Objective To preliminary investigate and summarize the experiences of maintaining renal function and caring the cardio preoperative patients who have renal insufficiency (renal failure stage). Methods The treatment and cared for five cardio preoperative patients who had renal insufficiency were studied, those patients had been hospitalized in Peking University People′s Hospital for cardiac surgeries from June 2013 to January 2016. Results All the five patients had received preoperative renal maintenance, none of them got severe acute renal failure, and four of them had to get regular hem dialysis treatment after discharging. One of them died because of heart failure. Conclusions Renal insufficiency is a high risk for cardiac surgeries, however, it is not a contraindication for cardiac surgeries. Preoperative evaluation, preoperative caring and the application of CRRT can reduce the complications and improve the prognosis.

Lactic acid bacteria (LAB) are traditional dairy strains which have a long history of safe use. They are a kind of food-grade microorganisms that can be used in industry to produce lots of food and drink, such as yoghourt. In the last two decades the physiology and genetics of these bacteria have been thoroughly studied. Because of their genetic accessibility and easy to handle, LAB, in addition to their traditional applications, have been extensively developed and used for the expression of heterologous genes. So they have an important foreground in agricultural, medical and many other fields. People have developed a series of food-grade gene expression systems of LAB. LAB, especially their model strain Lactococcus lactis and their most useful food-grade induced expression system--nisin controlled gene expression system NICE and its food-grade inducer nisin, food-grade host, expressing vectors and applications in expressing heterologous proteins of NICE will be introduced.

Cardiovascular Diseases ; etiology ; Diet ; Glycemic Index ; Humans

No abstract available.

Abruptio Placentae ; complications ; Adult ; Disseminated Intravascular Coagulation ; diagnosis ; etiology ; therapy ; Female ; Humans ; Infant, Newborn ; Male ; Pregnancy ; Prognosis ; Risk Factors

China ; Clinical Trials as Topic ; methods ; Humans ; Organizational Policy ; Publishing ; standards ; Registries

Objective To investigate the effects of percutaneous coronary intervention(PCI) and conventional drugs on autonomic nervous system and C-reactive protein (CRP)in the patients with unstable angina pectoris combined by QRS complex fragment. Methods A total of 60 patients aged (46.2± 10.3) years with unstable angina combined by QRS complex fragment were randomly divided into drug therapy group and PCI group (n=30 for each group).The changes of heart rate variability (HRV) including SDNN,SDANN,rMSSD,PNN50,HF and LF,heart rate turbulence(HRT) such as TO and TS,and CRP were measured before and 1 month after treatment. Results The values of SDNN[(88.2±20.6)ms vs.(122.5 ± 15.5)ms; (86.9± 23.4)ms vs.( 106.7± 18.8)ms],SDANN [(76.2±9.3)ms vs.(105.3±5.2)ms; (74.3±10.4)ms vs.(89.8±7.6)ms],rMSSD[(18.6±7.9)ms vs.(49.3± 4.3)ms; (19.3± 7.4)ms vs.(29.4± 5.2)ms],PNN50 [(5.5± 2.8)％ vs.(9.1 ±1.8)％; (5.3±2.1)％ vs.(7.2±3.2)％],HF[(219.4±131.6) Hz vs.(292.5±125.5) Hz;(217.2±133.2) Hz vs.(213.2±120.2 ) Hz] and LF[(459.6±135.2) Hz vs.(345.1±175.1) Hz ;(445.8± 144.3) Hz vs.(396.1 ± 182.3) Hz] were improved after treatment as compared with pretreatment in PCI group (t=9.4,15.69,8.37,4.68,3.26,3.57,P＜0.01 or 0.05) and in drug therapy group (t=7.3,12.36,6.98,2.94,4.89,5.01,P＜0.01 or 0.05),respectively.The changes of above indexes were more remarkable in PCI group than in drug therapy group(t=8.90,13.75,7.52,3.27,4.21,4.01,P＜0.01 or 0.05).The values of turbulence onset(TO) [(0.45±0.44)％ vs.(0.16±0.20)％,t=15.63,P＜0.01; (0.49±0.38)％ vs.(0.32±0.26)％,t=16.78,P=0.001] and turbulence slope (TS) [(2.12±0.13)ms/RR vs.(2.98±0.25)ms/RR,t=14.36,P=0.001; (2.15±0.19)ms/RR vs.(2.51±0.11)ms/RR,t=19.52,P=0.001] and CRP [(5.74±2.46)mg/L vs.(2.61±1.22)mg/L,t=12.49,P=0.001; (5.81±2.35)mg/Lvs.(3.56±1.43)mg/L,t=9.76,P=0.01] were also improved after treatment as compared with before treatment in PCI and drug therapy groups,respectively,and the the changes in TO (t=15.95,P＜0.001),TS (t=18.13,P=0.001) and CRP(t=10.73,P=0.001) were more obvious in PCI group than in medicine group. Conclusions PCI may obviously reduce inflammatory response,provide more myocardial blood supply and improve autonomic nervous function in the patients with unstable angina pectoris combined by QRS complex fragment.

