue group, Shengxue + G-CSF group were given Shengxuezengbai Decoction. On days 9, 14, 24 after BMT, mice were killed and peripheral blood cells, bone marrow nucleated cells were detected. Histological observation of bone was made and the ICAM-1 and SCF expression were assayed by immunohistochemistry. Results In Shengxue-zengbai Decoction group, the peripheral blood cell, bone marrow nucleated cell on the days 9, 14, 24 after BMT were higher than the other control groups. The expression of ICAM-1 and SCF than in the other control groups (P<0.05 or P<0.01 > . Conclusion Shengxue-zengbai Decoction enhances ICAM-1 and SCF expression in bone marrow after syngenic bone marrow transplantation in mice, which may be related to the mechanisms of its accelerating hematopoietic reconstitution in allogenic bone marrow transplantation.

OBJECTIVETo evaluate the effect of taper and inserting angle on stability of micro-implants in type II bone and to provide theoretical evidence for clinical selection of taper and inserting angle.

METHODSThree-dimensional finite element models of type II bone sections and micro-implants were fabricated. They were designed using different tapers (diameter between 1.1 to 1.6 mm, taper between 0 to 0.0625) and different inserting angles ranging from 30 degrees to 90 degrees (30 degrees, 45 degrees, 60 degrees, 75 degrees, 90 degrees). Effects on the maximum von Mises stresses in mandible and micro-implants, as well as on the maximum displacements in micro-implants, were analyzed under an applied 2 N horizontal force.

RESULTSThe inserting angle had a significant effect on stresses and displacements, with lower distribution of stresses and displacements occurring at 60 degrees. The effect of the taper was also related to the inserting angle. The minimum stresses were 5.013 4 MPa (cortical bone) and 25.1310 MPa (micro-implant) in model D at 60 degrees. The displacements of micro-implants were proportional to the taper.

CONCLUSIONThe inserting angle has a significant effect on stability of micro-implants, and the recommended inserting angle is 60 degrees. The influence of taper on the stability of micro-implants is related to the inserting angle. The micro-implant with 0.0375 taper (maximum diameter is 1.6 mm, minimum diameter is 1.3 mm) is more suitable for type II bones.

OBJECTIVE:To establish a method for the simultaneous determination of ethambutol(EMB)and isoniazid(INH) in children’s plasma. METHODS:After precipitated with acetonitrile,plasma samples were determined by UPLC-MS/MS method. Using lamivudine as internal standard,Waters ACQUITY? UPLC HSS T3 column was adopted with mobile phase consisted of ace-tonitrile-water(containing 0.05% formic acid and 15 mmol/L ammonium formate)in gradient mode at the flow rate of 0.2 ml/min. By ESI,positive ion detection was conducted in MRM mode. The monitoring transition ion-pair was m/z 205.2→116.0 for EMB, m/z 138.1→121.1 for INH and m/z 230.2→111.9 for internal standard. RESULTS:The linear range of EMB and INH was 10-5 000 ng/ml and 50-5 000 ng/ml,respectively;the limits of quantitation were 10 and 50 ng/ml;RSDs of inter-day and intra-day were all lower than 15% and relative recoveries were 98.7%-105.5%. Plasma concentrations of EMB and INH in 4 children with tuberculo-sis were 94.5-99.7 and 55.1-80.9 ng/ml. CONCLUSIONS:The method is rapid and sensitive,which suitable for children’s plasma concentration monitoring of EMB and INH and pharmacokinetic study.

Doctor-Patient knowledge transfer plays an important role in clinical service.In an evidence-based investigation on their basic features and preference,patients investigated fall into six categories according four influence factors.These factors comprise,set as the criteria,information preference,decision-making preference,perceived severity of disease and influence of reference group.The six groups are the contradiction group,reactive group,ego group,decision preference group,total equality group,and positive group.Doctor-Patient knowledge transfer stategiea are suggested based on the preference features of the six groups.These strategies serve as references and paramedics for doctors to perform effective doctor-patient communication.

Objective To investigate the relationship between TCM syndrome of hyperlipidemia and indexes such as age,sex,BMI,TC,TG,LDL-C,HDL-C,ApoA,ApoB,the vascular media,coagulation and inflammatory indices.Methods 200 patients of hyperlipidemia recruited from the cadre ward in hospital were made TCM syndrome differentiation according to Guiding principles for the clinical study of Chinese Medicines.SPSS16.0 statistical software were adopted for analyzing the relation between TCM syndrome and indexes such as age,sex,BMI,TC,TG,LDL-C,HDL-C,ApoA,ApoB,the vascular media,coagulation and inflammatory indices.Results ①)There are no differences TCM syndrome and the indexes of age,sex,TC,TG,HDL-C,LDL-C,VLDL-C,lipoproteins,the vascular media,coagulation and inflammatory indices.②Compared with the liver kidney yin deficiency syndrome group,apolipoprotein A was higher in the groups of qi stagnation and blood stasis,phlegm stagnation syndrome,and hyperactivity of Yang due to yin deficiency syndrome,the difference was statistically significant; while APOB showed no difference among the groups.Conclusion TCM syndrome of hyperlipidemia had no relation to indexes of age,sex,body mass index,blood lipid,and blood coagulation.The study found a correlation between hyperlipidemia and phlegm and Qi deficiency,which can provide a theoretical basis for syndrome differentiation of TCM.

Objective To study material basis of Zhou's prescription, components of effective parts Ⅰ in Zhou's prescription was gualitative and quantitative analyzed. Methods The components of effective part Ⅰ was identified by test-tube reaction and qualitative TLC method, the content of saponins and flavones was quantitative analyzed. Results Total content of the saponins and flavone are 1.07% and 0.85% in Zhou's Prescription. The purity of saponins and flavones in effective parts Ⅰ are 37.4% and 29.7%. Conclusion The main components of effective part Ⅰ in Zhou's Prescription were flavones and saponins.

To raise clinical curative effect through quantitative and synthesize analysis of Traditional Chinese Medicine (TCM). According to Zhongjing’s medicine usage regulation and the author’s clinical fulfillment, a generalization, contrast and synthesize analysis among the same kind medicines is carried on in the text. It put forward the conception of "First-line medicine" for the first time and carried on detailed treatise of its nature, effect, classification, application and regulation. Meanwhile, the important role and function of "First-line medicine" is conf irmed in the text, and hope it is wished to helpful to the usage of TCM in clinical practice.