Anaplastic thyroid cancer (ATC) is the most aggressive thyroid tumor, with lower morbidity and higher mortality rates. Radical surgery could improve local control and survival rates. Conformal radiotherapy is superior to conventional radiotherapy, which could improve the patients’ quality of life. There are a few chemotherapeutic drugs against ATC, and the outcome of chemotherapy alone is modest. Concurrent chemoradiotherapy can prolong the survival. Targeted therapy brings new hope for refractory patients. Many cancer centers are exploring surgery, radiotherapy, chemotherapy, targeted therapy and other biological treatment of comprehensive application, in order to enhance the curative effect. This article reviewed the foregoing treatments for ATC.

OBJECTIVE: To optimize the extraction technology of Huanggan decoction. METHODS: The extraction technology of Huanggan decoction was optimized by L9(33) orthogonal design with the concentration of alcohol, the amount of solvent and the extraction time as factors taking the contents of chlorogenic acid as indexes. RESULTS: The optimal extraction process was as follows: adding six and five-fold of 70% alcohol, extracting for 60 min and 45 min. CONCLUSION: The extraction technology has been proved to be reasonable, simple and feasible, and it provides theory evidence for production.

Objective To investigate the clinical features,critical laboratory parameters,and fetal monitoring methods in intrahepatic cholestasis of pregnancy(ICP).Methods A retrospective analysis of 21 cases of ICP suffered with fetal death in Women's hospital.School of Medicine.Zhejiang University from January 1999 to December 2010 were discussed.Results(1)The average age of ICP patients suffered with fetal death were(30.2±4.6)years old.Among them,4 cases were older than 35 years,six cases were multipara.oneo of them suffered stillbirth 2 year before.Twenty cases were singleton pregnancies and 1 cage was twin pregnancy.(2)All 21 cases of fetal death occurred in the third trimester,12 cases occurred before 37 weeks,9 cases after 37 weeks.Nine cases were diagnosed by ultrasound in outpatient clinics,fetal heart beat disappeared in 9 patients after admission because of ICP, two disappeared after labor, one during anesthesia before emergent surgery. Perinatal mortality rate of ICP was 0. 148% (21/14 184), and fetal death occurred from 29 to 41 weeks with an average gestational age of ( 33.8 ± 4. 2 ) weeks, ( 3 ) Puritus occurred in all 21 cases while 11 of them had pruritus all over the body. Ten pregnant women felt the fetal movement decreased or disappeared before diagnosis of fetal death. The glycocholic acid levels increased in all of the 21 cases. Among them, glycocholic acid levels in 11 cases were (21.49 -64. 48) μmol/L, while in 10 cases were ≥64. 48 μmol/L Serum bile acid levels elevated in 16 cases which had been analyzed ( the other 5 cases had not been checked ), and the highest level reached 270 μmol/L Serum alanine aminotransferase and aspartate aminotransferase were increased in 14 cases. Seven cases had their total bilirubin ＞21 μmoL/L, and 12 cases had their direct bilirubin levels significantly elevated. Among the 21 cases of ICP, 15 cases were in severe status, while the other 6 cases were mild. (4) Nine patients had no antepartum surveillance since fetal death were diagnosed before admission. The results of antepartum surveillance were as follows: 2 cases had nonreassuring nonstress test (NST), one had mild "V" type deceleration. Absence of diastolic flow in umbilical artery were found in 3 cases, and low fetal biophysical score was got in one case. ( 5 ) All 21 patients had vaginal delivery. Six of them delivered after natural contraction, and the remaining 14 cases delivered after oral intake of mifepristone and amniotic injection of ethacridine, or oxytocin induced labor within 48 hours, only one case delivered after additional dinoprostone suppositories. The appearance of fetus, placentas and membranes were normal, the lengths of umbilical cord were average. Four cases were found with cords binding the necks or the bodies. Eighteen cases had grade Ⅲ amniotic fluid with meconium-stained, and 2 cases complicated by oligohydramnios. Ten cases had their fetuses and placentas examined by pathologist. Among them, one case had multiple malformations, no more obvious pathological abnormalities were found in other fetuses. Pathologic examination showed that fibrin deposited around chorion and deciduas basalis, large vessels accompanied by calcification, degeneration,hemorrhagic infarction, and increased focal syncytial nodules could be seen in all of the ten placentas. Conclusions Fetal death in pregnant women with ICP of ten occurs after the contractions, Severe ICP may be a key factor that involved in the occurrence of fetal death. Up to now, there is no valid indicators in fetal monitoring, which can predict fetal death. Extensive assessment of the severity and careful antepartum surveillance should be achieved before timely termination of pregnancy.

BACKGROUND: As a kind of filling material for wedge-shaped defects, GC Fuji Ⅸ glass-ionomer cement has arose more and more attention. However, the comparison of repair results between GC Fuji Ⅸ glass-ionomer cement and reinforced glass-ionomer cement are poorly understood. OBJECTIVE: To compare the clinical effect of GC Fuji Ⅸ glass-ionomer cement and reinforced gtass-ionomer cement for repairing wedge-shaped defects of old people. METHODS: Totally 80 teeth were randomly divided into 2 groups, and filled by GC Fuji Ⅸ glass-ionomer cement (experimental group) and reinforced glass-ionomer cement (control group), respectively. The clinical effect of 2 materials were evaluated on color match, edge density heterozygosity, restoration integrity, occurrence of secondary caries and pulp symptom at immediately, 3 months, 6 months and 1 year after placement. RESULTS AND CONCLUSION: The color match of the experimental group was better than that of the control group at 3 months, 6 months and 1 year after placement (P < 0.05); and the edge density heterozygosity of the experimental group was superior to the control group at 6 months and 1 year after placement (P < 0.05); in addition, the restoration integrity of the experimental group was surpass the control group at 1 year after placement (P < 0.05). It demonstrated that GC Fuji IX glass-ionomer cement is an ideal choice for wedge-shaped defects of old people, which exhibits superior effects to reinforced glass-ionomer cement in 1-year follow-up.

