1.Observation and nursing with different intravenous depressors for perioperative hypertension in Intensive Care Unit
Bin WANG ; Jing WANG ; Yan GAO
Chinese Journal of Rehabilitation Theory and Practice 2005;11(3):237-238
ObjectiveTo observed the clinical result of 3 kinds of commonly used depressor in Intensive Care Unit (ICU) and attend to the main point in nursing.MethodsNitroglycerin, sodium nitroprosside and urapidil hiydrochloride were used among 60 patients with perioperative hypertension in ICU respectively.ResultsSodium nitroprosside showed obviously depressed result, but needs to be observed tightly. Nitroglycerin and urapidil hiydrochloride depressed the blood pressure gentlely, and were suitable to old people particularly.ConclusionThe nurse can carry on corresponding clinical observation and nursing according to the pharmacology function of 3 kinds of medicines.
2.Adhesion of Lactobacillus protects the enterocyte-like cell HT-29 against the invasion of enteropathogenic E. coli
Bin WANG ; Guorong DAN ; Jing YUAN
Medical Journal of Chinese People's Liberation Army 1981;0(06):-
Objective To investigate the beneficial effect on enterocyte-like cells HT-29 through adhesion of Lactobacillus. Methods Using viable bacteria counting method to evaluate inhibition of Escherichia coli adhesion and invasion to enterocyte-like cells by incubation with Lactobacillus together. Cellular membrane permeability and cell viability were examined in vitro by LDH level and MTT method respectively. Results Adhesion and invasion of enteropathogenic Escherichia coli to enterocyte-like cells was inhibited by high adhesiveness strain Lactobacillus reuteri JCM1081. In vitro, no significant changes of the morphology, structure and function of the HT-29 cells after being treated with Lactobacillus alone for 3 hours, especially cellular LDH level, viability and membrane permeability. Lactobacillus reuteri JCM1081 did not alter cell integrity and prevented the increase in permeability induced by enteropathogenic Escherichia coli infection. Conclusion Lactobacillus reuteri JCM1081 interact with intestinal epithelial cell receptor to competitively inhibit the adhesion and invasion of enteropathogenic Escherichia coli to HT-29 cells by incubation and enhance the integrity of the cells.
3.Stationary cycle training for improving lower limb muscle tension and motor function in children with spastic cerebral palsy
Jing GAO ; Lina WANG ; Bin ZHAO
Chinese Journal of Physical Medicine and Rehabilitation 2015;37(6):432-435
Objective To observe the effects of stationary cycle training on lower limb muscle tension and motor function in children with spastic cerebral palsy.Methods Sixty-seven children with spastic cerebral palsy were divided into a treatment group (n =34) and a control group (n =33).Both were treated with routine rehabilitation therapy,including neurodevelopment treatment,massage and physical agent therapy.The treatment group additionally cycled on a stationary bicycle for 20 min a day,five days a week for 3 months.Muscle tension in the children's adductor,hamstring and gastrocnemius muscles was evaluated using the modified Ashworth scale (MAS),while the adductor angle,popliteal fossa angle and foot dorsiflexion angle were measured using a joint protractor.Moreover,the 88-item version of the Gross Motor Function Measure (GMFM-88) was used to evaluate gross motor function.Results After treatment the average muscle tension of both groups was significantly lower than before treatment.The average muscle tension scores of the adductors,hamstrings and calf muscles of the treatment group [(1.79±0.54),(1.00±0.60),(2.29 ±1.77)] were significantly lower than those of the controls [(1.82 ± 0.53),(1.15 ± 0.44),(2.52 ± 1.89)].The adductor angles,popliteal fossa angles and foot dorsiflexion angles of both groups were significantly larger than before treatment,and the angles in the treatment group [(97.06 ± 19.03) °,(53.38 ± 12.54) ° and (8.38 ± 9.11) ° respectively] were significantly larger on average than those in the control [(98.94 ± 21.42) °,(56.52 ± 12.90) ° and (5.30 ± 14.30) ° respectively].After treatment the total GMFM scores of both groups were better than before treatment,but the treatment group's average score(138.18 ± 54.86) was significantly better than that of the control group(112.21 ± 62.44).Conclusion Three months of stationary cycle training can effectively reduce the muscle tension of children with spastic cerebral palsy and improve their motor function.
