Stathmin is a novel member of microtubule-destabilizing proteins that play a critical role in the regulation of the dynamic equilibrium of microtubules during different phases of the cell cycle.The overexpression of stathmin was found in different type of cancer.Inhibition of stathmin expression in malignant cells may interfere with their orderly progression through the cell cycle.Overexpression of stathmin can affect the action of antimicrotuble drugs by markedly decreasing binding of paclitaxel,and increasing binding of Vinca alkaloids.In addition,stathmin provides an attractive molecular target for cancer therapy.It may be possible to combine adenovirus-mediated anti-stathmin ribozyme therapy with a chemotherapeutic agent such as taxol to obtain a more potent antiproliferative and antitumor effect.

Objective To investigate the role of NFIC on the stimulation effects of cAMP-induced differentiation of stem cells from the apical papilla ( SCAPs) in vitro. Methods SCAPs isolated from dental papilla of human imma-ture third molars were cultured by enzyme digestion. SCAPs were transfected with lentivirus that overexpressed NF-IC gene ( ov-NFIC) or an empty vector ( LV-empty) and co-treatment with Forskolin. Mineralized nodule formation of each group was measured by alizarin red staining. Quantitative real-time reverse-transcription polymerase chain reaction was performed to test the expressions of RUNX2,ALP,OCN mRNA. Results Forskolin increased the ex-pression of Runx2, ALP, OCN mRNA as well as matrix mineralization in SCAPs, and the stimulation effects of For-skolin were enhanced by overexpressing NFIC gene. Conclusion The results indicate that NFIC can promote cAMP-induced differentiation of SCAPs.

The tumor microenvironment is closely related to the occurrence and development of tumor. Hypoxia is considered to be one of the most important factors in tumor microenvironment.Formation of hypoxic microenvironment can be found in most of malignant tumors,which can inhibit the anti-tumor immune response. Recent studies have indicated that immunosuppressive cells,tumor stem cells and circulating tumor cells in hypoxic tumor microenvironment can mediate immune suppression and immune tolerance,and then promote development of tumor.The new immune therapy will focus on normalizing tumor vasculature,reconstructing the tumor microenvironment,avoiding immune suppression and averting tumor immune tolerance.

Adolescent ; Blood Vessels ; pathology ; Female ; Femur Head ; blood supply ; pathology ; Femur Head Necrosis ; pathology ; Humans ; Male

Objective To evaluate therapeutic effect of zoledronic acid combined with chemotherapy in patients with cancer and bone metastases. Methods 60 patients with cancer and skeletal me-tastases were devided randomly into tow groups, 30 patients received zoledronic acid combined with chemotherapy, 30 patients only received chemotherapy. Cheotherapeutic program of tow groups were same. Results Response rate of bone pain relief was 83. 3% in zoledronic acid combined with chemotherapeutic group and 56.7% in chemotherapy alone group. Response rate of skeletal metastases was 53. 3% in zoledronic acid combined with chemotherapy group and 20. 0% in chemotherapy alone group. Response rate of movement capacity improvement was 73. 3% in zoledronic acid combined with chemotherapeutic group and 30. 0% in chemotherapy alone group. The therapeutic effect of zoledronic acid combined with chemotherapy group was better than that of chemotherapy alone group( P

Objective To explore whether apply selective perineal skin preparation or not is feasible for patients with coronary heart disease (CHD) before percutaneous coronary intervention (PCI).Methods Divided patients with CHD before PCI into the experimental group (801 cases) and the control group(798 cases) according to the hospital number.Selective perineal skin preparation was used in the experimental group,while the right forearm skin preparation was used in all the patients in the control group.The rate of skin preparation and the care hours in the two groups were compared.Results The care hours of skin preparation in the experimental group was (5.58±0.30) h,which was significant shorter than that in the control group,(66.50±1.50) h,t=-1127.03,P＜0.05.Conclusions Before PCI,for patients with CHD,applying selective perineal skin preparation is feasible.It can not only reduce care labor,decrease nursing costs,but also relieve nurse-patient psychological pressure,cut back patients' expenses;and without percutaneous entrance infection.

Objective To study the effect of astragaloside on proliferation and apoptosis in human keloid fibroblasts.Methods The human keloid fibroblast ceils were treated with different concentration of astragaloside(10、20、40 ng/mL).Cell proliferation was detected by MTT,the gene expreesion levels and protein levels of apoptosis-related proteins,survivin,p53 and Bcl-2.were determined by real-time PCR and Western blot,respectively.Results Comparecl with control group(treated with 0 ng/mL astragaloside),the absorbance values (A490 nm) of each concentration group were significantly reduced,which suggest that the proliferation of all keloid fibroblast were markably inhibited in a dose-dependent way (P＜0.05).The gene expreesion levels and protein levels of apoptosis-related proteins,survivin、Bcl-2 were largely suppressed and P53 werelargely promoted in a dose-dependent.Conclusion The keloid fibroblasts cells proliferation and apoptosis could be regulated by astragaloside.

