Objective: To investigate the relationship between circulating endothelial cell(CEC)(numbers),blood lipid levels and multiple organ dysfunction syndrome(MODS) and their clinical(significance).Methods: CECs were separated by isopyknic centrifugation method and blood lipid was(determined) in(35 MODS) patients and 20 healthy controls.CEC numbers and blood lipid were compared in both groups.(Acute) physiology and chronic health evaluation Ⅱ(APACHE Ⅱ) score was recorded to evaluate the severity of the MODS patients.The relationships were analyzed between APACHEⅡ score and CEC numbers,(between) APACHEⅡ and blood lipid levels and between CEC numbers and blood lipid levels.Results: CEC numbers in MODS patients were significantly higher than those in healthy controls(P

Objective To discussing the methods used in creating non linear developable biomechanical models of skeletal muscle.Methods One of the basic tools of the analytical approach in analytical mechanics, the principle of virtual work for deformable systems, was used to create the model. By means of method of Lagrange multipliers, the problems of constraints, such as the incompressibility of skeletal muscle, were effectively tackled. Results and Conclusion The biomechanical models of skeletal muscle has been developed. The program of this model can be used to obtain nodal displacement coordinates of given nodal coordinates and parameters.

Objective To study the changes of the number of circulating endothelial cells (CEC) and blood lipid level in critically ill patients and their clinical significance. Methods The CECs were separated by using isopyknic centrifugation method and blood lipid level was determined in 35 critically ill patients and 20 healthy controls. CEC number and blood lipid level were compared between the two groups. And the correlation of CEC number and blood lipid level was analyzed. Clinical data from the critically ill patients was evaluated with acute physiology and chronic health evaluation Ⅱ; the correlation analysis of APACHEⅡscore, CEC number and blood lipid level was made. Results CEC number in the critically ill patients was significantly higher than that in healthy controls(P

Objective To study the effects of valsartan and triptergium glycosides on the abnormal expression of connective tissue growth factor(CTGF) in rats with diabetic nephropathy. Methods Sixty male SD rats were randomly divided into normal control group,diabetic nephropathy group(DN group),diabetic nephropathy treated with valsartan group(VAL group) and diabetic nephropathy treated with triptergium glycosides group(TG group).Diabetic nephropathy model was established by intraperitoneal injection of streptozotocin(STZ) in DN group,VAL group and TG group.The rats were sacrificed after 2,4,8 and 12 weeks.The body weight,blood glucose,renal function and urinary albumin excretion rate(UAER) were measured,and the expression of CTGFmRNA in kidney was detected by RT-PCR. Results Compared with control group,the levels of blood glucose,blood urea nitrogen,serum creatinine and UAER were significantly increased in DN group(P

Objective To investigate in vitro anti-hydatid efficacy on Echinococcus granulosus protoscolex(EgPSC) by using albendazole sulfoxide(ASOX) and its two enantiomeric antipodes, L-ASOX and D-ASOX.Methods Eg protoscoleces were divided into eight groups and cultured in the DMEM culture media under two concentrations(50 ?g/ml and 100 ?g/ml) of ASOX, L-ASOX and D-ASOX respectively.The appropriate controls included(i) a culture containing an equal amount of DMSO and(ii) a culture medium alone.The mortality of EgPSC in each group was daily counted until 100% EgPSC death in some groups.Results Significant difference of EgPSC mortality was found among the three drugs with various concentrations compared to control group(P0.05), but between ASOX group and L-ASOX group(P

Objective To investigate the effects of rosiglitazone,an agonist of proliferators activated receptor?(PPAR?),on expression of nuclear factor-kappa B(NF-?B)in peripheral blood mononuclear cells(PBMC)of multiple organ dysfunction syndrome(MODS) rats.Methods Forty male SD rats were randomly divided into four groups(n = 6 per group): vehicle control group,endotoxin(LPS) group,rosiglitazone(ROSI) pretreatment group,and PPAR-?antagonist GW9662 pretreatment group.Blood was taken 4 hours after operation,and mononuclear cells were separated.The expression of NF-?B p65in PBMC was detected by immunocytochemical method and image analysis was carried out.Results The expression of NF-?B p65 was low in vehicle control group.In LPS group,the expression of NF-?B p65 was significantly higher than that in vehicle control group(P0.05).Conclusion Rosiglitazone protected the MODS rats by inhibiting NF-?B activation.