4.Effect of adhesion of Lactobacillus to enterocyte-like HT-29 cells on filamentous cytoskeleton
Bin WANG ; Jing YUAN ; Hong WEI
Parenteral & Enteral Nutrition 1997;0(04):-
Objective: To investigate the effect of adhesion of Lactobacillus reuteri JCM1081 to enterocyte-like HT-29 cells on filamentous cytoskeleton.Methods:Cultured human intestinal enterocyte-like HT-29 cells were used.Cell permeability was examined in vitro by Evans blue labeled albumin and Examination of F-actin was conducted by direct immuno-fluorescence labeling using fluorescein labeled phalloidin.Results:Lactobacillus reuteri JCM1081 did not alter cell integrity and prevented the increase in permeability induced by enteropathogenic Escherichia coli infection.The distribution of F-actin were also not altered.Conclusion :Lactobacillus reuteri JCM1081 exerts a protective effect against the filamentous cytoskeleton rearrangement and permeability lesions promoted by enteropathogenic Escherichia coli infection.
5.The Importance of Properly Understanding SCI from the Perspective of Education for PhD Students in Medicine
Li-Ru WANG ; Bin SHI ; Jing LI ;
Chinese Journal of Medical Education Research 2006;0(10):-
Doctor's degree is the highest degree for medical education.Nowadays,medical doctor candidates in more and more medical universities are requested to publish research papers in intemational journals embodied in Science Citation Index(SCI),or they can not graduate with doctor's degree.So,it is significant for medical doctor candidates to learn the related knowledge about SCI and Impact Factor(IF).
6.Isobaric tags for relative and absolute quantitation for preliminarily screening of differential proteins in hippocampus of Ts65Dn mice
Bin YU ; Bin ZHANG ; Jing KONG ; Qiuwei WANG ; Shihe SHAO
Chinese Journal of Perinatal Medicine 2013;16(12):770-773
Objective To explore the molecular mechanism of hypophrenia induced by Down syndrome (DS).Methods Ts65Dn mice were used as DS animal model.Three female mice and three male mice of three to twelve weeks old were mated.Among the 17 first-generation mice alive,five mice remained Ts65Dn trisomy and 12 mice were normal.Five Ts65Dn mice and five normal mice were selected randomly as Ts65Dn group and control group,and bred till 16 to 18 weeks old for experiments.Differential proteins in hippocampus of mice were tested by isobaric tags for relative and absolute quantitation (iTRAQ).Expressions of the differential proteins in Ts65Dn group were detected compared with those in control group.Results A total of 2805 proteins were identified in hippocampus of Ts65Dn group and control group,and significant differences were observed in the expressions of 374 proteins.Compared with those in control group,expressions of 195 proteins increased and 179 reduced in Ts65Dn group.Sorted by P value from low to high,the seven proteins with the lowest P value were uncharacterized protein C2orf47 homolog,isoform 2 of filamin A-interacting protein 1-like,zinc finger protein,isoform 1 of pericentriolar material 1 protein,SEC23 interacting protein,BAG family molecular chaperone regulator 3 and serpin H1.Conclusions Differential proteins are observed in hippocampus of Ts65Dn mice,perhaps closely correlating to neurological defects.The new technology of iTRAQ helps to screen and identify differential proteins in hippocampus.
7.Clinical features and surgical treatment of retroperitoneal Castleman′s disease with paraneoplastic pemphigus
Lanbo ZHANG ; Yinhua LIU ; Bin WANG ; Jing WANG ; Xuejun ZHU
Chinese Journal of General Surgery 1993;0(03):-
Objective To investigate the clinical characteristics and surgical treatment of retroperitoneal Castleman′s disease (CD) with paraneoplastic pemphigus (PNP).Methods The clinical symptoms, laboratory, histopathologic, CT findings and results of surgery in 7 patients were evaluated.Results All of 7 patients presented with PNP.Retroperitoneal tumors were found by CT examination and surgically removed.Castleman disease was confirmed by histopathology.Reoperation was performed to resect the recurrent tumor in one patient.The signs of PNP were dissolved completely or partially after operation.Conclusion Retroperitoneum CD with PNP are characteristic of distinctive clinical features.Surgical excision improves the mucocutanous manifestation of PNP.
