Objective:To explore the isolation,culture,proliferation and fluor-labelling of the fetal bone mesenchymal stem cell (BMSCs),and their biological character. Methods:Limb bone marrow samples from fetus aged 4 to 5 months were obtained, BMSCs were isolated by Ficoll centrifugalization and adherence screening method,and then cultured,proliferated to the 12th generation in vitro. The growth curve,cell cycle,phenotype,enzymatic activity,glycogen and the DAPI fluor-labelling of BMSCs were observed. Results:(1)Major BMSCs were fusiform, arranged as whirlpool. After the 12th generation,BMSCs presented the signs of senility. The subcultured cells proliferated faster than primary cells. (2)The 3rd and 8th generation of BMSCs expressed CD29,D71,but not CD34,HLA-DR;the G0,G1 period cells were 93.73%,and the S,M,G2 period cells were 6.27%. (3)The 5th generation cells were positive of nonspecific esterase and glycogen,but weak positive of alkaline phosphatase. (4)DAPI labeled BMSCs grew normaly,and the marking rate of BMSCs was 100%,but decreased gradually,and only 15% cells were labbelled for the 3th week. Conclusion:The acquired fetal BMSCs have the character of MSCs. The DAPI labeling is a modus operandi for the short period tracing BMSCs.

Objective To establish HPLC method for determination of the related substances in gatifloxacin mesylate for injection.Methods Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).All the chief peaks of gatifloxacin and those of their related substances were well isolated in all the specificity tests.the detective limit was 0.038?g/ml(S/N=4).the total peak area of therelated substances of all batches of samples was not more 1% of the peak area of their solutions.Conclusions This method is simple,accurate and stable.

Objective To establish the method for the determination of gatifloxacin mesylate for injection.Methods The chromatographic condition was as follows:Determination was performed with C18(150mm?4.6mm,5?m)as the chromatographic column.The mobile phase was consisted of phosphoric acid-acetonitrile(82:18)with a flow rate at 1.0 ml/min.The column temperature was 30℃ and the detection wavelength was 325nm.Results Good linear relation was achieved when the gatifloxacin concentration was at the range of 78.8～630.4?g/ml(r=0.9999).At the concentration of 80% 、100%、120%,the mean recovery were 99.45%(RSD 1.29%,n=3)、99.24%(RSD 0.47%,n=3)、99.81%(RSD 0.75%,n=3).,respectively.Conclusion The method is accurate with a good reproducibility and can be used as a quantitative analys for preparation.

Animals ; Humans ; Phytotherapy ; Rheumatic Diseases ; drug therapy ; Stilbenes ; therapeutic use

BACKGROUND: BK viral infection after renal transplantation influences the prognosis of BK virus-associated nephropathy in renal transplant recipients. The disease has been widely studied in foreign countries.OBJECTIVE: This study was designed to sum up the diagnosis and treatment of BK virus-associated nephropathy after renal transplantation.RETRIVAL STRATEGY: Using the terms "renal transplantation; BK virus" in the English language, manuscripts responsible for BK virus-associated nephropathy after renal transplantation that were published from January 2000 to January 2007 were retrieved from the PubMed database. A total of 206 manuscripts were obtained and primarily screened. Inclusion criteria: studies addressing BK virus-associated nephropathy after renal transplantation. Exclusion criteria: repetitive studies.LITERATURE EVALUATION: The included manuscripts were primarily from PubMed database. Manuscripts were primarily original and review studies.DATA SYNTHESIS: BK virus can be found in the urine of 3%-40% of adult renal transplant recipients. BK virus reactivation rate is very high, but the histological manifestations of BK virus associated nephropathy are found only in a small number of renal transplant recipients. The prognosis of BK virus associated nephropathy is very poor. BK virus associated nephropathy develops into renal failure, leading to transplant loss, in 30%-50% patients. BK virus-caused renal transplant disease must be diagnosed according to histological manifestations. Viral infection should be primarily confirmed, but serological measurements have no predominant effects. Electron microscopy should be involved in the assessment of renal graft biopsy, especially when renal failure factors are unknown, as through the use of electron microscope, viral particles in the nucleus, cytoplasm and outside of the cells could be detectable. Viral antigen in the urine sample of patients with BK viruria can be detected by nucleic acid hybridization method, immunofluorescence, and ELISA. Cidofovir is an effective medicine for treatment of BK virus-associated nephropathy, but its potential nephrotoxicity, proper dose, pharmacokinetics, safety, tolerance, anti-BK viral activities have not been confirmed. So fresher study should be required for aforementioned uncertainties.CONCLUSION: BK virus-associated nephropathy has poor prognosis and should be diagnosed according to histological examinations. BK virus-associated nephropathy has been treated primarily by symptomatic supportive treatment and reducing the dose of immunosuppressive agents.

