1.Effects of main post combined with auxiliary pile on the fracture resistance of the flat-shaped residual roots
Meng HAN ; Qiong DU ; Jing XIE ; Jingyuan LIU ; Xiao ZHANG
Journal of Practical Stomatology 2015;(4):582-584
20 first mandibular premolars were randomly divided into 2 groups(n =10).The teeth in experimental group were treated by fi-ber main post in combination with auxiliary pile,in control group by single fiber main post,and then were restored by metal crown.They were fixed in universal testing machine.The fracture load(N)of experimental and control group was (846.50 ±40.33)and (437.90 ±41.15) respectively(P <0.01).
2.Optimization of electroporation parameters in HL-60 cells for STIM1 siRNA interference during its differentiation.
Hai-Yang CHEN ; Wen-Ying ZOU ; Cui-Hua XIE ; Xiao-Jing MENG ; Chun-Qing CAI
Chinese Journal of Applied Physiology 2011;27(4):497-499
Cell Transformation, Neoplastic
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drug effects
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genetics
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Dimethyl Sulfoxide
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pharmacology
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Electroporation
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methods
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HL-60 Cells
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Humans
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Membrane Proteins
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genetics
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Neoplasm Proteins
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genetics
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RNA Interference
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RNA, Small Interfering
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genetics
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Stromal Interaction Molecule 1
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Transfection
4.Comparative study between off-pump and on-pump of coronary artery bypass grafting
Xu CAO ; Qiang XIE ; Ming JING ; Meng LI ; Xuetao BAI ; Hongwei JIANG
Chinese Journal of Postgraduates of Medicine 2010;33(2):9-10
Objective To compare the clinical effect of off-pump coronary artery bypass grafting (eft-pump CABG)and on-pump coronary artery bypass grafting(on-pump CABG).Methods The clinical dates of 152 cases with coronary artery bypass grafting(off-pump CABG group 85 cases,on-pump CABG group 67 cases)were received from June 2005 to December 2008,the preoperative dates,postoperative mortality and the rate of complication were compared between two groups.Results The preoperative dates of two groups were no significant difference(P>0.05);2 cases(2.35%)were died in off-pump CABG group and 13 cases(15.29%)had complications in off-pump CABG group;9 cases(13.43%)were died in on-pump CABG group and 25 cases(37.31%)had complications in on-pump CABG group.The mortality and the rate of complication in off-pump CABG group was significantly less than that in on-pump CABG group.it was significant difference(P<0.01).Conclusion Off-pump CABG is safe therapy of mieosurgieal operation.
5.Correlative factors analysis of affecting anatomical reattachment and vision restoration after scleral buckling surgery
Lei ZHU ; Lin LI ; Xiaoyan TIAN ; Tao LIU ; Meng CHEN ; Pei XU ; Jing ZOU ; Anming XIE
Recent Advances in Ophthalmology 2017;37(2):167-171
Objeetive To analyze the effect of treatment of rhegmatogenous retinal detachment(RRD) by scleral bucking as well as the relative risk factors affecting the anatomical reattachment and visual recovery.Methods One hundred and fortyeight patients (148 eyes) with RRD treated by sclera buckling surgery in our hospital during January 2012 to January 2016 were retrospectively analyzed.The rate of postoperative retinal anatomic reattachment,the best corrected visual acuity (BCVA) and complications were observed.Logistic regression analysis was performed to analyze the correlative factors affecting the anatomical reattachment and postoperative vision restoration.R~ults Retinal reattachment achieved in 91.9% after initial surgery and the final success rate for anatomic reattachment was 97.3% assessed with ophthalmoscope and fundus photography.But these two rates were assessed with the optical coherence tomography (OCT) were 60.1% and 80.4% respectively.Single factor Logistic regression analysis showed that retinal detachment was affected by multiple breaks and Grade C1 PVR(all P <0.05);Single factor Logistic regression analysis showed that preoperative BCVA,course of disease,retinal detachment range,macular involvement or not had an impact on the postoperative recovery of BCVA (all P < 0.05),preoperative age,refractive status,releasing retinal fluid or not,intravitreal gas injection,combined scleral buckling,and postoperative subretinal fluid,all of these factors had no effect on BCVA recovery after surgery (all P > 0.05).And through multiple factors Logistic regression analysis,preoperative BCVA was an independent risk factor for BCVA recovery after surgery (P < 0.05).Conclusion Scleral bucking is an effective technique for managing RRD,but multiple breaks and Grade C1 PVR are significant risk factors for anatomic.Preoperative BCVA,course of disease,retinal detachment range,macular involvement or not have the impact on the BCVA recovery after scleral buckling,and the preoperative BCVA is the key factor.Early diagnosis and early treatment as well as protecting the preoperative visual acuity can improve prognosis.
