Adult virgin female mice were killed at each stage of the estrous cycle. The mid pieces of uteri were taken and fixed in 4% neutral formaldehyde, and stained with 0.5% toluidine blue at pH 0.5. It was found that mast cells in uterus were mainly distributed in the myometrium, only a few in the endometrium. The mast cell number was maximum in metestrus and minimum in proestrus, and this difference was highly significant (P

Objective To investigate the spatiotemporal expression patterns and the relationship of ?-sarcomeric actin(?-SCA),?-smooth muscle actin(?-SMA) and intermediate filament protein desmin with the maturation of the prenatal and the neonatal mouse hearts.Methods Serial sections of the embryo mouse and the neonatal mouse hearts were immunostained with antibodies against ?-SCA,?-SMA and desmin.Results Ventricle and outflow tract of embryonic day(ED) 9 heart showed stronger expression of ?-SCA and ?-SMA,but desmin expression was lower.In the atrium,the expressions of ?-SCA and ?-SMA were restricted to the dorsal and ventral walls.In the sinus venosus,only a few weakly stained ?-SCA positive cells were detected.No desmin expression was found in the atrium and sinus venosus.The expressions of ?-SCA,?-SMA and desmin were increased to their highest level at ED 12.The higher expression of ?-SCA remained to the postnatal stages.After ED 12,the expressions of ?-SMA and desmin gradually decreased in different parts of the heart,but their expressions in the right ventricle persisted longer.After birth,desmin expression was mainly concentrated in the Z lines of I bands and intercalated disks.Conclusion The presence of spatiotemporal differences in the expression of ?-SMA and desmin reveals regional differences in cardiomyocyte maturation in various parts of the embryonic mouse heart.The right ventricle shows a relatively slow pace of maturation.The ?-SMA may contribute to a peristaltoid contraction pattern of the embryonic myocardium with a slow shortening speed,and a relatively higher level of desmin is required for the maturation of the sarcomere.

Objective: To transfuse rhesus bone marrow derived mesenchymal stem cells (MSCs) to rhesus leucocytes antigen (RhLA) mismatched recipients, in attempt to study the immunological reaction between recipients' T lymphocytes and the skin of donor or a third party. Methods: Bone marrow aspirates were collected from femurs of donor rhesus, and the mononuclear cells were isolated and resuspended in low glucose Dulbecco's Modified Eagles Medium (DMEM) containing 2% fetal bovine serum. Phenotype of MSCs was analyzed by flow cytometry and the differentiated cells were identified by relevant specific staining. Allogeneic MSCs were given to recipients (single infusion) without preconditioning. Peripheral blood lymphocytes from recipients were co-cultured in vitro with the donor's skin or a third rhesus for 72 h before and 20 days, 40 days, and 60 days after infusion. The pathological lesions were evaluated and the keratinocyte apoptosis of the skin sections was determined by TUNEL assay. Results: Twenty days after infusion, the pathological lesion in in vitro skin explants from MSCs donor was less and the apoptosis rate was declined to (12.2±1.9)% from a pre-infusion level of (23.0±4.9)%. The apoptosis of cells in skin explants from the third party also declined to (11.8±1.4)% from a pre-infusion level of (22.0±2.0)% (P<0.05). Intensive immunological reaction was observed between recipient and donor/the third party on day 40 and day 60 after transplantation, with the apoptosis rate being (22.0±3.2)%, (22.0±2.5)% (donor) and (23.2±2.4)%, (24.0±2.6)% (the third party). Conclusion: Allogenic MSCs transfusion can decrease the immunological reaction between donors and recipients in rhesus, but this decreased reaction is temporary and reversible.

