Traditional Chinese medicine is the few field of owing independent intellectual property in China, the implement of intellectual property strategy is important for the long-term development of traditional Chinese medicine enterprises. At present, traditional Chinese medicine enterprises still remain many problems in intellectual property protection, such as weak awareness of intellectual property protection, lack of intellectual property protection professional personnel, inadequate investment in R & D and low innovation level. These problems caused huge losses in practice to traditional Chinese medicine enterprises. After analyzing the status of intellectual property protection of traditional Chinese medicine enterprises, we put up with some concrete measures of intellectual property protection of traditional Chinese medicine considering the reality of these enterprises.

Objective To study the effects of ceftriaxone sodium(Cef) on the seizures and the expression of glutamate transporter (GLT-1) in kainic acid (KA) epilepsy model.Methods Firstly, a chronic spontaneous seizure mouse model was established by unilateral hippocampal injection of KA and monitored by vEEG technique to record seizures.The experimental group received intraperitoneal injection of Cef 200 mg/(kg·d) and the control group received normal saline.Seizure frequency, interictal spike waves and histological phenotypes were recorded to evaluate the function of Cef.Then we use the Western blot to detect the effect of expression for GLT-1.Results Unilateral hippocampal injection of KA 200 ng successfully established the mesial temporal lobe epilepsy model.Cef can reduce the seizures from 2.145 times/day to 1.597 times/day, decreased by 31.2% with a statistical significance(P<0.05).Cef treatment did not significantly enhance the expression of GLT-1.Conclusions Intraperitoneal injection of Cef partially inhibites the seizures of KA model, but the expression of GLT-1 in hippocampus is not enhanced.It is suggested that ceftriaxone may inhibit seizures through other mechanisms.

Objective:Peripherally inserted central catheter(PICC) has been application in clinical for many years,but the training in the knowledge about maintenance wasn't univesal systematically yet.To analyze the existed problems on the clinical peripherally inserted central catheter in washpipe technology and find out countermeasures. Methods: The self-made questionnaire was used to survey a total of 46 nurses in every department and make on-site operation demonstration. Results: Only 12 nurses' operation was completely correct and the correct rate was 26.1percent,which was related to the training,manager's attention,nurses' participate awareness. Conclusion: The training on nurses' operation of red tube needed to be strengthen.

Beta blocker can be categorized by non-selective agents,selective agents and agents with vasodilatory effect.Beta blocker therapy represents a major advance in the treatment of heart failure patients.The mechanisms include up-regulation of beta receptor,reversing remodeling,improving contractile and diastolic function,antiarrhythmia and anti-ischemia.Evidences from randomized controlled clinical trials have supported its use in the treatment of patients with heart failure to decrease mortality and improve life quality.Guidelines with various versions recommend that ?-blockers are indicated for patients with symptomatic or asymptomatic heart failure and a depressed ejection fraction of lower than 40%.There is something to be noticed for rational use of ?-blockers such as timing,dosage,duration and contraindications.

Eukaryotic elongation factor 2 kinase (eEF2K) is well known as a Ca2+/calmodulin (CaM)-dependent kinase. eEF2K catalyzes the phosphorylation of eEF2 and subsequently inactivates eEF2 by impairing its ability to bind to the ribosome, thereby negatively modulates protein synthesis. The high expression of eEF2K has been found recently in several types of malignancies. As participating in the progress of tumor, eEF2K emerges a potential target for future cancer therapy. The relationship between eEF2K and tumor, and the latest progress of eEF2K inhibitors were summarized in this article.
Elongation Factor 2 Kinase ; antagonists & inhibitors ; metabolism ; Humans ; Neoplasms ; metabolism ; Peptide Elongation Factor 2 ; metabolism ; Phosphorylation

Objective:To observe HSP70 expression during experimental tooth movement in rats.Methods:Orthodontic appliance was placed between the maxillary right first molar and maxillary central incisors of 30 adult SD rats.The rats were killed at 1,3,5,7,14 d after orthodontic force application,samples of experimental teeth with periodontal tissue were prepared for immunohistochemical examination of HSP70 expression.Result:Strong expression of HSP 70 in periodontal ligment was observed at 1 and 3 d after orthodontic force application,positive at 5 d and weak positive at 7 and 14 d.Conclusion:HSP 70 is expressed in periodontal ligment at different levels in different stages during orthodontic tooth movement.

Objective To explore the risk factors for complicated hypertension in type 2 diabetic patients. Methods A community-based case-control study was conducted and some risk factors of complicated hypertension in type 2 diabetic patients were analyzed.126 cases and 273 controls were type 2 diabetic patients with and without hypertension respectively.Results The results from univariate logistic regression analysis showed that the occurrence of complicated hypertension in type 2 diabetic patients was statistically related to the body mass index(BMI),waist hip ratio(WHR) and the intake of grain crop.The corresponding OR with 95% CI were 1.474(1.101~1.973),1.478(1.180~1.852) and 1.349(1.098~1.657),respectively.After adjusted for confounding factors,the associations mentioned above were still statistically significant.No associations were observed between complicated hypertension in type 2 diabetes and diabetes duration,glycemia control,exercise,smoking and alcohol drinking,serum total cholesterol,triglycerides,high density lipoprotein,low density lipoprotein and very low density lipoprotein cholesterol levels.Conclusion The high body mass index(BMI),high waist hip ratio(WHR) and high intake of grain crop may increase the risk for the complicated hypertension in type 2 diabetic patients.

Objective To investigate the clinical and skeletal muscular pathological features of chronic progressive external ophthalmoplegia(CPEO).Methods The clinical and skeletal muscular pathological data of 6 patients with CPEO were analyzed retrospectively.Results All of the 6 patients onset were 4 to 18 years old.The first symptom was blepharoptosis.The clinical mainly manifestations were limited activity of eyeball,accompanied with limbs muscle fatigue,weakness and level elevatation of serum creatine kinase(CK).5 cases had myogenic changes in electromyography(EMG).The skeletal muscular pathological examination showed that the ragged red fibers(RRF)were scattered among the muscle fibers,and the deficient or absent of COX activity in many fibers.A few degenerating and necrotic fibers were observed in 4 cases.Lipid-drops were increased by oil red "O" stain in 3 cases.Electromicroscope showed that abnormal mitochondria increased in number and aggregated under sarcolemma or in cytoplasm.Crystalloid inclusion body could be observed.Conclusions The clinical mainly features of CPEO are external ophthalmoplegia and accompanied with limbs weakness.RRF and COX activity deficiency or absence according to skeletal muscle pathology can be suggest and support the diagnosis of CPEO.Gene sequencing based on skeletal muscle biopsy is necessary to make the final diagnosis of CPEO.

Eukaryotic elongation factor 2 kinase (eEF2K) is well known as a Ca2+/calmodulin (CaM)-dependent kinase. eEF2K catalyzes the phosphorylation of eEF2 and subsequently inactivates eEF2 by impairing its ability to bind to the ribosome, thereby negatively modulates protein synthesis. The high expression of eEF2K has been found recently in several types of malignancies. As participating in the progress of tumor, eEF2K emerges a potential target for future cancer therapy. The relationship between eEF2K and tumor, and the latest progress of eEF2K inhibitors were summarized in this article.