Objective: To observe the clinical curative effect of narrow band ultraviolet combined infrared with 8-Methoxypsoralen for the treatment of vitiligo,to seek a new method for the treatment of vitiligo. Methods: Sixty cases of vitiligo patients were randomly divided into experimental group and control group. The experimental group adopted 365-nm narrow band ultraviolet LED combined 850-nm infrared LED with 8-Methoxypsoralen for the treatment of vitiligo, the control group adopted 365-nm narrow band ultraviolet LED irradiation combined with 8-Methoxypsoralen for the treatment of vitiligo, the clinical curative effect of two group were observed when treated with 1 months,2 months. Results:After 2 months of treatment, the efficiency rate of the experimental group was 66.67%, higher than that of the control group with an efficiency of 40%, the difference was statistically significant(x2=4.653, P<0.05). Conclusion: The clinical efficacy of narrow band ultraviolet combined infrared with 8-Methoxypsoralen for the treatment of vitiligo is better than that of narrow band ultraviolet combined with 8-Methoxypsoralen in the treatment of vitiligo.

BACKGROUND:Aortic root homograft has been widely used, but it stil has a high incidence of calcification and degeneration after operation.
OBJECTIVE:To investigate the alteration of biological and immunological properties of rabbit aortic root homograft treated with de-endothelialization and decellularization methods, and to find a better decellularization method.
METHODS:Aortic root homografts obtained from rabbits were randomly divided into fresh group, de-endothelialization group, and decellularization group. The morphology of homografts was evaluated by hematoxylin-eosin, Masson and VG stainings. The biological properties such as thickness, tensile strength, broken extension rate, and heat shrinkage temperature were detected. The immunological features (inflammatory cellinfiltration, calcium content and CD152 expression) were determined with immunohistochemistry and flame atomic absorption spectrometry.
RESULTS AND CONCLUSION:As demonstrated in morphological observation, protocols of de-endothelialization and decellularization methods we used attained our desired aim of decellularization perfectly. Tensile strength and broken extension rate of decellularization group were decreased significantly compared with fresh group (P<0.01), while the difference between de-endothelialization group and fresh group was not significant (P>0.05). Hematoxylin-eosin staining showed that, inflammatory reaction was the most apparent in fresh group, showing a large amount of inflammatory cells infiltration;compared with fresh group, inflammatory reaction was significantly attenuated in de-endothelialization group, the number of inflammatory cells was significantly decreased. The inflammatory reaction was the lowest in the decellularization group, only inflammatory cells infiltrated the tissue edge. Immunohistochemistry showed that, CD152 expression was significantly increased in de-endothelialization and decellularization groups compared with fresh group (P<0.05). Calcium content in de-endothelialization and decellularization groups was significantly decreased compared with fresh group (P<0.05). It could be concluded that the immunogenicity of de-endothelialized and decellularized aortic homografts is decreased significantly, and the de-endothelialization method could better maintain the tensile property.

To analyze the composition and medication regularities of prescriptions treating hypochondriac pain in Chinese journal full-text database (CNKI) based on the traditional Chinese medicine inheritance support system, in order to provide a reference for further research and development for new traditional Chinese medicines treating hypochondriac pain. The traditional Chinese medicine inheritance support platform software V2. 0 was used to build a prescription database of Chinese medicines treating hypochondriac pain. The software integration data mining method was used to distribute prescriptions according to "four odors", "five flavors" and "meridians" in the database and achieve frequency statistics, syndrome distribution, prescription regularity and new prescription analysis. An analysis were made for 192 prescriptions treating hypochondriac pain to determine the frequencies of medicines in prescriptions, commonly used medicine pairs and combinations and summarize 15 new prescriptions. This study indicated that the prescriptions treating hypochondriac pain in Chinese journal full-text database are mostly those for soothing liver-qi stagnation, promoting qi and activating blood, clearing heat and promoting dampness, and invigorating spleen and removing phlem, with a cold property and bitter taste, and reflect the principles of "distinguish deficiency and excess and relieving pain by smoothening meridians" in treating hypochondriac pain.
Databases, Factual ; Drug Prescriptions ; Drugs, Chinese Herbal ; chemistry ; therapeutic use ; Humans ; Meridians ; Pain ; drug therapy

Acupuncture Therapy ; Aged ; Female ; Humans ; Nervous System Neoplasms ; therapy ; Paraneoplastic Syndromes, Nervous System ; therapy