6.Relationship between central obesity and cardiovascular disease risk factors and resident aggregation in Yangpu District, Shanghai
Jing-jing HU ; Jia ZHAO ; Meng XIE ; Xue HAN
Shanghai Journal of Preventive Medicine 2020;32(1):36-
Objective To study the relationship between central obesity and cardiovascular disease risk factors and aggregation in Yangpu District, and to provide scientific basis for the prevention and control measures and strategies for cardiovascular disease. Methods In the community, residents aged 35 to 75 were investigated by questionnaire, physical examination and blood biochemical test, and a total of 11 321 residents with complete data were analyzed. Results The prevalence of central obesity among adults in Yangpu was 60.50%(standardized rate was 56.09%).The level of systolic blood pressure, diastolic blood pressure, fasting blood glucose, total cholesterol, triglycerides, low-density lipoprotein and high-density lipoprotein were significantly different in different waist circumference groups(all
7.Echocardiographic valuation of left ventricular diastolic function and the dynamic observation of ultrastructure and argyrophilic fiber in dabetic rats
Dongmei SUN ; Ruifeng XUE ; Shuxiang GUO ; Rui TANG ; Jing LI ; Fang XIE ; Shuhua ZHANG ; Yang LIU ; Meng SUN ; Huixue LI
Clinical Medicine of China 2011;27(11):1130-1133
Objective To explore the relationship between left ventricular diastolic function and the dynamic changes of myocardial ultrastructure and argyrophilic fiber in diabetic rats on the different periods of lesions(week 4,12 and 24).Methods The diabetes mellitus(DM)in healthy male Sprague-Dawley rats was induced by a single injection of streptozotocin(STZ,Sigma)into intraperitoneal at a dose of 65 mg/kg body weight.The left ventricular diastolic function was measured by Color Doppler Flow Imaging-Pulsed Wave(CDFIPW)and Doppler Tissue Imaging(DTI)echocardiography.Heart tissue at the apex was obtained rapidly for transmission electron microscope study.Argyrophilic staining was used in the study of argyrophilic fiber volume fraction(APFVF)in heart interstitial tissue.Results Diastolic dysfunction of left ventricle was detected in STZinduced diabetic rats by CDFI-PW(E/A < 1)at week 4,and progressed gradually.Pseudonormal filling (E/A > 1)was found in diabetic rats at week 24,which could be identified by DTI(Ea/Aa < 1).Diastolic function of normal rats was not impaired(E/A > 1 and Ea/Aa > 1).Transmission electron microscopy revealed a spectrum of subcellular remodeling in myocardium which was characterized by myofibril content decrease,disorganization,mitochondrial degeneration,sarcoplasmic reticulum,structural disorder.Compared with the control group,APFVF in myocardium was increased significantly in diabetic rats(P < 0.05).Conclusion The diastolic dysfunction in STZ-induced diabetic rats correlates with damage of ultrastructure and increase of myocardial argyrophilic fiber.
8.The protective effect of polysaccharide extracted from Laminaria japonica Aresch on vessels endothelial cell injury inducing by adrenaline.
Lu XIE ; Ai-qun LIU ; Jing LI ; Meng-hua CHEN
Chinese Journal of Applied Physiology 2007;23(2):143-147
AIMTo study the protective effect of Polysaccharide of Laminaria L01 on endothelial cell injury inducing by adrenaline.
METHODSIn order to observe the influence of L01 on the release of vWF in endothelial injured rats and HUVEC stimulated by adrenaline, a rat model of endothelial injury was established via injecting adrenaline, the damaged degree of vascular endothelial was evaluated by aortic immunity histochemistry, HUVEC was cultured in vitro, the content of vWF in rat plasma and in supernatant was measured by ELISA.
