Objective: To study the effect of Jiegusan on bone metabolism in patients with humeral fracture. Methods: Totally 128 patients with humeral fracture were divided into the treatment group and control group, and then to observe the influence of Jiegusan on bone metabolism in the patients. Results: Symptoms such as swelling and pain in treatment group were significantly improved after treatment with Jigusan than control group. After treatment, serum osteocalcin and alkaline phosphatase in the treatment were significantly higher (P < 0.05) and serum D-dimer production was significantly lower than that in the control group, while serum calcium level in serum showed no difference between the experimental and control group. Conclusion: Jiegusan can accelerate fracture healing via regulating the bone metabolism and play a role in anticoagulant activity via lowering the level of serum D-dimer.

Objective: To develop an HPLC-DAD method for the simultaneous determination and comparative analysis on the contents of prim-O-glucosylcimifugin, 4′-O-β-D-glucosyl-5-O-methylvisamminol, cimifugin, sec-O-glucosylhamaudol, psoralen, imperatorin, bergapten, and xanthotoxin in the roots of Saposhnikovia divaricata from Longxi areas. Methods: The extracts were obtained by methanol reflux method and the contents of the eight compounds were determined by HPLC-DAD. The mobile phase, consisting of acetonitrile-water, was programmed for a gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 254 nm, and the column temperature was 25°C. Results: Excellent linearity with correlation coefficents (r) of 0.9992-0.9999 was obtained. The average recoveries of the eight compounds were 96.2%-104.1% and all RSD values were less than 3%. The contents of the four coumarins in the roots of S. divaricata were much less than those four chromones. The contents of the four coumarins in the roots of Carum carvi and Peucedahum ledebourielloides were more than those in S. divaricta, while no chromones were detected except sec-O-glucosylhamaudol in P. ledebourielloides. Conclusion: The method appears to be simple, accurate, and well reproducible, which could be used for the simultaneous determination of the above-mentioned eight compounds in S. divaricata. According to the above analysis, C. carvi and P. ledebourielloides could not be used as the succedaneum of S. divaricata on the basis of the eight compound contents.

Objective: To study the correlation between the content of volatile oil and the rhizosphere soil from the roots of Saposhnikovia divaricata collected from different regions at different harvest time. Methods: The volatile oil from the roots of S. divaricata was extracted by steam distillation and analyzed. According to the National Standard of the People's Republic of China, we determined the pH value, total nitrogen, hydrolysable nitrogen, available phosphorus, available potassium, and organic matter in the rhizosphere soil of S. divaricata. The obtained data were statistically analyzed with SPSS 13.0 software. Results: The content of volatile oil in the roots of S. divaricata presented a trend of decrease with different collection regions from south to north and the main chemical components were significantly different, in which 1-(1-formylethyl)-4-(1-buten-3-yl)-benzene and β-bisabolene were found as the principal components. There was a significantly negative power function relationship between the content of volatile oil and the total nitrogen, a significantly non-linear correlation between the content of volatile oil and the hydrolysable nitrogen, but a good correlation between content of volatile oil and available phosphorus, available potassium, and the content of organic matter was found only in the first sample. There were significant good correlations of the pH value and hydrolysable nitrogen with available phosphorus, hydrolysable nitrogen, and available phosphorus with available potassium, hydrolysable nitrogen, available phosphorus, and available potassium with organic matter in rhizosphere soil (P < 0.01). The significant correlation of hydrolysable nitrogen, available potassium with pH was found (P < 0.05). Conclusion: There is no significant difference among the contents of volatile oil extracted from S. divaricata collected at different harvest time. There is a negative correlation between the total nitrogen content in rhizosphere soil and the content of volatile oil, namely, the productivity of volatile oil decreases with the increase of total nitrogen content, while the optimal contents of hydrolysable nitrogen, available phosphorus, available potassium, and organic matter could accumulate the content of volatile oil. On the other hand, pH value may affect the content of volatile oil by modulating the other chemical properties of the rhizosphere soil.

