4.Clinical characteristics and etiology analysis of 223 children undergoing colonoscopy
Shu GONG ; Zhongyue LI ; Xue ZHAN ; Huajian HU ; Yuting WANG ; Jing CHEN
Chinese Journal of Applied Clinical Pediatrics 2016;31(19):1456-1459
Objective To investigate the clinical characteristics and etiology in infants undergoing colonoscopy in order to improve the understandings of lower gastrointestinal tract diseases and their endoscopic manifestations.Methods The clinical and endoscopic data of the infants aged ≤ 3 years old who underwent conoloscopy at the Department of Gastroenterology,Children's Hospital of Chongqing Medical University,from July 2010 to December 2014,were retrospectively analyzed.A total of 223 children were included,148 male and 75 female.The age range was from 26 d to 3 years old(<6 months:41 cases;6-12 months:68 cases;1-3 years old:114 cases).Results A total of 235 colonoscopies were performed under deep sedation by single or double manipulators.Cecal intubation was successful in 192 colonoscopies and terminal ileal intubation was completed in 29 colonoscopies,with a high success rate of 94.0% (221/235 colonoscopies).The main symptoms included hematochezia (124 cases,55.6%),persistent/chronic diarrhea (55 cases,24.7%),hematochezia with chronic diarrhea(22 cases,9.5%),and others(22 cases,9.5%).Of the 223 patients,clear diagnosis were established for 215 children (96.4%),with food protein-induced proctocolitis (FPIPC) (78/223 cases,35.0%),colonic polyps (50/223 cases,22.4%),colitis (29/223 cases,13.0%),antibiotic associated diarrhea (AAD) (19/223 cases,8.5%),FPIPC with AAD (10/223 cases,4.5%).Conclusions Colonoscopy serves as a very important tool for the accurate diagnosis of lower gastrointestinal diseases with hematochezia and/or chronic diarrhea.FPIPC and colonic polyps are the most common causes for hematochezia and/or chronic diarrhea.AAD may be another important cause of chronic diarrhea and bloody stool in infants.Moreover,ghe application of colonoscopy in combination with histopathology can also play an important role in the diagnosis of some rare diseases,such as intestinal tuberculosis,primary intestinal lymphangiectasia,Behcet's disease and primary immunodeficiency disease.
5.Identification of constituents in Suanzaoren tang by LC-Q-TOF-MS and LC-IT-MS.
Yun-Xiang ZHU ; Lu-Lin CHEN ; Jing-Ru GONG ; Shu-Fang WANG
China Journal of Chinese Materia Medica 2014;39(3):457-465
LC-Q-TOF-MS and LC-IT-MS in positive and negative ion mode were applied to simultaneously characterize the constituents in Suanzaoren tang. Analysis was performed on an Agilent Zorbax SB-C18, Rapid Resolution HT column(4.6 mmx 50 mm, 1. 8 micro m) with gradient elution of acetonitrile(A) -aqueous solution containing 0. 05% formic acid(B) at a flow rate of 0. 6 mL min(-1) and the column temperature was 30 degreesC. By comparing MS fragmentation, accurate molecular weight, literature date and standard compounds information, a total of48 compounds were successfully identified or speculated. The origins of these compounds were assigned to the corresponding Chinese medicine. Thirty-one compounds were reported in Suanzaoren tang for the first time. LC-Q-TOF-MS combined with LC-IT-MS is a simple and rapid tool for the identification of constituents of Suanzaoren tang, and the results could provide evidence for the research on quality combined and effective constituents of Suanzaoren tang.
Chromatography, Liquid
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Drugs, Chinese Herbal
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chemistry
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Mass Spectrometry
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Organic Chemicals
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analysis
6.Efficacy of Altemeier operation combined with autogenous anal sphincter constriction in 40 patients with degree II and III rectal prolapse and anal loosening.
Qiao Qiong DAI ; Qin Shu SHAO ; Meng Ling JIANG ; Bing Chen CHEN ; Wen Jing GONG
Chinese Journal of Gastrointestinal Surgery 2021;24(12):1116-1118
7.Proteomics analysis on stressed myocardium injury-related proteins.
Jing-bo GONG ; Shu-qing WU ; Ling-jia QIAN
Chinese Journal of Applied Physiology 2005;21(2):171-174
AIMTo probe the related proteins to stress-induced myocardium injury.
METHODSAfter establishment of a myocardium injury model induced by restraint stress in rats, myocardium proteins of restraint stress-treated and untreated rats were extracted, and the two-dimensional polyacrylamide gel electrophoresis (2-DE) maps of the extracted proteins were established by using the immobilized pH gradient (IPG) and SDS-PAGE two-dimensional electrophoresis respectively. The alterative protein spots were analyzed by Image Master 3.01 software and identified with assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and database searching.
RESULTSProteomics analysis showed that there were 10 proteins were significantly influenced by restraint stress in rat myocardium. After stress, proteins, including cardiac myosin heavy chain, dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex, similar to dihydrolipoamide S-succinyltransferase, mitochondrial aldehyde dehydrogenase, H (+)-transporting ATP synthase, albumin, myosin heavy chain and apolipoprotein A-I precursor showed increased expression. Mitochondrial aconitase and uncoupling protein UCP-3 showed decreased expression.
CONCLUSIONThese differential expressive proteins might be involved in stress-induced injury to myocardium.
