Objective: To investigate the relationship between circulating endothelial cell(CEC)(numbers),blood lipid levels and multiple organ dysfunction syndrome(MODS) and their clinical(significance).Methods: CECs were separated by isopyknic centrifugation method and blood lipid was(determined) in(35 MODS) patients and 20 healthy controls.CEC numbers and blood lipid were compared in both groups.(Acute) physiology and chronic health evaluation Ⅱ(APACHE Ⅱ) score was recorded to evaluate the severity of the MODS patients.The relationships were analyzed between APACHEⅡ score and CEC numbers,(between) APACHEⅡ and blood lipid levels and between CEC numbers and blood lipid levels.Results: CEC numbers in MODS patients were significantly higher than those in healthy controls(P

Objective To discussing the methods used in creating non linear developable biomechanical models of skeletal muscle.Methods One of the basic tools of the analytical approach in analytical mechanics, the principle of virtual work for deformable systems, was used to create the model. By means of method of Lagrange multipliers, the problems of constraints, such as the incompressibility of skeletal muscle, were effectively tackled. Results and Conclusion The biomechanical models of skeletal muscle has been developed. The program of this model can be used to obtain nodal displacement coordinates of given nodal coordinates and parameters.

Objective To study the effects of valsartan and triptergium glycosides on the abnormal expression of connective tissue growth factor(CTGF) in rats with diabetic nephropathy. Methods Sixty male SD rats were randomly divided into normal control group,diabetic nephropathy group(DN group),diabetic nephropathy treated with valsartan group(VAL group) and diabetic nephropathy treated with triptergium glycosides group(TG group).Diabetic nephropathy model was established by intraperitoneal injection of streptozotocin(STZ) in DN group,VAL group and TG group.The rats were sacrificed after 2,4,8 and 12 weeks.The body weight,blood glucose,renal function and urinary albumin excretion rate(UAER) were measured,and the expression of CTGFmRNA in kidney was detected by RT-PCR. Results Compared with control group,the levels of blood glucose,blood urea nitrogen,serum creatinine and UAER were significantly increased in DN group(P

Objective To study the changes of the number of circulating endothelial cells (CEC) and blood lipid level in critically ill patients and their clinical significance. Methods The CECs were separated by using isopyknic centrifugation method and blood lipid level was determined in 35 critically ill patients and 20 healthy controls. CEC number and blood lipid level were compared between the two groups. And the correlation of CEC number and blood lipid level was analyzed. Clinical data from the critically ill patients was evaluated with acute physiology and chronic health evaluation Ⅱ; the correlation analysis of APACHEⅡscore, CEC number and blood lipid level was made. Results CEC number in the critically ill patients was significantly higher than that in healthy controls(P

Objective To investigate in vitro anti-hydatid efficacy on Echinococcus granulosus protoscolex(EgPSC) by using albendazole sulfoxide(ASOX) and its two enantiomeric antipodes, L-ASOX and D-ASOX.Methods Eg protoscoleces were divided into eight groups and cultured in the DMEM culture media under two concentrations(50 ?g/ml and 100 ?g/ml) of ASOX, L-ASOX and D-ASOX respectively.The appropriate controls included(i) a culture containing an equal amount of DMSO and(ii) a culture medium alone.The mortality of EgPSC in each group was daily counted until 100% EgPSC death in some groups.Results Significant difference of EgPSC mortality was found among the three drugs with various concentrations compared to control group(P0.05), but between ASOX group and L-ASOX group(P

Objective To investigate the effects of rosiglitazone,an agonist of proliferators activated receptor?(PPAR?),on expression of nuclear factor-kappa B(NF-?B)in peripheral blood mononuclear cells(PBMC)of multiple organ dysfunction syndrome(MODS) rats.Methods Forty male SD rats were randomly divided into four groups(n = 6 per group): vehicle control group,endotoxin(LPS) group,rosiglitazone(ROSI) pretreatment group,and PPAR-?antagonist GW9662 pretreatment group.Blood was taken 4 hours after operation,and mononuclear cells were separated.The expression of NF-?B p65in PBMC was detected by immunocytochemical method and image analysis was carried out.Results The expression of NF-?B p65 was low in vehicle control group.In LPS group,the expression of NF-?B p65 was significantly higher than that in vehicle control group(P0.05).Conclusion Rosiglitazone protected the MODS rats by inhibiting NF-?B activation.

