Traditional Chinese medicine is the few field of owing independent intellectual property in China, the implement of intellectual property strategy is important for the long-term development of traditional Chinese medicine enterprises. At present, traditional Chinese medicine enterprises still remain many problems in intellectual property protection, such as weak awareness of intellectual property protection, lack of intellectual property protection professional personnel, inadequate investment in R & D and low innovation level. These problems caused huge losses in practice to traditional Chinese medicine enterprises. After analyzing the status of intellectual property protection of traditional Chinese medicine enterprises, we put up with some concrete measures of intellectual property protection of traditional Chinese medicine considering the reality of these enterprises.

Objective To measure core competence of nurses by expert investigation in order to set nursing undergraduate core curriculums which matches nurse core competence.Methods 162 experts were followed with questionnaire survey and data were analyzed using cluster sampling method.Results Experts suggested that 18 core curriculums played an important role in raising nurse core competence.Conclusions Expert opinions about raising nurse core competence and offering nursing undergraduate core curriculum are intensive,study results are credible extremely,and the study offers objective evidence for making the civil standards of undergraduate nursing education.

Objective To compare the clinical efficacy of targeting arterial embolization before sacral chordoma excision and temporary balloon oc-clusion of abdominal aorta during sacral tumors surgery. Methods A total of 34 patients with sacral chordoma were recruited for this study and fol-lowed up. Totally 18 patients received bi-iliac artery,sacrococcygeal artery and other target arteryl embolization before excision(target arterial emboli-zation group),and the other 16 received temporary balloon occlusion of abdominal aorta during surgery(balloon occlusion group). The blood loss during operation,drained blood after operation,operation time and wound healing were recorded. Results The blood loss during operation,drained blood after operation and operation time in targeting arterial embolization group were less than balloon occlusion group. There was no statistical differ-ence in wound healing between the two groups. Conclusion Targeting arterial embolization can be more effective than temporary balloon occlusion of abdominal aorta in the control of blood loss.

Objective To investigate the application and advancement of hepatocyte transplantation (HCT). Methods Literatures about the advancement of HCT were reviewed and analyzed. Results There have been many novel technologies and advancements in the application of HCT. For example, gene modified cell can be used as seed cell, subcutaneous transplantation can be taken when combined with giant molecule material and the encapsulation preconditioning technique can also carried before operation to improve the rate of survival. Conclusion With more and deeper understanding of hepatocyte transplantation and the development of advanced techniques such as the application of giant molecule, HCT will be extensively used in the clinical treatment of acute and chronic hepatic diseases.

Aim To investigate the effects of Metacycloprodigiosin(MP) on tumor metastasis-associated cell behavior in vitro.Methods The effects of MP on the proliferation of HUVEC and 4TO7 cells were observed by MTT assay.Migration abilities of HUVEC and 4TO7 cells were measured by wound healing assay.Tube formation of HUVEC in the presence of MP for 10 hours was evaluated on matrigel basement membrane matrix.The abilities of adhesion and invasion of 4TO7 with MP for 24 hours were measured quantitatively by cell adhesion and transwell assay.Results MP did not affect proliferation of HUVEC and 4TO7 cells in concentration less than 10 μmol·L-1.MP inhibited HUVEC cells migration and formation of the tube in the concentrations of 0.01 to 1 μmol·L-1.In these concentrations MP could also inhibit 4TO7 cells migration,adhesion and invasion.Conclusion sThese results demonstrate that MP effectively inhibits tumor metastasis in vitro.All these findings suggest its potential as an anti-tumor metastasis compound in vivo.

D-dimer,which is degradation products during the fibrinolytic process,can reflect the fibrinolytic activity in the body.Blood shows a hypercoagulable state in cancer patients,which can directly reflect the patient's condition.It is recognized that D-dimer plays an important role in evaluating the condition of cancer patients,treatment effect and prognosis.

Objective To discuss the main complication and death cause of VLBWI,and enhance survival rate. Methods We summarized our experience and analyzed distribution of respiratory disease diseases of 68 cases of VLBWI. Results 51 cases were cured and 9 cases were died. Conclusion The respiratory disease was the main complication and death cause of VLBWI. The survival rate of VLBWI could be improved by being in NICU,intervening early with medicine and strengthening management of respiratory tract.

Objective To analyze the characteristics and diagnosis of children aged

Henoch-Schonlein purpura (HSP) is the most common vasculitis disease in childhood.It is a multisys-tern disease that most commonly affects skin,joints,gastrointestine and kidneys.The causes and pathogenetic mechanisms underlying HSP have not been understood yet.Recently great progress have been made on HSP and attention has been paid to the relationship between P-selectin and HSP.This article summarizes recent work in the advancement of P-selectin and HSP.

Objective To investigate the mechanism of the gastric epithelial cell death with stress induced damage. Methods Water immersion-restraint stress model was developed with SD rats, Apoptotic cells were quantitated in gastric mucosa by terminal deoxynucleatidyl transferase mediared dUTP nick and labelling (TUNEL) techniques and electronmicroscopy while the BCl-2/BAX proteins were detected by immunohistochemical staining. Results Comparing with the control groupe, at 2 h after the WRS, the mucosa seriously damaged, the apoptotic quantities rose significantly(P