OBJECTIVE:To investigate the influence of TCM Granules of the detoxification and dissipation blood stasis formula Ⅱon fulminant hepatic mitochondrial lipid peroxidation in rats with hepatic failure.METHODS:The fulminant hepatic failure rat models were established by subcutaneous injection of thioacetamide (TAA).48 Wistar rats were randomly divided into 7 groups:blank control group,model group,low dose group,medium dose group,high dose group,the lactulose treatment group,and the "Bezoar pill for resurrection" treatment group.Intragastric administration was executed 3 d before model-making,twice per day with the interval of 12 hours.They were administered for 11times.12 hours after model establishment.MDA、SOD、CAT、GSH、NO and liver necrosis area in hepatic mitochondria were determined.RESULTS:The detoxification and dissipation blood stasis formula Ⅱcould notably reduce liver necrosis area and restrain produce of hepatic mitochondrial lipid peroxidatide MDA,retrieve the activity of SOD、CAT and increase the content of GSH,NO.Moreover it shows dose-effect relation.Compared with model group,there is statistical significance(P

Objective To study how to fulfill the repair security works of servicing and maintenance of medical equipment when medical corps execute the mission of peacekeeping successfully. Methods The maintenance and support work of medical equipment are fulfilled from several aspects, including the strict selection of maintenance personnel, learning and preparation of equipment and spare parts before going abroad, the completion of peacekeeping mission and the principle of equipment management and how to purchase medical equipment, etc. Results The operation of equipment is ensured under normal condition and the COE verification is passed through successfully. Conclusion The security system of medical equipment management and maintenance in peacekeeping and field operation is groped.

ObjectiveTo explore the feasibility of bone marrow stromal cells (BMSC) of rats differentiating into endothelial cells.MethodsBone marrow cells were obtained by an aseptic technique. Afterwards, the obtained cells were divided into two groups: cells in test group were propagated in 1640 medium with recombinant human GM-CSF (rhGM-CSF) (400ng/ml), and that in control group were propagated in medium with 1640 medium only. The differentiated cells were detected by specific immunology marker by immunocytochemistry and immunofluorescence at day 8.ResultsThe differentiated cells demonstrated the characters of endothelial cells under phase contrast microscopy. Cells of the test groups demonstrated specific characters by immunocytochemistry stain.ConclusionBone marrow stromal cells can multiply vigorously and differentiate into cells with endothelial cells characters in the medium with high concentration rhGM-CSF.

OBJECTIVETo explore the management of fungal pyelonephritis in infants.

METHODData from 5 cases with fungal pyelonephritis, including the clinical situation, laboratory examination, feature of imaging, and treatment were analyzed.

RESULTAll the 5 cases were preterm and low birth weight infants. In 3 cases the disease was unilateral, in 2 cases were bilateral, and acute renal failure occurred. Fungus balls presented on imaging. Urine culture was positive of Candida albicans. Treatment with percutaneous nephrostomy, irrigation and antifungal agent were associated with good prognosis. Only 1 case died. The surviving patients were followed up for 10 - 20 months and the results showed normal growth and development. B-mode ultrasound examination did not show any malformation of the urinary system.

CONCLUSIONFungal pyelonephritis was commom in preterm infants. Candida albicans was the major pathogenic microorganism. Percutaneous nephrostomy and drainage were effective in patients with urinary obstruction in relief of obstruction, early diagnosis and control of infection.

Acute Kidney Injury ; etiology ; therapy ; Amphotericin B ; administration & dosage ; Antifungal Agents ; administration & dosage ; therapeutic use ; Candida albicans ; isolation & purification ; Candidiasis ; complications ; diagnosis ; therapy ; Chymotrypsin ; administration & dosage ; Female ; Humans ; Infant ; Infant, Newborn ; Infant, Premature ; Male ; Nephrostomy, Percutaneous ; Pyelonephritis ; diagnosis ; microbiology ; therapy ; Treatment Outcome ; Ultrasonography, Doppler, Color ; Ureteral Obstruction ; etiology ; therapy ; Urine ; microbiology

BACKGROUNDB7-H3 has been widely studied in the context of tumor progression in recent years, and behaves as a tumor cell marker in a variety of tumors including colorectal carcinoma. The mechanism of B7-H3 in tumor progression is complicated and not clear yet. Studies have revealed that B7 family molecules are expressed on infiltrated lymphocytes as well as tumor cells in tumor microenvironment, which indicates that different expression pattern may lead to different clinical outcomes.

