Objective To investigate the role of platelet rich plasma (PRP) on free fat graft's survival in a rat model.Methods Twenty-four rats were used in the experiment.For each rat,three groups of free fat grafts were injected into the subcutaneous layer of dorsum.Group A was the control group with 0.5 ml fat and 0.12 ml normal saline.Group B received 0.5 ml fat and 0.1 ml PRP with 0.02 ml normal saline.Group C was given combination of 0.5 ml fat and 0.1 ml PRP which was activated by 0.02 ml calcium chloride.Eight rats were euthanised using pentobarbital sodium each time,1,2 and 3 months after fat transplantation.Specimens were acquired and stained with haematoxylineeosin,and then the histological changes examined using light microscopy.All the data were statistically analysed using paired t test to determine if there were differences between the study groups.Results Compared the volume of the fat grafts at 1 month,there was no deference between the groups(P＞0.05).However,the diameter of group C was significantly higher than group A (P＜0.05).On histological examination,larger vascular density was observed in group C compared with group A at 1 month (P＜0.05).And reduced inflammatory reaction was also observed in group C at 3 months(P＜0.05).While other histological parameters were not statistically significant (P＞0.05).Conclusions PRP does not enhance the survival of fat graft in the Wistar rat model.

BACKGROUND:The degradation of extracellular matrix, which is mediated by matrix metal oproteinases, plays a crucial role in the corneal neovascularization. Tissue factor pathway inhibitor 2, a new type serine proteinase inhibitor, can effectively inhibit the activity of matrix metal oproteinases.
OBJECTIVE:To elucidate the effect of tissue factor pathway inhibitor 2 on the expressions of matrix metal oproteinases in keratocytes in vitro.
METHODS:Rabbit keratocytes were primarily cultured and subcultured in vitro. Plasmid vector pBos-Cite-neo/TFPI-2 was transfected into keratocytes with Lipofectamine 2000. The positive cells were selected using G418.
RESULTS AND CONCLUSION:The results of reverse transcription-polymerase chain reaction, western blot analysis and gelatinase zymography analysis showed that, expression of mRNA and protein of tissue factor pathway inhibitor 2 was upregulated in the transfected keratocytes (P<0.05), while activity of matrix metal oproteinase 1 and 2 was significantly decreased (P<0.05). Experimental findings indicate that that tissue factor pathway inhibitor 2 strongly inhibits the activity of matrix metal oproteinase 1 and 2 in keratocytes.

OBJECTIVE:To explore the role of clinical pharmacists in the therapy for patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD) complicated with allergic bronchopulmonary aspergillosis (ABPA). METHODS:Clinical pharmacists participated in the therapy for a AECOPD patient with ABPA. According to patient's con-dition,clinical pharmacists suggested adjusting the inhalation order of salbutamol and budesonide aerosol inhalation;stop-ping cefatriaxone and adjusting to intravenous injection of voriconazole;and then adjusting voriconazole to oral dosage form timely,monitoring therapy duration. Clinical pharmacists paid close attention to therapeutic efficacy and ADR,and provided medication education for patients. RESULTS:Physicians adopted the suggestion of clinical pharmacists to adjust inhalation order of aerosols,the dosage and route of administration of voriconazole. The patient's condition was improved obviously,and the drug therapy was continued after discharge. CONCLUSIONS:Clinical pharmacists participating in the whole process of treatment improves therapeutic efficacy,reduces the occurrence of ADR and promotes rational drug use in clinic.

Diabetic cardiomyopathy(DCM) is a disease independently related with coronary artery atherosclerotic heart disease, hypertensive heart disease, valvular heart disease, congenital heart disease and other heart diseases that are uniquely associated with diabetes mellitus, and the pathological manifestations of DCM are mainly myocardial hypertrophy, interstitial fibrosis, necrosis and apoptosis.Endoplasmic reticulum is the central location of many important cellular functions, and endoplasmic reticulum swelling and functional disorder in diabetic cardiomyocytes, high blood sugar can cause endoplasmic reticulum stress, suggesting that endoplasmic reticulum stress(ERS) may be involved in the pathogenesis of diabetic cardiomyopathy and development.Based on this, this paper summarizes the progress of ERS in diabetic cardiomyopathy from the pathogenesis of diabetic cardiomyopathy, unfolding protein reaction and the related role of ERS in DCM.

