1.Evaluation and cell warning information for clinical application of five-part differential hematology analyzer
Jing WANG ; Ming LI ; Li-Song SHEN ;
Chinese Journal of Laboratory Medicine 2003;0(12):-
Objective To investigate the technical charactenstics ot SYSMEX XS1000i 5-part differential automated hematological analyzer and its clinical applications.Methods 209 samples were analyzed with the analyzer of XS1000i and compared to the results from Beckman-Coulter LH750 analyzer.The main parameters from XS1000i,such as precision within-run,day to day precision and carryover contamination rates etc,were recorded respectively in 509 samples to compare the difference between the instrumental and optical examination.Results All variation coefficients of precision from XSI000i were within the manufacturer.The carryover contamination rates of WBC,RBC,Hb,HCT and PLT were 0.19%,0.93%,0,-0.88%,-0.76%,respectively.The parameters of XS1000i were correlated with the results of LH750 except basophil granulocyte.The correlation coefficients of WBC,RBC, HGB and PLT were 0.994 5,0.996 8,0.997 0 and 0.974 6 ,respectively.The sensitivity of warning flags in immature leukocytes was 100% and the specificity was 69.7%,the sensitivity of warning flags in atypical lymphocyte was 100% and the specificity was 66.7%.Especially in 3 leukocytopenia that was induced by chemotherapy in patients with leukemia who had only few immature cells existed in the peripheral blood,the parameters of XS1000i were positive,and correlated with the results of detection of minimal residual disease with flow cytometry.Conclusions The warning system of XSIO00i provides more valuable information for manual microscopic examination.If it is combined with flow cytometry,the advantages in detection of residual leukemic cell will be fully displayed.
2.Richter syndrome: report of a case.
Ding-bao CHEN ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2010;39(7):487-488
Aged
;
Antibodies, Monoclonal, Murine-Derived
;
therapeutic use
;
Antigens, CD20
;
metabolism
;
Antineoplastic Agents
;
therapeutic use
;
Antineoplastic Combined Chemotherapy Protocols
;
therapeutic use
;
CD5 Antigens
;
metabolism
;
Cyclophosphamide
;
therapeutic use
;
Doxorubicin
;
therapeutic use
;
Humans
;
Leukemia, Lymphocytic, Chronic, B-Cell
;
drug therapy
;
metabolism
;
pathology
;
Lymphoma, Large B-Cell, Diffuse
;
drug therapy
;
metabolism
;
pathology
;
Male
;
Prednisone
;
therapeutic use
;
Receptors, IgE
;
metabolism
;
Rituximab
;
Vincristine
;
therapeutic use
3.Adenocarcinoma of gallbladder with chondrosarcomatous component: report of a case.
Hong-fang ZHENG ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(12):770-770
Adenocarcinoma
;
metabolism
;
pathology
;
surgery
;
Aged
;
Cholecystectomy
;
Chondrosarcoma
;
metabolism
;
pathology
;
surgery
;
Female
;
Gallbladder
;
chemistry
;
pathology
;
surgery
;
Gallbladder Neoplasms
;
metabolism
;
pathology
;
surgery
;
Humans
;
Immunohistochemistry
;
Keratin-3
;
metabolism
;
S100 Proteins
;
metabolism
4.Nevus of Ota resistant to multiple sessions of Q-switched laser treatment: two case reports
Lingling HU ; Weimin SONG ; Jing SHEN ; Han ZHENG ; Junfan CHEN
Chinese Journal of Dermatology 2011;44(10):729-730
Two cases of nevus of Ota resistant to multiple sessions of Q-switched laser treatment are reported.Both of the patients were females,who developed beige or blue patches on the left side of the face (zygomatic region,cheek)several months after birth.No conjunctiva or sclera was involved.The lesions spread to the left retroauricular region and posterior neck during pregnancy in one patient.Histopathological examination of the lesions showed scattered melanocytes and melanophages in lower dermis and subcutaneous adipose tissue as well as apparent proliferation of collagen.Immunostaining for CD34 showed no abnormality in the distribution of vascular endothelial cells in these tissues.No obvious improvement was observed in either of the patient after more than 10 sessions(12 sessions in 1 case,and 13 sessions in the other case)of treatments with Q-switched alexandrite(755 nm)or Q-switched Nd:YAG laser(1064 nm).The immediate response of lesions to laser irradiation was unusual.Purpura or errhysis was absent in the lesions after repetitive irradiation with high-energy laser,but both were visible in the adjacent normal skin even after irradiation with low-energy laser.
