Chromatography, Gas ; methods ; Dimethoate ; blood ; Humans ; Insecticides ; blood ; Methyl Parathion ; blood

Objective To study the effect of bacterial culture and susceptibility test for chronic prostatitis.Methods 108 cases of chronic prostatitis patients with prostatic fluid were separated culture and sensitivity test.Resuits The bacteria masculine rate was 67.4% in the group,it was in 71.3% in Gram-positive cocci,it was 17.6% in Gram-negative bacilli,it was 3.7% in Gram-positive baciUi and it was 7.4% in Fungi; Staphylococcus anreus,Coagulase negative staphylococcus,Streptococcus viridans,Enterococcus faecalis and Bacillus coil were drug fast to frequently used antibiotic on the wide-bound.Conclusion The bacterial infection has an important role in the invasion of chronic prostatitis and the incidence rate was high in the bacterial multidrug resistant.

Objective To observe the distribution of cornea Q value in adult myopia,and its relationship with age,sex,eye and refraction.Design Prospective case series.Participants Consecutive 510 patients (1020 eyes) of myopia of adults.Methods Cornea Q values were obtained with OrbscanⅡz Anterior Segment Analysis System in all subjects of adult myopia.Normal distribution test of Q value and correlation analysis with sphere,cylinder,sex,and age were performed.Main Outcome Measures Q value of cornea,corre- lation coefficient.Results In 1020 eyes of adult myopia,the mean Q value was-0.27?0.16 (range from-0.86 to 0.25) with near nor- mal distribution.The difference of Q value between right eyes and left eye was not statistically significant (P=0.675).The Q value be- tween male (-0.26?0.15) and female(-0.27?0.17) was not statistically difference(P=0.697).No significant relation was found Q value with either sphere (r=0.057,P=0.068) or cylinder (r=0.044,P=0.156).Mean Q value of below 20 years old was maximal,-0.24,while over 40 years old was minimal,-0.36,and the difference were statistically significant (P=0.000).Conclusions Q value of cornea has negative shift with the increase of age,especially in over 40 years old.Q value of cornea has no relationship with left or right eyes,sex and refraction,but it is highly customized.

Objective To compare the effects of neutral posture training (NPT) and gas resistance training on balance and motor func-tion in stroke patients. Methods From June, 2014 to February, 2016, 44 hemiplegic patients were randomly divided into control group (n=22) and experimental group (n=22). The control group received conventional decubitus trunk muscle training and sitting gas resistance train-ing. The experimental group received NPT. They were assessed with Fugl-Meyer Assessment-Upper Extremities (FMA-UE), Fugl-Meyer Assessment-Lower Extremities (FMA-LE), and Berg Balance Scale (BBS) before and after training. Results There was no significant differ-ence in the scores of FMA-UE, FMA-LE and BBS between two groups (t<0.962, P>0.05). The scores significantly increased after training in both groups (t>12.248, P<0.001), and were higher in the experimental group than in the control group (t>1.972, P<0.05). Conclusion The NPT could improve the motor and balance function of stroke patients, rather than gas resistance training.

ObjectiveTo study the expression of telomerase of fibroblasts in the formation and development of scar,and to investgate the relationship between the telomerase activity and the formation and development of scar.MethodsExpression of telomerase was detected by immunohistochemical techniques in 18 specimens from granulation tissue,17 keloid,16 specimens from hypertrophic scar,28 specimens from mature scar,32 specimens from normal skin,and SPSS16.0 statistics software was used to analyze the relationship between telomerase and scar.Results It was shown that the positive expression rate of telomerase in the granulation tissue group was 94.4 ％,that in the keloid group was 58.8 ％,that in the hypertrophic scar group was 18.8 ％, and that in the mature scar and in the normal skin was zero. comparisons between groups,in addition to the normal scar group and normal skin group,other groups were statistically significant differences.ConclnsionsScar formation is a process with multi-factor participation,while telomerase activation is an important factor. Detecting telomerase activity in the development process of scar formation may determine the prognosis.Through the control of telomerase activity in the pathogenesis of scar may become a new approach of scar treatment.

Objective: To summarize the experiences and problems in economic management of county-level hospitals in Yulin area, provide decision foundation for the further reform of the county health service system in China. Methods: Using descriptive study to analyze the economic operation condition of the 5 pilot county-level hospitals. Results: The effect and issues of the reform both exist in financial input, pharmacy income, asset utilization and operation capabilities in pilot county-level hospitals. Conclusion: It is needed to deepen the operation mechanism and compensation mechanism of public hospitals, reform the “using drug to compensate the medicine” mechanism, strengthen the hospital asset management and optimize liability structure.

