1.Facile Solvothermal Synthesis of Reduced Graphene Oxide-BiPO4 Nanocomposite with Enhanced Photocatalytic Activity
Peng HU ; Jing NIU ; Miao YU ; Shuangyan LIN
Chinese Journal of Analytical Chemistry 2017;45(3):357-362
Reduced graphene oxide-BiPO4 ( RGO-BiPO4 ) nanocomposite was synthesized successfully via a one-pot solvothermal method using graphene oxide and bismuth nitrate as precursors and glycerin as solvent at 200℃ for 1 h. The morphology and structure of as-prepared nanocomposite were characterized by SEM, TEM, XRD, XPS, SERS and UV-Visible spectrum. The photocatalytic activity of the nanocomposite was evaluated by the photodegradation of Rhodamine B ( RhB) dye under UV irradiation and it was found that RGO-BiPO4 nanocomposite possessed higher photocatalytic activity than that of pure BiPO4 . RhB could be decomposed 87. 5% within 2 h. Under the same conditions, only 45. 7% of the RhB dye could be decomposed by BiPO4 . The enhancement of photocatalytic activity could be attributed to the effective charge separation due to the electron-accepting and transporting properties of graphene.
2.Cellular Apoptosis of C6 Mouse Glioma Cells Induced by hING4 Mediated by Adenovirus
Yao-Dong ZHAO ; Jing-Cheng MIAO ; Hai-Feng ZHANG ; Wei-Hu SHENG ; Li MIAO ; Yu-Feng XIE ; Ji-Cheng YANG ;
China Biotechnology 2006;0(07):-
The known members of inhibitor of growth (ING) gene family are considered as candidate tumor suppressor genes. ING4, a novel member of ING family, is recently reported to regulate brain tumour angiogenesis through transcriptional repression of NF-?B-responsive genes, induce G2/M arrest by the increased p21 expression in a p53-dependent manner, suppress the loss of contact inhibition and represses activation of the hypoxia inducible factor, which plays an important role in the progression of tumorigenesis. However, seldom studies about ING4 inducing tumor cells apoptosis were reported.The C6 cells (mouse glioma cells) were infected respectively with the blank adenovirus carrying GFP (Ad) and the recombinated Ad-hING4-His, then RT-PCR assay was used to detect the transcriptions of hING4, as well Western-blotting assay was ued to detect the expressions of hING4. The effects of hING4 expression upon C6 cells were observed, and the growth curve was drawed and tumor control rates were calculated. The C6 cells, which were affected by blank Ad and Ad-hING4-His, were respectively observed by LSCM (laser scan confocal microscope) and transmission electron microscope (TEM), detected by flow cytometry; and the genomic DNA of both groups were extracted and electrophoresised in agarose gel to examinate the DNA fragments. The results showed hING4 can significantly inhibit the growth of C6 cells by promoting the cell’s apoptosis, which probably is the first one to prove this property of ING4.The experimental and theoretical foundation for gene therapy for gliomas with ING4 in the future was established.
3.Expression of MPO and TGF-β1 in Ulcerative colitis
Yanying WANG ; Lifang SUN ; Jing YU ; Yanfeng WANG ; Zhiwei YANG ; Xiaoguang MIAO ; Peiyi ZHANG ; Yindong WANG
Journal of Chinese Physician 2009;11(1):31-33
Objective To explore the expression and effect of MP0 and TGF-β1 in ulcerative colitis tissues.Methods The expressions of MPO and TGF-Bl in colonal tissue specimens were determined by immunohistochemistry(two step method)in 37 cases of ulcerative colitis,10 crohng disease and 20 normal mucosa.Results The positive rates of MPO and TGF.B1 were 10%and 20.0%in the normal mucosa,55.0%and 35.O%in crohn's disease,and 89.2%and 86.5%in ulcerative colitis.Expression of MPO and TGF-β1 in the ulcerative colitis were higIIer than that in crohn's disease and normal mucosa(P<0.05),there was correlation between MPO and TGF-β1(r=O.51,P<0.05).Conclusion MPO and FGF-β1 are involved in the pathology of UC,and detection of MPO and TGFβ1 can provide some value to evaluate the conditions of UC.
