Objective To understand on factors affecting adult -educated undergraduates' psychological health situation. Methods One hundred and ninety adult-educated undergraduates of a university were selected in random to let them take tests of SCL-90 measuring table a nd EPQ questionaire. Results There were 44.44 percent of adult-educated undergraduates who had psychological obstacle. Important affecting variables were screened out. Conclusions After knowing these important affecting variables, we can tak e some actions to reduce the occurence of psychological obstacle among adult-ed ucated undergraduates.

Objective To develop a kind of clinical nursing human resource mamagement basic data set fits to national and international standard. Methods CNHRMBDS was developed according to refereces and related standard, and then compared with related data set of nursing management. Results There were 179 data elements in the CNHRMBDS. It included all data elements related to human resources for health in China Hospital Basic Data Set Standard 1.0Version, China Public Health Information Classification and Basic Data Set Standard1.0 Version, and Community Health Information Basic Data Set Standard. Twenty-four data elements in the World Health Organization Human Resources for Health Minimum Data Set and 155 data elements in the American Nursing Management Minimum Data Set were found comparable to those in the CNHRMBDS. Conclusions The CNHRMBDS was found complete and able to meet relevant national and in-ternational standards. It should be further developed to be a national standard for health profession.

Objective To investigate the relaxant effect of salanic acid A isopropyl ester(isopropyl 3-(3,4-dihydroxyphenyl)-2-hydroxypropanoate)and its mechanism.Methods Isolated rat pulmonary artery was perfused and the tension of the vessel was measured.To observe the relaxant effect of salianic acid A isopropyl ester on the pulmonary artery precontracted by noradrenaline(NE)and the role of endothelial cell and vascular smooth muscle cell on vasodilation.Results Salianic acid A isopropyl ester relaxed the endothelium-intact artery precontracted by NE in a concentration-dependent manner,and the effect was depressed with denuded endothelium.Salianic acid A isopropyl ester right-shifted the concentration-response curve of KCl and suppressed the contractions induced by NE and CaCl2 in the Ca2+-free-K-H solution.The relaxant effect of salianic acid A isopropyl ester was inhibited by N?-nitro-L-arginine methyl ester hydrochloride(L-NAME),Indometacin,CsCl,Tetraethylammonium(TEA),Glibenclamide,and BaCl2,but not that by Propranolol,Metoprolol,and Salbutamol.Conclusion Salranic acid A isopropyl ester could significantly relax the pulmonary artery of rats,which may derive in part from endothelium.The relaxant effect of salianic acid A ispropyl ester may relate to NO pathway,prostacyclin(PGI2)pathway,calcium channel,and potassium channel.

Objective To investigate the value of Computed Tomography Myelography(CTM) and CT in diagnosis of in intravertebral tumors.Methods 35 tumors in the canales spinalis examined by CTM and CT and confirmed by surgical-pathologic results were retrospectively analyzed.Results 10 intramedullary(28 6%),15 intradural extramedullary(42 9%) ,8 intradural(22 9%) and 2 intra-extradural tumors(5 7%) were diagnosed respectively by CTM and CT.Conclusion CTM and plain and enhancement CT scanning can reveal the features of every kind of tumors in the canales spinalis and determine correctly respective position and nature.

Objective To evaluate the safety and feasibility of transradial approach for coronary angiography.Methods Five hundred and ten cases of suspected coronary artery disease received coronary angiography by transradial approach.Results The procedures were successfully performed in 503 cases. Seven failed, of them, three failed in acupuncture, 1 had deformed radial artery,2 were with severe tortuosity of nonname artery and one case was due to left subclavian artery occlusion. The radial artery was occluded in one case after operation.There was no other severe complication. Conclusions Transradial approach for coronary angiography is safe and feasible.Its advantage lies in higher success rate and less complication.

Objective To investigate the efficacy and safety of intravenous diltiazem in patients with perioperative angina pectoris in coronary artery revascularization.Methods Totally 128 patients with angina pectoris during perioperation of coronary artery revascularization received intravenous diltiazem.The efficacy was determined by angina symptom,changes of blood pressure (BP) and heart rate (HR),and acute heart events after 2 weeks.Results Ninety-one cases had no relapse and 37 were obviously improved of angina symptoms.BP and HR were safely lowered after intravenous diltiazem.Of the 128 cases,85 received successful PCI and were discharged 1-3 weeks postoperatively;41 of 43 cases successfully received CABG;2 died of ventricular fibrillation and heart failure.There was not side-effect in all patients.Conclusion Intravenous diltiazem is effective and safe for with angina pectoris during perioperation of coronary artery revascularization.

OBJECTIVE: To present the design scheme of up-front outpatient prescription review system based on C/S (Client/Server) & B/S (Browser/Server) mixed-architecture in our hospital. METHODS: The core-technology and implementation procedure of up-front outpatient prescription review system were elaborated at length from the aspects of overall design, system developing environment,system function modules,realization of individualized review indexes, etc. At the same time, interrupted time series(ITS) analysis was used to analyze the changes in qualification rate of outpatient prescriptions. RESULTS: The implementation of up-front outpatient prescription review system reduced the rate of unqualified prescriptions effectively. During the implementation period, a total of 501 986 prescriptions were examined, and 31 161 prescriptions were successfully intervened. The qualification rate of prescription increased significantly. CONCLUSION: Up-front prescription review/intervention system is successfully developed in our hospital and is deserved to be generalized to promote rational use of drugs.

