Hp phenotypes were determined by polyacrylamide disc-electrophoresis in sera from 555 individuals of Han population in Kunming. HP phenotype frequencies were simillar to that of Shanghia, Quandong Han Population and the other six phenotypes (1-p,2-1F, F K etc) could be detected in Kuming population by the same method.

Objective To study the haplotype diversity of DYS390、DYS393、DYS437、DYS438、DYS439、DYS447、DYS448、DYS460、H4 loci in 216 unrelated individuals in Han ethnic group of Kunming city,Yunnan province.Methods The DNA were extracted with Chloroform,phenol methods or Chelex-100 method.STR-PCR,Non-denaturing polyacylaminde gel electrophoresis and Sliver staining and ABI377 sequencer were carried out for study.216 undividuals were collected from Han ethnic group in Kunming city.Results 216 types of haplotype were detected from 9 STRS.Every haplotype frequence was detected at 0.0046,GD=1.00005934437,The lowest arrangement of the repeat units was detected at 0.0046.S E=1.25?10-9。Conclution The 9 Y-STRs loci of Kunming people are highly polymorphic and are powerful means in determination of identify and paternity for forensic practice.

Serious and/or continuous cerebral hemodynamics disorder is the main cause of neonatal brain injury.Monitoring its changes by using simple,accurate and effective techniques has guiding significances in maintaining the stability of neonatal cerebral hemodynamics.This paper aims to introduce several cerebral hemodynamic monitoring techniques,including color Doppler ultrasound,near infrared spectroscopy,noninvasive magnetic resonance imaging,and provide references to the clinical application in neonatal field.

BACKGROUND:Three-dimensional printing technique has been applied in medical fields since it was invented in the end of 20th century. Recently it has been widely used in manufacturing scaffolds for bone tissue engineering. OBJECTIVE:To review the basic concept of the scaffold for bone tissue engineering, the basic requirements for three-dimensional printing technique in scaffold engineering, different materials used in bone tissue engineering, the advantages and limitations of three-dimensional printing technique and the outlook of three-dimensional printing technique applied in manufacturing scaffold for bone tissue engineering. METHODS: The first author did a computer-aided retrieval of the MEDLINE database, Science Direct database, CNKI database, and CQVIP database for articles relevant to three-dimensional printing technique used in manufacturing scaffolds for bone tissue engineering published between January 1990 and February 2015. The key words were three-dimensional printing, tissue engineering, rapid prototyping technology, scaffold, materials in English and Chinese, respectively. Repetitive studies were excluded, and 33 of 52 related literatures were adopted in result analysis. RESULTS AND CONCLUSION: Three-dimensional printing technique has many advantages such as high resolution, high velocity and the freedom to build unlimited geometries. There are some requirements for the powder and binder used to construct bone tissue engineering scaffolds using the three-dimensional printing technique, such as the flowability, stability and wettability. A wide range of materials can be used: synthetic and natural polymers, ceramics, as wel as composites of the aforementioned. Various kinds of powder take responsibility of different features of scaffolds, resulting from the advantages and disadvantages of different materials. Although this technique has some limitations such as high cost and the difficulty of commercial production, its application stil has a bright future.

Objective To investigate the current prevalence of depressive disorder and the associated factors in pa?tients with essential hypertensive from cardiovascular divisions of tertiary general hospitals. Methods A Chinese version of Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders (Fourth Edition) Axis I Disor?ders was conducted by five mental health professionals on 876 essential hypertensive outpatients at cardiovascular divi?sions of tertiary general hospital. Data on the socio-demographic and clinical characteristics of these hypertensive pa?tients were also collected. Results The current prevalence of depressive disorders among essential hypertensive outpa?tients of tertiary hospitals was 16.6%(145/876). Risk factors for depressive disorders in hypertensive patients included fe?male gender (OR=2.817, 95%CI:1.161~6.885), being 65-year-old and older (OR=1.747, 95%CI:1.118~2.688), non-marital status (OR=2.023, 95%CI:1.203~3.398), gradeⅢhypertension (OR=1.776, 95%CI:1.226~2.333) and car?diovascular diseases (OR=2.018, 95%CI:1.427~2.587). Conclusion Essential hypertensive outpatients, who receive med?ical services at cardiovascular divisions of tertiary general hospital, have high prevalence of depressive disorders. Clini?cians should pay more attention to the mental health of outpatients with essential hypertension.

Animals ; Hippocampus ; pathology ; physiopathology ; Male ; Motor Activity ; Rats ; Rats, Wistar

0.05),but the expression of CD19+ in the 3rd month and the 6th month were lower than that of healthy control group(Pa

Objective To explore the ultrasonographic, clinical and histopathological characteristics features of primary uterus lymphoma. Methods The clinical, sonographic and histopathological characteristics of nine patients who were pathologically comfirmed as primary uterus lymphoma were analyzed. Results Among the nine cases of primary uterus lymphoma, three lesions were found in the uterine bodys and 6 were found in the cervixes. Two patients with uterine body lymphoma had painless pelvic masses and six patients with cervix lymphoma had contact bleeding, including irregular vaginal bleeding in four patients. In sonograms, six lesions showed remarkable hypoechogenicity with homogeneous internal echogenicity;the margins of most lesions were circumcribed;all lesions had posterior enhancement. In color Doppler lfow imaging, abundant vascularities were detected in eight lesions. In the cases of uterine body lymphoma, all of the uterine bodys showed diffuse symmetrical enlargement without disruption of the endometria, while in ifve cases of cervix lymphoma, the tumors showed the shape of lobule. The pathological examination showed that all tumors were diffuse B-cell type non-Hodgkin lymphoma. Conclusions Primary uterus lymphoma has some clinical and ultrasonic features, including contact bleeding or irrgular vaginal bleeding, diffuse symmetrical enlargement of uterine body, complete endometria, remarkable hypoechogenicity, homogeneous echogenicity and posterior enhancement. Final diagnosis depends on the histopathology.

Objective To achieve millisecond grade precision in the design model of event-related fMRI experiment.Methods The impact factors of time precision were analyzed,and solutions were put forward.Results The parameter of E-Prime(a software dedicated to psychology study) was adjusted to get millisecond precision.Conclusion General computer is very difficult to achieve millisecond grade due to software and hardware factors,but it can be achieved through adjusting the parameter of E-Prime.

Objective To study the chemical constituents in the root of Baccaurea ramiflora.Methods Various chromatographic techniques were used to separate and purify the constituents.The structures of the compounds were elucidated by physicochemical properties and spectral analyses(UV,IR,ESI-MS,1H-NMR,13C-NMR,Dept90,Dept135,1H-1H COSY,1H-1H NOESY,HMQC,HMBC).Results A new compound was isolated and idertified.Conclusion This compound is a novel compound and named as epidihydrotutin.