Objective To observe the clinical efficacy of Tong Du Wen Yang (unblocking the Governor Vessel and warming yang) in treating amyotrophic lateral sclerosis (ALS).Method Twenty-eight eligible ALS patients were randomized into two groups by following the visiting sequence. Thirteen patients in the control group were intervened by orally taking Riluzole tablets; 15 cases in the treatment group were by Tong Du Wen Yang needling in addition to oral administration of Riluzole tablets. Before treatment and after 6-month treatment, the therapeutic efficacies were evaluated by using the Chinese medicine syndrome and sign scoring for Wei-flaccidity diseases and Appel function scale.Result After the treatment, the Chinese syndrome and sign scoring scores for Wei-flaccidity dropped in both groups, and the decrease in the treatment group was more significant than that in the control group (P<0.05). The Appel scores dropped in both groups after the treatment, while the decrease in the treatment group was less significant than that in the control group (P<0.05).Conclusion Tong Du Wen Yang needling plus orally taking Riluzole tablets can produce a more significant efficacy than using Western medication alone in treating ALS.

Nutrition support therapy plays an important role in the treatment of severe acute pancreatitis (SAP).This article reviews the hot topics and new ideas in nutrition support therapy for SAP in recent years.Damage of intestinal mucosal barrier plays an important role in the progression of pancreatitis.Enteral nutrition can protect the function of intestinal mucosal barrier and early enteral nutrition can significantly reduce patients′mortality rate and incidence rate of complications.Nutrition support for SAP patients via a nasogastric tube has comparable safety and effect to the nutrition support via a nasojejunal tube.At present,there are no enough experience and clinical studies on semi -el-emental or elemental enteral nutrition preparations,and immune -enhancing enteral nutrition preparations and probiotics are not recommen-ded as conventional means.

OBJECTIVE:To observe the clinical efficacy and safety of the effect of desloratadine citrate disodium in the decre-mental treatment of chronic urticaria. METHODS:212 patients with chronic urticaria were randomly divided into decremental treat-ment group and control group. Decremental treatment group was treated with Desloratadine citrate disodium capsules 17.6 mg in the first week,orally,qd;8.8 mg in the second week,orally,qd;8.8 mg in the third week,orally,qod;and 8.8 mg in the fourth week,orally,q3d. Control group was treated with Desloratadine citrate disodium capsules 8.8 mg,orally,qd. The treatment course for both groups was 4 weeks. The clinic data was observed,including clinical efficacy,and serum IgE,clinical symptom scores before and after treatment,recurrence rate and incidence of adverse reactions. RESULTS:The differences were not statistical-ly significant in the total effective rate and incidence of adverse reactions between 2 groups(P>0.05). The recurrence rate in decre-mental treatment was significantly lower than control group(P<0.05). When treatment ended and after 4 weeks of drug withdraw-al,serum IgE levels in 2 groups were significantly lower than before,and decremental treatment group was lower than control group(P<0.01);the IgE level in control group was signiciantly higher after 4 weeks of drug withdrawal(P<0.01)than the end of treatment. After treatment,clinical sympton scores in 2 groups were significantly lower than before(P<0.01),however the ditferenc-es was not significant between 2 groups(P>0.05). CONCLUSIONS:Desloratadine citrate disodium have similar clinical efficacies and safety in the decremental treatment and conventional treatment of chronic urticaria. However,decremental treatment of deslo-ratadine citrate disodium is better than conventional treatment in terms of improving the level of IgE.

BACKGROUND:Three-dimensional printing technique has been applied in medical fields since it was invented in the end of 20th century. Recently it has been widely used in manufacturing scaffolds for bone tissue engineering. OBJECTIVE:To review the basic concept of the scaffold for bone tissue engineering, the basic requirements for three-dimensional printing technique in scaffold engineering, different materials used in bone tissue engineering, the advantages and limitations of three-dimensional printing technique and the outlook of three-dimensional printing technique applied in manufacturing scaffold for bone tissue engineering. METHODS: The first author did a computer-aided retrieval of the MEDLINE database, Science Direct database, CNKI database, and CQVIP database for articles relevant to three-dimensional printing technique used in manufacturing scaffolds for bone tissue engineering published between January 1990 and February 2015. The key words were three-dimensional printing, tissue engineering, rapid prototyping technology, scaffold, materials in English and Chinese, respectively. Repetitive studies were excluded, and 33 of 52 related literatures were adopted in result analysis. RESULTS AND CONCLUSION: Three-dimensional printing technique has many advantages such as high resolution, high velocity and the freedom to build unlimited geometries. There are some requirements for the powder and binder used to construct bone tissue engineering scaffolds using the three-dimensional printing technique, such as the flowability, stability and wettability. A wide range of materials can be used: synthetic and natural polymers, ceramics, as wel as composites of the aforementioned. Various kinds of powder take responsibility of different features of scaffolds, resulting from the advantages and disadvantages of different materials. Although this technique has some limitations such as high cost and the difficulty of commercial production, its application stil has a bright future.

In order to further promote friendly cooperation with developing countries, Chinese government has been committed to increasing the intensity of cooperation and human resource development among developing countries. Healthcare is one of the key areas of China’s foreign aid. This article described the general conditions and experiences of national foreign aid training projects for developing countries undertaken by the Beijing Traditional Chinese Medicine Hospital and Beijing Traditional Chinese Medicine Research Institute. From aspects of participant enrollment, training program implementation, and training feedback, the training mode of national foreign aid on traditional medicine was introduced. This article focused on the selection of teachers, preparation of professional textbooks, and evaluation of teaching quality. Training programs over the years were guided by clear training concepts and accurate goal settings, which provided a useful exploration for the personnel training mode of national foreign aid on traditional medicine.