4.Safety evaluation of niuhuang jiedu tablet.
Yu-Ling FENG ; Jia-wei MIAO ; Jing LI ; An-Sheng SUNG ; Jie LIU
China Journal of Chinese Materia Medica 2014;39(17):3221-3225
Realgar-containing Niuhuang Jiedu tablet (NHJD) has been applied in clinic for more than 800 years. However, because realgar contains arsenic (As), it has aroused wide concerns and controversies both at home and abroad. Currently, there are two misunderstandings about realgar-containing Chinese patent medicines. First, some people exaggerated realgar's toxicity as that of arsenic. Second, they recommended to remove realgar from traditional Chinese medicine compounds. In this paper, the authors summarized the advance in studies on NHJD, and proposed different opinions: (1) It is inappropriate to take total As as the index in safety evaluation of NHJD. (2) The toxicity of NHJD is dependent on the dose and duration of administration. (3) Realgar is an active ingredient of NHJD, and shall be deeply studied. Classic realgar-containing traditional Chinese medicine prescriptions, such as Niuhuang Jiedu tablet, shall be evaluated with rigorous modern scientific basis, with the aim to guide rational and safe application.
Animals
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Arsenicals
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adverse effects
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chemistry
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therapeutic use
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Chemistry, Pharmaceutical
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methods
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Dose-Response Relationship, Drug
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Drugs, Chinese Herbal
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adverse effects
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chemistry
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therapeutic use
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Humans
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Medicine, Chinese Traditional
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adverse effects
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methods
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Sulfides
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adverse effects
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chemistry
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therapeutic use
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Tablets
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Time Factors
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Treatment Outcome
5.Clinical study on pubococcygeal line determined by dynamic magnetic resonance imaging used in evaluating pelvic organ prolapse
Yali MIAO ; Xiaohong ZHANG ; Jing WU ; Yu KANG ; Nan HONG ; Jianliu WANG
Chinese Journal of Obstetrics and Gynecology 2010;45(12):900-903
Objective To investigate clinical value of pubococcygeal line (PCL) determined by dynamic magnetic resonance imaging (MRI) used in evaluating pelvic organ prolapse (POP), and investigate the relationship of pelvic organ prolapse quantitation (POP-Q) stage and pubococcygeal line (PCL) for the patient with POP. Methods Twenty patients with POP were evaluated by POP-Q stage and pelvic dynamic MRI examination simultaneously. Sagittal MRI images were acquired at rest and during maximal Valsalva using a fast gradient echo sequence two-dimensional fast low angle shot (FLASH) T1weighted image. The degree of prolapsed anterior vaginal wall, uterus and posterior vaginal wall were measured by PCL and compared with POP-Q system. Results There were 20 cases with cystocel diagnosed by POP-Q staging system, in which bladder neck or bladder base of 17 patients were under the PCL during maximum Valsalva. The concordance rate was 85% (17/20) between PCL and POP-Q stage. There were 19cases with rectocele diagnosed by POP-Q, in which the anorectal junction of 4 patients' PCL descent below more than 2. 5 cm. The concordance rate was 4/19 between PCL and POP-Q stage. There were 14 cases with uterine prolapse diagnosed by POP-Q staging system, in which uterine cervix of all descent below PCL. The concordance rate was 14/14 between PCL and POP-Q stage. However, it was noted that 5 cases did not reach POP-Q staging and their lowest uteri cervix were below or above PCL but less than 1 cm at maximal Valsalva. Conclusions Compared with POP-Q staging system, the reference line of PCL determined by dynamic MRI could diagnose uterine prolapse accurately and anterior vaginal wall with greater clinical value,however it was limited in diagnosing posterior vaginal wall prolapse effectively. Therefore, the clinical value of PCL should be further studied for evaluating POP.
