Objective To study the effects of hyperbaric oxygen therapy on traumatized cerebral neurons and investigate its mechanism in attenuating cerebral damage.Methods Seventy-two rats were randomly assigned to a control group,a traumatic brain injury group or a traumatic brain injury group treated with hyperbaric oxygen. Each group was observed 1,7 and 24 days after the operation.TUNEL was used to examine the distribution of apop- tosis cells.An immunohistoehemical method was used to examine the distribution of Bcl-2 and Bax immunoreactive- positive cells in the brain tissues.Results The average percentages of both apoptosis cells and Bcl-2 immunoreac- tive-positive cells were lower in the CAl region of the traumatized brains treated with hyperbaric oxygen than in those of the traumatic injury group at each time of observation.A significant difference in Bax immunoreactive-positive cells between the two groups was also observed.Conclusion Hyperbaric oxygen therapy can significantly protect neurons against traumatic brain injury and modulate the expression of the apoptosis related genes Bcl-2 and Bax.This may ex- plain the protective mechanisms of hyperbaric oxygen therapy in treating traumatic brain injury.

Objective To summarize the media image of traditional Chinese medicine (TCM) by analyzing the TCM-related reports onNew York Times.Methods A total of 113 pieces of TCM-related reports onNew York Times during the 1980-2015 period collected and analyzed from the aspects of the amount, genres, coverage resources, reporting attitudes and main issues under the framework of content analysis.Results①The amount of TCM-related reports has been on the rise since 1980s. There was a wide variety in the genres of the reports and the American local resources account for a large proportion.②The reporting attitude in general tended to be relatively positive, though a major difference can be found in the attitude toward acupuncture and herbs.③ Eleven main issues can be categorized in the reports.Conclusion Changes could be seen in the TCM-related reports over time. Despite the fact that the media image of TCM tends to be relatively positive, the negative attitude toward herbs may hamper their development in the US. External communication of TCM is to be strengthened so as to promote the media image of TCM.

Objective To investigate the involvement of estrogen receptor(ER)? in the proliferation of ovarian clear cell adenocarcinoma by restoring ER? expression in a cell line ES-2.Methods A plasmid with full length ER? cDNA,pRSV-ER? and its negative vector control pRSV were introduced into ES-2.The cells transfected were named according to the plasmids:ES-pRSV,ES-pRSV-ER?.RT-PCR and western blot were used to detect the expression of ER? in ES-2,ES-pRSV and ES-pRSV-ER? cells.The growth activities of cells in vitro were detected by methyl thiazolyl tetrazolium(MTT)assay,and in vivo growth in nude mice was also observed.Flow cytometry was performed to show the change of cell cycles. Results The ES-pRSV-ER? cells were identified with ER? mRNA and protein expression.The growth activities of ES-pRSV-ER? were inhibited in vitro.In MTT analysis,the values of ES-2,ES-pRSV,and ES- pRSV-ER? cells were 0.78?0.05,0.81?0.06,and 0.53?0.07(the third was lower compared with the former two,P

Recently early noni-nvasive diagnostic techniques of bladder tumor are the hot resear- ches,which is significantly important for guiding clinical therapy and prognosis.This article will review the advancements of noninvasive diagnosis of bladder tumor in recent years and compare the new develop- ments with traditional exfoliative cytology test of urine,typical cystoscopy and improved cystoscopy.

OBJECTIVE: To establish a method for simultaneous determination of six alkaloid components of Sophora alopecuroides L, including cytisine, matrine, oxymatrine, sophocarpine, oxysophocarpine, and sophoridine by HPLC and determine the contents of the six alkaloids in different parts of Sophora alopecuroides L from four different areas.

The essential oil of curcuma phaeocaulis valeto n can be extracted by supercritical fluid CO2 modified with ethanol. The recov ery of active compound, curdione, could be improved when supercritical fluid ext raction with trapping by silica gel column was used and then eluted the analytes with hexane, ether and ethanol. The relative recovery of curdione increased fro m 10.7% to 34.7%.

Objective To study the effect of image quality and radiation dose by using Z -axis automatic tube current modulation(ATCM),as well as the application of automatic tube current modulation technology.Methods A control group consisting of 25 patients underwent cervical MDCT with fixed-current technique(100-220mA).Of two study groups of 25 patients,one underwent cervical MDCT using z-axis ATCM with the noise index of NI10 and tube current intensity of 80-480 mA,and the other with the noise index of NI12.5 and tube current intensity of 80-369 mA.The noise and mean tube current-time products(mAs) were recorded.Two radiologists evaluated images for diagnostic acceptability.Results All CT examinations of study and control groups were diagnostically acceptable,though objective noise was different with z-axis ATCM than with fixed current.ATCM resulted in significant radiation dose reduction(NI10,94.8?17.9 mAs;NI12.5,74.8? 18.3 mAs),when compared with the fixed current(130.4?43.1 mAs).Conclusion Z-axis Automatic mA technique has no significant change in image quality when compared with the fixed mA technique,but the radiation dose is decreased by 27%(NI10) and 43%(NI12.5),for MDCT evaluation of neck.NI has to be selected on the basis of the tissue,lesion size and nature.

Objective To investigate the prevalence of gastroesophageal reflux disease(GERD)in outpatients who visited department of internal medicine in hospitals of Zhejiang province and its risk fac- tors were analyzed.Methods The epidemiologic study was carried out on outpatients with reflex diagnos- tic questionnaire(RDQ).The data was analyzed with Logistic regression methods.Results The preva- lence of GERD in those patients was 7.28%(95% CI:6.87%-7.69%)in preliminary screening.There was significant correlation among gender,age,degree of labour intensity,status of marriage and the oc currence of GERD(P

Objective To explore the mechanism of Fuke Zhixuexiaotong Keli in rats model of incomplete puerperal involution of uterus by detecting the expression of metalloproteinases-9(MMP-9) and tissue inhibitor of metalloproteinase-1(TIMP-1) in the uterine redintegration incomplete model.Methods Seventy-two SD female rats were randomly divided into 6 groups:blank group,model group,Chanfukang group,and high-,middle-,low-dose of Fuke Zhixuexiaotong Keli group.The model of incomplete puerperal involution of uterus was established,and MMP-9,TIMP-1 gene expression level in each group were detected by in situ hybridization detection of uterine tissue.Results The expression of MMP-9 and TIMP-1 could be successfully detected in each group.The expression of MMP-9 in model group was significantly higher than the control group,and the expression of TIMP-1 was lower.The mRNA of MMP-9 gene was significantly decreased in both high-,middle-and low-dose of Fuke Zhixuexiaotong Keli group and Chanfukang group(P

Objective To study the origin of HIV-1 strains from 2 mother-to-child cases. Method DNA fragments of HIV-1 gag gene were amplified by nested PCR from the 4 target persons, and the amplified region were sequenced. Results The sequence of the specified fragment from all persons were analyzed and the strains were belonged to subtype A/E, while the sequence between mother's and child's is highly homologous. Conclusion 2 children were infected by mother-to-child transmission.