Objective To develop a kind of clinical nursing human resource mamagement basic data set fits to national and international standard. Methods CNHRMBDS was developed according to refereces and related standard, and then compared with related data set of nursing management. Results There were 179 data elements in the CNHRMBDS. It included all data elements related to human resources for health in China Hospital Basic Data Set Standard 1.0Version, China Public Health Information Classification and Basic Data Set Standard1.0 Version, and Community Health Information Basic Data Set Standard. Twenty-four data elements in the World Health Organization Human Resources for Health Minimum Data Set and 155 data elements in the American Nursing Management Minimum Data Set were found comparable to those in the CNHRMBDS. Conclusions The CNHRMBDS was found complete and able to meet relevant national and in-ternational standards. It should be further developed to be a national standard for health profession.

Objective To investigate the relationship between plasma concentration of osteopontin (OPN) and disease activity in systemic lupus erythematosus (SLE).Methods The plasma concentration of OPN in 28 healthy controls and 38 SLE patients was measured by enzyme-linked immunosorbent assay. Results The plasma concentration of OPN was significantly higher in SLE patients compared to that in the healthy controls (420?203 ng/mL vs 73?14 ng/mL,P＜0.001),in the patients with proteinuria com- pared to that in the patients without proteinuria (623?88 ng/mL vs 288?135 ng/mL,P＜0.001),in the patients with active disease compared to that in those with inactive disease (529?143 ng/mL vs 185?66ng/mL,P＜0.001),in the patients before treatment compared to that in the patients after treatment (556?130 ng/mL vs 142?22 ng/mL,P＜0.001).The plasma concentration of OPN in patients with arthritis was not different from those without arthritis (489?153 ng/mL vs 375?222 ng/mL,P＞0.05).Plasma concentration of OPN showed a significant positive correlation with systemic lupus erythematosus disease ac- tivity index (SLEDAI) (r=0.93,P＜0.001),but a significant negative correlation with serum C3 level (r=-0.49,P＜0.05).There was no correlation between plasma concentration of OPN and antinuclear an- tibody titre,erythrocyte sedimentation rate,or serum level of C4.Conclusions These data show that plas- ma OPN level has some correlation with the SLE activity,renal damage and progression or amelioration of SLE.OPN could be involved in the pathogenesis of SLE.

Objective To observe the efficacy of acupuncture plus medication in treating diarrhea-irritable bowel syndrome (IBS-D) due to liver depression and spleen deficiency, and its effect on serum 5-hydroxytryptamine (5-HT), calcitonin gene-related peptide (CGRP) and endothelin (ET). Method Sixty IBS-D (liver depression and spleen deficiency) were recruited and randomized into an observation group and a control group, 30 cases each. Patients in the observation group were intervened by acupuncture plus modified Bai Zhu Shao Yao powder, while the control group was intervened by acupuncture alone. The total effective rate, change of syndrome score of traditional Chinese medicine (TCM) and diarrhea-irritable bowel syndrome quality of life scale (IBS-QOL), as well as the levels of serum 5-HT, CGRP and ET, were observed. Result The total effective rate was 83.3% in the observation group, significantlyhigher than 63.3% in the control group (P<0.05). There were no significant differences in comparing the global and component TCM symptoms scores and IBS-QOL between the two groups before the treatment (P>0.05). The global and component TCM symptoms scores and IBS-QOL dropped significantly in both groups after the intervention (P<0.05). The global TCM symptoms score and IBS-QOL in the observation group were significantly lower than those in the control group after the intervention and 3 months after the intervention (P<0.05); the component TCM symptoms scores in the observation group were significantly lower than those in the control group after the intervention (P<0.05). There were no significant differences in comparing the serum 5-HT, CGRP and ET levels between the two groups (P>0.05) before the treatment. The levels of 5-HT, CGRP and ET decreased significantly after the treatment in both groups (P<0.05); after the intervention, the levels of 5-HT, CGRP and ET in the observation group were significantly lower than those in the control group (P<0.05). Conclusion Acupuncture plus medication can effectively mitigate the symptoms in IBS-D (liver depression and spleen deficiency) and enhance the quality of life, which is possibly achieved by down-regulating the serum 5-HT, CGRP and ET levels.

