Objective To study the negative emotion regulation of sea-going seafarers,establish a regulation strategy questionnaire of sea-going seafarers' negative emotion and examine its reliability and validity.Methods Based on open-ended questionnaires and previous researches,the initial questionnaire was obtained.Randomly selected 188 sea-going seafarers,by adopting program analysis and factor analysis,the final questionnaire was determined.Tested 121 sea-going seafarers by using the final questionnaire,while 55 of them by using coping style questionnaire at the same time.After two weeks,47 of them were tested by final questionnaire again.The reliability and validity of the questionnaire were investigated during the research,and the criterion validity between the coping style questionnaire and regulation strategy questionnaire of sea-going seafarers' negative emotion was also studied.Results The factor analysis results showed that the questionnaire was composed of seeking for support,abreaction,inobservance,transfer and oppression.Cronbach α of each factors was 0.857,0.841,0.825,0.806,0.754 respectively;and Cronbach o of the entire questionnaire was 0.873.The split-half correlation coefficients were 0.697,-0.839 (P ＜ 0.01) ; test-retest reliability coefficients were 0.706,-0.822 (P ＜ 0.01).The correlation between subscales were-0.031,-0.459 ; and the correlation between subscales and total scale were 0.590,-0.806 (P ＜ 0.01).Criterion validity showed that despite for the abreaction,each factor was significantly correlated with the factors of coping style questionnaire.Conclusion The sea-going seafarers' negative emotion regulation strategy questionnaire has acceptable psychometrics properties on reliability and validity.

BACKGROUND: There are obvious neuropsychological changes after stroke besides the damage of cognitive function, and the symptoms of depression and anxiety which are also very conspicuous.OBJECTIVE: To investigate the related characteristics of neuropsychological changes after stroke by means of neuropsychological methods.DESIGN: A case-control study.SETTING: Department of Psychology, the Third Hospital of Daqing; Department of Neurology, the Second Affiliated Hospital of Harbin Medical University.PARTICIPANTS: Sixty stroke inpatients (33 males and 27 female)aged from 42 to 75 years old with an average of (59.3±8.8) years old were selected from the Department of Neurology, the Second Affiliated Hospital of Harbin Medical University between October 2001 and June 2002. Totally 49 cases had cerebral infarction and 11 cases had cerebral hemorrhage; 31 cases had single focus and 29 cases had multiple focuses (including 2 focuses or more); 20 cases had lesion in left hemisphere,21 cases had lesion in right hemisphere and 19 cases had bilateral lesions. The lesion was at temporal lobe in 6 cases, at occipital lobe in 2 cases, at frontoparietal temporal lobe in 15 cases, at internal capsule in 4 cases, at basal nuclei in 19 cases, at lateral ventricle in 4 cases, at thalam us in 4 cases, at cerebellum in 1 case and at multiple sites in 5 cases. The focal size was ＜ 15 mm in 33 cases and ≥ 15 mm in 27 cases. Sixty residents (33 males and 27 females) aged 42to 75 years with an average of (58.7±7.9) years old were selected simultaneously from the communities and suburbs of Harbin city as the controls and they all had no cerebral and organic lesion, mental disorder, severe somatic diseases and family history of mental disease.INTERVENTIONS: The poststroke mental status of the patients at stable recovery period was assessed with symptom checklist-90 (SCL-90, consisted of 90 items and 9 symptoms, scored by 5 grades of 0 to 4, including 0 as never, 1 as mild, 2 as moderate, 3 as a little severe and 4 as severe;Hamilton depression scale (HAMD, consisted of 24 items, including 11 somatic symptoms scored by 0 to 2 grades and 13 mental symptoms scored by 0 to 4 grades; the total score ＞ 24 points was taken as severe depression, 17 to 24 as moderate depression, 7 to 16 as mild depression, ＜ 7 as no depression), self-rating depressive scale (SDS, consists of 20 items, evaluated according to the occurrence frequency of the symptom, and classified into 4 grades; the score was 1, 2, 3 and 4 points for the forward scored questions, but 4, 3, 2 and 1 point for the backward scored questions; the highest total score was 80 points; the depressive severity=accumulative scores of each item/80×100, the depressive index ranged 25 to 100, the index ＜ 0.5 was taken as no depression, 0.5 to 0.59 as mild depression,0.6 to 0.69 as moderate depression, ＞ 0.7 as severe depression) and selfrating anxiety scale (SAS, consisted of 20 items, scored by 4 grades, mainly evaluated according to the occurrencefrequency of the symptoms. The forward scores were 1, 2, 3 and 4 points, and the backward scores were 4,3, 2 and 1 point; the total score multiplied by 1.25, and then the integer was taken as the standard score, the lower the better, and the delimitative value was 50. The total score of anxiety ＜ 50 points was taken as normal,50 to 60 as mild anxiety, 61 to 70 as moderate anxiety, ＞ 70 as severe anxiety). According to the different characters of disease, the patients with moderate depression and above were given suitable psychotherapy and psychiatric drugs for 4 weeks as a course.of the 4 scales between stroke patients with different lesion natures, foin the patients accompanied by depression. RESULTS: All the 60 stroke patients and 60 eases in the control group were involved in the analysis and SAS between the stroke group and control group: All the total scores were higher in the stroke group than in the control group [(131.45 ±18.89), (94.25 ±9.08) points; (18.73 ±6.54), (8.60 ±2.39)points; (56.49±11.85), (41.63±9.91) points: (40.08±6.55), (28.72±1.84)the 4 scales between stroke patients with different lesion natures, focal sites and focal sizes: In the stroke group, the total scores were higher in the patients with lesion in left hemisphere, cortical lesion, multiple focuses and large focal size (≥ 15 mm) than in those with lesion in right hemisphere, subcortical lesion, single focus and small focal size (＜ 15 mm)(t=1.92 to 4.31, P ＜ 0.05), but the scores were almost the same between the patients with cerebral infarction and those with cerebral hemorrhage accompanied by depression before and after treatment: 21 patients had moderate depressive symptoms and above, and their total scores of SCL90, HAMD, SDS and SAS were obviously higher than those in the control group (t=7.45 to 13.75, 9.83 to 21.20, P ＜ 0.01).CONCLUSION: The poststroke neuropsychological changes are more obvious, and the changes are associated with the lesion location, focal size and focal site, but have no close correlation with the lesion nature.After psychological interventions, the depressive emotion is markedly ameliorated in the patients with obvious depressive symptoms and it is then indicated that poststroke patients should be given psychological interventions.