BACKGROUND: The expression and significance of transforming growth factor-alpha (TGF-α) and epidermal growth factor receptor (EGF-R) in different diseases are different.OBJ ECTTVE: To investigate the expression of TGF-α and EGF-R in human liver cirrhosis tissues.DESIGN : Non-randomized controlled trial.SETTING: Department of General Surgery, Tangdu Hospital, Fourth Military Medical University of Chinese PLA.MATERIALS: This trial was carried out in the Department of Pathology, Xijing Hospital, Fourth Military Medical University of Chinese PLA during March 2003 to May 2004. Sixty-three samples of human liver cirrhosis tissue were obtained from Department of General Surgery, Xijing Hospital, Fourth Military Medical University of Chinese PLA (Informed consents were obtained). Five human normal liver tissues were obtained form autopsy in Department of Pathology, Fourth Military Medical University of Chinese PLA (patients with liver diseases were excluded).METHODS: Sixty-three samples of human liver cirrhosis specimens and five human normal liver tissues were studied with immunohistochemical method and in situ hybridization technique. The positive rates of TGF-α and EGF-R were calculated and managed statistically.MAIN OUTCOME MEASURE: Detection of the expressions of TGF-α and EGF-R in liver cirrhosis tissues were detected with immunohistochemical method and in situ hybridization technique.RESULTS: The expression rate of TGF-α and EGF-R in 63 samples of human liver cirrhosis tissues was 84% (53/63)and 52% (33/63) respectively. These positive granules were brown and most of them were in cytoplasm of hepatocytes.There was significantly positive correlation between TGF-α and EGF-R (r =0.32,P ＜ 0.05). In five normal liver tissues,TGF-α and EGF-R were not detectable in hepatocytes and bile ducts. In situ hybridization showed the levels of TGF-αmRNA and EGF-R mRNA were higher (86%, 54%) than those of immunohistochemistry. There was no significant difference between two detective methods (P＞0.05).CONCLUSrON: Liver cirrhosis might be under the autocrine regulation of TGF-α/EGF-R. Increasing expression of TGF-αand EGF-R might be one of important factors in liver cirrhosis pathogenesis.

Aortic Aneurysm, Abdominal ; therapy ; Humans

Hematopoietic Stem Cell Transplantation ; adverse effects ; Humans ; Male ; Middle Aged ; Purpura, Thrombotic Thrombocytopenic ; etiology ; Transplantation, Autologous

With the development of society and the improvement of people's living standards, the effect of Chinese medicine in treatment and health care is more and more prominent. The herbal decoction pieces are the important part of Chinese medicine,it can be applied directly to clinical treatment and it's also the raw material of Chinese patent medicine. Therefore, the quality of herbal decoction pieces is quite important. The parts of the production of herbal decoction pieces are numerous, and there are possibilities of adverse effects on the quality of the herbal decoction pieces in every part. In this paper, we based on the production chain of herbal decoction pieces, analyzed the main problem that affect the quality of herbal decoction pieces in the part of selection of Chinese herbal medicines, planting, purchasing, processing, packaging, storage and transport, such as the poor quality of seed and seedlings of plant-based Chinese medicines, some plants left their place of origin and have been introduced in the place that is not suitable for this kind of plant, the insufficient growth time and the excessive harmful substances. The purchasers and the accepters lack of professional knowledge and professional ethics. The mechanism of processing is not clear, the standards can not be uniformed, and lack of qualified person in processing, etc. So we suggest: intensify the basic research of key scientific issues. Improve the quality of persons who work in herbal decoction pieces; Establish an "integration" mode of operation in herbal decoction pieces enterprise; Breeding high quality plant resources, establish the large-scale planting basement; Make the packing of herbal decoction pieces standard; Establish the modernization traditional Chinese medicine logistics enterprise.
Chemistry, Pharmaceutical ; economics ; manpower ; standards ; Drug Packaging ; economics ; manpower ; standards ; Drug Storage ; economics ; standards ; Drugs, Chinese Herbal ; chemistry ; standards ; Humans ; Medicine, Chinese Traditional ; economics ; standards ; Quality Control

A review with 58 references is presented on the degradation methods of polysaccharide, preparation of monosaccharide and oligosaccharide derivatives and selection of chromatographic conditions for gas chromatography and high performance liquid chromatographic analysis. The application and advance of chromatography on the analysis of carbohydrates are introduced.

Objective:To study the Cronassial′protective effect on brain tissue during ischemia reperfusion.Methods:The rat cerebral ischemia reperfusion model was prepared by four-vessel occulasion method.The Glu,GABA content in hippocampus during cerebral ischemia reperfusion was observed by high press liquid phase method.Results:Glu,GABA content increased during ischemia and decreased during reperfusion. Cronassial could obviously decrease the Glu content and increase neuron density.Conclusion:Cronassial can inhibit Glu and protect brain tissue during cerebral ischemia reperfusion.