Deafness ; Diabetes Complications ; diagnosis ; epidemiology ; Diabetes Mellitus ; epidemiology ; Hearing Loss ; diagnosis ; etiology ; Humans

Animals ; Apoptosis ; Caspase 3 ; metabolism ; Caspase 8 ; metabolism ; Cells, Cultured ; HSP70 Heat-Shock Proteins ; metabolism ; Heat Stress Disorders ; metabolism ; Myocytes, Cardiac ; metabolism ; Rats ; Rats, Wistar

Animals ; Cognition ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Electrocardiography ; Female ; Heart ; drug effects ; physiopathology ; Male ; Mice ; Mice, Inbred Strains ; Myocardium ; pathology ; Phytotherapy ; methods

Objective To investigate the protective effect of Ambroxol against experimental lung ischemia reperfusion (I/R) injury in a situ hilar clamp model.Method Left lung of rat was rendered and ischemic for 90 minutes,and reperfused for up to 2 hours,as the model.Twenty-four SD rats were randomly divided into 3 groups with 8 rats each group:control group,I/R group,I/R and Ambroxol treatment group (AMB group).Rats of AMB group received Ambroxol (25 mg/kg) Intraabdominally 30 minutes before ischemia and intravenously 5 minutes before reperfusion.After 2 hours of reperfusion,blood-gas analysis,the serum level of IL-1?,IL-8 and TNF-?from carotid artery were delected.The wet/dry ratio of lung,the activity of erythrocuprein (SOD),the content of malonaldehyde (MDA) and the activity of myeloperoxidase (MPO) were determined and pathematology changes were observed in the left lung tissue.Differences within the groups were analysed using two-sample t-test. Results After 2 hours of repeffusion,there were no significant changes of artery partial pressure of oxygen (PO_2) and partial pressure of carbon dioxide (PCO_2) among three groups.The wet/dry ratio of lung,the activity of MPO (U/g) and the content of MDA (nmol/mgprot) of I/R group were (5.3?0.5),(1.30?0.26) and (0.66?0.16),significantly higher than those of the control group (P

Objective To investigate the application of multi-slice spiral computed tomography(MSCT) in the diagnosis of solid pseudopapollary tumor of the pancreas (SPTP). Methods Clinical data and CT films of 12 patients with SPTP were retrospectively analyzed from January 2003 to December 2008. Results SPTP presented a typical cystic lesion with well-limited silhouette and no intensification in the cyst on enhanced CT scan. However, a slight to moderate enhancement in the solid components and a markedly enhanced envelope could be seen. Three dimensional images of MSCT can reveal clearly an anatomic relationship of the lesions with surrounding organs and blood vessels. Of 12 cases, there was one case showed that the envelope was incomplete, 3 with duodenal invasion, 2 with superior mesenteric vein involvement, and 1 with closed adhesion with spleen. All 12 patients underwent surgery and had only one tumor, tumor diameter ranged from 4 cm to 18 cm. The location of tumor in pancreas, the relation with surrounding tissue and the pathological presentation were helpful to make peroperative diagnosis. Three dimensional imaging technology of MSCT can offer important referrence for the preroperative evaluation and increase the diagnosis accuracy.

Abdominal Injuries ; complications ; Adenosarcoma ; etiology ; Adult ; Cicatrix ; complications ; Endometriosis ; complications ; Female ; Humans

Cochlear Implantation ; Humans ; Vestibule, Labyrinth ; physiology

OBJECTIVETo compare the difference in the long-term efficacy between all-trans retinoic acid (ATRA) combined Compound Huangdai Tablet and ATRA combined methotrexate (MTX) and 6-mercaptopurine (6MP) as the sequential maintenance treatment of acute promyelocytic leukemia (APL) patients.