6.The study of evaluation index system of Knowledge, Attitude, and Practice Model of esophageal speech training for Otolaryngology nurse
Chunyan GUAN ; Qing CHEN ; Miao WANG ; Jingjing YU ; Jing QIU ; Danni JIAN ; Ye ZHANG
Chinese Journal of Practical Nursing 2021;37(10):762-767
Objective:To develop evaluation index system of Knowledge, Attitude, and Practice Model of esophageal speech training for Otolaryngology nurse, which can provide an initial tool to evaluate the effect of esophageal speech training for Otolaryngology nurse.Methods:Based on the Knowledge, Attitude, and Practice Model and literature research, the initial dimensions and items were developed. The evaluation index system was completed by Delphi technique that 23 experts were invited to evaluate the importance of the dimensions and items in the consulting round. Then the weight value of the index system was computed through analytic hierarchy.Results:For the first and second rounds of the study, respectively, the recovery rates were 78.26% and 100.00%, the expert authority were 0.85 and 0.86, and the Kendall′s W were 0.16 and 0.19 ( P<0.05). Finally, the index system contained 3 dimensions and 30 items. Conclusions:The evaluation index system has the possible scientificity and reliability, and has the feasible thoery and content. In the future, the evaluation index system can be used to evaluate the effect of esophageal speech training for Otolaryngology nurse through the clinical practice improvement.
7.Effect of fluid loading on the prognosis of severe hand foot mouth disease in children
Zhuo LI ; Jun CHEN ; Jun SHI ; Jing HU ; Hongjun MIAO ; Tao JIANG ; Wenliang YU ; Jun LI
The Journal of Practical Medicine 2016;32(17):2844-2846
Objective To evaluate the effect of fluid load on the prognosis of severe hand , foot and mouth disease in children. Methods The patients with severe hand foot and mouth disease in the emergency department of PICU in our hospital were enrolled as the research object. We would collect demographic characteristics , labora-tory tests and clinical data: age, gender, focus, basic disease, and simplified acute severity score (SAPS)Ⅱ and record the cumulative amount of fluid balance at 24, 48, 72 hours after admission. Results There was a signifi-cant difference on fluid balance at 48 and 72 hours between the survival group and the death group , the death group appeared the positive liquid balance , and there were significant differences in PICU retention time , mechani-cal ventilation rate, MODF involved organs, mortality and other prognostic indicators between the negative fluid balance group and the positive fluid balance group. Conclusion Fluid balance is an important treatment for severe hand foot and mouth disease, and positive liquid balance is related to mortality and other adverse prognosis.
8.Hepatic arterial chemoembolization via a coaxial microcatheter combined with radiofrequency ablation for the treatment of primary hepatocellular carcinoma
Jiakai LI ; Jing ZHANG ; Miao YU ; Jinshan ZHANG ; Li YANG ; Yueyong XIAO
Journal of Interventional Radiology 2001;0(05):-
Objective To discuss the therapeutic efficacy of transcatheter arterial chemoembolization(TACE) via a coaxial microcatheter combined with radiofrequency ablation(RFA) for primary hepatocellular carcinoma(HCC).Methods The preoperative DSA and multi-slice CT findings in 1 000 patients with HCC,encountered from May1998 to May 2007,were retrospectively analyzed.In 179 cases,the lesion was limited in one hepatic segment,and super-selective catheterization TACE with a coaxial microcatheter was performed in these patients.Four weeks after TACE,dynamic enhanced CT and / or MR scanning was made to observe the results.In 40 cases,there was poor lipiodol deposit in the lesion,and CT-guided RFA was employed for these patients.Follow-up check was done one month after the treatment.Results DSA examination totally revealed 670 lesions with diameter larger than 3 cm,202 lesions with diameter smaller than 3 cm,400 satellite nodules,482 arteriovenous fistulae or arterio-portal shunts,430 abnormal blood-supplying vessels and 362 cancerous thrombosis in portal vein.Four weeks after microcatheter embolization,the local control rate of the tumor was 77.6%.RFA was carried out for patients with poorly-controlled tumors,and one month after RFA the local control rate of the tumor reached 97.5%.Conclusions DSA is the most powerful examination means in detecting lesions less than 3 cm,satellite nodules,tumor’s blood-supply,arteriov-enous fistulae and arterio-portal shunts.Therefore,DSA plays an important role in making the preoperative evaluation of HCC,undoubtedly,this role can not be substituted by any other equipment.RFA is an effective treatment for HCC as well as an ideal alternative for patients who show poor response to TACE.