We present the binding ability of a new peptide (CMPQVMPMC-) with dental enamel after being evaluated in the present study. Under a standard procedure, the recovery of M13 filamentous phage was greatly enhanced by displaying the peptide in phage coat protein p III. Then the cyclic peptide was synthesized using a solid method. The effect of the cyclic peptide in vitro biomineralization was tested in a single-diffusion microtiter plate gel system. Absorbance at 405 nm of each sample was recorded for 24 h at every 6 h intervals. The relatively increased values of each sample were expressed as percentages relative to the blank group (100%). The cyclic peptide resulted in a concentration-dependent delayed nucleation. In addition, the overall values of peptide groups at the end of 24 h were lower than those in the control group but much higher than those in the BSA control group.
Dental Enamel ; chemistry ; Peptides ; chemical synthesis ; chemistry ; Protein Binding ; Tooth Calcification

Objective: To study and explore the distribution characteristics of rs10975521A/T and rs1929992A/G polymorphism of the IL-33 gene in Chinese Guangxi population. To compare the frequency distribution differences of allele and genotype of two sites among different ethnic. Methods:The polymorphism of rs10975521A/T and rs1929992A/G of IL-33 gene in 283 subjects were analyzed with the methods of Single base extension (PCR-SEB) and DNA sequencing,and the distribution frequency and the differences between groups of that were analyzed statisticaly. Results:Three genotypes of AA,AT and TT were found in rs10975521A/T with the frequency distribution of 12. 7%,53. 0% and 34. 3% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population ( P>0. 05 ) . There were significant differences of the allele frequency of rs10975521A/T in the Guangxi population compared with that in the European (P< 0. 05), han Chinese in Bejing (P< 0. 05) and Japanese people (P< 0. 01). Three genotypes of AA,AG and GG were found in rs1929992A/G with the frequency distribution of 23. 9%,53. 7% and 13. 4% respectively and there was no significant difference between sexes of each genotype and allele frequency in the Guangxi population(P> 0. 05),but the differences of genotype frequency of rs1929992A/G was statistically significant compared with that in the European,han Chinese in Bejing and Japanese people ( P< 0. 05 ) . There were significant differences of the allele frequency between Guangxi population and European (P< 0. 01),han Chinese in Bejing(P< 0. 05). Conclusion: There are different degrees of discrepancy of rs10975521A/T and rs1929992A/G polymorphism of IL-33 gene among different race and region.

