Objective To investigate the changes of serum hs-CRP、ET、MMP-9 and NSE levels and the relationships with neurological function defect in acute cerebral infarction patients,and the influence of edaravone on it.Methods 100 patients with acute cerebral infarction were randomly divided into routine group and edaravone group,the routine group were given routine treatment,the edaravone group were added edaravone 30mg,twice a day,total of 14d;the serum levels of hs-CRP、ET、MMP-9 and NSE and the change of neural function defect were investigated before treatment and 14d after treatment.Results The serum levels of hs-CRP,ET,MMP-9 and NSE were in a positive correlation with the score of neurological function defect(P ＜ 0.05).The edaravone group serum levels of hs-CRP,ET,MMP-9 and NSE were lower than those of routine group in invasion after treatment(P ＜ 0.05).Score of NIHSS in the trial group was lower than that of routine group(P ＜ 0.05).Conclusion The serum levels of hs-CRP,ET,MMP-9 and NSE increase in the patients with acute cerebral infraction.Edaravone can reduce the serum hs-CRP,ET,MMP-9 and NSE levels and promote the recovery of neurological function in patients with acute cerebral infarction.

Objective To investigate the risk factors and prevention methods of anastomotic fistula after laparoscopic sphincter preserving surgery for low rectal cancer.Methods 166 patients with low rectal cancer were treated with laparoscopic total mesorectal excision(TME),34 cases were treated with prophylactic colostomy.The clinical and pathological factors were collected and the χ2 test was used to analyze the influencing factors of anastomotic leakage.Logistic regression analysis was used to analyze the independent risk factors of postoperative anastomotic leakage.Results Anastomotic leakage occurred in 14 cases,incidence rate was 8.43%(14/166).All patients were cured after conservative treatment.Univariate analysis showed that age(>65 years),basic diseases,preoperative albumin(<35 g/L),preoperative intestinal obstruction,tumor size(>4cm),Dukes stage were the risk factors of anastomotic leakage(P<0.05).Multivariate analysis showed that preoperative albumin,preoperative intestinal obstruction,tumor size were the independent risk factors of anastomotic fistula.Conclusion The incidence of anastomotic leakage in low rectal cancer was higher,should be highly valued.Prophylactic stoma should be used for the independent risk factors,which can effectively reduce the probability of the second operation,reduce the degree of fistula,shorten the healing time of fistula.

Objective To improve the transparency of medical service charge and show respect to the patient's right to know and choose.Methods The hospitalization charge data was published from intranet to internet using secure data exchange.Enquiry system for hospitalization charge was designed and implemented.Results Patients and their family members can query their hospitalization charge using internet to know their charge and medication.Conclusion This system can improve the satisfaction of patient,enhance hospital's management,and the social benefit is great.

Objective To determine loganin in productions of Liuweidihuang. Methods The chromatographic column was shim-packvp ODS-C18 (4.6 mm?150 mm,5 ?m),the mobile phase was THF-acetonitrile-methanol-0.05%H4PO3 (1∶8∶4∶87),the detection wavelength was 236 nm,the temperature of column was 40 ℃ and the flow rate was 1.0 mL/min. Results A good linearity of loganin was in the range of 4.42?10-2～22.1?10-2 ?g,r =0.999 7,and the average recovery rate was 98.96% (n =6,RSD=1.42%). Conclusion The method provided a reference for evaluating and controlling quality of series productions of Liuweidihuang.

?Ocular trauma related glaucoma is one of secondary glaucoma, which can lead to serious visual loss. According to the complex clinical findings and pathogenesis of ocular trauma related glaucoma, we divide traumatic secondary glaucoma into hyphema related glaucoma, angle recession related, lens injury related, adhesion and proliferation related. The treatment of secondary traumatic glaucoma with ocular trauma were different, specific treatment measures should be given according to the specific case to protect visual function.