BACKGROUND: On the basis of traditional herb wuling san, tianqi and danshen are added to make a preparation, jianshen lishui I , for treating blood stasis and edema; shichangpu and xiakucao are added to make a preparation, xingnao xiaozhong capsule, for treating fire, sputum and qiaobi in cerebral edema.OBJECTIVE: To observe the differences between jianshen lishui Ⅰ and xingano xiaozhong capsule on treating vascular-derived cerebral edema.DESIGN: Randomized grouping design and blank controlled study.SETTING: Laboratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine.MATERIALS: The experiment was completed in the Lab.oratory of Neurology, Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine from March to April 2001. Totally 32 male rabbits with 7 months old were selected in the study.METHODS: ① Animal models with cerebral edema were established with self-fresh blood injection method. According to grouping method, animals were divided randomly into 4 groups: blank control group (without intervention); model group (without intervention after modeling); jianshen lishuiⅠ group (11.5 mL/kgjianshen lishui Ⅰ was perfused after modeling; jian-shen lishui Ⅰ consisted of 15 g sanqi, 15 g danshen, 10 gfuling, 10 gzhuling, 10 g zexie, 10 g baizhu and 10 g gu~hi, and provided by Dispensary of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine, 200 mL/bag) and xingnao xiaozhong capsule group [after modeling, 11.5 mL/kg xingnao xiaozhong suspension was made by 380 mg/kg boiled distilled water; certification: southern medicine (96) 05079; consisting of 15g xiakucao, 15 g shichangpu, 10 gfuling, 10 g zhuling, 10 g zexie, 10 g baizhu and 10 g guizhi, 13.33 g raw materials per capsule; provided by Agent Department of the Second Affiliated Hospital of Guangxi College of Traditional Chinese Medicine] with 8 in each group. Animals in each group were divided into 12 and 24 hours subgroups with 4 in each time points. ② Animals were killed 12 and 24 hours after intervention with air embolism to calculate cerebral coefficient (total brain mass/body mass × 100%), cerebral water content [(wet weight - dry weight)/wet weight × 100%], Evens blue content in brain tissue [absorbency (A value) × microgram in standard liquid/an A value × (25 mL/wet weight)]. ③Positivity was tested with square method; differences among groups were compared with F test; differences between groups were compared with q test; time points were tested with t test; and irregular variance was checked with rank sum test.MAIN OUTCOME MEASURES: Comparisons of cerebral coefficient,water content and content of Evans blue in 12 and 24-hour experiment group.RESULTS: Totally 32 rabbits entered the final analysis. ① 12-hour experiment: Water content and Evans blue content in jianshen lishui Ⅰgroup and xingnao xiaozhong capsule group were obviously lower than those in model group (P ＜ 0.01); cerebral coefficient in jianshen lishui I group was lower than that in xingnao xiaozhong capsule group, but the difference was not significant (P ＞ 0.05); water content and Evans blue content in jianshen lishui Ⅰ group were lower than those in xingnao xiaozhong capsule group (P ＜ 0.05). ② 24-hour experiment: Water content and Evans blue content in jianshen lishui Ⅰ group and xingnao xiaozhong capsule group were obviously lower than those in model group (P ＜ 0.01);cerebral coefficient in jianshen lishui Ⅰ group was lower than that in model group (P ＜ 0.01); cerebral coefficient, water content and Evans blue content in jianshen lishui Ⅰ group were respectively lower than those in xingnao xiaozhong capsule group (P ＜ 0.05).CONCLUSION: Vascular-derived edema has found 12 hours after modeling and cerebral edema deteriorates with time passing. In addition, effect of jianshen lishui Ⅰ on blood brain barrier is superior to that of xingnao xiaozhong capsule.

