Objective To study the protective effect of Qidan Tongmai Tablet (QDTMT) on the (injury) induced by acute myocardial ischemia. Methods Acute myocardial ischemia was induced by sc isoproterenol (Iso, 5 mg/kg) into SD rats pretreated with ig QDTMT (2 g/kg) for two weeks. Activities of Ca~(2+)-(ATPase) in myocardial mitochondria and content of Ca~(2+) in myocardium and myocardial mitochondria were determined by colorimetric method. The changes of myocardial ultrastructure were investigated by means of transmission electron microscope. Results Compared with the controls, activities of Ca~(2+)-(ATPase) in mitochondrion were strengthened in QDTMT group and the content of Ca~(2+) was decreased ((P

Objective To study the expansion and purification of human retinal progenitors cultured in vitro.(Methods Human) embryonic retinal progenitor cells(RPCs) were cultured.The characteristics of neural stem cell were observed.The expression of Nestin was examined flow cytometry(FCM) for the comparsion between the primary and secondary passages.The differentiation of RPCs had been observed after that they had been cultured in different conditions.Results Human RPCs had proliferated and formed cell clusters with a shape of neurosphere.After digestion,the scattered progenitors formed secondary neurosphere-like cell clusters.The cells in those clusters expressed Nestin and were negative for the mature retinal cell proteins.After spontaneous differentiation,the retinal progenitors expressed special proteins of mature retinal cells,including neuron specific enolase(NSE),glial fibrillary acidic protein(GFAP),protein kinase C(PKC) and Syntaxin-1.The expression of Nestin in the secondary culture passage was(39.3?18.9)%,which was significantly higher than that of the primary passage [(28.7?17.7)%](P

The paper discusses the importance of bioethics,which is a love of life.Bioethics is the ethics of love.Three parts are included in the paper: love is foundation of bioethics;love is core of existence of bioethics;love impels bioethics to further develop.Love instructs the choice of objective bioethics in the toleration circumstance.Furthermore,love can impel the construction of bioethics in the progress of medical beneficence development.

Objective To explore the association between epicardial adipose tissue (EAT) thickness and left ventricular remodeling,left ventricular dysfunction in dilated cardiomyopathy (DCM).Methods One hundred and sixteen patients with DCM (DCM group) and 76 healthy subjects (control group) were examined by ultrasoundcardiogram.Left ventricular end-systolic diameter (LVESD),left ventricular end-diastolic diameter (LVEDD),left ventricular end-systolic volume (LVESV),left ventricular end-diastolic volume (LVEDV),left ventricular end-systolic volume index (LVESVI),left ventficular end-diastolic volume index (LVEDVI),left ventricular ejection fraction (LVEF) and EAT thickness were measured or calculated,and the relationship was evaluated.Results EAT thickness in DCM group was significantly lower than that in control group [(4.7 ± 1.2) mm vs.(7.6 ± 2.1) mm],and there was statistical difference (P ＜ 0.05).The linear correlation analysis results showed there was positive correlation between EAT thickness and LVESD,LVEDD,LVESV,LVEDV,LVEDVI,LVESVI (r =0.236,0.220,0.245,0.256,0.282,0.279,P ＜ 0.05),and there was no relationship between EAT thickness and LVEF (r =0.134,P ＞0.05).Conclusions In patients with DCM,there is a correlation between EAT thickness and ventricular remodeling.There is no correlation between EAT thickness and left ventricular dysfunction.

Radiotherapy is an important treatment for malignant tumors, but it will have a negative impact on the reproductive function of young female patients.In recent years, the measures to protect female fertility are increasing, including clinical mature technologies such as embryo cryopreservation and mature oocyte cryopreservation; highly experimental technologies such as immature oocyte in vitro fertilization and ovarian tissue cryopreservation and transplantation.In addition, the application of stem cells and the proposal of artificial ovary also show great prospects in the protection of female fertility.This paper reviews the research progress of female fertility protection strategies in radiotherapy.

Objective To analyze the value of the serum BHMT for diagnosis of hepatic injury in patients with acute poisoning.Methods ELISA method was used to detect BHMT in serum samples,and the samples were detected in the sera of normal persons and patients with acute poisoning.Results 300 samples were detected in the sera of normal persons by ELISA method.The average level of human serum B HMT content in 300 samples was 9.02 ng/mL(6.19-12.84 ng/mL),and the 95 ％ medical reference value of BHMT content in healthy people ranges from 0.00 to 28.01 ng/mL.BHMT and ALT were detected in 43 samples from acute liver injury,the Spearman analysis indicating a good positive correlation between BHMT and ALT(rs =0.757 62,P＜0.001).The comparison of the content of BHMT was made between the serum samples from 20 renal damage patient and 20 normal human serum,there was no significant difference in the BHMT content between the serum of patients with renal injury and the normal human serum(P＞0.05).Conclusion The medical reference value range for of serum BHMT content in healthy population ranges from 0.00 to 28.01 ng/mL.There was a good positive correlation between BHMT and ALT.BHMT might not be closely related to kidney injury,while be more specific to liver injure.BHMT could be a better marker of acute poisoning liver injury.

