AIM: To study the microwave-assisted extraction process of forsythin from Forsythia Suspensa. METHODS: The optimization process for forsythin in Forythia Suspensa was gained by uniform design. RESULTS: The optimization process was obtained as following: 60% ethanol as solvent at the 680 W microwave power,40 min as the radiation time,5∶1(w/w) as the proportion of solvent to raw material and 30 min as the soaking time. CONCLUSION: In comparison with the traditional extraction,the forsythin content of target substance used the microwave-assisted extraction was higher than the former,and the extraction efficiency was also much better.

In current China, the quality evaluation for Chinese materia medica (CMM) is one strategic issue of enormous important questions that need to be addressed. According to the current situation and major problems on the quality control (QC) for CMM, we use chemical analysis method, pharmacological model experiment method, and the calculation method combined with the mathematical analysis model, and then propose the "correlation in 'quality, curative effect, and metabolism'" which is a new method for the QC and is based on a quality evaluation of efficacy as the core. We hope the establishment of the correlation in "quality, curative effect, and metabolism" could provide the significant references to the QC of CMM and ensure the safety and efficacy of CMM products.

Genuine medicinal materials (GMM) is the representative of high quality materials, the particular area, excellent quality, and good efficacy make it into the essence of traditional Chinese medicine (TCM). But the scientific connotation of the GMM quality and the relationship between the quality and efficacy of GMM are the emphases and difficulty in its study. The evaluation on the theory system of “correlation in quality, curative effect, and metabolism” is carried on the curative effect with GMMs and non-GMMs as research objects and the simultaneous detection of multi-components as means. The “correlation in quality, efficacy, and metabolism” is based on the combination of chemical analysis method, pharmacological model experiment, and mathematical analysis model calculation method. The quality evaluation indexes and methods of GMM are disscused and the quality of GMM is assessed by the various views of absorption, metabolism, and effectiveness of TCM. At first, the fingerprint method to identify and select the quality control components of GMM is adopted and the qualitative and quantitative methods of multi-components in GMM are established in this study. Then the “correlation of quality, curative effect, and metabolism” is experimented through the comparison on physiological animals and model animals, the constituents are comprehensively investigated. Finally, based on the mathematical models for the medicines-related methods, we can get the components with preferred quality control, which associates with the efficacy and the proportional relationship, ascertain the material basis for the efficacy of GMM, establish a scientific optimal quality evaluation system, and provide the new ideas for the research of GMM.

Objective To compare the effectiveness,indications and side effect between microplasma technology (MPT) and 2940 nm Er ∶ YAG laser for treatment of burn scar.Methods 265 patients with hypertrophic scar were treated with MPT (MPT group,N=136) and Er ∶ YAG laser (Er ∶ YAG laser group,N=129).The patients were treated 2-6 times.Results The significant response rate was 73.52 ％ and 44.95 ％ in MPT group and Er ∶ YAG laser group; the cure rate was 28.67 ％ and 15.50 ％ in MPT group and Er ∶ YAG group,respectively.There was no statistically significant difference between two groups (P＞0.05).For moderate burn scar patients (93 cases),the significant response rate was 62.79 ％ and 10.00 ％ in MPT group and Er ∶ YAG laser group,respectively.There was significant difference between two groups (P＜0.05).Conclusions MPT and Er ∶ YAG laser has been proven to be effective and safe for the treatment of burn scar.The effectiveness of MPT is superior to Er ∶ YAG laser in treatment of moderate burn scar.