8.Clinical study on Honggui capsule combined with chemotherapy for lung cancer
Bin WANG ; Xiaoyuan LI ; Yang LIU ; Fengyun ZHANG ; Jing WANG
International Journal of Traditional Chinese Medicine 2015;(11):991-993
Objective To observe the clinical efficacy of Honggui capsule combined with chemotherapy in the treatment of lung cancer. Methods 61 cases of lung cancer patients were randomly divided into group A of 30 cases, treated with Honggui Capsule and chemotherapy, and group B of 31 cases treated with chemotherapy only by the envelope method. After both groups were treated for 21 days, the clinical effect, life quality improvement, improvement of TCM symptoms and the incidence of toxic and side effects in 2 groups were compared. Results After treatment, there was significant difference between group A and B in the tumor response rate (26.7% vs. 3.2%; χ2=4.927, P=0.026), Karnofsky (23.3% vs. 3.3%; χ2=5.410, P=0.020), and improvement of TCM symptoms (13.3% vs. 0.0%; χ2=4.423, P=0.035). During treatment, there was significant difference between group A and B in bone marrow suppression (33.3% vs. 58.1%; χ2=4.778, P=0.029), and gastrointestinal disturbance (16.7% vs. 58.1%; χ2=11.124, P=0.001). Conclusion Honggui capsule combined with chemotherapy has an enhanced efficacy and a detoxicating effect, besides it improves clinical symptoms and reduces the side effects of chemotherapy.
9.Intense pulsed light and red light emitting diode for the treatment of steroid-dependent dermatitis
Jing WANG ; Bin LIU ; Qi LUAN ; Yanting WANG ; Chengxin LI
Chinese Journal of Dermatology 2012;45(3):205-207
Objective To retrospectively review the efficacy and side effects of intense pulsed light (IPL) and red light emitting diode (LED) in the treatment of steroid-dependent dermatitis.Methods Seventy patients with steroid-dependent dermatitis mainly manifesting as facial telangiectasis were treated with IPL for an average of 3.49 sessions with a 4-week interval.The energy density of IPL varied from 20 to 23 J/cm2,pulse width from 2.6 to 5.0 ms,and delay from 15 to 20 ms.Meantime,197 patients with steroid-dependent dermatitis,who mainly presented with facial skin sensitivity,were treated with red LED (633 ± 3 nm wave length) twice a week for an average of 4.23 sessions.The energy density of red LED was 128 J/cm2,and the irradiation lasted 20 minutes at each treatment.The efficacy and adverse reactions were assessed and recorded for each treatment.Results The total response rate was 88.57% for IPL,and 83.76% for red LED.There was a significant difference in the clinical efficacy between triple-pulse and double-pulse IPL (x2 =8.14,P < 0.05).No severe adverse reaction was observed in any of the patients.Conclusion IPL and red LED are both effective in treating steroid-dependent dermatitis.
10.Observation of blood fat in patients with cerebral infarction complicated by metabolic syndrome receiving community path intervention treatment
Jing WANG ; Ruxiang XU ; Bin YANG ; Fengchun LIU ; Zhizhong WANG
Chinese Journal of Postgraduates of Medicine 2013;36(25):32-36
Objective To explore the changes of blood fat in patients with cerebral infarction complicated by metabolic syndrome (MS) receiving community path intervention treatment.Methods A total of 116 cases of cerebral infarction complicated by MS were selected and given comprehensive intervention treatment after risk assessment.Patients were divided into intervention group (60 cases) and control group (56 cases) according to their difference in compliance.Results After intervention,the total cholesterol (TC),triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) in two groups were significantly decreased,high-density lipoprotein cholesterol (HDL-C) was significantly increased.There were significant differences in intervention group before and after intervention (P< 0.05).Compared with those in control group,differences of all index in each time point in intervention group were statistically significant (P <0.05).There was no significant difference in carotid plaque integral before intervention between two groups (P> 0.05).The carotid plaque integral 12 and 24 months after intervention in intervention group was significantly lower than that before intervention (P < 0.05).The carotid plaque integral 12 and 24 months after intervention in intervention group was significantly lower than that in control group [(3.20 ± 2.01) cm vs.(4.71 ±2.87) cm,(2.98 ±2.61) cm vs.(4.60 ±2.43) cm,P<0.05].Twelve and 24 months after intervention in intervention group,TC and carotid plaque integral was significantly positive correlation (r =0.304 and 0.317,P < 0.05),TG and carotid plaque integral was significantly positive correlation (r =0.229 and 0.128,P < 0.05),LDL-C and carotid plaque integral was significantly positive correlation (r =0.654 and 0.518,P < 0.05),and HDL-C and carotid plaque integral was significantly negative correlation (r =-0.495 and-0.528,P < 0.05).Conclusion For patients of cerebral infarction complicated by MS,great importance should be attached to early prevention and control of their major components so as to reduce the incidence of acute cerebrovascular recurrence and mortality.