Objective:To study the expressions of hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in fresh and two preserved amniotic membranes (AM),and to explore their biological vitality.Methods:The amniotic membranes from the healthy puerperia with abdominal delivery were randomly divided into three groups:fresh group,DMEM/glycerol preserved group at -80℃ and glycerol preserved group at 4℃ (for 1 and 3 months).Immunohistochemical method was used to investigate the changes in the expressions of HGF and bFGF among the three groups.Results:HGF and bFGF were distributed in the epithelium of AMs mostly.The expressions of HGF in both preserved AMs for 1 and 3 months decreased significantly compared with fresh AM (P

Humans ; Multiple Myeloma ; Neoplasm Recurrence, Local

Objective　To study on effects of mVEGF and anti-VEGF antibody during cultured HUVEC proliferation in vitro.Methods　Endothelial cell proliferation was assayed using human umbilical vein endothelial cells stimulated with mVEGF and with CIA joint extracts and was used　　3　　H-TaR incorporation.Results　The anti-VEGF neutralizing antibody can inhibit the proliferation of HUVEC stimulated with mVEGF and with CIA joint extracts,whose suppression percents were 72.2% and 69.9%,respectively.Conclusion　mVEGF specifically promotes the growth of vascular endothelial cells.During early stage of CIA development,expression of VEGF in the joint increases and VEGF is expressed biologically active and can be inhibited by anti-VEGF neutralizing antibody.

?AlM: To observe the changes of corneal topography and astigmatism et al with various duration of closing eyes and sleep;and study the impact of closing eye and sleep causing physiological hypoxia on corneal topography.
? METHODS: Sixteen volunteers were selected ( 32 eyes), 22-33 (26. 19 ± 3. 95) years old, without refractive errors and other eye diseases, as well as never wearing corneal contact lens, including 10 male eyes and 22 female eyes. The main parameters received corneal simulation K value, corneal astigmatism, corneal irregular measure ( ClM ) and the shape factor ( SF ) by Zeiss corneal topographer. They were measured before and after closed eyes 10, 20, 30, 60, 120min; before sleep and after over 6h sleep, they were detected immediately as soon as they opened eyes after 10, 20, 60, 120min.
? RESULTS: During closing eyes, there were no statistical significance on the changes of corneal topography, corneal astigmatism, and SF. After opening eyes and over 6h sleep, there were no statistical significance on the changes of corneal topography, corneal astigmatism, ClM, and SF.
?CONCLUSlON:Closing eyes and sleep don’t injure the cornea substantially, so corneal topography and corneal astigmatism don’t mainly change.

AIM: To investigate the effect and mechanism of Mingjing decoction combining with argon laser on the levels of plasma vascular endothelial growth factor (VEGF) in patients with diabetic retinopathy(DR).
METHODS:The 66 patients(118 eyes)with DR at Ⅲ ~ Ⅴstage were randomly divided into two groups (treatment group and control group). The control group (33 patients with 56 eyes ) was treated with argon laser, and the treatment group (33 patients with 62 eyes) was treated with mingjing decoction combining with argon laser, and a blank group(30 eyes) was set up. The levels of plasma VEGF were detected by enzyme linked immunosorbent assay (ELISA). The results of best corrected visual acuity and fundus fluorescein angiography(FFA) were analyzed, as well as detected glycosylated hemoglobin ( HbA1c), blood coagulation function and the function of liver and kidney.
RESULTS: Patients with DR had significantly risen plasma VEGF before treatment when compared with blank control group ( P < 0. 05 ). After 3mo of combined treatment, the levels of plasma VEGF significantly reduced and HbA1c decreased in patients treated with mingjing decoction and argon laser, there were statistically significant difference compared to control group ( P < 0. 05 ). In the treatment group, the best corrected visual acuity and FFA were significantly improved in patients treated with mingjing decoction combining with argon laser compared to patients treated with argon laser alone after 3mo of combined treatment. CONCLUSION: Mingjing decoction combining with argon laser for DR can effectively reduce the level of plasma VEGF, stabilize blood sugar levels, improve the function of retina, and delay DR progresses.