Objective To upgrade No.1 Military Medical Project from Oracle8i to Oracle10g. Methods Through the tools of Exp/Imp and Toad, the database migration was realized. Results Oracle database was upgraded from Oracle8i to Oracle10g successfully. Conclusion The method is simple,easy to operate and suitable for application.

Objective To describe the application situation, existent problems and insufficiencies of the electronic medical record (EMR) based on XML, and discuss the future development and requirement of EMR. Methods In recent three years, the application practice and current development condition of EMR based on XML were summarized. Results The EMR based on XML was provided considerable convenience and advantage for writing of medical record and the monitor of medical quality. Conclusion The electronic medical records are an important part of client information system (CIS), has become a necessary part of hospital informationization, and its extensive application has a long way to go.

Objective To expound emphatically the application of nuclear medical examination in primary hyperparathyroidism.Methods 22 patients (19 adenomas,3 hyperplasia) with primary hyperparathyroidism confirmed by clinical examination and surgery underwent the detection of parathormone (PTH) by immunoradiometric assay (IRMA) and double phase 99 Tcm-MIBI parathyroid imaging,of whom 15 performed 99 TcmO-4 thyroid imaging and 8 99 Tcm-MDP bone imaging.Results The level of serum PTH in all patients (428.21±256.48) was significantly higher as compared to normal value and in cases with adenomas (781.83±194.56) was markedly elevated.The sensitivity of 99 Tcm-MIBI early and delayed phase imaging in detecting lesions of parathyroid adenomas was 84.2% and 100%,respectively.The sensitivity in detecting lesions of hyperplasia was only 33.3%.With 99 Tcm-O-4 thryoid imaging,the cold nodules were present in 2 and normality in 13.As to bone imaging,the scans were abnormal in 7.Their severities on bone scintigraph were correlated with the duration of the disease.Conclusions The level of serum PTH detected by IRMA and double phase 99 Tcm-MIBI parathyroid imaging are both sensitive methods in qualitative diagnosis and localization of hyperthyroidism.99Tcm-MIBI imaging has limited value for detecting the hyperplasia.The thyroid and bone imaging may well improve the diagnostic sensitivity and accuracy,and reflect the stage and progress of hyperparathyroidism.

Objective To evaluate the effects of sevoflurane postconditioning on mitophagy during myocardial ischemia-reperfusion (I/R) in rats.Methods Forty-two pathogen-free adult male SpragueDawley rats, weighing 250-300 g, were randomly divided into 3 groups (n=14 each) using a random number table: sham operation group (group S) , I/R group and sevoflurane postconditioning group (group SP).Myocardial I/R was induced by 30 min ligation of the left anterior descending branch of the coronary artery followed by 2 h of reperfusion.In group SP, 2.4％ sevoflurane was inhaled for 15 min starting from the onset of reperfusion, while 33％ oxygen was inhaled in group I/R.The rats were sacrificed at the end of reperfusion, and the hearts were removed for measurement of myocardial infarct size (by 1％ 2, 3, 5 triphenyltetrazolium chloride) , expression of LC3 Ⅱ/LC3 Ⅰ , Beclin-1, p62 and Parkin (by Western blot) ,and mitochondrial menbrane potential (by using JC-1 probe) , and for examination of the uhrastructure of cardiomyocytes (with transmission electron microscope).Results Compared with group S, the myocardial infarct size was significantly increased, mitochondrial membrane potential was decreased, the expression of LC3 Ⅱ/LC3 Ⅰ , Beclin-1 and Parkin was up-regulated, and the expression of p62 was down-regulated in group I/R.Compared with group I/R, the myocardial infarct size was significantly decreased, the mitochondrial membrane potential was increased, and the expression of LC3 Ⅱ/LC3 Ⅰ , Beclin-1, p62 and Parkin was down-regulated in group SP.Conclusion Sevoflurane postconditioning can mitigate I/R injury in rats, and inhibition of excessive activation of mitophagy may be involved in the nechanism.