METHODSThe expression of B7-H3 was detected in tissues of 98 colorectal carcinoma patients by using immunohistochemistry. Then the expression of B7-H3 on CD3(+) T lymphocytes isolated from fresh cancer tissues of 12 colorectal carcinoma patients was analyzed by flow cytometry assay. The relationship between the expression of B7-H3 on CD3(+) T lymphocytes and patients' clinical pathological parameters was demonstrated with statistical analysis.

RESULTSPatients with more CD3(+) T cell infiltration survived much longer than patients with less CD3(+) T cell infiltration (P < 0.05); B7-H3 was highly expressed by infiltrating CD3(+) T lymphocytes in colorectal carcinoma tissues. The expression of B7-H3 was found to be significantly related with lymph node metastasis status (P < 0.05), but not with the patient's gender, age, tumor size, differentiation degree, depth of tumor invasion, Dukes' stage, distant metastasis and whether or not mucinous adenocarcinoma was present (P > 0.05). Moreover, the survival time of patients with low expression of B7-H3 was obviously longer than those of high B7-H3 expression patients, but the seven-year survival rate showed no difference between the high and low B7-H3 expression patients (P > 0.05).

CONCLUSIONThe negative costimulatory molecule B7-H3 on infiltrating CD3(+) T lymphocytes in colorectal carcinoma bears importance in the clinical pathological progress and prognosis of colorectal carcinoma.

Adult ; Aged ; B7 Antigens ; analysis ; CD3 Complex ; analysis ; Colorectal Neoplasms ; immunology ; mortality ; pathology ; Female ; Flow Cytometry ; Humans ; Immunohistochemistry ; Lymphocytes, Tumor-Infiltrating ; immunology ; Male ; Middle Aged ; Survival Rate ; T-Lymphocytes ; immunology

AIM: To investigate the expression of connective tissue growth factor (CTGF) and α-SMA in human lens epithelium cell (HLEC) line B3 after transfection by liposome-coated siRNA targeting CTGF.METHODS: HLECs were transfected with small interfering RNA (siRNA) targeting CTGF,labeled with 5`-fluorescein isothiocyanate (5`-FITC) and coated with lipofectamine.The transfection ratio was evaluated via fluorescence intensity.Cell counting kit-8 (CCK-8) assay was performed to assess cytoviability of both non-transfected and transfected HLECs.Quantitative RT-PCR,cell immunochemistry and Western blot analysis were conducted to detect the expression changes of CTGF and α-SMA after transfection.RESULTS: A highly effective transfection ratio was observed in siRNA co-transfected with lipofectamine.The transfection ratio reached 95% at 24h.The proliferation of HLECs was inhibited by siRNA after 72h transfection.The expression of CTGF and α-SMA significantly decreased in HLECs after transfected by CTGF siRNA for 24h.This effect was not found in negative control siRNA.CONCLUSIONS: SiRNA targeting CTGF decreased CTGF and α-SMA expression in HLECs,which is a potential therapeutic strategy for posterior capsular opacification.

Objective To observe the combined poisonous effects of fluoride and aluminum on sex hormone of male rats.Methods Sixteen weaned SD healthy male rats aged two week were selected and divided into control group,aluminum group,fluoride group,fluorine-aluminum group,four rats in each group.All rats in the experimental groups were fed with corn collected from the prevailng areas containing different fluorine contents respectively for 90 days.Serum testosterone(T)and estradiol(E2)were detected.Results Compared separatelv with the control group[(3.317±0.635)μg/L],serum T level of fluorine-aluminum group[(15.994±6.558)μg/L]was higher(P＜0.05),but aluminum[(8.134±3.134)μg/L]and fluorine[(1.868±0.367)μg/L]groups had no significant differences(P＞0.05).Compared separately with the control group[(0.319±0.072)nmol/L],E2 level of the fluorine group[(0.172±0.030)nmol/L]being lower(P＜0.05),and it was not significant differences(P＞0.05)in the control group when compared with aluminum group[(0.282±0.012)nmol/L],and fluorine-aluminum group[(0.265±0.047)nmol/L].Fluorine and aluminum interacted with each other(F=9.82,P＜0.05).Conclusion The combined poisonous effects of fluorine and aluminum may influence sex hormone levels of male rats.

