2.Perioperative nursing of patients with incomplete cervical spinal cord injury caused by traumatic cervical spine fracture
Jie PAN ; Jing CHEN ; Dan ZHAO
Modern Clinical Nursing 2016;15(1):48-51
Objective To investigate the effect and perioperative nursing experience of surgical operation on patients with incomplete cervical spinal cord injury caused by traumatic cervical spine fracture. Method Fifty-nine patients with traumatic cervical spine fracture in our hospital from January 2011 to 2014 were nursed perioperatively, including before operation , training accttenty with swallowing and blowing balloons besides basic nursing care , and after operation , mental care , close observation and functional exercises postoperatively. Results The operation time was (110.5+13.0) min, the amount of bleeding was (155.6+7.5)mL. Three patients developed hematoma compression, 13 patients developed high fever. All patients were cured after treatment and nursing. Conclusion Preoperative training of swallowing and blowing balloon is fundamental for ensured surgical effect and intraoperative surgical cooperation and close observation together with postoperative mental care, close obsersation and functional exercise are the guarantee for surgical effect.
3.Role of endoplasmic reticulum stress in high fattyacid induced injury in cardiomyocytes
Dan CHEN ; Jiliang WU ; Jing LI
Chinese Pharmacological Bulletin 2017;33(7):966-971
Aim To explore the role of endoplasmic reticulum stress (EPR) in high fatty acid induced injury in cardiomyocytes.Methods Cardiomyocytes were exposed to different concentrations of palmitic acid (0, 0.1, 0.2, 0.4 mmol·L-1) for 24 h and different time points of high palmitic acid (0.2 mmol·L-1) (12, 24, 48 h).Cell viability was measured by CCK8, and the protein expressions of EPR such as GRP78, CHOP, PERKphos, IRE1phos, ATF6 and apoptosis related proteins such as Bcl-2 and Bax were determined by Western blot.Results When the cells were stimulated with palmitic acid (0.1~0.4 mmol·L-1) for 24 h, the cell proliferation rates of 0.2 and 0.4 mmol·L-1 groups were significantly decreased.Cardiomyocytes exposured to high palmitic acid (0.2 mmol·L-1) for 24 h showed an increase in theexpression of EPR related proteins (GRP78, CHOP, PERKphos, IRE1phos and ATF6) and Bax(P<0.05), while Bcl-2 expression was significantly reduced.Pretreatmented with EPR inhibitor pravastatin(10 mol·L-1) significantly increased high palmitic acid induced Bcl-2 expression (P<0.05) and significantly decreased high palmitic acid induced Bax expression (P<0.05).Conclusion Activated EPR might play an important role in treatment of high fatty acid induced myocardial injury.
4.Personality Characteristics of 1921 Cadets in an Air Force Academy Shown by the MBTI-G
Jing CHEN ; Dan-Min MIAO ; Yang WANG ;
Chinese Mental Health Journal 2002;0(11):-
Objective:To investigate influence of living locations on the personality characteristics of the only child and none-only children in an air force academy.Methods:1921 cadets were tested by the Chinese version of the MBTI-G.Results:Scores of the only child cadets from cities on the Sensing-Intuition and Thinking-Feeling dimensions were higher than those of none-only children group from cities and those of the only child group from countries.The scores of them on the SN scale were 93.0?18.5,90.4?17.6 and 90.1?17.7(t=2.25,P=0.025;t=1.99,P= 0.048).The scores of them on the TF scale were 94.6?22.6,89.1?21.1 and 88.4?21.0(t=4.02,P
5.Clinical feature of hyperthyroidism in elderly patients and therapeutic comparison between ~(131)Ⅰand antithyroid drugs
Dan-Yun CHEN ; Jin JING ; Tang-Hua CHEN ;
Chinese Journal of Endocrinology and Metabolism 2000;0(06):-
One hundred elderly patients with hyperthyroidism were enrolled in a paired design study.The outcome of ~(131)Ⅰand antithyroid drug(ATD)treatments was examined after 2 years.The results showed that ~(131)Ⅰcould be recognized as the safer,more convenient and effective treatment than ATD for the elderly patients with hyperthyroidism.
