Objective:To prepare minocycline hydrochloride sustained release tablets and optimize the formula .Methods: The method of dry granulation tabletting was used to prepare minocycline hydrochloride sustained release tablets .With the cumulative re-lease rate in 1, 2, 4 and 8 h as the index, the amount of HPMC E50 and HPMC K100LV was studied by central composite design re-sponse surface methodology .The in vitro release similarity of the sustained-release tablets and the reference tablets was compared .Re-sults:The optimized formula contained 35 mg HPMC E50 and 70 mg HPMC K100LV.The f2 for the sustained release tablets and the reference tablets in the different dissolution media was 79.06, 84.62, 75.46 and 72.95, respectively.Conclusion: Minocycline hydrochloride sustained release tablets with the formula optimized by central composite design response surface methodology meet the requirements.The results can provide evidence for the next industrial production .

Objective:To discuss the value of fetal malformations by nasal bone measurement with Ultrasound.Methods:Retrospective diagnostic analysis of ultrasound data of the five years, 41 cases of Down syndrome (DS) with pathological and chromosomal diagnosis, with double-blind method by two experienced deputy chief physician, statistical analysis by spss17.0, used the linear regression equation to describe the relationship of the nasal bone and ultrasound gestational age.Results: Normal fetal nasal bone length increased with increasing maternal gestational age, the nasal bone with ultrasound gestational age regression equation Y=0.238 +0.0246 X, F=78.65, fetal nasal bone length and gestational age were both straight-line linear correlation (r=0.801,P=0.038), with a statistically significant (P<0.05).Nasal bone was short in the corresponding gestational age and the healthy control group, 11-14-week (t=-5.378,P=0.000), 15-18 weeks (t=-2.369,P=0.029),19-22 weeks (t=-2.195,P=0.042), significant differences in the results, with a statistically significant (P<0.05).Conclusion: Scanning the fetal nasal bone in prenatal screening, accurately grasp the specific section, can find the abnormal development of the nasal bone. The developmental of nasal bone can effective response to the gestational age of the fetus; comprehensive ultrasound screening can effectively diagnose the fetal malformations.

Objective:To observe the clinical effect and safety of desloratadine citrate disodium in the treatment of allergic rhinitis ( AR) . Methods:Totally 80 patients were randomly divided into the control group and the observation group with 40 ones in each. There was no difference in gender, age, duration of allergic rhinitis, classification of serological specificity IgE between the two groups (P>0. 05). The observation group was treated with desloratadine citrate disodium tablets 8. 8mg, po, qd. The control group was given loratadine 10mg, po, qd. The treatment course was 12 days. All the patients were not given corticosteroids and other antihistamines. The clinical symptoms and signs integral, curative effect and adverse drug reactions during the treatment were observed. Results: In the observation group, 23 cases were markedly effective, 13 cases were effective, 3 cases were ineffective, one case didn’ t finish the study, and the total effective rate was 92. 30%. In the control group, the above index was 10 cases, 18 cases, 10 cases, 2 cases and 73. 68%, respectively. There was statistically significant difference in the total effective rate between the two groups (P<0. 05). The symptoms and signs integral after the treatment in the observation group was significantly decreased compared with that in the control group, and the difference was statistically significant (P<0. 05). The incidence of adverse drug reactions in the observed group was obviously lower than that in the control group. Conclusion:Compared with that of loratadine, the clinical efficacy of desloratadine cit-rate disodium tablets is better in the treatment of AR with good safety.

Objective: To observe the clinical efficacy of moving cupping on the back for patients with chronic fatigue syndrome (CFS)-related sleep disorders. Methods: A total of 60 patients with CFS-related sleep disorders were randomized into a control group and an observation group, with 30 cases in each group. The control group was treated with oral administration of fluoxetine hydrochloride capsule. The observation group was treated with moving cupping on the back, once every other day. The efficacy was observed after 4 weeks of treatment. The fatigue scale-14 (FS-14) and Pittsburgh sleep quality index (PSQI) were assessed before and after the treatment to evaluate the clinical efficacy. Results: The total effective rate was 93.3％ in the observation group, and 73.3％ in the control group. The difference between the two groups was statistically significant (P<0.05). After treatment, the improvement of physical fatigue value, mental fatigue value, and the total score of FS-14 in the observation group were statistically different from those in the control group (all P<0.05). The scores of subjective sleep quality, sleep latency, habitual sleep efficiency, use of sleeping medication, daytime dysfunction of PSQI and the total score in the observation group were improved more significantly than those in the control group (all P<0.05). Conclusion: Moving cupping on the back can significantly improve sleep disorders in CFS patients, and it has a better curative effect than oral fluoxetine hydrochloride capsules.

