Objective To investigate the connotation of nursing competency of nurses nursing patients with craniocerebral tratuna, and to develop the Scale of Competency of Nurses Nursing for Craniocerebral Trauma Patients (SCNNCTP). Methods A self-designed nursing competency scale for nursing persons in nursing patients with craniocerebral trauma was primarily made, through document search and questionaire investigation, and was used to survey and analyze the nursing staff conducting the nursing work to patients with craniocerebral trauma in 18 hospitals located in 9 provinces and cities through this country (n = 586), in order to establish the formal scale format of SCNNCTP. The formal scale format was re-tested (n =232), and the Chinese Nurse Competency Scale (CNCS) was used as associated criterion to determine this scale's external validity. Results SCNNCTP was a 21-item scale, including 6 factors. Single factor analysis showed that all factors loading to its item were 0.633～0.870 (P <0.01), Pearson Correlation were 0. 508 ～ 0. 708, index of internal consistency α = 0. 930, and Splite-Half Coeffcient was 0. 935, indicating that the SCNNCTP had good reliability and validity. Pearson Correlation = 0. 636, supporting the external validity of the SCNNCTP. The significant difference between nursing persons having different academic degree on the score of the SCNNCTP was found ( P < 0. 01 ). Conclusions SCNNCTP was proved to have good psychometric characteristic and have a great power of discrimination of the achievement of nurses nursing patients with craniocerebral trauma.

Objective To investigate the influence of probiotics on gastrointestinal motility and hormone secretion of rats with severe brain injury. Methods Severe brain injury rats were prepared. 54 Sprague-Dawley rats were randomized into three groups: group A ( sham-operation), group B ( standard enteral nutrition) and group C (standard enteral nutrition plus probiotics). Each group was equally divided into three subgroups on 1 d, 3d, 7 d, respectively. Gastric emptying rate, intestinal transmission rate, motilin ( MTL) and calcitonin gene related protein (CGRP) level in plasma were measured 1 d, 3 d, 7 d post trauma. Results Compared with group A, gastric emptying rate and intestinal transmission rate decreased significantly on 1 d post trauma(P <0. 01). They were still much lower in group B on 3 d post trauma(P < 0. 01), while they increased significantly in group C on 3 d post trauma(P <0.05). MTL and CGRP level in plasma increased significantly after severe brain injury; however, the levels in group B were much higher than those in group C on 1 d and 3d(P<0.01). There was no significant difference among the three groups regarding gastric emptying rate, intestinal transmission rate, and MTL and CGRP level. Conclusions Severe brain injury could induce disorder of gastrointestinal motility and dysfunction of hormone level. Early enteral nutrition supplemented with probiotics could improve gastrointestinal motility and transmission and regulate highly increased gastrointestinal hormone after severe brain injury.

Objective　To investigate the occurrence of postural hypotension (PH) in patients suffering from type 2 diabetes mellitus with or without hypertension (DMH or DM), and the relationship of PH and diabetic neuropathy, hyperinsulinemia and insulin resistance. Methods　A total of 30 cases of type 2 DM and 30 cases of DMH were included in this study. The blood pressure of all subjects were measured in supine and standing body positions respectively and PH was defined as a decline from supine to standing was ≥20 mmHg in systolic blood pressures (SBP). The concentrations of blood glucose and plasma insulin were measured to calculate the insulin sensitive index (ISI). Autonomic and peripheral function was determined by measuring the postural heart rates and the conduction speeds of superficial peroneal and communicating branch of peroneal nerves etc respectively. Results　Significant difference (P<0.01) was found in the occurrence of PH in the patients with DM (40%) and those with DMH (67%). The changes of postural blood pressure were more obvious in those with DM+PH and DMH+PH than in those with simple DM (P<0.01). The conduction speeds of newes were significantly lower in those with DMH+PH than with simple DM (P<0.05), but the occurrence of autonomic neuropathy had no difference between the 2 groups. There was no difference in postural heart rate, body mass index and blood glucose levels in fasting and 2 h after meal among the DM, DM+PH and DMH+PH groups. The concentrations of plasma insulin of those with DMH+PH were significantly higher, but their ISI significantly lower than those of the patients with DM respectively (P<0.01). The decline of postural SBP in patients with DMH+PH had a significantly positive correlation with their plasma insulin levels in fasting condition (r=0.689, P<0.01). Conclusion　The patients with DMH are more prone to PH compared with those only with DM and PH damages their peripheral nerves. Most of diabetic patients with PH suffer from obvious IR and hyperinsulinemia, and if with hypertension, the above metabolic disturbances are more severe.

