Background Keratectasia after laser in situ keratomileusis (LASIK) is one of the serious complications which affect the vision.The measurement of cornea posterior elevation is an important way helping to find this change.Objective The aim of this study was to investigate the change of cornea posterior elevation after LASIK in myopic eyes with astigmatism and affecting factors.Methods A series case-observational study was adopted.One hundred and twenty-seven myopic eyes with astigmatism of 66 patients who received LASIK in 2008 May through 2010 January in Beijing Tongren Hospital were retrospectively analyzed to evaluate the change of cornea posterior elevation following the LASIK.The parameters related to cornea posterior elevation were measured and compared before and 3 months,6 months and 1 year after surgery,respectively,with Oculyzer anterial segment analysis system.Results The height values from posterior cornea highest point,the lowest point and central vertex central elevation zone were (12.20±3.39),(-19.02±7.38) and (1.05 ±3.25) μm respectively before LASIK and were (14.38±3.80),(-18.55±7.11),(2.83±4.81)μm in3 months and (13.99±3.38),(-17.57±6.54),(2.45±4.61) μm in 6 months after LASIK.They were (14.40±3.85),(-17.76±6.00),(2.16±5.00) μm in 1 year after surgery.Significant increases were found in the vertex height and central elevation after LASIK compared with before surgery(highest pointq=6.813,5.594,6.875,all at P＜0.001.central vertex:q=4.488,P=0.002;q =3.530,P=0.013 ;q =2.799,P =0.047).However,no significant difference was seen in various time points after LASIK (P＞0.05).A positive correlation was obtained between the height value of the posterior cornea central vertex with the spherical equivalent (SE),maximum cutting depth or cutting proportion 1 year after LASIK (r =0.295,0.297,0.295,all at P=0.001),and there was a negative correlation between it with residual stroma (r=-0.208,P=0.019).The intraocular pressure (IOP) measured with noncontact tonometry (NCT) was (14.24±3.33) mmHg before LASIK,and those of 3 months,6 months and 1 year were (8.42± 1.90),(8.61 ± 1.64) and (8.76± 1.64)mmHg after LASIK,showing a significant lowing in IOP after LASIK in comparison with before LASIK (q =29.851,28.317,26.337,all at P＜0.001).But no significant change was found in the different time points after LASIK (P＞0.05).There was significant difference in the IOP corrected by Ehlers after LASIK (P＞0.05).The IOP before LASIK had positive correlation with the central elevation of posterior cornea surface 1 year after LASIK(r=0.258,P =0.003).Conclusions The cornea posterior elevation increases slightly early stage after LASIK but retains stable level with lapse of time.The refraction diopter before LASIK is the main factor affecting the posterior corneal shape.To reduce the risk of corneal ectasia,more attention should be paid to the reserving of appropriate corneal residual stroma and smallest degree of eccentricity during the ablation procedure.

Objective:To find out the characteristic topog ra phic distribution of the muscle spindles in human masseter.Methods: The muscle spindles of masseter on both sides of 5 human corpses fixed in for malin for more than 1 year were observed by histological technique and quantitat ively analyzed by picture analysis equipment.Results: The muscle spindles were maldistributed and the spindles were commonly clustered in some i ndividual portions,and the muscle spindles figures of the masseter were diversif orm,which were predominantly composed of the spindle simple and spindle complexe s. The spindle complexes in the deep portion was obviously more than that in the superficial. The muscle spindles in the muscle close to the temporomandibular j oint and muscle tendon in the deep portion were more than that in the other port ions.Conclusion:The distribution of muscle spindles in the masse ter is heterogeneous,muscle spindle density in the deep portion is greater than that in the superficial.

Bacteriophage is a kind of virus depending on bacterium,named bacterial virus,and it can multiply in bacterium.There're six types of Chlamydiophage discovered which are Chp1,Chp2,Chp3,Chp4,CPAR39 and PhiCPG1.Capsid proteins Vp1,Vp2 and Vp3 are three major structural proteins of Chlamydiophage.The study of Chlamydiophage will play great action on chlamydia infection therapy.

Objective To improve the effects of health education(HE)and satisfaction degree on HE of inpatients by executing hierarchical full-responsibility nursing. Methods Fourteen wards were randomly chosen form the hospital and divided into the control group and the experimental group, each group having 7 wards. The control group carried out routine holistic nursing model, and the experimental group carried out a new hierarchical full-responsibility nursing system. Reforming nursing scheduling and diminishing the nursing unit to assure that the patients acquired the continous and stable nursing service when they were in hospital. The effect and the degree of satisfaction of HE were compared between two groups after 6 months. Results The effects of HE in the experimental group were significantly better than those in the control group, and the degree of satisfaction on HE in the experimental group was much higher than in the control group. Conclusions The hierarchical full-responsibility nursing system can give patients systematic and normalized HE. It can significantly improve the effects and the degree of satisfaction on HE.