Objective To investigate the correlation between blood routine test indicators and advanced saphenous vein graft disease (SVGD) in patients with coronary artery bypass grafting (CABG). Methods By defining SVGD as an occlusion of 50% or more of the saphenous vein graft (SVG) excluding distal anastomotic occlusion, patients were divided into SVGD group and non-SVGD group, who suffered CABG over 1 year with recurrent angina and underwent coronary angiography (CAG) operation from March 2015 to January 2016 in Tianjin Chest Hospital. Results of blood routine test data were compared between two groups. The multivariable Logistic regression was analyzed for the relationship between blood routine test indicators and advanced SVGD. Results There were 148 patients in the study, 109 patients in SVGD group and 39 patients in non-SVGD group. There were significant differences in level of red blood cell distribution width (RDW:0.123 2 ± 0.008 9 vs. 0.120 2 ± 0.005 2, P<0.05), neutrophil/lymphocyte ratio (NLR:3.40 ± 2.11 vs. 2.75 ± 0.82, P<0.05) and platelet and lymphocyte ratio (PLR:143.10 ± 54.70 vs. 124.57 ± 34.40, P<0.05) between SVGD group and non-SVGD group. Multivariable Logistic regression analysis showed that RDW>0.127 5[OR (95%CI):4.905 (1.058-22.747), P=0.042], NLR>3.34[OR(95%CI):4.013(1.466-10.987), P=0.007]were independent risk factors for advanced SVGD, as well as PCT>0.185 [OR(95%CI):2.636(1.098-6.324), P=0.030]might be risk factor for advanced SVGD. Conclusion RDW>0.127 5, NLR>3.34 could indicate advanced SVGD. We need more samples to support that PCT>0.185 is used to be risk indicators for advanced SVGD.

OBJECTIVETo study the promoting effects of the epidermal growth factor (EGF) and stem cell factor (SCF) on the proliferation and differentiation of spermatogenic cells in mice.

METHODSWe cocultured in vitro spermatogenic cells of male mice aged 7 - 8 days in the medium with EGF and/or SCF at the concentrations of 5, 10, 20, 40 and 100 ng/ml, respectively. Then we observed the survival rate and morphological changes of the spermatogenic cells, detected the expressions of the pachytene-specific phosphoprotein gene (P19) and haploid sperm cell-specific transition protein gene (TP1), and analyzed the ploidy of the cells.

RESULTSAfter cocultured with EGF or SCF for 2 - 4 days, the spermatogenic cells began to proliferate in masses or chains in all concentration groups, most obviously in the 20 ng/ml EGF and 40 ng/ml SCF groups. At 7 days, both the number and survival rate of spermatogenic cells were significantly higher in the 20 ng/ml EGF and 40 ng/ml SCF groups than in the others (P < 0.05), and meanwhile, the P19/TP1 ratio was obviously decreased and the rate of haploid sperm markedly increased in the 40 ng/ml SCF group (P < 0.05). Combination of EGF and SCF remarkably promoted the proliferation of the spermatogenic cells (P < 0.05).

CONCLUSIONBoth EGF and SCF could increase the number and survival rate of spermatogenic cells. SCF could promote the formation of haploid sperm, and the combination of the two cytokines could enhance the effect on the proliferation of spermatogenic cells.

Animals ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; Cells, Cultured ; Chromosomal Proteins, Non-Histone ; metabolism ; Epidermal Growth Factor ; pharmacology ; Male ; Mice ; Spermatocytes ; cytology ; drug effects ; Stem Cell Factor ; pharmacology

Objective To investigate the clinical efficacy and prognosis of early protective pulmonary ventilation and intra-aortic balloon pump(IABP)combined with percutaneous coronary intervention(PCI)in treating patients with acute myocardial infarction(AMI)complicated with cardiogenic shock(CS)and hypoxemia.Methods The 49 patients diagnosed as AMI complicated with CS and hypoxemia and treated with early protective pulmonary ventilation and IABP combined with PCI in Tianjin Chest Hospital from January 2015 to December 2015 were retrospectively analyzed.Major adverse cardiovascular events(MACE)including sudden cardiac death,recurrent myocardial infarction,heart failure and development of target vessel revascularization were recorded during one year of follow-up visits.Survival rate was analyzed and Cox regression analysis was used to explore risk factors for occurrence of MACE after the treatment.Results The revascularization of target vessel was successfully operated on all of the 49 patients.The mean time from attack to hospitalization was(6.2±1.5)hours,while the mean time from emergency room to balloon dilatation was(118.55±28.28)minutes.In these patients,30(61.2%)cases were diagnosed as STEMI,among which 23(76.6%)cases as anterior wall myocardial infarction and 5(16.7%)cases as inferior wall myocardial infarction.While the other 19(38.8%)cases were diagnosed as non-STEMI.Using coronary arteriography,46(93.9%)cases had multi-vessel coronary artery disease,among which 13(26.5%)cases were complicated with severe left main coronary artery disease and 27(55.1%)cases had severe vascular calcification.44(89.8%)cases had TIMI perfusion grade 3 blood flow after PCI,while 5(10.2%)cases did not achieve TIMI grade 3 blood flow.One(2.0%)case had serious complications and 12(24.5%)patients died in hospital.31(63.3%)patients survived 1 year during follow-up visits.Total number of MACE was 32(65.3%).Among these events,18(36.7%)died,4 cases(8.2%)had recurrent myocardial infarction,13 cases(26.5%)had heart failure(HF)and 5 cases(10.2%)had target vessel revascularization(TVR).The total event-free survival rate was 34.7%.Multivariable Cox regression analysis showed that severe vascular calcification〔HR(95%CI):2.677(1.184-6.054),P=0.018〕,TIMI grade blood flow less than level 3 after PCI〔HR(95%CI):26.289(6.314-109.470),P=0.000〕and more than 120 minutes from emergency room to balloon dilatation〔HR(95%CI):2.923(1.325-6.446),P=0.008〕were risk factors for MACE.Conclusions Early protective pulmonary ventilation and IABP combined with emergency PCI are safe and effective for treatment of patients with AMI complicated with CS and hypoxemia.The pattern of treatment can significantly decrease death rate and increase 1-year survival rate.

