Objective To explore clinical characteristics and treatment of the thrombotic microangiopathy (TMA) induced by malignant hypertension, and provide a better understanding of the disease. Methods The clinical manifestations, laboratory examinations, therapeutic methods and prognosis of 15 TMA patients induced by malignant hypertension were analyzed retrospectively. Results The clinical manifestations were characterized by microangiopathic hemolytic anemia, thrombocytopenia and kidney injury. None of the patients required plasma exchange therapy. After antihypertensive treatment, all of 15 patients were discharged with improved clinical condition, remoglobin levels and platelet count. Conclusions The TMA induced by malignant hypertension is different from the classic TMA. Improving the understanding of TMA induced by malignant hypertension will contribute to early diagnosis and targeted treatment.

In 54 cases of pulmonary hypertension, the PaCO2 was markedly lowered, PaO2 and SaO2 elevated, HR, CO and mPAP, PVR all lowered in the treatment group (P0. 05), but SBP was significantly different (P

0.05).It was revealed by ROC curve analysis that P-LAP≤38.12 U/L and IL-6≥3.40 pg/mL could be adopted as criteria to predict the inevitable preterm labor,and the Youden's index of the combination use of the parameters of P-LAP and IL-6 was significantly higher than that of the single use of each parameter(P

AIM: To study the antiproliferation of vitamin E succinate (VES) on pterygium fibroblasts in vitro and to find a potential agent for prevention and treatment of primary and recurrence pterygium.METHODS: Primary culture and subculture of pterygium fibroblasts were established in vitro ,and different concentrations of VES (0, 10 and 20mg/L) were added to subcultured fibroblasts, respectively. Influence of VES on the growth curve of fibroblast was observed at day 2, 4 and 7 after treatment of VES. 3- [4,5-Dimethylthiazolzyl]-2,5-Diphenyl Tetrazolium Bromide (MTT) assay at 490nm was used to evaluate the effect of the cells proliferation.RESULTS: The addition of VES to culture caused the marked descent of growth curve in comparison with the control group, and the inhibiting rate of 10 and 20mg/L of VES was 33.2% and 46.7%, 67.9%, and 76.8%, 81.7% at day 2,4 and 7, respectively. VES could obviously inhibit the fibroblast proliferation in dose-dependent manner by MTT assay.CONCLUSION: VES can significantly inhibit the proliferation of pterygium fibroblast in vitro.

OBJECTIVE: To analyze the relationship between hygroscopicity and water solubility of chemical drugs by using nonparametric test. METHODS: Dynamic vapor sorption (DVS) analysis was adopted to determine the hygroscopicity of 515 chemical drugs, and solubility test method was adopted to determine the water solubility of these drugs. All samples were divided into four levels according to their water solubility, and the relationship between hygroscopicity and water solubility was investigated by using Kruskal-Wallis&Wilcoxon rank sum test. RESULTS: There were significant differences in the hygroscopicity of drugs with different levels of water solubility, and the hygroscopicity of level 4 was significantly higher than that of level 1. Hygroscopicity was positively correlated with water solubility. CONCLUSION The hygroscopicity of chemical drugs is interpreted in terms of a physical property in this study, and the results provide an experimental foundation for further studies on the mechanism of hygroscopicity.

OBJECTIVE: To investigate the moisture sorption properties of chemical reference substances (CRSs) by using dynamic vapor sorption (DVS) analysis technology in order to establish the distribution conditions, packaging materials, and usages of national chemical reference substances. METHODS: DVS analysis was adopted to acquire the moisture sorption dynamic profiles of five chemical reference substances of different moisture sorption types to evaluate their moisture sorption trend and capacities under different humidities. RESULTS: According to moisture sorption dynamics, we can found the hygroscopicity of disodium etidronate, sodium aminosalicylate, valaciclovir hydrochloride, aspirin and bosentan, and we have given advices about how to use these CRSs and what kind of bottles they should be packed in. CONCLUSION: DVS analysis can be used to record moisture sorption data in real time and visually observe water-CRS interactions under different humidities, which is an important technology for guiding the establishment and distribution of CRSs.

OBJECTIVE: To establish the detection technology of riboflavin polymorphism, and investigate the moisture sorption properties of riboflavin. METHODS: The two crystal forms of riboflavin were characterized by different analysis methods, such as powder X-ray diffraction method (p-XRD), differential scanning calorimetry method (DSC) and infrared spectrum method (IR). Dynamic vapor sorption (DVS) analysis was adopted to acquire moisture sorption dynamics of the two crystal forms. The influences of temperature, humidity and light on crystal transformation were explored. RESULTS: The crystal forms of two batches of riboflavin samples were identified as anhydrate I and monohydrate, respectively. The monohydrate could be obtained by recrystallizing in DM-SO. The monohydrate was hygroscopic, the number of crystal water may change at different relative humidities, and the moisture sorption dynamics of the monohydrate was greatly influenced by temperature. The anhydrate I was almost non-hygroscopic, and temperature hardly affected its moisture sorption dynamics. CONCLUSION: Characteristic data for riboflavin polymorphism are obtained, and the results show that anhydrate I is more stable than monohydrate. This study indicates that DVS could be used in studies on drug polymorphism.

Objective:To construct a canine thoracic aortic dissection(TAD) model for later studying. Methods:The soft tube with graduation and the puncture needle were used to produce the appliance for making TAD model.Under X ray, the appliance was placed into the canine thoracic aortic for elastin enzyme perfusion through iliac arteries or abdominal aorta. Results:TAD were showed instantly,2 weeks and 1 month later by angiography. Conclusion:This appliance can construct a canine TAD model.

Humans ; Renal Artery Obstruction ; therapy ; Stents

BACKGROUND:At present, there is no relevant systematic research about the effects of ankle-foot orthoses on lower limb muscles. OBJECTIVE:To extract the main features of the surface electromyography signals of the lower limbs from normal people with ankle-foot orthoses and to analyze the effects of the fixed ankle-foot orthoses on lower limb muscle fatigue. METHODS:Each of five male healthy young adults was respectively required to walk with and without special ankle-foot orthoses for three times. (1) Group 1, walking in a nature gait without orthoses. (2) Group 2, walking in a natural gait with ankle-foot orthoses (the ankle center was packaged over 1.0 cm by the orthoses that was recorded 1.0 cm); wearing the same orthoses but 1.0 cm in the ankle was removed and the ankle plastic edge was just through the ankle center to colect the data (recorded as 0 cm); after 1.0 cm was removed again in the ankle, walking in a natural gait (recorded as-1.0 cm). (3) Group 3, wearing 1.5 cm heel-height ankle-foot orthoses and walking in a natural gait; wearing the same orthoses but with 1.0 cm and 2.0 cm heel-height and walking in a natural gait. During walking, surface electromyography signals were colected from the biceps femoris muscle, rectus femoris muscle, tibialis anterior muscle, and gastrocnemius of the lower limbs. RESULTS AND CONCLUSION: (1) Without orthoses, the surface electromyography signals were the weakest. (2) For any muscle, the electrical activity of subjects without orthoses was weaker than that of subjects wearing orthoses at different hardness. (3) For the biceps femoris muscle, the integral electrical value without orthoses was very close to that wearing orthoses with normal heel height. Meanwhile, these two values were lower than that wearing orthoses with abnormal heel height. These findings indicate that the fixed plastic ankle-foot orthoses could cause the fatigue of the biceps femoris muscle, rectus femoris muscle, tibialis anterior muscle, and gastrocnemius; and the orthoses with abnormal heel height can further increase the fatigue of the biceps femoris muscle.