AIM:To observe the effect of botulinum neurotoxin type A heavy chain ( BoNT/A HC) on the pat-tern of spinal protein expression by intrathecal injection after spinal cord injury in rats , and to explore the role of BoNT/A HC intervention in spinal protein expression and some of its mechanisms in nerve regeneration after injury .METHODS:The model of unilateral lumbar spinal cord injury was established .The effects of BoNT/A HC intervention at different doses (2 μg, 4 μg, 6 μg and 8 μg) on the general pattern of protein expression in the spinal cord tissues at the injury site and the cranial part adjacent to the injury site was measured and evaluated by SDS-PAGE and Coomassie brilliant blue staining first, and then by two-dimensional SDS-PAGE.RESULTS:The histological structure of the ipsilateral side of lumbar spi-nal cord showed obvious destruction and degradation , mainly affecting both gray and white matter of the left side of the cord.The result of SDS-PAGE with Coomassie brilliant blue staining from injured spinal cord tissue displayed that the ex-pression of some proteins after one-time BoNT/A HC treatment appeared obviously different from that without BoNT /A HC treatment.Moreover, the pattern of the protein expression affected by BoNT/A HC was similar to that of the normal spinal cord.The more detail information from two-dimensional SDS-PAGE indicated that more than 10 proteins with different mo-lecular weight and isoelectronic points were differentially expressed at day 2 and day 20 after local injection of 6μg BoNT/A HC.This altered expression actually appeared a tendency toward the pattern shown in normal group .CONCLUSION:The immediate application of BoNT/A HC at the injury site after unilateral lumbar spinal cord injury is able to affect the pattern of local protein expression .The altered protein expression by injury could be reversed back to normal or approxi -mately normal by local BoNT/A HC administration.

Objective To evaluate the efficacy of Shuxuening Injection on cerebral infarction with meta-analysis. Methods The literaturesabout the clinical trials of Shuxuening Injection for the cerebral infarction published domestically between 1999 and 2009 were retrieved,the selected literatures were metaanalyzed. Results 14 articles were included in the systemic evaluation, and 12 of them wereabout the clinical efficacy, the total samples were 1016 cases, the value of odds ratio (OR) was 0.33 with the 95% confidence interval from0.23 to 0.46 (P<0.001). The consistency of the literature was well. Conclusion Shuxuening Injection is effective on cerebral infarction.

Objective To explore the clinical efficacy of CT-Guided radioactive 125I seed implantation in treating retroperitoneal lymph node metastases. Methods Eighteen patients with retroperitoneal lymph node metastases (20 lesions in total) received CT-guided radioactive 125I seed implantation. Treatment planning system (TPS) was used to formulate the therapeutic protocol. The radioactive activity of 125I particle ranged from 1.11 × 107-2.96 × 107 Bq (0.3-0.8 mCi) and the matched peripheral dose (MPD) was 60 -100 Gy. Postoperative dosimetry was routinely performed for all the patients in one week. Postoperative D90 (90%dose received by target volume) was 53 -107 Gy. The patient’s clinical benefit response (CBR), two-month local tumor control rate and one-year survival rate were evaluated, and the complications were recorded. Results All the patients were followed up for 2 -15 months with a median time of 5 months. The one-year survival rate was 22.2%. The clinical benefit rate, overall effective rate and two-month local tumor control rate were 72.2%, 70.0% and 90.0% respectively. No serious complications occurred in all patients. Conclusion For the treatment of retroperitoneal lymph node metastases, CT-guided radioactive 125I seed implantation is mini-invasive with satisfactory short-term effect and fewer complications. Therefore, this technique is a relatively safe therapeutic means.

Adult ; Humans ; Male ; Scleroderma, Systemic ; Silicosis ; Syndrome

Objective To investigate the distribution of CYP2C9*3 and methionine synthetase(MSA2756G) genes related to drug therapy in hyperlipidemia patients of Ningxia region as well as its relation with hyperlipidemia.Methods Genotype was determined by using amplication-created restriction sites(ACRS) and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) in hyperlipidemia patients.Results Among the 180 hyperlipidemia patients of Ningxia Hui population,the frequency of CYP2C9*3 alleles was 3.33% and mutation rate in men(3.05%) was significantly higher than that in women(0.28%)(P0.05).The frequency of MSA2756G(15.83%) alleles was significantly higher than that in healthy control group(10.25%)(P

Objective To identify and quantitate the conjunctival aerobic bacterial flora from healthy people of 3 age groups in Chongqing city.Methods Samples taken with moist swabs from the lower fornix of both eyes of 33 children(9.85?0.57 years old),31 youths(21.23?0.88 years old)and 30 elders(72.97?4.94 years old)were given bacterial cultures.Positive ones were identified and colony forming units(CFUs)were counted.Bacterial quantity was expressed as the number of CFUs divided by the weight of each sample measured before and after collection.Results The elders showed a higher isolation frequency(93.33%)than children(45.45%)and youths(50.00%)which had statistically difference(P