Objective To observe the influence of cognitive behavioral intervention combining cancer pain self-relief with pharmacological therapies on pain and cancer pain self-efficacy. Methods Thirty patients suffering from cancer pain were enrolled into the study and self-contrast experiment was made on each patient.The experiment included two stages, in the first stage, the routine nursing was conducted from the first day to the seventh day; in the second stage, the cognitive behavioral intervention was implemented along with the routine nursing from the eighth day to the twenty-first day. The intervention consisted of cancer pain health education,skill learning, exercises and emotional support. The health education and skill learning were instructed by the researchers face to face three times per week, lasting thirty minutes each time. The changes of patients' pain management before and after health education was evaluated with by Barrier Questionnaire (BQ-L). The pain assessment, analgesic drug analysis and cancer pain self-efficacy were evaluated on 0, 7, 21 day, respectively.Results At the end of the first stage, the dosage of morphine, the number of patients taking drugs on time increased more obviously than the baseline, but there were no statistical significances in the terms of pain selfefficacy, pain intensity, while the time that the pains lasted decreased obviously ( P ＜ 0.05 ); at the end of the second stage, the dosage of morphine, the number of patients taking drugs on time increased more significantly than those at the end of the first stage, pain self-efficacy enhanced remarkably, while pain intensity and the time decreased obviously ( P ＜ 0. 05 ). Conclusions The cognitive behavioral intervention implemented on patients suffering from cancer pain can obviously enhance patients' cancer pain self-efficacy and improve patients' pain management by using the comprehensive cancer pain management such as health education, skill learning,exercises and emotional support.

OBJECTIVEHemophilia A is an inherited bleeding disorder caused by defects in factor VIII (FVIII) gene. In the present study, the frequencies of the microsatellite alleles at introns 13 and 22 in the factor VIII gene were analyzed in the group of Han nationality in Guangxi Zhuang Autonomous Region to explore their diagnostic value for hemophilia A. These two sites were also used to detect the carriers in 13 hemophilia A families.

METHODSNinty-one individuals of Han ethnic group in Guangxi Zhuang Autonomous Region (135 X chromosomes) and 13 HA families were subjected to molecular studies. First, these two fragments were PCR amplified simultaneously. Then, silver staining was used later to show their polymorphisms. The investigators selected one sample at random to obtain its lengths of the PCR products at these two sites by ABI310 PCR amplifier. After counting its repeated numbers of (CA) according to the documents concerned, the repeated numbers of the other samples could be counted easily.

RESULTSIn the 91 individuals, 6 and 4 alleles were detected at these two sites, respectively. At intron 13 the allele frequencies ranged from 0.0002 to 0.5408 and polymorphism information content (PIC) was 0.5899. At intron 22 the allele frequencies ranged from 0.0444 to 0.4963 and its PIC was 0.5359. The actual heterozygosity for intron 13 and intron 22 were 0.6364 (28/44) and 0.5227 (23/44), respectively. In 13 hemophilia A families with positive history, 9 of them were diagnosed by this method and the diagnosis rate was 69%.

CONCLUSIONWith high PICs, (CA)n at intron 13 and intron 22 were two valuable sites in the diagnosis of hemophilia A in the population of Han ethnic group in Guangxi Zhuang Autonomous Region. Compared with some other HA restrictive fragment length polymorphisms (RFLP), intron 22 (GT)n (AG)n was more informative.

Alleles ; Amplified Fragment Length Polymorphism Analysis ; Asian Continental Ancestry Group ; genetics ; Factor VIII ; genetics ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; Hemophilia A ; diagnosis ; genetics ; Heterozygote ; Humans ; Introns ; Male ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Sequence Analysis, DNA ; Silver Staining

