2.Effects of isoliquiritigenin on cognition dysfunction and brain energy metabolism following repeated cerebral ischemia-reperfusion in mice
Chun ZHAN ; Jing YANG ; Li ZHAN ; Lin ZHANG ; Jing ZHANG
Chinese Pharmacological Bulletin 1987;0(02):-
Aim To investigate the effects of isoliquiritigenin(ISL) on cognition dysfunction and brain energy metabolism following repeated cerebral ischemia-reperfusion in mice.Methods Mouse models of learning and memory deficits were made by repeated occlusion of bilateral common carotid arteries and reperfusion. Step-down test and Y-maze task were used to examine the learning and memory abilities of mice. The brain energy state was analyzed by reversed-phase high performance liquid chromatogra phy. Results ISL 10, 20 and 40 mg?kg -1 ig reduced the n umber of errors and extended the latency of memory in step-down test, decreased the number of cumulated stimulation and increased the number of right choices i n Y-maze task. ISL(10, 20, 40 mg?kg -1) increased the levels of ATP, ener gy charge (EC) and total adenine nucleotides(TAN) in a dose-dependent manner. Conclusion The results suggest that ISL improves cognition dysfun ction induced by repeated cerebral ischemia-reperfusion in mice, which may be r elated to the improvement of brain energy metabolism.
5.Gladness and troubles for using garlic as an anti-oxidant.
Chinese Journal of Integrated Traditional and Western Medicine 2004;24(5):462-463
Allyl Compounds
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chemistry
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pharmacology
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toxicity
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Animals
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Antioxidants
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chemistry
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pharmacology
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toxicity
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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toxicity
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Garlic
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chemistry
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toxicity
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Humans
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Phytotherapy
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adverse effects
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Sulfides
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chemistry
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pharmacology
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toxicity
8.Effect of Both Limited Selective Posterior Rhizotomy and Limited Selective Soft Tissue Release Treating Spasmo-Cerebral Palsy in Both Legs
zhan-chun, LI ; jing-dong, ZHANG ; xin, ZHANG
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To analyze the effect of both limited selective posterior rhizotomy(LSPR)and limited selective soft tissue release(LSTR)on healing motor function related to spasmo-cerebral palsy in both legs.Methods Thirty-eight cases CP with LSPR and LSTR were collected in the past 3 years;21 males,17 females;aged 3.0-8.11 years old,on the average of 5.11 years old.Perioperative motor function were evaluated according to motor function scores.Results LSPR and LSTR played a role in improving motor function obviously,and had little difference in orthomorphia after SPR for 6 months.Conclusion LSPR and LSTR may improve cramp and abnormality in both legs,and enhance motor function,so the secondary operation agony can be avoided,and the family economic burden relived.
9.Preparation of a novel AAV-ITR gene expression mini vector in Sf9 insect cells via baculovirus.
Taiming LI ; Junjie PAN ; Jing QI ; Chun ZHANG
Chinese Journal of Biotechnology 2015;31(8):1230-1238
AAV-ITR gene expression mini vector is a double-strand or single-strand DNA that only contains inverted terminal repeats of adeno-associated virus, cis-elements and gene of interest and does not contain any other foreign DNA sequences. We prepared Bac-ITR-EGFP and Bac-inrep. Spodoptera frugiperda cells were infected with Bac-ITR-EGFP (P3) and Bac-inrep (P3). Up to 100 μg of AAV-ITR-EGFP gene expression mini vectors were extracted from 2 x 10(7) cells of Sf9 72 h after infection. The gel electrophoresis analysis shows that most forms of AAV-ITR-EGFP gene expression mini vector were monomer and dimer. The mini vector expression efficacy was examined in vitro with HEK 293T cells. The EGFP expression was observed at 24 h after transfection, and the positive ratio reached 65% at 48 h after transfection.
Animals
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Baculoviridae
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DNA, Single-Stranded
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Dependovirus
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Gene Expression
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Genetic Vectors
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HEK293 Cells
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Humans
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Sf9 Cells
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Terminal Repeat Sequences
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Transfection
10.Study of gene expression of heat shock protein70 induced by lead.
Jing ZHANG ; Ke-Ming LIU ; Chun-Hua WANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2008;26(12):752-754
Animals
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Cells, Cultured
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Female
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HSP70 Heat-Shock Proteins
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genetics
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metabolism
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Humans
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Lead
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pharmacokinetics
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toxicity
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Liver
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metabolism
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Male
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RNA, Messenger
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genetics
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Rats
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Rats, Wistar