Adult virgin female mice were killed at each stage of the estrous cycle. The mid pieces of uteri were taken and fixed in 4% neutral formaldehyde, and stained with 0.5% toluidine blue at pH 0.5. It was found that mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. The mast cell number was maximum in metestrus and minimum in proestrus, and this difference was highly significant (P

Objective To investigate the spatiotemporal expression patterns and the relationship of ?-sarcomeric actin(?-SCA),?-smooth muscle actin(?-SMA) and intermediate filament protein desmin with the maturation of the prenatal and the neonatal mouse hearts.Methods Serial sections of the embryo mouse and the neonatal mouse hearts were immunostained with antibodies against ?-SCA,?-SMA and desmin.Results Ventricle and outflow tract of embryonic day(ED) 9 heart showed stronger expression of ?-SCA and ?-SMA,but desmin expression was lower.In the atrium,the expressions of ?-SCA and ?-SMA were restricted to the dorsal and ventral walls.In the sinus venosus,only a few weakly stained ?-SCA positive cells were detected.No desmin expression was found in the atrium and sinus venosus.The expressions of ?-SCA,?-SMA and desmin were increased to their highest level at ED 12.The higher expression of ?-SCA remained to the postnatal stages.After ED 12,the expressions of ?-SMA and desmin gradually decreased in different parts of the heart,but their expressions in the right ventricle persisted longer.After birth,desmin expression was mainly concentrated in the Z lines of I bands and intercalated disks.Conclusion The presence of spatiotemporal differences in the expression of ?-SMA and desmin reveals regional differences in cardiomyocyte maturation in various parts of the embryonic mouse heart.The right ventricle shows a relatively slow pace of maturation.The ?-SMA may contribute to a peristaltoid contraction pattern of the embryonic myocardium with a slow shortening speed,and a relatively higher level of desmin is required for the maturation of the sarcomere.

Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; Signal Transduction

Objective: To transfuse rhesus bone marrow derived mesenchymal stem cells (MSCs) to rhesus leucocytes antigen (RhLA) mismatched recipients, in attempt to study the immunological reaction between recipients' T lymphocytes and the skin of donor or a third party. Methods: Bone marrow aspirates were collected from femurs of donor rhesus, and the mononuclear cells were isolated and resuspended in low glucose Dulbecco's Modified Eagles Medium (DMEM) containing 2% fetal bovine serum. Phenotype of MSCs was analyzed by flow cytometry and the differentiated cells were identified by relevant specific staining. Allogeneic MSCs were given to recipients (single infusion) without preconditioning. Peripheral blood lymphocytes from recipients were co-cultured in vitro with the donor's skin or a third rhesus for 72 h before and 20 days, 40 days, and 60 days after infusion. The pathological lesions were evaluated and the keratinocyte apoptosis of the skin sections was determined by TUNEL assay. Results: Twenty days after infusion, the pathological lesion in in vitro skin explants from MSCs donor was less and the apoptosis rate was declined to (12.2±1.9)% from a pre-infusion level of (23.0±4.9)%. The apoptosis of cells in skin explants from the third party also declined to (11.8±1.4)% from a pre-infusion level of (22.0±2.0)% (P<0.05). Intensive immunological reaction was observed between recipient and donor/the third party on day 40 and day 60 after transplantation, with the apoptosis rate being (22.0±3.2)%, (22.0±2.5)% (donor) and (23.2±2.4)%, (24.0±2.6)% (the third party). Conclusion: Allogenic MSCs transfusion can decrease the immunological reaction between donors and recipients in rhesus, but this decreased reaction is temporary and reversible.

Congresses as Topic ; Humans ; Sleep Apnea, Obstructive

Adult ; Antineoplastic Agents ; therapeutic use ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Boronic Acids ; therapeutic use ; Bortezomib ; Humans ; Male ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Pyrazines ; therapeutic use ; Treatment Outcome

Objective To investigate the changes of oxidized low density lipoprotein(oxLDL) and C-reactive protein(CRP) and their correlation in patients with acute coronary syndrome(ACS),and explore their roles in the pathogenesis of ACS. Methods The plasma levels of oxLDL and CRP were measured in 95 patients with ACS and 66 control subjects by ELISA. Results oxLDL and CRP levels were significantly higher in patients with ACS than those in control group(P

ObjectiveTo explore the effects of extended nursing intervention (ENI) in nursing ability of discharged patients with intubation of T-tubes.Methods Eighty-two patients with biliary diseases discharged with intubation of T-tubes were equally divided into two groups.The patients in the experiment group were treated with ENI and those in the control group were treated with conventional nursing.Each group received routine nursing and health education before discharge.All patients were followed up by a self-designed written questionnaire.Data regarding patients’ compliance behavior,knowledge of the diseases and satisfaction were collected and analyzed.Result Significant differences were observed between the two groups,the patients in the experiment group exhibiting better compliance behavior,their knowledge of their diseases improved and the rate of satisfaction higher (P<0.05). Conclusion ENI may be effective for the discharged patients with intubation of T-tubes to improve their compliance behavior, enhance their knowledge of their diseases and raise the rate of satisfaction.

Adult ; Anemia, Aplastic ; complications ; surgery ; Female ; Hematopoietic Stem Cell Transplantation ; Hemoglobinuria, Paroxysmal ; complications ; surgery ; Humans

Cause of Death ; Female ; Hospital Mortality ; Hospitalization ; statistics & numerical data ; Humans ; Infant, Newborn ; Inpatients ; statistics & numerical data ; Male