Environmental Microbiology is an important basic course of Environmental Engineering.Its characteristic is content broad,quick development and strong practicality,thus this curriculum's teaching has certain degree of difficulty.Some suggestions and concrete measures about teaching reform,which included curriculum's course content,teaching method,experiment teaching and assessment methods were proposed in this paper.

Objective To study the value of upbuilding heartcare network in metabolic syndrome.Methods Body mass index,waist and breech circumference,waist hip ratio,blood pressure,fasting plasma glucose,lipid profile, plasma insulin,and urine albumin were determined.Various risk factors were assessed and intervention measures were made.Results Various risk factors in metabolic syndrome were decreased after treatment intervention for 3,6 months,1 and 2 years.Conclusion Taking integrated intervention measures with heartcarc network was significant for hygiene.

Objective To observe the curative effect of pricking bloodletting combined with acupuncture on patients with piriformis injury syndrome.Methods A prospective randomized controlled clinical trial was conducted, 84 patients with piriformis injury syndrome admitted to the Department of Acupuncture and Massage in the First Affiliated Hospital of Xinjiang Medical University from May 2011 to May 2014 were enrolled, and they were divided into observation group and control group by random number table, each group being 42 cases. The patients in the observation group were treated with bloodletting once a week (after bloodletting no other treatment was given), and then once a day of acupuncture was performed for 4 days in a week, completing one therapeutic course; while in the control group, the patient was treated with routine acupuncture once a day for 5 days in a week, finishing one therapeutic course. The interval between two therapeutic courses was 2 days, two courses were carried out, and afterwards the clinical therapeutic effects were evaluated in the two groups. The changes of visual analogue scale (VAS) of two groups before and after treatment and the therapeutic effects were observed.Results The VAS score of the two groups after treatment was lower than that before treatment, and the degree of decrease of VAS score in the observation group was significantly lower than that of thecontrol group (1.50±1.35 vs. 2.69±1.68,P < 0.01); the total effective rate in the observation group was obviously higher than that in the control group [100.00% (42/42) vs. 69.05% (29/42),P < 0.01].Conclusion Pricking bloodletting combined with acupuncture therapy for treatment of piriformis injury syndrome can shorten the course of treatment.

Objective To compare the clinical efficacy of through moxibustion combined with electric acupuncture versus electric acupuncture alone for treatment of patients with cervical spondylosis radiculopathy (CSR), so as to provide a basis for optimizing clinical treatment.Methods A prospective study was conducted. Sixty patients with CSR treated in the clinic of Acupuncture Department as outpatients or admitted into the department of the First Affiliated Hospital of Xinjiang Medical University as inpatients were enrolled, and according to the random number table, they were divided into through moxibustion combined with electric acupuncture treatment group (observation group) and conventional electric acupuncture treatment group (control group), 30 cases in each group. The main acupoints of the observation group were as follows: Fengchi, Dazhui, Tianzhu, Houxi, Cervical Jiaji points, Shenshu, Mingmen, in cases with significant neck and shoulder pain, supporting points were added such as Jianjing, Tianzong, in cases with severe arm and finger numbness and pain, Quchi, Hegu, Waiguan, and in cases with marked dizziness and headache, Baihui, Temple acupoints were added. The needle 0.30 mm × 40 mm in size was chosen to be applied, at bilateral neck Jiaji points, direct subcutaneous needling (piercing) was made, at Dazhui, the piercing of needle for 1 to 1.5 inches was performed (body inch), at Mingmen, reinforcing method was used for piercing, at Shenshu, 0.30 mm × 50 mm needle was chosen for piercing with reinforcing method, and at the rest acupoints, conventional neutral supplementation and drainage method of acupuncture was applied. After obtaining qi from needling, electric needle device was added, the current strength used for individual patient depended on his or her degree of tolerance, the needle retention being 20 minutes, and during the above process, at Dazhui and a set of neck Jiaji points, moxibustion with moxa was applied. The acupoints used in the control group were the same as those in the observation group except moxibustion, other procedures were similar to those in the observation group. In both groups, the therapy was taken once a day for consecutive 5 days in a week, constituting one therapeutic course, and after two courses, the changes of clinical symptom scores and clinical efficacies were observed.Results After treatment, the clinical symptom scores in two groups were significantly higher than those before treatment (control group: 15.68±2.01 vs. 7.10±1.87, observation group:18.13±1.69 vs. 7.92±2.14), and the degree of improvement in the observation group was more significant (P < 0.01). After treatment, the total effective rate in observation group was significantly higher than that in the control group [96.67% (29/30) vs. 83.33% (25/30),P < 0.05].Conclusion The clinical therapeutic effect of through moxibustion combined with electric acupuncture for treatment of patients with CSR is better than that of electric acupuncture alone, so there is certain feasibility.