BACKGROUND: Stem cell transplantation has a significant neuroprotective effect on neurological diseases. Current transplantation methods such as arteriovenous transplantation and brain stereotactic transplantation are not suitable for clinical application in preterm infants. OBJECTIVE: To explore the feasibility of nasal transplantation of human umbilical cord mesenchymal stem cells and human neural stem cells for the treatment of white matter injury in premature rat infants. METHODS: Human umbilical cord mesenchymal stem cells were prepared from human umbilical cord tissue, and human neural stem cells were prepared from human embryonic brain tissue. In vitro migration of two kinds of cells was assessed by Transwell method. Forty 3-day-old Sprague-Dawley rats were randomly divided into sham operation group, model control group, human umbilical cord mesenchymal stem cell transplantation group and human neural stem cell transplantation group, with 10 rats in each group. Rats in all groups except the sham operation group were treated with right common carotid artery ligation and hypoxia for 90 minutes to establish a rat model of white matter injury in the preterm infant. Totally 1×106 cells were delivered intranasally in the transplantation group at 3 days after injury. Each nostril was infused with 5×105, and each nostril was infused once. On day 7 after injury, MBP immunofluorescence staining was used to detect the expression of myelin basic protein in the white matter of the brain to identify the damage of the white matter injury model. At 24 hours after transplantation, human umbilical cord mesenchymal stem cell migration was detected by anti-HuNu immunohistochemical method and human neural stem cell migration was detected by CM-Dil fluorescent labeling method. RESULTS AND CONCLUSION: (1) On day 7 after modeling, compared with the normal side, the positive area of MBP decreased in cingulate band, corpus callosum and external capsule of the affected side in the model of brain white matter injury in preterm infants (P < 0.05), indicating a successful modeling. (2) In vitro experiments showed that the migration rate of human neural stem cells was the same as that of human umbilical cord mesenchymal stem cells. (3) At 24 hours after the nasal transplantation, human umbilical cord mesenchymal stem cells migrated to the cortex, corpus callosum and hippocampus on the normal side and the damaged side, and human neural stem cells migrated to the damaged cortex, corpus callosum and hippocampus, and human umbilical cord mesenchymal stem cells migrated more than human neural stem cells. (4) Overall, these findings indicate that 24 hours after the nasal transplantation, human umbilical cord mesenchymal stem cells could survive and migrate to the normal side and the injury side, and human neural stem cells could survive and migrate to the injury side; and the migration of human umbilical cord mesenchymal stem cells was more extensive than that of human neural stem cells.

Objective To evaluate the effect of emulsified isoflurane on cognitive function in rats.Methods Seventy-two adult male SD rats,aged 8 weeks,weighing 250-300 g,were randomly divided into 3 groups:normal control group (group C,n =12),intralipid group(group E,n =12),and 8％ emulsified isoflurane group ( group EI,n =48).Morris water maze test was performed at 2 h after administration in group E and at 2 h,1,7,14 d after administration in 12 rats at each time point in group EI.The escape latency,staying time at the original platform quadrant,frequency of crossing the original platform and swimming speed were recorded.Orbital blood samples were taken from 6 rats in each group after water maze test for determination of the plasma corticosterone concentration,and then the animals were sacrificed and their hippocampi were removed for determination of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) contents.Brains were removed from another 6 rats in each group after water maze test for determination of the expression of BDNF and NGF in DG,CA3,CA2,CA1 of hippocampus.Results Compared with group C,in group EI the escape latency at 2 h after administration was prolonged,staying time at the original platform quadrant was shortened,the expression of BDNF in DG and CA3 of hippocampus was down-regulated and the BDNF content in hippocampus was decreased at 2 h and 1 d after administration( P ＜ 0.05 or 0.01).The escape latency was shortened and staying time at the original platform quadrant was prolonged at 7 and 14 d after administration,the content of NGF in hippocampus was increased at 1,7 and 14 d after administration and the expression of BDNF in DG and CA3 of hippocampus was up-regulated at 1d after administration as compared with those at 2 h after administration in group E1( P ＜ 0.05 or 0.01).There was no significant difference in the variables mentioned above between groups E and C( P ＞ 0.05 ).There was no significant difference in plasma corticosterone concentration among the 3 groups ( P ＞ 0.01 ).Conclusion The mechanism by which emulsified isoflurane results in transient cognitive impairment in rats is related to down-regulating the expression of BDNF in hippocampus,but not related to corticosterone and NGF.

Objective To provide the basis for early detection and treatment of precancerous lesions of gastric carcinoma, and reference for early discovery, diagnoses and treatment of gastric cancer, by examining the differences of expression of Helicobaeter pylori (Hp), both in the gastric mueosa and in blood serum, between precancerous lesions of gastric carcinoma patients and gastric cancer patients. Methods Used immunechromatographic method and protein chip system to determine the expression of Hp in blood serum and used immunohistochemistry to detect the expression of Hp in gastric mucosa. The patients had been diagnosed by pathological methods, 170 patients with precancerous lesions of gastric carcinoma, 111 patients with gastric carcinoma and 31 patients with superficial gastritis. Results The expression of Hp was detected on every stage of precancerous lesions and gastric carcinoma. Compared with the detection rates of Hp, precancerous lesions of gastric carcinoma was higher than gastric carcinoma and superficial gastritis, superficial gastritis was the lowest. The differences of the three were significant (P<0.01). The detection rate of Hp in the gastric mueosa (86.2%, 66.7%, 38.7%, respectively)was obviously higher than in blood serum (80.6%, 52.3%, 32.3%, respectively). Conclusions Hp participates in the process of the precancerous lesions and gastric carcinoma. The expression of Hp both in the gastric mueosa and in the blood serum could be one of the indexes, which might report the process and curative effect of precancerous lesions. With such advantages as simple and applicability, the detection of Hp in the blood serum could be used widely. The endoscopy and the detection of Hp in the gastric mucosa are suggested for patients with Hp positive in the blood serum.