Arsenic ; urine ; Chromatography, High Pressure Liquid ; methods ; Formamides ; analysis ; Humans ; Urinalysis ; methods

0.05),but the expression of CD19+ in the 3rd month and the 6th month were lower than that of healthy control group(Pa

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Objective To study the efficacy and adverse reactions of the treatment on different types of infantile hemangiomas (IH) with different doses of propranolol .Methods One hundred and fifty cases of IH without contraindications were randomly treated with different doses of propranolol (low dose group:1 mg?kg -1 ?d-1 ;high dose group:2 mg?kg -1 ?d-1 ) under ECG monitoring inferior half an hour to breastfeeding taking ,and stay in hospital for observation for 6 to 24 hours after the treatment . The patients returned to the hospital for review every month(ECG and blood RT ;lung function in necessary) .Results The curative effect is better on strawberry IH than that on flat IH ,also better on deeply IH than that on superficial IH .The adverse reactions oc‐curred relatively slight in the low dose group and there was no significantly statistical differences on the treatment effects between the both groups(P>0 .05) .Conclusion It is safe and effective to treat IH with propranolol .

Objective To evaluate the value in following up the patients with clipped aneurysms with the subtraction CT angiography technique.Methods 30 consecutive patients with clipped aneurysms were followed up with 64-slice spiral CT angiography (CTA) and DS angiography 10 d later postoperatively.CT images were reconstructed with volume rendering (VR) and maximum intensity projection(MIP) techniques to display the vessels.The site of the clip and patency of the parent vessel on both CT and DSA were studies independently by two radiologists.Results The clips in 27 patients were showed clearly by the subtraction CTA.Subtraction CTA demonstrated a partial remnant of aneurysm in 2,28 cases were complete occlusion.The loading vessels were free in passing in 28 cases,occlusion and narrowing of a parent vessel were seen in one respectively.The sensitivity and specificity in detecting neck remnants on MSCT angiography were 67% and 100%,and the sensitivity and specificity in evaluating vessel patency were 100% and 100%,Inter-observer agreements were 0.78 and 1.00,respectively.Conclusion Subtraction CT angiography can be used as the mainly method in following up the patients with clipped aneurysms.

A set of specific primers was synthesized according to DNA sequence of HBV found in China, the reverse transcriptase (RT) region in polymerase gene was amplified by PCR method from the serum of 3 patients with chronic HBV infection, and then the PCR products were subcloned into pGEM Teasy vectors. 13 clones were sequenced. Sequence comparison of the selected clones was made to look for the difference. After being compared, 13 sequences of RT were found different. Besides 2 clones with long sequence deletion, the different rate of RT coding nucleic acid sequences, RT and HBsAg amino acid sequences of the 11 clones is 5 1%, 4 9% and 7 5%, respectively. Many mutation types, including point substitution and deletion mutation, were found in this region. There is quasispecies population and defective HBV genome in patients with chronic HBV infection.

Objective To construct plasmid pVR1012 M as nuclei acid vaccine for hepatitis B,was constructed to immunize mice with or without plasmid pcDNA 3.1 - IL 18 to identify the effect of Interleukin 18(IL 18). Methods Polymerase chain reaction method was used to amplify the PreS2 and S region of HBV and reconstruct plasmid pVR1012 M as nuclei acid vaccine. Plasmid pcDNA 3.1 - IL 18 was used as a co stimulator. Twenty five Balb/c mice were divided into 3 groups, group 1 immunized with 100 ?g plasmid pVR1012 group 2 pVR1012 M,Group 3,pVR1012 M with pcDNA 3.1 - IL 18, respectively, every 2 weeks for 3 times. Anti HBs were detected in serum 2 weeks after each injection. Lactated ehydrogenase (LDH) cytotoxicity assay was done to analyze the cytotoxic T lymphocytes funciton. Results The positive rate and the antibody titer of serum from mice injected pVR1012 M increasing gradually with the increasing frequency of inoculation, while those from mice injected pVR1012 M and pcDNA 3.1 - IL 18(joint group) were lower than those injected with pVR1012 M alone (Difference after 3rd inoculation was significant, P

Catechol-type siderophores secreted by a strain of soil bacteria in three different medium were assayed by two high-resolution TLC.The results showed different medium had a significant effect on the secretion of catechol-type siderophores,and in three different medium strain S1 produced different catechol-type siderophores.The effect of Al~(3+) on Catechol-type siderophores by S1 were also assayed.The results showed Al~(3+)had a significant stimulation on the secretion of catechol-type siderophores.Moreover,Al~(3+)could to some extent counteract the repression of Fe~(2+)on siderophores production.In KMB medium four catechol-type siderophores were identified and all ones except for 2,3-dihydroxybenzoic acid(2,3-DHBA) had high affinity for Al~(3+).

Objective T7 cDNA phage display system and bioinformatics methods were employed to find the binding protein to the PreS1 protein of hepatitis B virus (HBV). Methods PreS1 protein was coated in ELISA plate as the target protein, and then T7 cDNA library phage display system was used to scan the binding protein or peptide. A piece of cDNA was found to have the function to bind the PreS1 protein, and the product was named as PreS1 binding protein (PreS1BP). Using BLAST in GenBank, the amino acid sequence of PreS1BP was compared in the protein sequence database. Results The amino acid sequence of PreS1BP was identified as a piece of glioma tumor suppressor candidate region gene 2 (GLTSCR2), and the length of cDNA of PreS1BP was proved to be 1436 nt. The gene was located at chromosome 19q arm (19q13.3) with a length of 11445 base pair between 10403483 and 10414989, containing 13 exons and 12 introns. Conclusion HBV PreS1BP gene could be obtained by T7 cDNA phage display system in combination with bioinformatics methods.

Molecular imaging is a newly rising branches of science which comes from molecular biology technology and modern medical imaging,and it would be the development tendency of mendiacl imaging. This article briefly introduces the basal principle of molecular imaging,the difference and correlation between molecular imaging and traditional medical imaging and the development prospect of medical imaging.