Objective To observe the clinical efficacy of acupuncture-moxibustion in treating endometriosis (EM). Method Fifty EM patients were randomized into two groups, 25 cases in each group. The treatment group was intervened by acupuncture-moxibustion, while the control group was intervened by oral administration of Mifepristone tablets. Abdominal pain, pelvic lump, and relevant indicators were observed before and after the treatment, and the therapeutic efficacies were compared.Result After 6-month treatment, symptoms including abdominal pain obviously subsided in the treatment group and the total effective rate was 92.0%, versus 52.0% in the control group, and the between-group difference was statistically significant (P<0.01). The pelvic lump size was significantly changed after the treatment in both groups (P<0.05), suggesting that the lump size became smaller in both groups after the treatment;there was a significant difference in comparing the pelvic lump size between the two groups after the treatment (P<0.05), indicating that the lump size was reduced more significantly in the acupuncture-moxibustion group. The level of serum CA125 changed significantly after the treatment in both groups (P<0.05), suggesting that serum CA125 dropped in both groups after the treatment; there was a significant difference in comparing the level of serum CA125 between the two groups after the treatment (P<0.05), indicating that serum CA125 dropped more significantly in the acupuncture-moxibustion group. A year later, there was a significant difference in comparing the relapse rate between the two groups (P<0.05), revealing that the relapse rate was significantly lower in the acupuncture-moxibustion group than in the medication group.Conclusion Acupuncture-moxibustion can produce a marked therapeutic efficacy in treating EM.

Objective To investigate the risk factors of ischemic cerebral stroke after transient ischemic attacks (TIAs).Methods The clinical data of 184 TIA patients were collected.The risk factors of ischemic cerebral stroke after TIA were analyzed.Results Compared with no ischemic cerebral infarction group, the rate of elderly (≥60 years old), hypertension, diabetes, smoking and drinking history in ischemic cerebral infarction group (P<0.05-0.01).There was no statistical significance in gender and hyperlipidemia between the two groups (all P>0.05).Compared with no ischemic cerebral infarction group, the rate of attack time≥30 min, attack frequency≥3, course of disease≥24 h were significantly increased (P<0.05-0.01).There was no statistical significance in TIA types between the two groups (all P>0.05).Logistic regression analysis showed there were a positive correlations between elderly, hypertension, diabetes, smoking and drinking history and ischemic cerebral stroke after TIA (OR=29.799, 95%CI: 2.189-405.569, P=0.011;OR=0.649, 95%CI: 0.038-6.850, P=0.005;OR=8.569, 95%CI: 1.314-55.862, P=0.025;OR=0.158, 95%CI: 0.025-0.980, P=0.048).Conclusion Elderly, hypertension, diabetes, smoking and drinking history are independent risk factors for ischemic cerebral stroke after TIA.

Objective To evaluate the effect of Liujunzi soup on the total course of disease and inflammatory mediators in patients with remission of allergic cough. Methods Eighty six patients with allergic cough were enrolled in our hospital from April 2011 to April 2012. The patients were randomly divided into observation group (n = 43) and control group (n = 43). The control group was given basic western medicine treatment, the observation group in the control group on the basis of another treatment of six gentleman soup. The levels of inflammatory cytokines, pulmonary function and adverse reactions were compared between the two groups in clinical efficacy, hospitalization time, total course of disease, IL-2, TNF-α and IL-6. Results The total effective rate was 90.70％ in the observation group was significantly higher than that in the control group (72.09％)( P<0.05). The duration of hospitalization (13.35 ± 3.24) d and the total course of disease (17.68 ± 4.53) d were significantly shorter than those in the control group (16.23 ±3.10) d, (20.35 ± 4.67) d (P<0.05). The levels of inflammatory factors and pulmonary function in the observation group were statistically significant compared with the control group (P<0.05). No adverse reactions occurred in both groups. Conclusion Liujunzi Decoction combined with western medicine can improve the level of inflammatory cytokines and lung function, shorten the length of hospital stay, the total course of disease and high safety, and have good clinical value.

Astragaloside Ⅳ content in species of Radix Astragali seu Hedysari of Chinese materia mediea has been determined with dual wavelength,reflect zig-zag scanning,external-standard dual-point methods. The average recovery and coefficient of variation(CV)are 99.15% and 3.71%(n=6),respective- ly.

Asthma, a complex polygenetic disease, has individual differences in patient′s response to asthma drugs,which is mainly related to gene polymorphism. These genes include beta 2-adrenaline receptor genes(ADRB2),the glucocorticoid receptor genes(NR3C1) and leukotriene synthase genes(LTC4S and ALOX5),etc. Pharmacogenomics can realize individualized treatment of asthma,reduce the admission rate and the mortality rate,and reduce the cost of treatment. Pharmacogenomics of children asthma is reviewed in this arti-cle,hoping to provide help for the individualized treatment of asthma.