Objectiv To explore the effects of chromium (Ⅵ) on the levels of malondialdehyde (MDA), the final product of lipid peroxidation in peripheral blood of mice and the micronucleus rate of polychromatic erythrocytes (PCE) in peripheral blood. Method 60 Kunming mice were divided randomly into 6 groups averagely. 4 groups were selected as exposure groups which were exposed to 10.27, 13.83, 20.74, 41.48 mg/kg calcium chromate by oral perfusion, one time per day, for 6 days. The negative and positive control group was exposed to distilled water and cyclophos phamide (40 mg/kg) respectively. After exposure, the levels of MDA and micronucleus rates of PCE were determined. Results Significant increases of the levels of MDA and the micronucleus rates of PCE in peripheral blood of mice were observed, which were associated with the increases of the exposure doses of chromium (Ⅵ). Significant positive correlation was observed between the micronucleus rates of PCEs (x) and MDA(nmol/ml) levels (y) in peripheral blood (y=3.705+930x,r=0.958, P

AIM: To observe the efficacy of high concentrations of sodium hyaluronate ( 3g/L SH ) for moderate to severe dry eye.
METHODS: Forty moderate to severe dry eye patients were included in the study according to the diagnosis criteria and randomized into two groups. The patients of the trial group received topical administration of high concentration sodium hyaluronate (3g/L), and those of the control group received sodium hyaluronate ( 1g/L ) plus recombinant human epidermal growth factor. The dry eye symptom scores, ocular surface disease index ( OSDI) scores, tear film break-up time ( BUT) , SchirmerⅠ test and corneal fluorescein staining score were evaluated. All the indexes were compared between the two groups 2wk before and after treatment.
RESULTS: There were no significant differences of the indicators between the two groups before treatment. After 2wk treatment, the differences were statistically significant compared to former except for the SchirmerⅠtest. Compared with the control group, the symptom scores and the OSDI scores were lowered. No significant differences were found in the other indicators between these two groups.
CONCLUSION: Topical usage of highconcentrations of sodium hyaluronate (3g/L) is beneficial for remitting the ocular symptoms in moderate to severe dry eyes, and also improve the quality of life of patients.

PAX6 gene plays an important role in embryological development, and the mutation of this gene may result incongenital aniridia, retinoblastoma, macula hypoplasia, Peters' anomaly and so on. A brief introduction of the background PAX6 gene, and the association between PAX6 and retinal diseases were summarized in this review.

AIM:To evaluate the efficacy of treating traumatic optic neuropathy( TON) with mouse nerve growth factor.
METHODS: We searched the Cochrane Library, Pubmed, Medline, CNKI, Wanfang database and VIP to collect randomized controlled trials ( RCTs ) of using mouse nerve growth factor for TON. The quality of the included trials was assessed and poor-qualified trials were eliminated before the meta - analysis was conducted.
RESULTS:Seven RCTs with a total of 399 eyes included were retrieved, and OR=3. 78 with a 95%CI of [ 2. 35, 6.06], the difference was significant (P<0. 01).
CONCLUSION:The existing evidence supports that the prognosis of TON is better when mouse nerve growth factor are adopted in treatment, but there is still a need for well-planned, large-scale and multicenter RCTs to verify it.

Objective To develop models of epileptic discharge by activating group Ⅰ metabotropic glutamate receptors (mGluR) or by blocking gamma-aminobutyric acid (GABA) receptors on rat hippocampal slices. Methods Rat hippocampal slices were exposed to mGluR group Ⅰ specific agonist dihydroxyphenylglycine (DHPG) or to GABAA receptor antagonists bicuculline methiodide(BMI), and single pyramidal cell in the CA3 region of the slice was recorded by whole cell patch clamp technique. Results Exposure to DHPG or BMI resulted in the induction of spontaneously occurring epileptic discharge in the CA3 region of rat hippocampal slice, and there was no significant difference in the frequency of discharge between them(P>0.05). Conclusion Epileptic discharge can be generated in vitro in response to a loss of balance between excitatory and inhibitory influences.