This review was made to compare the multimedia teaching results with traditional teaching results in medical universities or colleges.Two kinds of opposite viewpoints appeared:the multimedia teaching results was better or worse than the latter.

Objective To provide reference for the clinical application of preoperative autologous blood donation(PABD).Meth-ods 34 patients met the conditions of PABD,and underwent PABD were enrolled in the study.Five laboratory indicators include Hemoglobin(Hb),RBC,WBC,PLT)and hematocrit(HCT),and prepared blood volume before surgery,intraoperative and postop-erative reinfusion volume were recorded before blood collection and after reinfusion.Results In the patients who only underwent PABD(A group),compared between before blood collection and after blood transfusion(intraoperative or postoperative 24 h),all the five Hematology Index were statistically different(P <0.05).The RBC,Hb,HCT,PLT were decreased(P <0.05),while WBC increased(P <0.05).In patients underwent both PABD and allogeneic blood transfusion blood(group B),the five laboratory indica-tors showed a similar pattern:RBC,Hb,HCT,PLT decreased (P <0.05),while WBC increased(P <0.05).In the comparison be-tween the two groups,the five indicators before blood collection and after reinfusion were not statistically different(P >0.05).Con-clusion The effective of PABD application in surgical patients undergoing elective operation is good,which won′t cause harm to the patient′s body(reflected in the hematology indicators).However,in some cases preoperative assessment of blood loss is not accurate enough,result in the amount of blood collected before operaton not meeting the needs,which might impact the effect PABD.

Mitochondria DNA was isolated from darkgrown shoots of Xi Feng A according to the procedure reported previously. Mitochondria DNA was digested with EcoR1 completely, and ligated with pUC19 vector DNA which was digested with EcoR1. The ligated DNA was transformed into E. coli JM101. The transformants were selected on LB medium which containing ampicillin 50ug/ml. All of the bacterial clones were hybridized with 32P-labelled maize 26S rRNA gene, seven positive clones were obtained. The plasmid DNAs were isolated from the clones and digested with EcoR1 and southern transferred to NC paper. The membrance was hybridized with maize 26S rRNA probe. One clone which a shortest hybridization band (1.3kb) was named pXMT1.

OBJECTIVE:To screen the optimal technology of dog testes and penis processed with talcum powder,and to pro-vide reference for the formulation of quality control standard. METHODS:Taking the comprehensive score of crushing rate and the content of alcohol soluble extract as evaluation indexes,L9(34) orthogonal test was used to optimize processing temperature,pro-cessing time and the amount of talcum powder;validation test was conducted. According to related method in Chinese Pharmaco-poeia(2015 edition),the contents of moisture,ash,nitrogen and alcohol soluble extract were determined,and automatic amino ac-id analyzer was used to determine the content of amino acids in samples. RESULTS:The optimal processing technology were that 100 kg dog testes and penis should add into 40 kg talcum powder;the processing temperature was 350-380 ℃;processing lasted for 4 min. Results of verification test showed that the average crushing rate and the content of alcohol soluble extract were 80.2%(RSD=0.95%,n=3)and 15.7%(RSD=2.30%,n=3). In processed dog testes and penis,the contents of moisture,ash,nitro-gen and alcohol soluble extract were 5.7%,18.7%,8.3%,15.7%,respectively. Besides,there still were 16 kinds of amino ac-ids,and their total content was 42.44%. CONCLUSIONS:The optimized talcum powder processing technology of dog testes and penis is stable and practical. The content of moisture,ash,nitrogen,alcohol soluble extract and amino acid can be used as impor-tant quality standard control indexes for processed dog testes and penis.

10.3969/j.issn.1007-3969.2013.04.009

ObjectiveTo study the nursing characteristics in surrounding period of operation for treating carotid artery stenosis with stenting angioplasty. MethodsThe nursing procedure for 78 cases with carotid artery stenosis were reviewed to form the duties and items of nursing, including cooperation between doctors and nurses, mentality nursing, instruction of convalescence in the pre-operation, during operation and post-operation. ResultsAll the cases has successfully finished their operative procedure and none serious complication has been found. ConclusionThe nursing play a important role in the stenting angioplasty of carotid artery.