Objective:To evaluate the efficacy of artificial intelligence assisted pulmonary nodule diagnosis system in detection pulmonary nodule and predicting the malignant probability of pulmonary nodule.Methods:A retrospectively analyze the clinical data of 199 patients with lung nodules in the Thoracic Surgery Department of Lanzhou University Second Hospital from May 2016 to July 2020. The preoperative chest CT was imported into the artificial intelligence system to record the detected lung nodules, to measure nodal diameter and density classification and malignant probability prediction value of each nodule. The detection rate of pulmonary nodules by artificial intelligence system was calculated, and the sensitivity, specificity, positive likelihood ratio and negative likelihood ratio of artificial intelligence system in the differential diagnosis of benign and malignant pulmonary nodules were calculated and compared with manual film reading. and the sensitivity and specificity in the differential diagnosis of benign and malignant pulmonary nodules under the condition of different size and density of pulmonary nodules.Results:A total of 204 pulmonary nodules were pathologically diagnosed by surgical resection, and the detection rate of pulmonary nodules by artificial intelligence system was 100%. The artificial intelligence system can distinguish benign and malignant pulmonary nodules with a sensitivity of 95.83%(95% CI: 0.8967-0.9883), specificity 25.00%(95% CI: 0.1717-0.3425), and a positive likelihood ratio of 1.27(95% CI: 1.14-1.44), negative likelihood ratio 0.17(95% CI: 0.06-0.46), Manual reading for the differentiation of benign and malignant pulmonary nodules has a sensitivity of 87.36%(95% CI: 0.7850-0.9352), specificity 72.17%(95% CI: 0.6214-0.8079), and a positive likelihood ratio of 3.14(95% CI: 2.26-4.37), the negative likelihood ratio is 0.18(95% CI: 0.10-0.31). 5mm≤diameter of pulmonary nodule<10 mm, sensitivity 100%(95% CI: 0.6637-1.0000), specificity 50.00%(95% CI: 0.01258-0.98740), 10 mm≤diameter of pulmonary nodule <20 mm, sensitivity 94.29%(95% CI: 0.8084-0.9930), specificity 29.83%(95% CI: 0.1843-0.4340), 20 mm≤ diameter of pulmonary nodule ≤30 mm, sensitivity 96.15%(95% CI: 0.8679-0.9953), specificity 18.37%(95% CI: 0.0876-0.9953), sensitivity of subsolid lung nodules: 100%(95% CI: 0.9051-1.0000), specificity 20.00%(95% CI: 0.0051-0.7164), solid lung nodule sensitivity 93.22%(95% CI: 0.8354-0.9812), specificity 25.24%(95% CI: 0.1720-0.3476). Conclusion:The artificial intelligence assistant diagnosis system of pulmonary nodules has a strong performance in the detection of pulmonary nodules, but it can not meet the clinical requirements in the differentiation of benign and malignant pulmonary nodules. At present, the artificial intelligence system can be used as an auxiliary tool for doctors to detect pulmonary nodules and assist in the diagnosis of benign and malignant pulmonary nodules.

OBJECTIVE: To investigate the effects of Da-Cheng-Qi Decoction (DCQD, ) combined with Lactobacillus acidophilus (LA) on the recovery of gastrointestinal (GI) function in traumatic brain-injured (TBI) mice. METHODS: A total of 150 male C57BL/6 mice were randomly divided into sham-injury, normal saline (NS), DCQD (0.4 mL/day), LA (⩾1 × 10 cfu/day LA), DCQD+LA (LA administration at the same dosage after 4 h of feeding DCQD), and ½ DCQD+LA groups (LA administration at the same dosage after 4 h of feeding ½ DCQD dose) by a random number table, 5-8 mice in each group. The sever TBI model was constructed according to Feeney's enhanced gravitational forces of free falling. On days 1, 3, and 7 post-TBI, plasma diamine oxidase (DAO) and D-lactic acid levels were assessed by enzyme-linked immunosorbent assay (ELISA). Occludin expression in the intestinal epithelium was assessed by Western blot analysis. Transmission electron microscopy (TEM) was used to observe the morphological changes in the network structure of interstitial cells of Cajal (ICC) and change of enteric nervous system-ICC-smooth muscle cell (ENS-ICC-SMC). Immunofluorescence staining was used to detect changes in the network structure of the ICC. RESULTS: Compared with the NS group, occludin expression in the DCQD+LA group significantly increased on Day 1, 3, and 7 post-TBI (P<0.05 or P<0.01). The concentration of DAO significantly decreased in the LA, DCQD, and DCQD+LA groups on Day 3 and 7, whilst the D-lactate concentrations in the LA and ½ DCQD+LA groups decreased on Day 1 and 3 post-injury (P<0.05 or P<0.01). The NS group experienced a great damage on the ENS-ICC-SMC network morphology and ICC network structure, and all treatment groups had some improvements, among which the DCQD+LA group presented relatively intact network morphology. CONCLUSIONS DCQD combined with LA treatment could effectively repair the intestinal mucosal barrier and improve GI motility in mice after TBI. The combination of DCQD and LA was more effective than their respective monotherapies.