9.The Study on Fermentation Conditions of Inulase Produced by Aspergillus niger
Ze-Hong CAO ; Yu-Wei DONG ; Jing-Zhi MIAO ; Zhao-Qi LV ;
China Biotechnology 2006;0(08):-
The high production inulase strain was screened from the soil sample where burdock planted in Qin Village,Bayou Town,Pei County,Xuzhou.Inulase activity were determined which produced by 40 strains separated from soil.Three mold stains,C122803、D081506 and D081513,which had higher ability of producing inulase were obtained by using transparent circle method as initial screening and rocker method as re-screening.Enzyme activity of the three strains were 1.411U/ml,1.895U/ml,1.792U/ml,separately.Enzyme activity of D081506,1.895U/ml,was the highest.The fermentation conditions of D081506 were studied and the optimized conditions were lappa juice 2.0%,yeast extraction 1.6%,(NH4)2SO4 0.5%,NaCl 0.5%,K2HPO4 0.5% and pH 5.0.Inulase activity of D081506 was 2.9578U/ml which increased 56.09% under the condition of 27℃,140r/min,24h.
10.Development and validation of a rapid and robust LC-MS/MS method for quantifi-cation of a new oxazolidinone antibiotic MRX-I in human plasma and urine
Miao ZHAO ; Xiaojie WU ; Jun HUANG ; Jicheng YU ; Jing ZHANG ; Beining GUO
Chinese Journal of Infection and Chemotherapy 2014;(3):210-215
Objective To establish and validate an ultra performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS)method for quantification of MRX-I,a new oxazolidinone antibacterial agent,in human plasma and urine.Methods Chromatographic separation was performed on a Waters ACQUITY UPLC BEH C8 column using an isocratic elution.The mo-bile phase consisted of acetonitrile and water (40∶60,v/v).Quantitative analysis was conducted in the multiple reaction moni-toring mode.Linezolid was used as an internal standard.Liquid-liquid extraction with ethyl acetate was used to remove impuri-ties in the plasma and urine samples.The method was validated in terms of matrix effect,recovery,precision,accuracy and stability.Results The calibration curves were linear within the range of 0.005 00-1 .00 mg/L.The lower limit of quantification was 0.005 00 mg/L for both plasma and urine samples.Retention time was less than 1 .5 min for both MRX-I and internal standard in plasma and urine.The ma-trix effect factors of plasma and urine for MRX-I was 90.4%±8.2% and 82.7%±7.9%,respectively.The recovery of MRX-I was 112.8% ± 13.4% from plasma and 105.6% ± 13.4% from urine samples,respectively.The inter- and intra-day accuracy of MRX-I was 98.9%-105.0% and 96.5%-102.6% in plasma samples,and 92.7%-98.6% and 95.1 %-105.7% in urine samples.MRX-I was stable for 24 h at room tem-perature,48 h in automatic sampler after pretreatment,and stable after 3 freeze-thaw cycles in plasma and urine.MRX-I was also stable at-40℃for eight months in plasma and six months in urine,respectively.Conclusions The UPLC-MS/MS method established in this study shows high sensitivity and specificity for determination of MRX-I in human plasma and urine.The re-sults of validation are consistent with the requirement of bioanalytical method validation.