Objective To investigate the type 2 diabetic patient's periodontal condition and to analyze the influencing factors of periodontitis.Methods A total of 182 type 2 diabetic patients were included in the survey and requested to fill out a questionnaire,and their periodontal status was evaluated by measuring probing depth(PD),attachment level(AL),sulcus bleeding index(SBI),simplified oral hygiene index(OHI-S).Results The prevalence of periodontitis in this group of patients was 96.7%(176/182),including 20 patients with mild periodontitis.156 with moderate to advanced periodontitis.The mean PD and AL of the 182 patients were(2.92±0.67)rain and(2.87-4-1.31)mIIL At least one tooth Was lost in57.1%(104/182)of the patients.The factors related to periodontitis were age,gender,smoking,living in town or country,and 2 h plasma glucose of oral glucose tolerance test(OGTT).There Was no relationship between the severity of periodontitis and education level. The majority of patients did not receive any periodontal therapy.Conclusions Periodontal status was bad in patients with type 2 diabetes.It is important to develop an education program on oral health for tvpe 2 diabetic patients.

Objective To investigate the effects of F protein of hepatitis C virus subtype lb on the apoptosis of human hepatocellular carcinom HepG2 cells. Methods HepG2 cells were transfected with recombinant plasmid pcDNA3.0-F-EGFP and pcDNA3.0-F-EGFP-HepG2 strain was exposed to Act-D and tumor necrosis factor a (TNFα) treatment in order to induce cell apoptosis with positive control pcDNA3.0-C-EGFP-HepG2, negative control pcDNA3.0-C-EGFP-HepG2 and blank control HepG2.Annexin V-FITC/PI of flow cytometry was performed to determine the number of apoptotic cells. DNA Ladder was used to observe the isolation of apoptotic DNA fragments in the apoptotie cells. Results pcDNA3.0-F-EGFP- HepG2 cell strain showed a much delayed apoptosis as well as obviously lowering the apoptotic rate when compared with the pcDNA3.0-HepG2 strain and HepG2 strain (P<0.001).Conclusion The introduction and expression of extraneous gene (the F gene of hepatitis C virus subtype 1b) could significantly inhibit the apoptosis of HepG2 cells.