Animals ; Disease Models, Animal ; Heat-Shock Proteins ; metabolism ; Male ; Myocardium ; metabolism ; Proteomics ; Rats ; Rats, Wistar ; Restraint, Physical ; Stress, Physiological
8.Application of PDCA cycle in the hospital management of multidrug-resistant organisms
Chinese Journal of Infection Control 2018;17(3):256-259
Objective To analyze the change in isolation rates of multidrug-resistant organisms (MDROs) before and after adopting plan-do-check-act (PDCA) cycle method for management of MDROs. Methods Bacterial culture specimen submission and isolation of MDROs in a tertiary first-class hospital before the implementation of PDCA cycle (January 2013-December 2014) and after implementation of PDCA cycle (January 2015-December 2016) were collected and analyzed. Results A total of 14 889 specimens were sent for detection before the implementation of PDCA cycle, 6 345 strains were isolated, 650 of which were MDROs, isolation rate of MDROs was 10. 24%; after the implementation of PDCA cycle, 17 856 specimens were sent for detection, 7 568 strains were isolated, 476 were MDROs, isolation rate of MDROs was 6.29%; difference in MDRO detection rate before and after the implementation of PDCA was statistically significant (X2=72.567, P<0.001). After Cochran-Armitage trend test, the isolation rates of MDROs in 2013-2016 showed a decreased trend (Z= - 7.8856). The amount and cost of hand hygiene products have increased. Conclusion By carrying out PDCA cycle for MDROs management, the isolation rate of MDROs in hospital is reduced. PDCA cycle management method can effectively promote the continuous quality improvement of hospital MDROs management.
9.Effect of homocysteine on injury of cardiomyocytes and its signal transduction mechanism.
Shu-Qing WU ; Jing-Bo GONG ; Liang-En CHEN ; Ling-Jia QIAN
Chinese Journal of Applied Physiology 2003;19(1):25-29
AIMTo observe the injured effect of homocysteine (HCY) on cardiomyocytes and investigate its signal transduction mechanism as well as the key regulatory link.
METHODSCardiomyocytes were isolated from neonatal Wistar rats. After incubation with HCY, the survival rate of cardiomyocytes was determined by trypan blue stained assay, while the apoptosis rate was measured by TUNEL and FCM. Western blot and EMSA were used to tested ERK2 protein phosphorylation and NF-kappaB active expression in cardiomyocytes, respectively.
RESULTSThe survival rate of cardiomyocytes treated with HCY was reduced significantly in dose- and time- dependent manner. It was found that 10(-3) mol/L HCY could increase the apoptosis rate of cardiomyocytes to the peak (7.65%) at 4 h stress. Several HCY levels revealed the strong inhibitory effect on ERK2 protein phosphorylation, especially, 10(-3) mol/L HCY decreased the level of active ERK2 expression to 3.04% of control at 4 h (P < 0.01). NF-kappaB activation was also inhibited significantly by several HCY level for different time in cardiomyocytes.
CONCLUSIONHCY plays an important role in injury of cardiomyocytes and apoptosis is a form of HCY-induced injury to cardiomyocytes. HCY can block ERK2 protein phosphorylation and NF-kappaB activation, which contribute to the injury of cardiomyocytes.
Animals ; Apoptosis ; drug effects ; Cells, Cultured ; Homocysteine ; pharmacology ; Myocytes, Cardiac ; drug effects ; metabolism ; NF-kappa B ; metabolism ; Rats ; Rats, Wistar ; Signal Transduction
10.The modulation of pilose antler extract (PAE) on the proliferation of rat osteogenic cells UMR-106.
Xiao-chao CHEN ; Li-jing KE ; Gong-rui CHEN ; Shu-tao LIU ; Yu-shu HUO ; Ping-fan RAO
China Journal of Chinese Materia Medica 2004;29(1):74-77
OBJECTIVETo investigate the modulation of pilose antler extract (PAE) on rat osteogenic cells UMR-106 in vitro.
METHODComponent P2 of PAE was isolated by Sephacryl S-200HR gel filtration chromatography. The proliferative effects of P2 and other components isolated by Sephacryl S-200HR on UMR-106 cells were investigated by MTT assay.
RESULTThe P2 could significantly increase the proliferation rate of osteogenic cells. When the protein concentration of P2 was between 0.972 mg x L(-1) and 97.2 mg x L(-1), it could inhibit the proliferation of UMR-106 cells. But while the concentration was equal to or greater than 97.2 mg x L(-1), the P2 could increase the proliferation rate of cells, especially 477.92% at 9.72 g x L(-1), which was approximated to 499.62% of PAE. The molecular weight of the P2 was about 59 kDa determined by SDS-PAGE. On the other hand, inhibition was also observed in the sample of the P3, P4 and P5.
CONCLUSIONThose regulative factors in PAE which have different molecular weight can affect the proliferation of UMR-106 cells two-wayly. And this adjustment also relies on the dose of those factors. This finding may help us to understand the possible mechanism of Chinese traditional medicine from animal materials.
Animals ; Antlers ; chemistry ; Bone Neoplasms ; pathology ; Cell Line, Tumor ; Cell Proliferation ; drug effects ; Deer ; Materia Medica ; isolation & purification ; pharmacology ; Osteosarcoma ; pathology ; Rats ; Tissue Extracts ; isolation & purification ; pharmacology