6.Investigation on the related factors of clinical incidence and the ultrasound diagnostic value of endometrial polyps
Jing CHEN ; Weiying WU ; Jun MENG ; Dan LIAN ; Liuyu JI
Chinese Journal of Postgraduates of Medicine 2013;36(27):18-20
Objective To investigate the related factors of clinical incidence and the ultrasound diagnostic value of endometrial polyps (EMP).Methods A total of 278 patients with clinical abnormal vaginal bleeding,menorrhagia,infertility,cervical polyps or physical examination showing EMP received vaginal ultrasound and hysteroscopy.The related clinical factors were recorded and single factors and multivariate unconditional Logistic regression analysis were carried.Results The univariate analysis showed that the number of pregnancy,menopause,placing intrauterine device,oral comtraceptives,history of hormone therapy,chronic endometritis,cervical polyp,merger endometriosis and obesity were the statistically significant factors of EMP incidence (P < 0.05 or < 0.01),which were used as dependent variables for multivariate Logistic regression analysis,indicating that menopause,chronic endometritis,history of hormone therapy and cervical polyp significantly affecting EMP incidence (P < 0.05),with OR values of 4.068,4.122,3.770 and 3.983 respectively.Conclusion Menopause,chronic endometritis,history of hormone therapy,cervical polyps are the related risk factors of EMP.
7.Chitin hybrid membrane carrying cells repairs corneal epithelial injury
Jing LU ; Shike WU ; Guang CHEN ; Yue ZHAO ; Dan LI
Chinese Journal of Tissue Engineering Research 2017;21(2):232-237
BACKGROUND:Chitin has been found to be a good biomaterial, but research on chitin carrying corneal epithelial cel s for rabbit corneal epithelial injury is little reported. OBJECTIVE:To investigate the repair outcomes of chitin hybrid membrane carrying corneal epithelial cel s in the rabbit corneal epithelial injury.METHODS:Eighteen New Zealand white rabbits were enrol ed and made into left corneal epithelial injury models, and then randomized into two groups and treated with chitin hybrid membrane carrying corneal epithelial cel s (experimental group) and chitin hybrid membrane (control group), respectively. The damage area, histological changes and ultrastructure of the cornea were observed at 1, 3, and 7 days after implantation. RESULTS AND CONCLUSION:Damage area of the cornea in the experimental group was significantly less than that in the control group at 1 and 3 days after implantation (P<0.05), and the cornea in both two groups healed wel at 7 days after implantation. At 7 days after implantation, in both two groups, the corneal epithelium with six layers adhered to the corneal stroma closely, which was repaired completely and regularly. Comparatively speaking, the cornea in the experimental group possessed smooth outer layer. Besides, in the experimental group, the hexagonal corneal epithelial cel s arranged closely with flat surface;while the hexagonal corneal epithelial cel s in the control group showed no smooth surface and gaps between cel s. These results indicate that chitin hybrid membrane carrying corneal epithelial cel s promotes the repair of rabbit corneal epithelial injury.
8.Effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea
Jing LU ; Shike WU ; Guang CHEN ; Yue ZHAO ; Dan LI
Chinese Journal of Tissue Engineering Research 2016;20(52):7803-7808
BACKGROUND:Chitosan nanoparticles-encapsuled sodium hyaluronate is an effective drug for the burned cornea. OBJECTIVE:To verify the effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea. METHODS:Thirty Sprague-Dawley rats were randomly divided into three groups, and the model of burned cornea caused by base was established in the rats of model and experimental groups, fol owed by respectively treated with 10μL sodium hyaluronate/chitosan nanoparticle suspension and normal saline, once daily, for consecutive 4 weeks. Rats only given normal saline were used as controls. Four weeks later, the dynamic growth of newly formed blood vessels in the cornea was observed using silt lamp. The levels of tumor necrosis factor-αand interleukin-6 were detected by ELISA, histological changes of the cornea were observed by hematoxylin-eosin staining, and the mRNA expression levels of vascular endothelial growth factor and cyclooxygenase 2 were detected by real-time PCR. RESULTS AND CONCLUSION:Compared with the control group, the area of the newly formed blood vessel and the levels of tumor necrosis factor-α, vascular endothelial growth factor and cyclooxygenase 2 were significantly increased in the model group (P<0.05, P<0.01). In the experimental group, al above indicators were significantly lower than those in the model group (P<0.05). There were a large number of inflammatory cel s and neovascularization in the model group, but only few inflammatory cel s in the experimental group. These results show that sodium hyaluronate/chitosan nanoparticles can inhibit the neovascularization in the burned cornea.