Objective To analyze the epidemic characteristics of mushroom poisoning incident in order to find the regularity of outbreak and provide the fundamental guidelines of prophylaxis,control,diagnosis and treatment.Methods According to the reported information from the Management Information System of Public Health Emergency in China mainland,the area-time distribution of mushroom poisoning incidents from 2004 to 2014 was analyzed,and the descriptive analysis of mushrooms poisoning incidents including causes,places,occupation of victims and incidents identification were made from 2010 to 2014.Results In China (excluding Hong Kong,Macao and Taiwan),the top five provinces of mushroom poisoning incidents were Yunnan,Guizhou,Sichuan,Guangxi and Hunan.The epidemic peak was reached in summer-autumn season.The major and significant incidents accounted for 76.56％ of overall mushroom poisoning incidents,and the fatality rate of 3 701 patients accounted for 21.24％ (786 deaths).The causes were mistaking poisonous mushrooms as edible mushrooms or purchasing poisonous mushrooms in the market by accident.About 87.50％ incidents happened at home.Farmers,workers,children and students were easily subjected to mushroom poisoning because of their large range of activities,strong curiosity and lacking related ability for distinguishing edible mushroom from poisonous mushrooms.No identification was done in 200 mushroom poisoning incidents from 2010 to 2014,which accounted for 92.59％ of mushroom poisoning incidents in the corresponding period.Standard species identification was carried out only in two poisonous mushroom incidents.Conclusions Mushroom poisoning incident was one of the most important causes of death in per-oral poisoning incidents.It should to cope with surveillance and meticulous management during high prevalence season and in high-risk provinces.At the same time,it should be strengthened to train doctors and health professionals with the knowledge of identification of mushroom poisoning in key areas as well as to develop the health promotion of mushrooms poisoning prevention.

Objective To observe the clinical efficacy of acupuncture plus He-Ne laser irradiation in treating cervical spondylotic radiculopathy (CSR) and to provide evidence for optimizing the treatment protocol. Method Totally 120 CSR patients were randomized into group A, group B, and group C, 40 in each group. Group A was intervened by acupuncture plus He-Ne laser irradiation, group B was by acupuncture alone, and group C was by the laser irradiation alone. The CSR rating scale was adopted for observation before and after intervention, and the therapeutic efficacy was also evaluated.Result The CSR rating score was changed significantly in all of the three groups after intervention (P<0.05). The change of CSR rating score in group A was significantly different from that in both group B and C (P<0.05). The total effective rate was 97.5% in group A, versus 92.5% in group C and 80.0% in group C, and the total effective rate of group A was significantly different from that in both group B and C (P<0.05).Conclusion Acupuncture plus He-Ne laser irradiation is an effective method in treating CSR.