Adenocarcinoma ; genetics ; metabolism ; Angiomyolipoma ; etiology ; Antibiotics, Antineoplastic ; therapeutic use ; Astrocytoma ; etiology ; Brain Neoplasms ; etiology ; Breast Neoplasms ; genetics ; metabolism ; Gene Expression Regulation, Neoplastic ; Genes, Tumor Suppressor ; Humans ; Kidney Neoplasms ; etiology ; Lung Neoplasms ; genetics ; metabolism ; Mutation ; Sirolimus ; therapeutic use ; TOR Serine-Threonine Kinases ; antagonists & inhibitors ; metabolism ; Tuberous Sclerosis ; complications ; drug therapy ; genetics ; metabolism ; Tumor Suppressor Proteins ; genetics ; metabolism

BACKGROUND: The conical telescopic crown denture has a better long-term effect on dentition defects of few remaining teeth than the traditional removable denture. However, there are few studies to analyze the application of these two kinds of repair methods using the finite element method. OBJECTIVE: To compare the stress distribution of the isolated abutment tooth repaired using the conical telescopic crown denture and traditional removable denture. METHODS: Two-dimensional images of the mandible and dentition in healthy adults were scanned by CT. Three-dimensional finite element models of conical telescopic crown denture and traditional removable denture were established in the mandible only with the bilateral canines. Under the same vertical and oblique loadings, the maximum equivalent stress of the abutment, periodontal membrane or edentulous alveolar ridge was compared between two repair methods. RESULTS AND CONCLUSION: Under the vertical and oblique loadings, the total stress of abutment and periodontal membrane in the traditional removable denture was higher than that in the conical telescopic crown denture and the stress in the corresponding ring was concentrated obviously; the total stress of edentulous alveolar ridge in the conical telescopic crown denture was slightly larger than that in the traditional removable denture, but the stress was wel -distributed and concentrated in a smal area. Under the oblique loading, the total stress of abutment, periodontal membrane and edentulous alveolar ridge was higher than that under the vertical loading in these two dentures, and the stress was concentrated in the periodontal membrane of lingual neck, but the total stress in the traditional removable denture was larger, with a wider concentration range. These findings indicate that the stress on the supporting tissue of conical telescopic crown denture is more evenly distributed within a smal er range than that of traditional removable denture, and the former is more fit for the health of abutment, periodontal tissues and edentulous alveolar ridge.

Objective_To evaluate the proangiogenesis of extracelluar matrix proteins SRPX2 on HUVECs.Meth-ods_pcDNA3.1-SRPX2 vector ( transfection group) and pcDNA3.1 vector ( negative group) were transfected into HEK293T cells, then divided into 3 groups including blank group.The proliferation of HUVECs ( absorbance, A450 ) was detected by CCK-8 kit.The transmembrane cell number was counted by Transwell migration and wound healing assay to evaluate the migration ability of HUVECs.A three dimensional culture system of cells was construc-ted on the Matrigel, and tube formation number of HUVECs was assessed.Results_The proliferation of HUVECs ( absorbance,A450 ) among transfection group,negative group and blank group failed to show significant difference (P>0.05).A signifiant difference was noted in the total branch point of capillary tubes among transfection group, negative group and blank group[(97 ±4)/field versus (57 ±3) and (54 ±3)/field] (P<0.05).In wound healing
assay, the distance of transfection group compared to 0, 6 and 12 h were both significantly larger than that of nega-tive group and blank group [(90 ±6), (37 ±7),(36 ±4)μm and (135 ±5),(65 ±8),(63 ±4)μm respective-ly, both( P<0.05) ] .In Tranwell assay, the number of migrating cells in the transfection group was significantly more than negative group and blank group [(549 ±10)/field vs (334 ±11) and (329 ±12)/field(P<0.05)] af-ter co-culture 16 h.Conclusions_SRPX2 may promote angiogenesis by stimulating the migration and tubing on the Matrigel of HUVECs.

Objective To investigate the influencing factors and improve potency methods of chymosin, to verify the stability and applicability of the national standard of chymosin.Methods The effects of different formula milk powder substrate and enzyme concentration on the determination of the activity of chymosin were studied.3 ×3 dose-response parallel line method was established.The results were compared with the different methods of absolute and relative methods.Results The different formula milk powder had a significant effect on the determination of the absolute potency of the activity of chymosin.The concentration of the enzyme was a power function relationship with the milk clotting time.Compared with the absolute potency, reproducibility of the relative potency of the results was better in different laboratories.The suitable doses in 3 ×3 dose-response parallel line method were 0.35,0.44,0.55U/mL.The confidence limit rate was less than 5%.The potency of the national standard of chymosin (140712-201302) was not significantly different between 2013 and 2015.In a certain dose range, the dose-response of the national standard of chimosin and gastropylor complex or lamb'tripe extract was linear, and the two lines were parallel.Conclusion A lot of factors can affect on the potency of chymosin.Relative potency is determinate by reference standard which can eliminate the influence of different substrates, different operators and endpoint judgment on the determination in order to make results have comparability between laboratories.The test design of 3 ×3 dose-response parallel line can control the test deviation better than the single point determination.The stability of the national standard of chymosin(140712-201302) is good, and is suitable for the potency of chymosin of the products of gastropylor complex and the extract of the lamb.

Objective To establish the chromogenic substrate method to determine the activity of cytochrome C.Methods Used TMB as the chromogenic substrate, reacted at 37 ℃ for 15 min, generated the yellow products, and detected the absorbance at 450 nm.The experimental design method is the 4 ×4 parallel line quantitative analysis.ResuIts The activities of cytochrome C injection samples have been determined.The linear regression equation was Y=0.9875 X -1.0221,R2 =0.9996.The accuracy and repeatability were 1.1 % and 3.6 %.ConcIusion The chromogenic substrate method was simple operation, sensitive and can be used to determine the activity of cytochrome C.

To study the cost-effectiveness of temozolomide combined with radiotherapy in the treatment of glioblasto-ma. Methods:According to the clinical trial data, cost-effectiveness and sensitivity of the results was analyzed based on the domestic cost and consumption level. Results:Temozolomide combined with radiotherapy could prolong one month of overall survival with the additional cost of RMB 58 959. 7 yuan in each case when compared with radiotherapy alone. Conclusion:Temozolomide combined with radiotherapy has no advantage on cost-effectiveness when compared with radiotherapy alone.