【Objective】 To evaluate the cerebral hemodynamic changes due to interictal epileptic discharges(IEDs) and explore the associated neuroelectrophysiological factors and cognition deficits on electrical status epilepticus during sleep(ESES) through real-time transcranial Doppler ultrasound-video-electroencephalogram(TCD-vEEG) . 【Methods】 Eighteen ESES patients from August, 2017 to March, 2019 were recruited to undergo TCD-vEEG. The trend curve of mean cerebral blood flow velocity(MCBFV) was generated and analyzed. The spike wave index(SWI) and various TCD parameters during non-rapid eye movement(NREM) sleep were measured. The patients were divided into three clinical level groups based on seizures, their cognitive functions and the Activity of Daily Living Scale. According to the patterns of EEG during pre-ESES, the patients were also separately grouped to three groups: bilateral synchronous epileptogenic foci(BSEF), bilateral asynchronous epileptogenic foci(BAEF) and multiple epileptogenic foci(MEF). The patients were also separated into near-ESES and asymmetric ESES groups based on EEG patterns during ESES. We then performed Fisher's precise test, an analysis of variance(ANOVA) and Student's t-test, to determine that those parameters significantly varied according to clinical level and/or EEG pattern through SPSS 17.0. 【Results】 SWI was(85.22±10.33) % on average; MCBFV oscillations during deep sleep was(17.98±7.27) % on average(t = 7.579, P < 0.01); the mean of MCBFV(MCBFVm) was(92.81±21.53) cm/s on average(t = 6.464, P < 0.01); all increased significantly more than those of healthy children. SWI(F = 3.996, P < 0.05) revealed a statistically significant difference among three clinical level groups. ESES pattern had no obvious relation with the SWI, but Near-ESES influenced MCBFV oscillations during deep sleep significantly more(t = 2.885, P = 0.011) . 【Conclusions】 There are obvious cerebral hemodynamic changes during NREM in ESES, and IEDs frequency are the important factors of cognitive impairment due to ESES. ESES pattern is closely related to MCBFV oscillations during deep sleep.

OBJECTIVE: To study the clinical features and recurrence factors of myelin oligodendrocyte glycoprotein (MOG) antibody disease in children and the effect of recurrence prevention regimens. METHODS: A retrospective analysis was performed on the medical data of 41 children with MOG antibody disease who were hospitalized in the Department of Pediatric Neurology, Xiangya Hospital of Central South University, from December 2014 to September 2020. According to the presence or absence of recurrence, they were divided into a monophasic course group ( RESULTS: For these 41 children, acute disseminated encephalomyelitis was the most common initial manifestation and was observed in 23 children (56%). Of the 41 children, 22 (54%) experienced recurrence, with 57 recurrence events in total, among which optic neuritis was the most common event (17/57, 30%). The proportion of children in the recurrence group who were treated with corticosteroids for less than 3 months in the acute phase was higher than that in the monophasic course group (64% CONCLUSIONS More than half of the children with MOG antibody disease may experience recurrence. Most children with recurrence are treated with corticosteroids for less than 3 months in the acute phase. Rituximab and azathioprine may reduce the risk of recurrence.
Autoantibodies ; Child ; Humans ; Myelin-Oligodendrocyte Glycoprotein ; Optic Neuritis ; Recurrence ; Retrospective Studies

Objective    To explore the mechanism of DDX46 regulation of esophageal squamous cell carcinoma. Methods    Picture signals of fluorescence in gene array were scanned and differential expression of gene in two groups (a DDX46-shRNA-LV group and a control-LV group) were compared by GCOSvL.4 software. These differential expressed genes were analyzed by bioinformatics methods finally, and validated by quantitative real time polymerase chain reaction (qRT-PCR) analysis. Results    According to the screening criteria of fold change ≥2 and P<0.05, 1 006 genes were differentially expressed after DDX46 knockdown, including 362 up-regulated and 644 down-regulated genes. Bioinformatics analysis and gene co-expression network building identified that these differentially expressed genes were mainly involved in cell cycle, proliferation, apoptosis, adhesion, energy metabolism, immune response, etc. Phosphatidylinositol 3-kinase (PI3K) was the key molecule in the network. The results of RT-qPCR were completely consistent with the results of gene microarra. Conclusion    Bioinformatics can effectively exploit the microarray data of esophageal squamous cell carcinoma after DDX46 knockdown, which provides a valuable clue for further exploration of DDX46 tumorigenesis mechanism and helps to find potential drug therapy.