Humans ; Leukemia, Myelogenous, Chronic, BCR-ABL Positive ; metabolism ; Proto-Oncogene Proteins c-akt ; metabolism ; Signal Transduction

Adult ; Anemia, Aplastic ; complications ; surgery ; Female ; Hematopoietic Stem Cell Transplantation ; Hemoglobinuria, Paroxysmal ; complications ; surgery ; Humans

Cause of Death ; Female ; Hospital Mortality ; Hospitalization ; statistics & numerical data ; Humans ; Infant, Newborn ; Inpatients ; statistics & numerical data ; Male

Objective To compare and analyze the transcriptome of rhizome and leaves of Dioscorea zingiberensis, and excavate the key enzyme genes related to the saponin biosynthetic pathway in D. zingiberensis. Methods The transcriptome of rhizome and leaves of D. zingiberensis were sequenced by Illumina HiSeq2000 high-throughput sequencing technique. According to sequence annotate results to find the differentially expressed genes. Then the key enzyme genes related to the biosynthesis of diosgenin were identified according to the content of saponins in rhizomes and leaves of D. zingiberensis. The expression levels of some candidate genes were analyzed by real-time fluorescence quantitative PCR (qRT-PCR). Results A total of 81 660 Unigenes were gained and 64.33% of them were annotated in NT, NR, Swiss-Prot, KOG, GO, and KEGG databases. Based on their expression and KEGG annotation, totally 227 catalytic enzyme genes of 29 kinds that may participate in D. zingiberensis saponin biosynthetic pathway were screened. The expression pattern of some catalytic enzymes was correlated with the content of saponin. Also were found five D. zingiberensis endophyte genes. Conclusion This experiment obtained candidate key enzyme genes tentatively that involved in the biosynthesis of saponin. Some candidate enzyme genes may participate in the post-modification process of steroidal saponins in D. zingiberensis. In additon, it was found that D. zingiberensis endotrophic bacteria may be involved in the saponin biosynthesis. The results laid a foundation to further elucidate the molecular mechanism of sapogenin synthesis pathway.

ObjectiveTo explore the effects of extended nursing intervention (ENI) in nursing ability of discharged patients with intubation of T-tubes.Methods Eighty-two patients with biliary diseases discharged with intubation of T-tubes were equally divided into two groups.The patients in the experiment group were treated with ENI and those in the control group were treated with conventional nursing.Each group received routine nursing and health education before discharge.All patients were followed up by a self-designed written questionnaire.Data regarding patients’ compliance behavior,knowledge of the diseases and satisfaction were collected and analyzed.Result Significant differences were observed between the two groups,the patients in the experiment group exhibiting better compliance behavior,their knowledge of their diseases improved and the rate of satisfaction higher (P<0.05). Conclusion ENI may be effective for the discharged patients with intubation of T-tubes to improve their compliance behavior, enhance their knowledge of their diseases and raise the rate of satisfaction.

Objective To study pathogensand diagnostic value of beta2-microglobulin(?2-MG) in cerebrospinal fluid(CSF) in children with aseptic meningitis(AM).Methods Polymerase chain reaction (PCR) or reverse transcription-PCR (RT-PCR) assay,enzyme linked immunosorbent assay (ELISA) and passive particle agglutination were employed respectively to detect herpes simplex virus type-1(HSV-1) DNA,enterovirus(EV) RNA in CSF and the specific antibodies of HSV-1 and mycoplasma pneumonia(MP) in blood and CSF from 80 children with AM (AM group) and 20 cases of complete remission acute leukaemia(control group).Children with AM were divided into the EV meningitis group,HSV-1 meningitis group and MP meningitis group according to respective diagnostic criteria.The levels of ?2-MG in CSF from all groups were detected by radio immunoassay.SPSS 13.0 software was used to analyze the data.Results The infection rate of EV,HSV-1 and MP were 52.5%,11.25%and 5.0%,respectively in children with AM.The infection rate of EV was highest.The sensitivity of PCR was higher than ELISA.There could be false negative PCR results in bloody CSF specimens with HSV-1 DNA.The levels of ?2-MG in CSF was significantly higher in children with AM than that in control group(P

Congresses as Topic ; Humans ; Sleep Apnea, Obstructive