Objective To explore effect of physic temperature dropping by ice blanket on incidence of pressure ulcer of stroke patients in ICU, and normalize the basic nursing procedure during temperature reduc-tion.Methods 230 fever patients were randomly divided into the group A(control temperature with ice blan-ket,108 cases)and the group B (control temperature with routine measure,122 cases).Thc incidence of pressure ulcer and surface temperature of pressed position in the two groups were compared.Results The incidence of pressure ulcer and surface temperature of pressed position in group A was lower than those of group B.Conclusions The incidence of pressure ulcer can be reduced by using ice blanket for physic temperature dropping.The pressure of pressed position were descended by alternative prostration and lateral position.

OBJECTIVETo explore the role of Compound Danshen Injection (CDI) in regulating the expression of aquaporin 3 (AQP3) in human amnion epithelium cells (hAECs), and to study the relation between c-Jun N-terminal kinase (JNK) signal pathway and AQP3.

METHODShAECs were isolated and primarily cultured from term pregnancy with normal amniotic fluid volume and from term pregnancy with oligohydramnios, and then hAECs were further divided into four groups, i.e., the blank control group (A), the SP600125 group (B), the CDI group (C), and the SP600125 +CDI group (D). The cell viability was measured by cell counting kit-8 assay (CCK-8). The expression of total JNK, phosphorylated JNK, and AQP3 were determined by Western blot.

RESULTS(1) In hAECs with normal AFV or with oligohydramnios: There was no statistical difference in the cell viability or the expression of total JNK among the 4 groups (P > 0.05). But there was statistical difference in the expression of p-JNK (P < 0.05). Compared with A group, the expression of p-JNK was obviously down-regulated in B group, but obviously up-regulated in C group (P < 0.05). The expression of p-JNK was significantly lower in D group than in C group, but higher than that in A group or B group (P < 0.05).The AQP3 expression in the hAECs with normal amniotic fluid volume of C group and D group were higher than that in the A group (P < 0.05). However, there was no statistical difference in the AQP3 expression between C group and D group (P > 0.05). In hAECs with oligohydramnios, the expression of AQP3 obviously decreased in B group, but up-regulated in C group (both P < 0.05). The expression of AQP3 was lower in D group than in C group, but higher than in B group (P < 0.05).

CONCLUSIONCDI could regulate the AQP3 expression in hAECs with oligohydramnios via activating the JNK signal pathway.

Amnion ; cytology ; drug effects ; Aquaporin 3 ; metabolism ; Cells, Cultured ; Drugs, Chinese Herbal ; pharmacology ; Epithelial Cells ; drug effects ; metabolism ; Female ; Humans ; JNK Mitogen-Activated Protein Kinases ; metabolism ; MAP Kinase Signaling System ; physiology

Objective:To study the effects of the fastigial nucleus electrostimulation on the ability of learning and memory and the Apoptosis of the neurons in the hippocampal district in ratymodel of Alzheimer's D isease induced by?-amyloid(A?).Methods:Forty rats were randomly divided into:A?1-40 micro-injection groups(AD),sham-operation roup(SC),A?1-40 micro-injection and fastigial nucleus stimulation group(FNS),a A?1-40 micro-injection and corpus dentatum stimulation group(DNS).The effect of fastigial nucleus electrostimulation on learning and memory retention disorder in the model rats was studied by the test of Morris water maze;the brain protection of fastigial nucleus electrostimulation in model rats was observed through pathomorphology changes;the Apoptosis of the neurons in the hippocampal CA1 district was detected by Terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling method.Results:The escape latency of the place navigation in the AD grou,FNS group and DNS group were singnificiantly longer than that in the SC group(P

Animals ; Aorta ; drug effects ; physiology ; Blood Pressure ; drug effects ; Cadmium ; toxicity ; Female ; In Vitro Techniques ; Male ; Muscle, Smooth, Vascular ; drug effects ; physiology ; Rabbits ; Vasoconstriction ; drug effects