9.Analysis of the related factors of periodontitis in Ningbo petrochemical company staff
Hengbiao PAN ; Hui CHEN ; Dan JIN ; Jing ZHANG
Chinese Journal of Primary Medicine and Pharmacy 2013;20(1):23-25
Objective To find out the prevalence of periodontitis in a petrochemical industry community in Ningbo ; to identify the possible factors of the prevalence; to evaluate the impact of the polluted environment on the oral health of the employees;and to provide information to the enterprises in oral diseases prevention.Methods 2 108 peoples who received regular physical examination in a petrochemical industry community in Ningbo was included.To each subject,a periodontal examination according to the principles and fundamental methods which were used in the third national oral health epidemical survey was carried out,the fast blood was phlebotomized,total cholesterol(TC),triglyceride (TG),uric acid,blood glucose and other serum indexes levels were recorded.These subjects were stratified into two age groups.Data was analyzed with x2 test and multiple regression.Results In the group of 2 108 selected subjects,the periodontitis rate was 52.3 % [95 % CI:(52.3 ± 2.1) %].In the multivariable logistic regression model,the old-age groap age(OR =4.783,P =0.000),blood pressure (OR =1.526,P =0.001) and blood glucose (OR =1.560,P =0.045) were the risk factors of periodontitis,while the environmental factor(OR =0.661) was excluded.In the young and middle-age group,blood pressure (OR =2.184,P =0.000),blood glucose (OR =2.314,P =0.001),TC(OR =1.356,P =0.003),and alcohol (OR =1.382,P =0.02) were the risk factors of periodontitis.Conclusion The levels of blood glucose,TC,blood pressure and alcohol should be considered in periodontitis prevention and the sulfureted hydrogen-based environment factor had no adverse impact on periodontitis.
10.Evaluation and analysis of transvaginal three-dimensional ultrasound in late pregnancy women of pelvic hiatus morphological structure
Jing CHEN ; Qing HUANG ; Dan LIAN ; Liuyu JI ; Hongfeng QIU
Chongqing Medicine 2015;(36):5084-5086
Objective To evaluate and analyse the morphological structure in late pregnancy women of pelvic hiatus by appli-cation of transvaginal three-dimensional ultrasound .Methods The 60 cases pregnant women who were checked pregnant weeks for 31 to 40 weeks from January 2012 to December 2014 came to our hospital were chosen as the observation group ,and other 60 cases women who were nulliparous women because of irregular menstruation at the same period treatment as control group .All patients underwent transvaginal three-dimensional ultrasound examination by observed the morphological structure of pelvic hiatus in each period ,and the research object of pelvic hiatus left and right diameter ,anteroposterior diameter and area were compared and meas-ured between the two groups .Results (1)The normal pelvic hiatus morphology was rhombic column crack-like structure ,but the observation group who had 43 .33% abnormal pelvic hiatus morphology and there was kind oval ;(2)The observation group were pelvic diaphragm hiatus of left and right diameter ,anteroposterior diameter and area were significantly larger than the control group in three conditions of the resting period ,anal contraction period and tension period ,and the difference was statistically significant(P< 0 .05) ;all the research object of the pelvic diaphragm hiatus left and right diameter ,anteroposterior diameter and area were de-crease in the anal contraction and increases in tension period for compared with the resting period ,so the difference was statistically significant (P< 0 .05) .Conclusion The three-dimensional ultrasound can be clearly observed the morphology of the female pelvic diaphragm hiatus ,and can be effectively evaluated .The late pregnancy women who will be significantly increases of pelvic dia-phragm ,occurs the relaxation phenomenon and some abnormal morphology for compared with nulliparous women .