IgG4 related disease (IgG4-RD) is a systemic disease which was named and accepted in recent years.IgG4-RD is a multiorgan-involved disease, mainly including pancrease, parotid gland, bile duct, liver, etc.The study on the relationship between IgG4 and orbital disease is relatively few.In recent years, our study group aimed directly to the relationship between IgG4 and common orbital disease, including benign lymphoepithelial lesion of lacrimal gland,idiopathic orbital inflammatory pseudotumor, orbital lymphoma, etc.We found that IgG4 was associated with the pathogenesis and progression of these diseases.This article addressed systematically the pathogenesis of IgG4 related orbital disease and evaluated the values of IgG4 in diagnosis,differential diagnosis,treatment and montoring of these orbital diseases based on related literature and reseaching results from our study group,in order to deepen the recognition of IgG4 related orbital diseases in ophthalmologists.

Objective To evaluate the diastole asynchrony of the different segments of left ventricle(LV) and its relation with LV diastole function in the patients with hypertrophic cardiomyopathy (HCM) by quantitative tissue velocity imaging(QTVI).Methods Forteen normal controls,15 patients with non-obstructive HCM (HCM) and 12 patients with obstructive HCM (HOCM) were studied by QTVI.The regional velocity profiles of 18 segments of LV and mitral annulus of six walls along LV apical long axis view,apical two chamber view and four chamber view were obtained.The time (T_ Q-E ) from onset of QRS complexes to peak velocity during early diastole were measured.The maximal difference in T_ Q-E with in the same wall (Intra-?T_ Q-E ),the same segment (Inter-?T_ Q-E ),all 18 segments (Max-?T_ Q-E ) of LV and the mean ratio of mitral annulus early and late diastole peak velocity of six walls were calculated(Ea/Aa′).Interventricular septum basic thickness(IVSTh) and LV outflow tract gradient(LVOTPG) were measured.Results Inter-?T_ Q-E ,Intra-?T_ Q-E ,Max-?T_ Q-E were significantly prolonged and Ea/Aa′ was reduced in HCM and HOCM groups compared with control group.There was no statistically significant difference of Inter-?T_ Q-E ,Intra-?T_ Q-E ,Max-?T_ Q-E ,Ea/Aa′ between HCM and HOCM groups.In control group,HCM group,HOCM group,IVSTh and LVOTPG were increased little by little.Max-?T_ Q-E were correlated negatively with Ea/Aa′ in patients with HCM and HOCM.IVSTh and LVOTPG were mildly correlated with Ea/Aa′ in HOCM group,but not correlated with Ea/Aa′ in HCM.Conclusions Asynchrony diastole is a neccessary factor causing impairment of LV diastole function exists within the same wall and the same segment of LV in patients with HCM,but IVSTh and LVOTPG is not a neccessary factor causing impairment of LV diastole function.Asynchrony diastole in patients with HCM could be an appereance of cardiomyopathy.

Objective To evaluate the asynchrony of right ventricle(RV) and its significance in idiopathic pulmonary artery hypertension(IPAH) patients by quantitative tissue velocity imaging.Methods Twenty-five normal people,26 IPAH patients were studied.RV structure parameter(thickness of lateral wall,late diastolic and systolic area and fractional area change) and function parameter [peak systolic velocity(Sa),early diastolic velocity(Ea),late diastolic velocity of tricuspid annulus of lateral wall and Tei index] were measured and calculated.Regional velocity profiles of 6 segments of RV were obtained.The time from onset of QRS complexes to Sa and Ea(T_(Q-S),T_(Q-E)) of 6 segments were measured.The maximal difference in T_(Q-S) and T_(Q-E) in the same wall(Intra-?T_(Q-S),Intra-?T_(Q-E)),the same segment(Inter-?T_(Q-S),Inter-?T_(Q-E)) and all 6 segments(Max-?T_(Q-S),Max-?T_(Q-E)) were calculated.Results Compared with controls,Inter-?T_(Q-S),Inter-?T_(Q-E),Max-?T_(Q-S) and Max-?T_(Q-E) were significantly prolonged in IPAH patients(P(0.05)).Max-?T_(Q-S) and Max-?T_(Q-E) were correlated with structure and function parameter in IPAH patients.Conclusions Asynchrony exists in RV and have good relation with RV structure and function in IPAH patients.

Objective:To review methodology of medicine price index methodology and provide methodological implications for empirical researches. Methods:Based on the recent original home and abroad researches, the price index formulas, the medicine product definition, price and quantity units were introduced in this paper together with their merit/demerit and different selections’ measurement bias. Results: The measurement results were significantly different along with selections of the different weighted price index formulas. The medicine product definition and the price/quantity units in the formulas were different as well. By taking into account the new products entry and the ol-der ones exit updating issue, and by considering the products quality changes issue can also have an important empir-ical implication for the price measurement. Conclusions:The medicine price index methods need to be deeply devel-oped and the researchers should devote to further research and perform accurate measurement. In the empirical analy-sis, researchers can use different methods to measure the medicine price index in order to more accurately reflect the medicine price level change.