Objective To investigate the effects of p38 mitogen-activated protein kinase (MAPK) short hair RNA (shRNA) delivered by lentiviral vectors (pGLV) on cardiac function after myocardial infarction (MI) in aldosterone overload rats and to explore the mechanism.Methods Aldosterone overload rat myocardial infarction model was obtained by ligating the left anterior descending coronary artery.The pGLV-shRNA was constructed,sequenced and injected into rats via tail vein.Rats were divided into 3 groups:pGLV-shRNA group (n=6),pGLV-shRNA-NC group (n=6,contained a nonsense shRNA) and the sham-operation group (n=6).Cardiac function was measured by cardiac ultrasound.Apoptosis was assessed by transferase (TdT)-mediated biotin-16-dUTP nick-end labelling (TUNEL).The p38 MAPK mRNA expression was analyzed by RT-PCR.The protein expressions of p38 MAPK and caspase-3 were detected by Western blot.Results Compared with the sham-operation group,cardiac systolic function was reduced and myocardial apoptosis index was significantly increased [(31.26 ± 4.45) ％ vs.(15.20 ± 2.18) ％,P ＜ 0.01] in pGLV-shRNA-NC group.The mRNA and protien expressions of p38MAPK and caspase 3 protein expression were significantly increased in pGLV-shRNA-NC group (all P＜0.01).Compared with pGLV-shRNA-NC group,cardiac function was improved,myocardial cell apoptosis index was reduced [(22.35±3.59)％ vs.(31.26±4.45)％,P＜0.05],and the mRNA and protien expressions of p38MAPK and caspase 3 protein expression were decreased in pGLV-shRNA group (all P＜0.05).Conclusions Cardiac dysfunction is associated with p38MAPK-mediated myocardial apoptosis in aldosterone overload MI rats.pGLV-shRNA may inhibit cardiomyocyte apoptosis and improve postMI cardiac function.

Objective To investigate the characteristics of auditory event-related potentials(AERP) mismatch negativity and P300 in presbycusis patients.Methods Auditory event-related potentials were recorded from 20 presbycusis patients, 20 normal hearing elderly subjects and 20 normal young volunteers(the control group) in the Oddball stimulus paradigm.Mismatch negativity(MMN) was tested under the status of reading.P300 was tested under the status of concentrating.The latencies and amplitudes of 3 groups were compared.Results The latency of MMN in the presbycusis patient group(187.38±29.63ms) was significantly longer than those of the normal hearing older group(160.10±23.21±ms)and the control group(148.22±19.30 ms)(P<0.01).There were statistically significant differences between the normal hearing older group and control group(P<0.05).The amplitude differences were not statistically significant among the 3 groups(P>0.05).The latency of P300 in the presbycusis patient group (369.83±27.09 ms) was significantly longer than those of the normal hearing older group(332.89±25.60 ms)and the control group(318.51±22.32ms)(P<0.01).The amplitude differences were not statistically significant among the 3 groups(P>0.05).Conclusion The presbycusis patients show some central auditory processing dysfunction and cognitive impairment.MMN and P300 might be used as objective methods for evaluating central auditory processing and cognitive function in presbycusis patients.

Objective To detect the Foxp3+ regulatory T cells (TrFoxp3+),Th17,TrFoxp3+/Th17 and related cell factors level in peripheral blood of non-small cell lung cancer(NSCLC) patients,and to explore the relationships about TrFoxp3+,Th17 with occurrence and development of lung cancer and whether there are imbalance of TrFoxp3+/Th17.Methods The proportions of TrFoxp3+,Th17 cells and the level of related cell factors such as TGF-β,IL-17,IL-23 were determined respectively by flow cytometry and ELISA in peripheral blood of 18 healthy people and 26 patients with NSCLC.Results The proportions of TrFoxp3+,Th17 and TrFoxp3+/Th17 in peripheral blood with NSCLC were higher than healthy controls(P ＜0.05).The proportion of Th17 cells from NSCLC patients was positively correlated with that of TrFoxp3+(r =0.81,P＜0.05).Comparing TrFoxp3+/Th17 among different TNM stages in NSCLC patients:TrFoxp3+/Th17 of in Ⅳ stage was obvious higher than that of in Ⅰ-Ⅱ stage,Ⅲ stage(P＜0.05).The level of TGF-β,IL-17,IL-23 was higher in NSCLC patients than that in healthy controls,the ratio of TGF-β in the advanced stage group/healthy controls was higher in the early and middle group/healthy controls (P＜0.05).Conclusion TrFoxp3+,Th17 and TrFoxp3+/Th17 in NSCLC patients were higher than healthy people,moreover TrFoxp3+/Th17 in advanced stage increased significantly.The results may imply that there are certain relationships between the imbalance of TrFoxp3+/Th17 and immunosuppression and occurrence and development of NSCLC as well as the regulation of their cytokines.