BACKGROUND: The expression and significance of transforming growth factor-alpha (TGF-α) and epidermal growth factor receptor (EGF-R) in different diseases are different.OBJ ECTTVE: To investigate the expression of TGF-α and EGF-R in human liver cirrhosis tissues.DESIGN : Non-randomized controlled trial.SETTING: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University of Chinese PLA.MATERIALS: This trial was carried out in the Department of Pathology, Xijing Hospital, Fourth Military Medical University of Chinese PLA during March 2003 to May 2004. Sixty-three samples of human liver cirrhosis tissue were obtained from Department of General Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA (Informed consents were obtained). Five human normal liver tissues were obtained form autopsy in Department of Pathology, Fourth Military Medical University of Chinese PLA (patients with liver diseases were excluded).METHODS: Sixty-three samples of human liver cirrhosis specimens and five human normal liver tissues were studied with immunohistochemical method and in situ hybridization technique. The positive rates of TGF-α and EGF-R were calculated and managed statistically.MAIN OUTCOME MEASURE: Detection of the expressions of TGF-α and EGF-R in liver cirrhosis tissues were detected with immunohistochemical method and in situ hybridization technique.RESULTS: The expression rate of TGF-α and EGF-R in 63 samples of human liver cirrhosis tissues was 84% (53/63)and 52% (33/63) respectively. These positive granules were brown and most of them were in cytoplasm of hepatocytes.There was significantly positive correlation between TGF-α and EGF-R (r =0.32,P ＜ 0.05). In five normal liver tissues,TGF-α and EGF-R were not detectable in hepatocytes and bile ducts. In situ hybridization showed the levels of TGF-αmRNA and EGF-R mRNA were higher (86%, 54%) than those of immunohistochemistry. There was no significant difference between two detective methods (P＞0.05).CONCLUSrON: Liver cirrhosis might be under the autocrine regulation of TGF-α/EGF-R. Increasing expression of TGF-αand EGF-R might be one of important factors in liver cirrhosis pathogenesis.

Objective To study the impedance detected by the whole blood electrical impedance aggregometry in evaluating the effect of antiplatelet therapy.Methods The imprecision of the two different assays(the whole blood electrical impedance aggregometry and the plasma light transmission aggregometry) was compared.Thirty healthy volunteers and 60 patients with coronary artery disease under antiplatelet treatment were enrolled in the present study,whose anti-aggregated blood were collected for the measurement of electrical impedance in the presence of ADP,arachidonic acid and collagen,respectively.Finally,we analysed the agreement between the results of the two different assays which were used to measured the antiplatelet effect of aspirin and clopidgrel.Results The imprecision analysis showed the CV,(the whole blood electrical impedance aggregometry was 4.8%,superior to the plasma light transmission aggregometry (CV = 7.7%).In healthy control group,the mean impedances were(9.6?3.4)?、(10.4?2.8)? and (13.5?2.2)? in the presence of ADP,arachidonic acid and collagen as in the patients taking aspirin 100 mg/day,the 95% patients' results were 0 ?.Furthermore,the mean impedance of patients taking aspirin 100 mg/day and/or clopidogrel 75 mg/day was significantly lower than that of healthy volunteers(ADP as agonist,t = 2.391,P

Objective: To study the serum sex hormone level and outer behavior changes of the rats that had gestated one fetus and been used to imitate the Gan qi adverseness of PMS. Methods: Selecting the appropriate time to stimulate the rats by impulse current to make the model and mensurating serum sex hormone level by radioactive kit. Results: The PRL level of the rats that were in the anaphase of their estrus raised evidently and the levle of E 2 and P reduced evidently.Conclusion: The model approximately reflected the clinical feature and pathological changes of the liver qi invasion of PMS. The model was made successfully.

Objective To investigate the clinical value of color Doppler flow imaging(CDFI) in the diagnosis and clinical follow-up of retinopathy of prematurity(ROP).Methods From March 2008 to February 2009,98 eyes of 49 premature infants(gestational age≤37 weeks,or birth weight≤2 000 g) underwent ROP screening by RetCam digital retinal camera and indirect ophthalmoscopy in the fourth to sixth postnatal week.At the same time,they were also examined by 8-14 MHz CDFI.Results Determined by RetCam digital retinal camera and indirect ophthalmoscopy,ROP was found in 41 eyes,including 8 eyes at stage Ⅰ,19 eyes at stage Ⅱ,6 eyes at stage Ⅲ,8 eyes at scarring stage and no eye at stage Ⅳ andⅤ.Compared with ophthalmologic examination,the specificity of CDFI for ROP diagnosis was 85.5%,and the sensitivity was 76.7%.Detected by CDFI,typical changes were not found in the infants with ROP at stage Ⅰ.In the infants with ROP at stage Ⅱ and Ⅲ,abundant blood flow signals were found in the central retinal artery(CRA).Increased amounts of the small vessels and irregular circuitousness were also observed.There was no obvious change in the systolic velocity(VS) of CRA.The diastolic velocity(Vd) was decreased and the resistance index(RI) was increased.A strong zonal echo protruded into the vitreum.Small blood flow signals which came from CRA by tracing toward their distal end were found in the same position using CDFI.Conclusions Detected by CDFI,the sonographic features of infants with ROP at stage Ⅰ are not typical,but those of infants with ROP at stage Ⅱ and Ⅲ are specific.CDFI is valuable in clinical ROP screening of high risk group.