BACKGROUND: Some studies have demonstrated that the degradation of extracellular matrix (ECM), which matrix metalloproteinases (MMPs) participates in, plays a key step in the corneal neovascularization (CNV). Tissue factor pathway inhibitor 2 (TFPI-2), a new type serine proteinase inhibitor found recently, can effectively inhibit the activity of MMPs. Whether TFPI-2 gene transfection can influence CNV is unclear.OBJECTIVE: To investigate the effect of TFPI-2 gene transfection on CNV.DESIGN: Randomized controlled experiment.SETTING: Laboratory for Department of Surgery, Wuhan Union Hospital; Central Laboratory, the Affiliated Third Hospital of Sun Yat-sen University.MATERIALS: This study was carried out in the laboratory for Department of Surgery of Wuhan Union Hospital and State Central Laboratory of the Third Hospital Affiliated to Sun Yat-sen University between June 2004 and March 2006. Sixty healthy purebred adult New Zealand rabbits of either gender, weighing 2.5 to 3.0 kg, were involved. Preoperatively, no obvious anterior segment ocular lesion was found by slit-lamp examination. pBos-Cite-neo/TFPl-2 was kindly gifted by Dr. Zhong Ren (Department of Hematology, Union Hospital). Peroxydase blocking agent, nonimmune goat serum,mouse anti-human MMP-1, 2 and 3 monoclonal antibodies, biotin labeled goat-anti-mouse IgG second antibody (Santa cruz Company) were used in this study.METHODS: Experimental intervention: Experimental rabbit models of CNV were created in each group by silver nitrate cautery. Then, the rabbit models were randomized into 3 groups and 20 rabbits for each group. Different reagents were subconjunctivally injected via many points in each group: saline in the group Ⅰ, empty vector in the group Ⅱ, plasmid encoding TFPI-2 in the group Ⅲ. Experimental evaluation: CNV growth was observed under the slit-lamp biomicroscope.The expression of TFPI-2 in each rabbit model was detected by reverse transcription-polymerase chain reaction (RT-PCR) method 2 weeks after modeling; the expression of MMPs in corneal tissue was detected by immunohistochemical method at 3,5,7,9 and 14 days after modeling.TFPI-2 gene expression was significantly higher in the group Ⅲ than in the group Ⅱ and group Ⅰ (P ＜ 0.01); The MMP-1, 2, 3 expressions in the corneal tissue were significantly lower in the group Ⅲ than in the group Ⅱ and group Ⅰ,respectively, especially MMP-1, 3.

Objective Serum abnormal prothrombin (PIVKA-Ⅱ) level was detected in pancreatic cancer patients and its clinical value in diagnosing and treating pancreatic cancer was explored.Methods A total of 112 pancreatic cancer patients,28 benign pancreatic diseases patients and 79 healthy controls were collected from Cancer Hospital of Hubei Province.Serum PIVKA-Ⅱ level was determined by chemiluminescent immunoassay (CLIA).CEA and CA19-9 level was detected by electrochemiluminescence immunoassay (ECLIA).Receiver operation characteristic (ROC) curve was drawn and the cut-off value was set.The sensitivity,specificity and accuracy were calculated.Results PIVKA-Ⅱ level in 112 pancreatic cancer patients was 39.0 (22.61,137.67)U/L[median (quartile range)],which was significantly higher than that in 30 patients with benign pancreatic disease [23.0 (17.32,29.67) U/L] and 79 healthy controls [21.21 (17.9,25.12) U/L].The differences were statistically significant (P＜0.01).However,there was no significant difference between benign pancreatic disease patients and healthy controls.ROC analysis showed that area under curve(AUC) for PIVKA-Ⅱ,CEA and CA19-9 was 0.793,0.707 and 0.849.The cut-off value for discriminating pancreatic cancer was established at 32.4,4.7 and 27.0 U/L for PIVKA-Ⅱ,CEA and CA19-9.The sensitivity was 61.6％,38.0％ and 66.1％,and the specificity was 86.3％,96.3％ and 89.9％,and the accuracy was 71.7％,62.3％ and 75.0％,respectively.The sensitivity,specificity and accuracy of PIVKA-Ⅱ combined with CEA and CA19-9 was 90.2％,76.0％ and 84.3％.The sensitivity was obviously higher,while the specificity was lower.Serum PIVKA-Ⅱ level was associated with advanced tumor stage.There were 20 patients who were followed up.PIVKA-Ⅱ level in 15 cases were decreased after surgery or interventional therapy (50.6 U/L vs 110.5 U/L,P ＜0.001),which was increased in 4 of the five cases who received palliative care to a various degree.Conclusions PIVKA-Ⅱ detection could benefit the diagnosis and prognosis monitoring of pancreatic cancer.