Objective To investigate the effects of substance P (SP) on the expression of apoptosis-associated genes in fibroblasts derived from pathological scars. Methods Fibroblasts derived from keloid, hypertrophic scar and normal skin were cultured separately in media containing SP and SP receptor antagonist. PCNA, bcl-2 and bax protein in fibroblasts were assessed by means of immunohistochemistry. Results SP enhanced PCNA and bcl-2 expression in all three kinds of fibroblasts, whereas, bax expression was inhibited significantly. SP inhibited the expression of bax in keloid scar fibroblasts (KSFB) more remarkably than that in hypertrophic scar fibroblasts (HSFB) or normal fibroblasts (NFB), and the effect was stronger on HSFB than on NFB. SP receptor antagonist could inhibit those effects of SP totally or partially. Conclusion SP may play an important role in the formation of pathological scars by modulating the expression of apoptosis-associated genes, which is mediated by SP receptor.

BACKGROUND:The large watercress molded piece is longer than that of the normal molded piece, which is suitable for dental restoration of elderly patients with gingival recession and relatively long crown.
OBJECTIVE:To test and verify the effect of large watercress molded piece on the auxiliary repair of approximal caries lesions in molars of the elderly.
METHODS:Eighty-seven senile patients with molar approximal caries were randomly divided into two groups: those were repaired by large watercress molded piece as experimental group, and those repaired by traditional amalgam molded piece ascontrol group. Then we compared the comfortable satisfaction rate, the excelent and good rate of repair and the gingival index between the two groups immediately, 1, 3, 6 and 12 months after repair.
RESULTS AND CONCLUSION:The comfortable satisfaction rate of the experimental group was significantly higher than that of the control group at 1, 3, 6 and 12 months after repair (P< 0.05), and the gingival index was significantly lower than that of the control group (P< 0.05). The excelent and good rate of the experimental group was significantly higher than that of the control group at different time points after repair (P< 0.05). No loss or fracture of the restorations occurred in the two groups. These results demonstrate that the large watercress molded piece for repairing approximal caries lesions in molars of the elderly can better restore the anatomical shape and approximal points of teeth, and reduce food impaction and gingival inflammation.

Objective To analyze the physical examination data of 557 faculty and staff working in one uni-versity,in order to provide a basis for control and prevent chronic disease in the same group people .Methods Five physical examination items including blood pressure ,blood sugar,cholesterin,triglyceride and blood uric acid were chosen and analyzed .Results The abnormal rate of high blood pressure was 20.83%,and that in males was obviously higher than the females(χ2 =26.82,P<0.05).The abnormal rate of high blood sugar was 9.69%,and that in males was obviously higher than females (χ2 =19.76,P<0.05).The abnormal rate of high blood uric acid was 16.52%, and that in males was apparently higher than the females (χ2 =10.89,P <0.05).The abnormal rate of high triglyceride was 28.55%,and that in males was obviously higher than females (χ2 =53.92,P<0.05).The abnormal rate of high cholesterin was 42.55%,there was no significant difference in gender (χ2 =0.00,P>0.05).Conclusion The health situation of faculties and staff is not optimistical .It is urgent to strengthen health education in these people .

Objective To establish a high performance liquid chromatography (HPLC) method for the determination of geniposide in Diaojinyao powder. Methods The HPLC system consisted of the Fortis-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of HCN∶H2O (12∶88) , the flow rate was 1.0 ml/min, the column temperature was 30℃, The UV detector was set at 238 nm. Results The linear response range was 0.049~0.980mg/ml ( r=0.999 9). The average recovery of geniposide was 99.1%, and RSD was 1.66%. Conclusion The method is simple, rapid, accurate and repeatable. It can be applied in determination of Geniposide in Diaojinyao Powder.

Current therapies for chronic hepatitis caused by hepatitis C virus (HCV) are limited . DNA vaccination may constitute a new immunotherapeutic strategy for various hepatitis virus infections by promoting specific cellular and humoral immunity. In this review we present the advances in choice of antigen gene, vaccine adjuvants and the methods of intracellular delivery, unresolved questions and therapeutic potential of HCV DNA vaccine.

Objective To explore the application value of uterine incision suture in multiple segment shrinkage in cesarean section.Methods 85 pregnants with uterine scar who taken cesarean section were selected.In the single month,the pregnants were included in the observation group(43 cases):after pruning scars,uterine lower segment weak muscle tissue by wrinkling suture to reinforce the thickness of the lower segment again from both sides of the suture,intermediate knotted suture the uterine incision.In the bimonth,the pregnants were included in the control group(42 cases):after pruning scars by conventional suture incision of lower uterine segment.The duration of vaginal bleeding time after operation ,postoperative 42 days ,3 months ultrasonography of uterine incision healing were observed.Results The postoperative vaginal bleeding volume of the observation group was less than that of the control group[(480.55 ±53.75)mL vs.(550.14 ±50.45)mL],the bleeding time was less than that of the control group[(21.31 ±6.78)d vs.(30.45 ±5.16)d],the differences were statistically significant(t =3.65,2.28,all P <0.05).March B -ultrasonography uterine incision after surgery,the good healing rate of the observation group was 95.35%,which of the control group was 78.57%,the difference was statistically significant(χ2 =4.35,P <0.05). Conclusion Incision under section shrinkage joint is conducive to the incision structure and muscle layer mechanical recovery and can reduce the amount of vaginal bleeding and time and defect healing of incision morphology.