6.Acupuncture for spastic hemiplegia after ischemic stroke:a systematic review
Fengjiao SHEN ; Diantao LUO ; Youhu SHEN ; Mingxia SONG ; Changshun HU ; Yuan ZHU ; Jing CHEN ; An MAO ; Bingye XIONG ; Hong XU
Journal of Acupuncture and Tuina Science 2024;22(6):503-513
Objective:To systematically assess the effectiveness and safety of acupuncture for spastic hemiplegia after ischemic stroke. Methods:Randomized controlled trials(RCTs)of acupuncture treatment for spastic hemiplegia after ischemic stroke meeting the inclusion criteria in Cochrane Library,Medline,Excerpta Medica Database(EMBASE),PubMed,China National Knowledge Infrastructure(CNKI),SinoMed,Chongqing VIP Database(VIP),and Wanfang Data Knowledge Service Platform(Wanfang)published from each database's inception to February 2023 were retrieved by computer.Two reviewers independently extracted data and evaluated the risk of bias using Cochrane's risk of bias tool.Review Manager 5.4 was used for data analysis.Continuous data were evaluated using mean difference(MD)with a 95%confidence interval(CI),and dichotomous data were analyzed using risk ratio(RR). Results:A total of 24 trials,including 1 970 participants,were included in the study.The meta-analysis of 7 trials showed that compared to the rehabilitation therapy,acupuncture therapy was more effective in improving the simplified Fugl-Meyer assessment score after 1-month treatments[MD=10.52,95%CI(7.81,13.23),P<0.001].The meta-analysis of 2 articles showed the same tendency after 6-month treatments[MD=19.18,95%CI(11.34,27.02),P<0.001],and the 6-month treatment course resulted in better outcomes than the 1-month course.The meta-analysis of 8 trials showed that acupuncture had a better improvement on the Barthel index score than rehabilitation therapy after 1-month treatments[MD=10.78,95%CI(8.91,12.64),P<0.001].The meta-analysis of 2 articles showed the same tendency after 6-month treatments[MD=19.94,95%CI(19.02,20.87),P<0.001],and the 6-month course was better than the 1-month course.The meta-analysis of 2 trials showed that the effective rate of the modified Ashworth scale score improvement was more notable in the acupuncture group after 1-month treatments[RR=1.20,95%CI(1.02,1.40),P=0.020].One trial reported no adverse event,and 1 trial reported 3 adverse events without severe influence. Conclusion:Acupuncture might be an effective and safe therapy for spastic hemiplegia after ischemic stroke,but more high-quality,large-sample objectively-evaluated RCTs are needed to validate the conclusion.
7.Triple staining of immunohistochemistry.
You-zhi YU ; Min LIN ; Wei-cheng XUE ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2005;34(4):244-245
8.Anaplastic large cell lymphoma of mixed sarcomatoid and giant-cell rich variant occurring in female external genitalia: report of a case.
Ding-bao CHEN ; Qiu-jing SONG ; Dong-mei BAO ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(12):759-760
Adult
;
Diagnosis, Differential
;
Female
;
Follow-Up Studies
;
Giant Cell Tumors
;
metabolism
;
pathology
;
surgery
;
Humans
;
Immunohistochemistry
;
Ki-1 Antigen
;
metabolism
;
Lymphoma, Large-Cell, Anaplastic
;
metabolism
;
pathology
;
surgery
;
Melanoma
;
pathology
;
Mucin-1
;
metabolism
;
Perineum
;
pathology
;
surgery
;
Protein-Tyrosine Kinases
;
metabolism
;
Receptor Protein-Tyrosine Kinases
;
Sarcoma
;
metabolism
;
pathology
;
surgery
9.Metastases of breast cancer to female genital tract: report of 2 cases.
Ding-bao CHEN ; Li-hua QIAN ; Qiu-jing SONG ; Dan-hua SHEN
Chinese Journal of Pathology 2006;35(3):190-191
Breast Neoplasms
;
metabolism
;
pathology
;
surgery
;
Carcinoma, Ductal, Breast
;
metabolism
;
secondary
;
surgery
;
Carrier Proteins
;
metabolism
;
Diagnosis, Differential
;
Endometrial Neoplasms
;
metabolism
;
secondary
;
Female
;
Glycoproteins
;
metabolism
;
Humans
;
Immunohistochemistry
;
Keratin-7
;
metabolism
;
Mastectomy, Modified Radical
;
Middle Aged
;
Ovarian Neoplasms
;
metabolism
;
secondary
10.Analysis of clinical features of 34 cases with liver cirrhosis complicated with chylous ascites
Kuiliang LIU ; Yuguang SUN ; Song XIA ; Wenbin SHEN ; Jing WU ; Xiangchun LIN
Chinese Journal of Digestion 2014;34(2):96-99
Objective To summarize the clinical characteristics of liver cirrhosis complicated with chylous ascites.Methods From July,2007 to November,2012,patients diagnosed as liver cirrhosis complicated with chylous ascites were retrospectively analyzed.Analyze the etiology,liver function,ascites,imaging examination,treatment options and progonsis of these patients.Differences in measurement data were compared with U test.Results A total of 34 cases were enrolled,male 27 cases and female seven cases,average age (51.7±12.5) years old.Hepatitis B (20/34,58.8%) was the most common etiology of liver cirrhosis.Child-Pugh grading was grade B (26 cases) and C (eight cases).The appearance of aseites of 17 cases was chylous and the serm-ascites albumin gradient (SAAG) was 18.8 (2.6 to 32.5) g/L.The SAAG of 27 cases (84.4%) was no less than 11.0 g/L.The ascites triglyceride (TG) level was 2.94 (0.26 to 16.75) mmol/L,the TG of 27 cases (84.4%) was no less than 1.25 mmol/L.The level of TG (2.66(0.26 to 16.75) mmol/L) of patients with SAAG over 11.0 g/L was significantly lower than that of SAAG lower than 11 g/L (7.07(2.26 to 15.67) mmol/L,U=24.00,P=0.02).The lymphatic scintigraphy indicated that imaging agent leaked into peritoneal cavity in 29 cases (85.3 %).Direct lymphangiography revealed lymphatic vessel structure abnormality in 15 cases (60 %).Twelve patients received conservative treatment and four patients were effective,13 patients accepted peritoneal-venous shunting and 12 patients were effective,11 patients with microsurgical treatrnent and seven patients were effective.Conclusions Lymphatic struture is a possible cause of cirrhosis patients with chylous ascites.The characteristics of chylous ascites in cirrhosis patients are still the same as the characteristics of portal hypertension ascites.The SAAG remarkably increases,and the the level of SAAG probably depends on TG level.Lymphoscintigraphy has great value on the determination of the presence of ascites.And the direct lymphangiography could help to reveal lymphatic vessel structure abnormality.