Objectives:To observe the influence of PN treatment on the postoperative patients of advanced ovarian cancer. Methods:The patients were divided into two groups.Thirty cases of patients(PN group) were treated with PN after the operation for the ovarian cancer.Thirty five cases of patients(control group) were treated regularly without PN.The biochemical indicators,complications and mortality rate were compared between the two groups. Results:The biochemical indicators in PN group were better than those in control group.The incidence of complications and mortality rate in PN group were significantly lower than those in control group. Conclusions:PN can improve the general status of postoperative patients with advanced ovarian cancer and decrease the complication incidence and motality rate.

OBJECTIVETo study the effects and underlying mechanisms of Zhuyun Recipe (ZR) on the endometrial receptivity in ovarian stimulation (OS) and blastocyst implantation dysfunction (BID) mice.

METHODSTotally 200 normal female Kunming mice were randomly divided into 6 groups, i. e., the control group (Group A), the OS group (Group B), the OS + ZR group (Group C), the BID group (Group D), the BID + ZR group (Group E), and the ZR group (Group F). The pregnant mare's serum gonadotrophin (PMSG) and human chorionic gonadotrophin (HCG) were intraperitoneally injected to mice in Group B. Mifepristone was subcutaneously injected to mice in Group D at 9:00 am on the 4th gestation day. Corresponding medications were given to mice in Group C, E, and F at 1.5 mL/100 g by gastrogavage at 8:00 am from the first to the 4th gestation day. Eight uterus samples were collected at 9:00 pm on the 4th gestation day and fixed. The expression levels of leukemia inhibitory factor (LIF) and integrin beta3 were detected using immunohistochemical assay. The pregnant mice were sacrificed at 9:30 pm on the 8th gestation day, and their uterus were taken out. The number of blastocysts was counted.

RESULTSCompared with Group A, the pregnant rate was 6.67% (1/15 cases) in Group B and 18.75% (3/16 cases) in Group D, the mean OD value of LIF was 0. 18 +/- 0.02 in Group B and 0.23 +/- 0.02 in Group D, and the mean OD value of integrin beta3 was 0.20 +/- 0.05 in Group B and 0.19 +/- 0. 02 in Group D, showing statistical difference (P < 0.01). The pregnant rate was 54.55% (12/22 cases) in Group C and 65. 22% (15/23 cases) in Group E, the mean OD value of LIF was 0.37 +/- 0. 09 in Group C and 0.39 +/- 0.02 in Group E, and the mean OD value of integrin beta3 was 0.34 +/- 0.04 in Group C and 0.38 +/- 0.08 in Group E, showing statistical difference when compared with those of Group B and Group D respectively (P < 0.05).

CONCLUSIONSOS and BID had negative effects on the endometrial receptivity and hindered the blastocyst implantation. ZR could improve the uterine receptivity and elevate the pregnant rate by up-regulating the expressions of endometrial LIF and integrin beta3.

Animals ; Drugs, Chinese Herbal ; pharmacology ; Embryo Implantation ; drug effects ; Endometrium ; drug effects ; physiology ; Female ; Integrin beta3 ; metabolism ; Leukemia Inhibitory Factor ; metabolism ; Mice ; Mice, Inbred Strains ; Ovulation Induction ; Pregnancy