ABSTRACT:OBJECTIVE To explore the effects of Taohong Siwu Decoction on inflammatory factors and hemorheology in patients with femoral shaft fracture after surgery.METHODS 79 patients with femoral shaft fracture after surgery in the con-trol group were treated with conventional therapy while the equal number of patients in the study group were given Taohong Si-wu Decoction.The clinical curative effect,TCM syndrome,interleukin-1(IL-1),interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),whole blood high shear viscosity(ηhigh),whole blood midst shear viscosity(ηmidst),whole blood low shear viscos-ity(ηlow),plasma viscosity(ηplasm),hematocrit value(HCT),white blood cell(WBC) count and sedimentation equation-K value of both groups were compared.RESULTS The total curative effects of study group and control group were 97.02% and 79.75% respectively(P <0.01).Both groups experienced a decrease in pain,swelling,ecchymosis,IL1,IL6,TNFα,ηhigh,ηmidst,ηlow,ηplasm,HCT,WBC and sedimentation equation-K value and the treatment group was more superior to the control group(P <0.05~0.01).CONCULSION Taohong Siwu Decoction can significantly inhibit the inflammatory response and improve hemorheology indexes and clinical symptom for patients with femoral shaft fracture after surgery.

Objective To investigate the effect of third-party logistics applied on clinical nursing.Methods Third-party logistics was introduced in the hospital and provided indirect service such as collecting and delivering patients' test samples,blood products,medicine and consultation notices by non-medical professionals.Results On the introduction of third-party logistics,hospital administration became more standardized,and explicitly defined responsibilities of nurses,medical orderlies and cleaners,doctors' and nurses' satisfaction on the medical orderlies was increased from 91％ to 99％,and the differences were statistically significant ( U =5.08,P ＜ 0.01 ).The server time needed for collecting test samples,medicine,consultation sheet were significantly shorter than that of before [ ( 5.30 ± 0.48) min vs (8.30 ± 0.88) min,(13.00 ± 1.41)min vs (20.00 ±2.00) min,(4.10 ±0.21)min vs (7.20 ±0.32)min;t =6.69,6.39,18.09,respectively ;P ＜ 0.01 ].Conclusions The widespread introduction of third-party logistics is worthwhile for the hospital,which makes nurses more focusing on the direct patients' care,saves much cost for the hospital in terms of human resources,and it better ensures the timeliness and accuracy of logistics.

Objective To observe the feasibility of measuring multiple circulating cytokines by protein chips in patients with acute coronary syndrome(ACS).Methods Circulating cytokines were determined bv protein chips in 50 control cases and 104 ACS cases of unstable angina pectoris(UA,n=50)and acute myocardial infarction(AMI,n=54)cases.Results The levels of serum MMP-9,sCD40L,CRP,IL-6,H-FABP,cTnⅠ and plasma IL-8,NT-proBNP,sVCAM-1,ET-1 in ACS group were significantly higher than those in the control group(all P＜0.01).Serum H-FABP and cTnⅠ were significantly higher in AMI patients than in UA patients(P＜0.01).Serum MMP-9,sCD40L,CRP,IL-6 and plasma sVCAM-1,ET-1 tended to be higher in AMI patients as compared to UA patients(all P＞0.05).Serum MMP-9,sCD40L.and H-FABP were positively correlated with the severity of angina(r=0.653,r=0.745,r=0.933,P＜0.01,respectively).Serum MMP-9,sCD40L,and H-FABP increased in proportion to the levels of Braunwald angina classes in UA patients(angina class Ⅰ＜angina class Ⅱ＜angina class Ⅲ,P＜0.01).Conclusions Circulating cytokines were significantly increased in ACS patients and positively correlated to Braunwald angina classes in UA patients.