METHODSTotally 83 APL patients in the molecular remission (PML/RARalpha negative) were randomly assigned to two groups, the treatment group (45 cases) and the control group (38 cases) after they were induced to the complete remission (CR) by ATRA combined chemotherapy, and treated by sequential chemotherapy as the consolidated treatment for 3 therapeutic courses. Those in the treatment group were sequentially treated with ATRA and Compound Huangdai Tablet as maintenance therapy, while those in the control group were treated with ATRA and MTX + 6MP as maintenance therapy. After a long-term follow-up (2003 -2011), the long-term therapeutic efficacy and adverse reactions were compared between the two therapeutic regimens.

RESULTSThe 5-year relapse-free survival (RFS) rate was 84.4% +/- 5.4% in the treatment group and 63.2% +/- 7.8% in the control group, showing statistical difference between the two groups (P < 0.05). The 5-year overall survival rate (OSR) was 86.7% +/- 5. 1% in the treatment group and 78.7% +/- 6.7% in the control group, showing no statistical difference between the two groups (P > 0.05). There was no statistical difference in the adverse reaction between the two groups (P > 0.05).

CONCLUSIONThe application of ATRA and Compound Huangdai Tablet as maintenance therapy could elevate the long-term RFS rate of APL patients.

Adolescent ; Adult ; Aged ; Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; therapeutic use ; Drugs, Chinese Herbal ; administration & dosage ; therapeutic use ; Female ; Humans ; Leukemia, Promyelocytic, Acute ; drug therapy ; Male ; Mercaptopurine ; administration & dosage ; therapeutic use ; Methotrexate ; administration & dosage ; therapeutic use ; Middle Aged ; Phytotherapy ; Treatment Outcome ; Tretinoin ; administration & dosage ; therapeutic use ; Young Adult

Objective To analyze the methylation status of MUC2 gene in pancreatic carcinoma cell lines and peripheral blood of pancreatic carcinoma patients,and to explore the role of MUC2 methylation in the early diagnosis of pancreatic carcinoma.Methods Human pancreatic cancer cell lines of SW1990,ASPC,PANC1,BxPC3,PaTu8988 and CFPAC1,and 40 peripheral blood samples of pancreatic carcinoma patients,15 cases of chronic pancreatitis,25 cases of normal controls were collected,and the methylation status of MUC2 gene was detected by methylation sensitive restriction endonuclease PCR.Results MUC2 methylation was not detected in PANC1,BxPC3,PaTu8988,but was detected in ASPC,CFPAC1,SW1990.Among the peripheral blood samples,the rate of methylation in pancreatic cancer was 40.O%(n=16),in chronic pancreatitis was 0%,in normal controls was 4.0%(n=1),and the difference among the three groups was statistically significant(P＜0.01).The methylation of MUC2 gene CpG islands for the diagnosis of pancreatic carcinoma had a sensitivity of 40%,specificity of 97.5%,accuracy of 68.8%,positive predictive value of 94.1%and negative predictive value of 61.9%.Conclusions The detection of MUC2 hypermethylation in peripheral blood samples may be an potential marker for early diagnosis of pancreatic carcinoma.

Objective To establish a system using CK activity assessing with follow-up Duchenne muscular dystrophy (DMD) gene testing to newborn screening for DMD.This study provided a pathway to improve the health outcome for individuals with DMD.Methods Tests for CK were performed with Beckman original reagent on a Beckman Coulter AU 5800.Preliminary studies established a population-based range of CK in newborns using 5 892 deidentified anonymous blood samples,which were collected from Shanghai Changning Maternity and Infant Health Hospital between November 2013 and July 2014.Mutation analysis used multiplex PCR-denature high-performance liquid chromatography (PCR-DHPLC) method for screening large duplications and deletions and Sanger DNA sequencing for screening point DMD gene mutation.Results DMD gene mutations (point mutation,exon60,c.9072G ＞ A) were found in 1 of 5 892 newborn subjects,which had CK level ＞ 2 000 U/L large duplications and deletions in DMD gene were not found.Conclusions A system of analysis for newborn screening for DMD has been established.This path for newborn screening fits our health care system and minimizes the false-positive results for predicting DMD gene mutations by use of CK levels in blood