Objective To investigate the protective effect of Ambroxol against experimental lung ischemia reperfusion (I/R) injury in a situ hilar clamp model.Method Left lung of rat was rendered and ischemic for 90 minutes,and reperfused for up to 2 hours,as the model.Twenty-four SD rats were randomly divided into 3 groups with 8 rats each group:control group,I/R group,I/R and Ambroxol treatment group (AMB group).Rats of AMB group received Ambroxol (25 mg/kg) Intraabdominally 30 minutes before ischemia and intravenously 5 minutes before reperfusion.After 2 hours of reperfusion,blood-gas analysis,the serum level of IL-1?,IL-8 and TNF-?from carotid artery were delected.The wet/dry ratio of lung,the activity of erythrocuprein (SOD),the content of malonaldehyde (MDA) and the activity of myeloperoxidase (MPO) were determined and pathematology changes were observed in the left lung tissue.Differences within the groups were analysed using two-sample t-test. Results After 2 hours of repeffusion,there were no significant changes of artery partial pressure of oxygen (PO_2) and partial pressure of carbon dioxide (PCO_2) among three groups.The wet/dry ratio of lung,the activity of MPO (U/g) and the content of MDA (nmol/mgprot) of I/R group were (5.3?0.5),(1.30?0.26) and (0.66?0.16),significantly higher than those of the control group (P

ObjectiveTo investigate the effects of Akt3 gene knockout on neuropathic pain behaviors induced by chronic constriction injury of sciatic nerve (CCI).MethodsExperiment was divided into two groups:Akt3 knockout group (Akt3-/-,n =12),wild type group (WT,n =12 ).Randomly numbered,the right sciatic nerve of mice were received the operation of chronic constriction injury.Paw withdrawal mechanical threshold (PWMT)and paw withdrawal thermal latency (PWTL) were tested on day 1 before operation and day 1,3,5,7,10,14,17,21 afterCCI.ResultsThe basic values of PWMT(right:(1.09±0.20)g,(1.17±0.22)g;left:(1.17±0.15)g,(1.22±0.23)g,P＞0.05) andPWTL(right:(6.18±1.11)s,(6.20±1.25)s;left:(5.82±0.91)s,(5.92± 1.71 ) s,P ＞ 0.05 ) had no statistically significant differences between two groups.On day 1 after operation,compared with basic values,the PWMT and PWTL of the right paw in both Akt3-/- group and WT group decreased significantly (P ＜ 0.05 ),and at least lasted up to day 21.The PWMT( 3d:(0.42 ± 0.22 ) g,(0.72 ± 0.36) g ; 17d:(0.29 ±0.15)g,(0.49 ±0.19) g;21d:(0.27 ±0.18)g,(0.56 ±0.15)g,P＜0.05) and PWTL(5d:(2.43 ±0.68)s,(3.13±0.52)s;17d:(2.43±1.26)s,(3.84±1.29)s ;21d:(2.14±1.23)s,(4.07±1.26)s,P＜0.05 ) of the right paw in Akt3-/- group was significantly lower than those in WT group.The PWMT and PWTL of the left paw in Akt3-/- group and WT group had no obvious differences (P ＞ 0.05 ). However.compared to left paw,the PWMT and PWTL of the right paw of the two groups were obviously lower (P ＜ 0.05 ).ConclusionThe neuropathic pain induced by CCI increased in Akt3 gene knockout mice.

Cytokines ; genetics ; Graft vs Host Disease ; genetics ; Hematopoietic Stem Cell Transplantation ; Humans ; Polymorphism, Genetic