Taurine is one of the extremely important amino acids in the body,and is also the most abundant free amino acids in the central nervous system(CNS).Taurine as a conditional essential amino acids exerts a wide range of physiological and pharmacological effects.Taurine,especially as a neurotransmitter in the developing CNS,can maintain the structural integrity of the membrane,regulate calcium transport and calcium homeostasis,also as nutritional factors,osmolyte,neuromodulator,neuroprotective agents,plays an important role.In this paper,physiological and biochemical properties of taurine,source and distribution in vivo,synthesis and metabolism,absorption and transport,and its protective effect on the CNS are reviewed.

A total of 183 patients of ectopic pregnancy due to tubal factors were divided into the methotrexate (MTX),conservative surgery and salpingectomy groups.The dose of gonadotropin,counts of harvested oocytes and high-quality embryos and pregnancy rate were compared among three groups to analyze the differences of in vitro fertilization and embryo transfer (IVF-ET).And the above parameters showed no significant differences (P ＞0.05 ).The clinical pregnancy rate of the conservative group was higher than the other two groups.And the difference was statistically significant compared with salpingectomy ( P ＜ 0.05 ).It suggested that the treatments of ectopic pregnancy had some effects on the outcome of IVF-ET.The pregnancy rate was slightly higher in the conservative surgery group.

Mitochondria DNA was isolated from darkgrown shoots of Xi Feng A according to the procedure reported previously. Mitochondria DNA was digested with EcoR1 completely, and ligated with pUC19 vector DNA which was digested with EcoR1. The ligated DNA was transformed into E. coli JM101. The transformants were selected on LB medium which containing ampicillin 50ug/ml. All of the bacterial clones were hybridized with 32P-labelled maize 26S rRNA gene, seven positive clones were obtained. The plasmid DNAs were isolated from the clones and digested with EcoR1 and southern transferred to NC paper. The membrance was hybridized with maize 26S rRNA probe. One clone which a shortest hybridization band (1.3kb) was named pXMT1.

ObjectiveTo determine the optimal timing of antenatal taurine supplementation to improve neuron and neural stem cell proliferation in fetal rats with intrauterine growth restriction.Methods Twenty-five pregnant SD rats were randomly divided into five groups (five rats in each group): group A was the control group, group B to E were the fetal growth restriction (FGR) model groups with low-protein diet during the experiment, group C, D, and E were supplemented with taurine [300 mg/(kg·d)] at day 9, 11 and 15, respectively. The birth weight of newborn rats was measured after natural delivery. The rats with body weight two standard deviations lower than the average weight in group A were diagnosed as FGR. There were five litters of newborn rats in each group, and two were randomly selected from each litter, resulting in ten newborn rats in each group. Proliferating cell nuclear antigen (PCNA) and fatty acid binding protein 7 (FABP7) positive cell expression in newborn rat brain tissues were detected by immunohistochemistry. Single factor analysis of variance, LSD tests were used for statistical analysis.ResultsThe average birth weight of newborn rats in group A, B, C, D and E were (6.61±0.45), (4.65±0.23), (5.37±0.17), (5.74±0.21), and (5.00±0.24) g, respectively. Average birth weight was lower in group B than in group A (t=2.447), higher in group D and E than in group B (t=2.306 and 2.306), higher in group D than in group C and E (t=2.306 and 2.306), and the differences were statistically significant (allP<0.05). The positive expression of PCNA in the brain of neonatal rats was mainly located in the nucleus. The number of PCNA positive cells in each high power field (×400) in group A, B, C, D and E was 31.03±5.38, 46.49±4.38, 59.65±5.37, 67.76±5.84, and 53.53±6.94, respectively. The number of PCNA positive cells in group B was higher than that in group A (t=2.110), higher in groups C, D and E than in group B (t=2.110, 2.110 and 2.131), higher in group D than in group C and E (t=2.101 and 2.110), and the differences were all statistically significant (allP<0.05). The positive expression of FABP7 was mainly located in the cytoplasm of neonatal rat brain. The integral optical density (IOD) in group A, B, C, D, and E was 451 733.1± 141 452.3, 207 232.2±60 525.2, 333 766.6±68 412.1, 380 647.4±131 145.9, and 278 967.1±127 630.7, respectively. The IOD in group B was lower than that in group A (t=3.165,P=0.000), higher in groups C, D and E than in group B (t=5.272, 7.132, 2.950), and the differences were statistically significant (allP<0.05). Although the IOD in group D was higher than that in group C, the difference was not significant (t=1.953, P>0.05). The IOD in group D was higher than that in group E, and the difference was statistically significant (t=4.182,P<0.05).ConclusionsAntenatal taurine supplementation can promote neuron and neural stem cell proliferation in rats with FGR. The effect is most obvious on the 11th day of pregnancy, and may lead to the promotion of brain development.