Objective To conduct bioinformatics analysis of children with severe malaria to find out the key gene changes in order to provide a new basis for the prevention and treatment of malignant malaria.Methods Microarray gene chip data was downloaded from public databases GEO and imported into the analysis software STRING,PANTHER and GenClip.The gene expression profiles,protein interaction networks,the process of molecular biology,gene function were analyzed.Results 623(1.93 ％) differentially expressed genes had a good diagnostic capabilities in the diagnosis of mild and severe malaria.OAS2,OAS3,IFIT3 and USP18 were the core sub-network node of the Protein-Protein Interactions.Differentially expressed genes mainly involved in the body's immune defense,immune response,response to external stimuli,the biological function of type 1 interferon activation pathways.Conclusion The progress of malaria of children may be in the regulation of OAS2,OAS3,IFIT3,USP18 and children's immune defense capacity decreased,the malaria began to progress more easily.

0.05). Conclusions The expression of angiotensinⅡincreases in BPH tissues in rats. It is suggested that angiotensinⅡmay affect cell proliferation but not significantly affect cell apoptosis of the rat prostate.

OBJECTIVETo explore the clinical effects of minor bone anchors and palmaris longus tendon graft in treating chronic mallet fingers deformity.

METHODSFrom January 2008 to June 2013, 26 patients with chronic mallet fingers deformity were treated with minor bone anchors and palmaris longus tendon graft. There were 18 males and 8 females, aged from 18 to 52 years old with an average of (32.0±1.3) years. Among them, 8 cases caused by machine injury, 6 cases by fall injury, 6 cases by sprain from fight, 4 cases by tendon spontaneous rupture, 2 cases by knife trauma. There was no tendon attachment of extensor tendon check in 16 cases, and with 0.3 to 0.5 cm tendon attachment in 10 cases. All patients had the flexion deformity and the disability of dorsiflexion activity. During operation, the distal interphalangeal joint was fixed in 10° to 20° dorsiflexion by a Kirshner wire, the minor bone anchor was used to reconstruct the extensor tendon insertion, the palmaris longus tendon slice was transplanted the decayed area of extensor tendon insertion. Four weeks postoperatively, the Kirshner wire was removed and the plaster external fixation was used, and the patient began function exercises. Postoperative complications were observed and fingers functions were assessed according to Dargan standard.

RESULTSThe patients were followed up from 6 to 14 months with an average of (5.0±0.3) months. Wound superficial infection occurred in 2 cases, the skin pressure ulcer in 2 cases, joint activities disability in 1 case; these symptoms got improvement after symptomatic treatment. Traumatic arthritis occurred in 2 cases, 1 case was improved after treatment, and 1 case had chronic pain for a long time. No internal fixation loosening or breakage and tendon rupture were found. According to Dargan standard to evaluate the finger function, 17 cases got excellent results, 8 good, and 1 poor.

CONCLUSIONIt is an effective way to treat the chronic mallet finger deformity using minor bone anchors and palmaris longus tendon graft, and the method has advantages of reliable fixation, easy operation, satisfactory effect and less complication.

