Objective:The study focused on detecting the expression of Twist,HIF-1? and HBx in primary liver cancer(PLC) and assessment their relativity.Methods:The expression of Twist,HIF-l? and HBx in 33 cases of PLC and thein paracancerous tissues were examinated with immunohistochemical location technique.Seventeen health control subjects were included in the study. Results:The incidence of Twist,HIF-1?and HBx expression was 93.9%,72.7% and 81.8% in 33cases of PLC,respectively.The positive percentage of Twist in paracancerous tissues was 54.5%.A significant difference was found between the expression of tumor and paracancerous tissures (P

Physiology is one of the most important elementary subjects for medical students.Associated with physiological teaching practice,some experience is shared here to further improve teaching effect.

Child ; Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; surgery

Alveolar Epithelial Cells ; metabolism ; pathology ; Animals ; Apoptosis ; physiology ; Cell Survival ; physiology ; Cells, Cultured ; Epithelial Cells ; cytology ; physiology ; Genes, bcl-2 ; genetics ; Genes, p53 ; genetics ; Oxidative Stress ; genetics ; Rats

Objective To investigate the effect of cosmetic surgery on the facial soft tissue injuries.Methods The clinical data of 52 patients with facial soft tissue injuries from June 2013 to June 2014 were analyzed retrospectively.And plastic surgical techniques were applied to the early treatment of facial soft tissue injuries in patients,according to the size of defect and the degree of deformity of the patients,different plastic surgery treatment was chosen,such as skin flap or skin graft to repair wound surfaces.In this process,one must follow sterile noninvasive principle strictly with emphasis on the technique of plastic surgery such as entire debridement,wound healing application of skin flap and so on.Results Total 49 cases of facial soft tissue injuries were healed by first intention without significant complications,while 4 cases of them had mild scars.Absorbable suture exposition appeared in 3 patients after primary healing.Those sutures were romoved after disinfected and no sequelaes were happened.All patients were recovered with good appearances and functions to the most exent.Conclusions The treatment of facial soft tissue injuries,by applying plastic and aesthetic surgery,could reduce scar and postoperative deformity,and secondary corrective procedures can be avoided in most patients.

Based on the introduction and analysis about PLoS One, and its number of published papers, impact fac-tor, publishing period, manuscript employed, publication fee, auditing, requirement for paper etc. , this paper pointed out break the barriers of traditional publishing model from several aspects, create a new way of open access scholarly pub-lishing, promoted the fast communication of scientific research. As the author of China, can choose more PLoS One pub-lished as our scientific research platform, improve our position in the field of scientific research in the world.

Objective To study the recent effect of ovary dislocation during the operation of cervical squa- mous carcinoma with stageⅠon ovary function.Methods 12 cases with cervical squamous carcinoma of stageⅠwere treated with surgery.During the operation bilateral ovaries with blood vessels were dislocated the abdominal cavity over the umbilical line through postperitoneum and fixed on the postperitoneum.12 cases were hysterec- tomized simply as the control.The change of ovary function was observed in one year after the operation.Results The levels of FSH and E_2 were decreased in the dislocating group(P

Objective To analyze the relationship between WT1 gene polymorphism rs16754 and prognosis of acute myeloid leukemia (AML) by meta-analysis. Methods Studies published in PubMed, CNKI, WanFang and CBM database were searched by using the search terms 'WT1', 'Polymorphism' and 'Leukemia, Myeloid, Acute', respectively. Deadline was December 1, 2015. Results A total of 11 English articles with a total of 2 789 patients were included. Meta-analysis showed that there was no association between polymorphism rs16754 and complete remission of AML patients (RR=1.02, 95 % CI: 0.99~1.06, P=0.20), overall survival(OS) (HR=0.68, 95 % CI: 0.45~1.02, P=0.06), 5-year OS (RR=1.10, 95 % CI:0.90~1.34, P=0.37) or relapse-free survival (RFS) (HR=0.80, 95 % CI: 0.54~1.19, P=0.27). Conclusion There is no correlation between WT1 gene polymorphism rs16754 and the prognosis of AML.

Objective To investigate the relationship between the brachial‐ankle pulse wave velocity (baPWV) with the ca‐rotid arterial intima‐media thickness(IMT) and carotid artery plaque in the patients with type 2 diabetes mellitus(T2DM) and to analyze the related risk factors causing atherosclerosis .Methods A total of 426 patients with T2DM were collected and conduct the records of baPWV ,carotid artery ultrasound ,biochemical index detection andrelated risk factors record .The correlation between baPWV with carotid arterial IMT was analyzed .The patients were divided into the abnormal group and normal group according to the baPWV results .The carotid artery IMT ,carotid artery plaque and related risk factors were compared between the two groups . Results The DM duration ,systolic blood pressure ,diastolic blood pressure ,pulse pressure ,low density lipoprotein cholesterol (LDL‐C) ,number of atherosclerosis risk factors had statistically significant differences between the two groups (P< 0 .05 ,P<0 .01);while the age ,waist circumference ,BMI ,uric acid ,HDL‐C ,TG ,TC ,glycosylated hemoglobin had no statistically significant differences between the two groups(P>0 .05) .The detection rate of plaque by carotid artery ultrasound had no statistically signifi‐cant difference between the two groups(P>0 .05);but the statistically significant difference was found in the number of plaque be‐tween the two groups(P<0 .01) ,the abnormal group was obviously more than the normal group;the normal IMT ratio had statisti‐cally significant difference between the two groups(P<0 .05) ,the normal group was obviously higher than the abnormal group .The IMT value had statistical difference between the two groups(P<0 .01) ,which in the abnormal group was significantly thickened compared with the normal group .The analysis of correlation between baPWV and IMT showed that IMT was positively correlated with baPWV(r=0 .228 ,P<0 .05) .Conclusion T2DM patients with reduced arterial elasticity could complicated with the obvious thickening of carotid arterial IMT and increase of plaque number .Combining baPWV with carotid arterial ultrasound examination can better conduct the arteriosclerosis evaluation .

Diabetic retinopathy is the common complication of diabetes, and the main reason to cause diabetic blindness. The efficacy of Chinese herbal medicine for the treatment of diabetic retinopathy is clear and definite, with the advantages of multiple target points and multiple layers. This article mainly introduced the application of Chinese herbal medicine and the active ingredients in the treatment of diabetic retinopathy.