RESULTSThe measure of intact endodermis lengths (microm) stained by immunohistochemistry demonstrated the length in L01 high-dose group and low-dose group was obviously longer than that of model group (P < 0.05) in the 4th and 5th day during the model made. The content of vWF in rat plasma of L01 high-dose group was lower than that of model group (P < 0.05) in the 4th day, there were significant differences between this two groups, and the content of vWF in rat plasma of both L01 high-dose group and low-dose group was lower than that of model group (P < 0.05) in the 4th and 5th days. In the study of cultured HUVEC, on the 24 h, L01 groups (0.01 mg/ml and 0.1 mg/ml) decreased the supernatant vWF level, and on the 48 h, high-dose group (0.1 mg/ml) also decreased the supernatant vWF level, with significant difference compared with adrenaline group (10 microg/ml, P < 0.05).
CONCLUSIONL01 presented the protective effect on vascular endothelial cell.
Animals ; Endothelial Cells ; drug effects ; Endothelium, Vascular ; cytology ; drug effects ; Epinephrine ; adverse effects ; Female ; Human Umbilical Vein Endothelial Cells ; drug effects ; Humans ; Laminaria ; chemistry ; Male ; Polysaccharides ; pharmacology ; Rats ; Rats, Sprague-Dawley ; von Willebrand Factor ; metabolism
9.Considerations on the construction and reform of maternal and child health service supervision system
Jing-yi XIE ; Meng-qi CHEN ; Yi LI ; Li JI
Shanghai Journal of Preventive Medicine 2020;32(8):660-
One of the important part for ensuring maternal and child safety is to strength maternal and child health service supervision.A comprehensive supervision system for that guarantees its implementation.However, at this stage, the work faces the problems of insufficient effectiveness of laws and regulations, insufficient professional capacity of health supervisors, and insufficient supervision of emerging issues in maternal and child health service.With the implementation of "double random" spot check model in post-event supervision, primary health supervisors are facing some difficulties with the professional tasks.Combined with the situation, Shanghai municipal health supervision department established a key control point monitoring model for maternal and child health services, improving the training efficiency of primary health supervisors, offering guidance for health supervisors, which provides useful exploration in building and reforming the maternal and child health service supervision system.
10.Pharmacodynamic study of Relinqing granules on bacterial pyelonephritis model in rats.
Meng-Lin LI ; Bin LIANG ; Jing-Wen TANG ; Yu XIE ; Ai-Ping ZUO ; Feng DU
China Journal of Chinese Materia Medica 2006;31(2):153-155
OBJECTIVETo observe the effects of Relinqing granules (powder of Polygonum capitatum extract) on the bacterial pyelonephritis model in rats.
METHODThe rat bacterial pyelonephritis model was induced by injecting the escherichia coli ATCC-25922 into kidney parenchyma. The rats were divided ramdamly into Relinqing groups(52.32, 26.16 g x kg(-1)), norflorin group (0.03 g x kg(-1)), model group and normal control group, and were given experimental drugs by gastrogavage. The contents of leucocytes (WBC), occult bloo (BLD), glucose (GLU), protein (PRO), ketones, bilirubin and urobilinagen in urine were determined.
RESULTAs compared with the model group, Relinqing granules 6.0 g x kg(-1) (crude drug 52.32 g x kg(-1)) could decrease significantly the contents of WBC and BLD in urine and, however, had no markedly effects on the other biochemical parameters of urine.
CONCLUSIONRelinqqing granule has significant effects of decreasing urine WBC and BLD on the bacterial pyolonephritis in rats.
Animals ; Anti-Infective Agents, Urinary ; isolation & purification ; pharmacology ; Bilirubin ; urine ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Escherichia coli Infections ; urine ; Female ; Glycosuria ; urine ; Ketones ; urine ; Leukocyte Count ; Male ; Occult Blood ; Plants, Medicinal ; chemistry ; Polygonum ; chemistry ; Proteinuria ; urine ; Pyelonephritis ; urine ; Rats ; Rats, Sprague-Dawley