Adolescent ; Adult ; Female ; Finger Injuries ; surgery ; Fracture Fixation, Internal ; Hand Deformities, Acquired ; surgery ; Humans ; Male ; Middle Aged ; Suture Anchors ; Tendon Transfer

Objective To investigate effect of paclitaxel on expression of programmed death ligand-1 ( PD-L1 ) in the surface of cervical cancer TC-1 cells and its mechanism. Methods ①The cells were divided into two groups: paclitaxel group, paclitaxel combined with PKD blocker (G? 6976) group. There were 4 concentration gradient and 5 holes for each group, and each hole has its corresponding concentration of drugs. Influence of paclitaxel on TC-1 cell viability and effect of PKD blocker G? 6976 on IC50 value of paclitaxel were evaluated by MTT method.②The cells were divided into 0. 9% sodium chloride solution ( NS) group and paclitaxel group, There were 5 holes of each group. Effect of paclitaxel on PD-L1 expression on the surface of TC-1 cells were measured by immunohistochemistry.③The cells were divided into 4 groups:NS+DMSO group, G? 6976 group, paclitaxel group and paclitaxel+G? 6976 group. There were 5 holes for each group. Effect of paclitaxel and G? 6976 on PD-L1 expression on the surface of TC-1 cells were measured by immunohistochemistry. The expressions of PD-L1 on the surface of cells were measured by immunofluorescence treated with different drugs. Results The IC50 value of paclitaxel was 40 μg·mL-1 in paclitaxel group, and 38. 9 μg·mL-1 in paclitaxel combined with PKD blocker G? 6976 group, without significant difference between the two groups (P>0. 05). The expression of PD-L1 in the surface of TC-1 cells were significantly higher in paclitaxel group than in negative control group [(88. 48±13. 44)% vs. (39. 59±5. 99)%, P<0. 05]. The expression of PD-L1 in the surface of TC-1 cells was (79. 7%±4. 7)% after treatment with paclitaxel combined with PKD blocker G? 6976 for 24 h, and it was significantly lower than that in paclitaxel group [(96. 8±2. 5)%, P<0. 05]. Conclusion Paclitaxel promotes the expression of PD-L1 in the surface of TC-1 cells, which could be significantly inhibited by blocking PKD pathway. Paclitaxel may exert its effect through PKD pathway.

From June 1997 to June 2001 intraoperative transesophageal echocardiography(TEE)was used in 493 patients undergoing different cardiovascular operations including correction of congenital heart defect, coronary artery bypass grafting(CABG),cardiac valve replacement and major vascular surgery.The clinical data of these cases were complete and detailed enough for retrospective review.Their cardiac functions were graded to be classⅡ-Ⅳaccording to New York Heart Association(NYHA)classification.The patients ranged in age between 1.6-74 yrs and weighed between 8.5-92 kg.The probe of TEE was inserted in esophagus after tracheal intubation.Preoperative diagnosis was found to be wrong by TEE in 20 cases(4.1%).Additional defect was found in 51 cases(10.3%).The type of operation planned before operation was altered in 52 cases(10.5%). During the operation real time TEE revealed that the operation performed failed to achieve the goal and revision surgery was needed in 24 cases(4.9%).Abnormal hemodynamics and cardiac function were found by TEE after the heart resumed spontaneous beat in 17 cases(3.5%)and appropriate treatment was instituted.Oral and pharyngeal mucous membrane bleeding occurred in 7 cases.There were no serious complications attributable to TEE.Intraoperative TEE is a very useful tool in formulating the surgery,monitoring hemodynamics and assessing immediate results of surgery and is safe and reliable in the hands of trained anesthesiologists.

ObjectiveTo comprehend the progress of perforator flap research and speculate its developmental trends.MethodsPubMed search was conducted to retrieve the papers published before November,2011.Analyze the literatures about perforator flap on years,nationalities,languages,journals and frequencies of the key words by using Endnote 8.0. ResultsAll 1719 literatures about perforator flap have been collected by PubMed till Oct.31,2011.Lots of them,amount to 51.5 percent,were from USA,China,Japan and UK. ConclusionEnglish was the main publishing language.The articles,which were mainly published by the Plast Reconstr Surg and other 10 journals,add up to 78.7％.The research emphases of the perforator flap will be breast reconstruction, repair defect, deep inferior epigastric artery perforator flap, anterolateral thigh flap,thoracodorsal artery flap,perforator flap of gluteal artery,and posterior leg perforator flap.Angiography and 3D-reconstruction,immunohistochemistry,and neovascularization are the new trend in flap research.