Objective: To evaluate the clinical efficacy and safety of ticagrelor in the patients with acute coronary syndrome ( ACS) . Methods:A retrospective study was applied to investigate the ACS patients treated with ticagrelor in our hospital from July to December in 2015. The basic information of patients, drug administration, platelet aggregation induced by ADP, major adverse cardio-vascular events ( cardiac death,nonfatal myocardial infarction,target vessel revascularization and stent thrombosis) , and adverse drug reactions ( ADR) were recorded. The incidence of end point events was calculated and the change of platelet aggregation induced by ADP before and after the drug administration was analyzed by SPSS statistical software. Results:A total of 161 patients were collected. The incidence of cardiovascular adverse events was 1. 2%, while the incidence of adverse drug reactions was 30. 4% including bleeding (15. 5%) without severe bleeding events and dyspnea (10. 6%) with 3 severe ones. The platelet aggregation rate before and after the ticagrelor treatment respectively was (54. 96 ± 14. 654)%and(24. 37 ± 13. 183)% in 122 patients with low reaction to clopidogrel( P<0. 01). Conclusion:Ticagrelor at the recommended dose can further reduce the platelet aggregation induced by ADP. In spite of high incidence of ADR, ticagrelor has slight ADR with good tolerance.

Objective To observe the cardioprotective effects of hyperoxia liquid by comparing the changes of blood concentrations of malondialdehyde(MDA)and super oxide dismutase(SOD)in mechanical heart valve replacement.Methods Thirty patients required mechanical heart valve replacement were randomly divided into hyperoxia liquid group(Group HL) and control group 15 cases in each.From the start of skin incision(T_(0)) to(10min) after CPB,patients received 10mL/kg Ringer's injection(control group) and 10mL/kg hyperoxia liquid(Group HL) seperately.The central venous blood samples,which were collected in both groups respectively at skin incision(T_(0)),1h after start of CPB(T_1),2h(T_2) and 24h(T_3) after aorta off-clamping,were used to test the blood concentrations of SOD and MDA.Also a series of clinical data were recorded,such as the rate of heart automatic rebeat after aorta off-clamping,ventricular arrhythmia,usage of dopamine in operation and postoperation.(Results)In both groups,the blood concentrations of MDA and SOD were all within normal range before operation.MDA level at T_(2) was lower in Group HL than that in control(P

[Objective]To develop a method using HPLC for determination content of saikosaponin a and saikosaponin d of Radix Bupleuri from different areas.[Method]Column:YMC-Pack ODS-A C18(250?46 mm,5?m),mobile phase:acetonitrile-water(43∶57),detected wavelength:203nm.[Results]The regression eguations of saikosaponin a and saikosaponin d were Ya =1.223?106 X - 2.249 8?105(r = 0.999 9),Yd = 2.093?106 X - 4.786 2?105 (r = 0.999 8).The average recovery rates of saikosaponin a and saikosaponin d were 98.13 %,97.99 %.[Conclusion]The HPLC method is simply and suitable to control quantitative of radix bupleurum.

AIM:To investigate the effect of dexmedetomidine hydrochloride on hemodynamics, sedation and analgesia effect during cataract surgery for senile patients with hypertension.METHODS: Totally 90 senile patients with hypertension receiving cataract surgery were randomly divided into two groups, 45 cases in study group during the surgery by intravenous infusion of dexmedetomidine hydrochloride, 45 cases in control group were given normal saline for intravenous infusion.Blood flow dynamics index level were detected in two groups at five time points of before surgery (T0), beginning of the surgery (T1), 10 min after beginning (T2), 20 min after beginning (T3), the end of surgery (T4).Analgesia and sedation scores were compared in two groups, the incidence of adverse reactions and complications were recorded in two groups.RESULTS: Heart rate (HR), diastolic blood pressure (DBP), systolic blood pressure (SBP) increased significantly at the time points of T1, T2, T3, T4 compared with T0 time point in the control group (P<0.05).HR, DBP, SBP decreased significantly at the time points of T1, T2, T3, T4 compared with T0 time point in the study group (P<0.05), which of the study group were significantly lower than the control group at the same time (P<0.05).Analgesia scores at the time points of T1, T2, T3, T4 were showed different degrees of decline in the two groups, and analgesia scores in the study group were significantly lower than that of the control group at the same time (P<0.05).Sedation scores at the time points of T1, T2, T3, T4 were showed different degrees of decline compared with T0 time point in the control group (P<0.05).Sedation scores at the time points of T1, T2, T3, T4 were showed different degrees of rise compared with T0 time point in the study group (P>0.05).Sedation scores in the study group were significantly higher than that of the control group at the same time (P<0.05).The incidence of adverse reactions and complications in the study group was significantly lower than that of the control group (P<0.05).CONCLUSION: Application of dexmedetomidine hydrochloride to monitoring anesthesia during senile cataract patients with hypertension surgery, can stabilize the hemodynamics and has obvious sedation and analgesia effect.