Objective:To investigate the vasorelaxant effect of active fraction from compound Prunella vulgaris L. ( AFCP) on the i-solated thoracic aorta of rats and underlying mechanism. Methods:The study was performed with the tension experiment of the isolate rat thoracic aorta. The changes of vascular ring tension were measured by biological signal acquisition and analysis system, and the vasodila-tor effect of AFCP was observed. Results:AFCP(100-500μg·ml-1)could induce significant relaxation in aorta rings pre-contracted by phenylephrine (PE,1 μmol·L-1), and the relaxation effect was significant(75% ±8%) in endothelium-intact aortic and endothelium-denuded aortic. The vasodilatation effect of AFCP was not significantly affected by nitric oxide synthase ( NOS) inhibitor NG-nitro-L-argi-nine( L-NNA) , guanylate cyclase inhibitor MB,potassium channel blocker TEA and glibenclamide. In Ca2+-free bath solutions, AFCP (300 μg·ml-1 ) could shift downward dose-response curve of CaCl2 and significantly reduce the maximum contraction amplitude of PE. Conclusion:AFCP can relax rat aorta rings in a dose-dependent manner, which is endothelium-independent. The mechanism may be re-lated to the inhibition of intracellular calcium release and extracellular calcium flow, and has nothing to do with NO pathway, prostacyclin generation and calcium-activated potassium channels.

Objective To investigate the effects of Astragalus Polysaccharide (for intravenous injection) in stimulating the secretion of hematopoietic growth factors from activated human peripheral blood mononuclear cells (PBMC). Methods Human PBMC were isolated from concentrated leukocytes and were cultured with Astragalus Polysaccharide. Hematopoietic growth factors such as granulocyte colony-stimulating factor (G-CSF) and granulocyte-macrophage colony-stimulating factor (GM-CSF) in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA). Results Astragalus Polysaccharide stimulated G-CSF and GM-CSF formation in a dose-dependent and a dose-effect and time-dependent manner. Conclusion Astragalus Polysaccharide can stimulate the secretion of hematopoietic growth factors from activated human PBMC, indicating its clinical application for leukocytopoiesis.

Objective The article was to speed up the dissolution rate of poorly soluble drugs, reduce the configuration time and improve the allocative efficiency by analysis on factors influencing dissolution and adoption of proper allocative approaches accord-ing to different characteristics of insaluble drugs. Methods 5 insaluable drugs were chosen for the study, which were ornithine as-partate, mezlocillin sodium and sulbactam sodium, teicoplanin, omeprazole sodium injection and imipenem and cilastatin sodium.The control group were allocated by routine configuration approach, while the experiment group were improved by increasing the amount of solvent, loosing the powder inside the bottle before configuration, reducing the bubble, adopting special solvent and increasing the dis-solution temperature.Observation and comparative experiment were made on two groups along with the timing from configuration to com-plete dissolution into clear transparent liquid. Results As to the average complete dissolution time of 5 drugs, significant difference was found between two groups([42±5]s vs [246±35]s, [3±1]min vs [30±3]min, [5±1]min vs [10±3]min, [5±3]min vs [10± 5]min, [2±1]min vs [10±1]min, P<0.05).Compared with the control group, the dissolution time of 5 drugs was cut down by 83%, 90%, 50%, 50%, 80%in experiment group. Conclusion Appropriate increase of solvent, loose powder, bubble reduction, special solvent and increased solution temperature contribute to shortening the dissolution time of insoluble drugs and improving the configura-tion efficiency of pharmacy intravenous admixture.

Objective To evaluate the performance of self‐established angiotensin converting enzyme (ACE) detection system . Methods The performance evaluation of ACE reagent kit produced by the Jiuqiang Company including precision ,linearity ,correla‐tion with the reference reagent and reportable range were conducted by using the automatic biochemical analyzer according to the re‐quirements of the EP5‐A2 and EP9‐A documentation in the Clinical and Laboratory Standards Institute (CLSI) .Results Intra‐as‐say CV of the system were 6 .87% ,2 .39% and inter‐assay CV were 6 .09% ,1 .81% ,respectively .During the day CV were 8 .00 %and 2 .8% respectively ,which were less than those provided by the manufacturer (<10% );the lenearity result was R2 =0 .99 .The correlation coefficient (r) of the system comparing with the reference reagent was 0 .990 56 ,moreover the average bias was 8 .55% , showing good correlation ;the repotable range was 9 .0U/L‐600U/L .Conclusion Self‐established ACE detection system can meet the requirements for clinical application .

Objective To observe the effect of elderly patients with gallstones by surgery combined with Xiaoyanlidan tablets in the treatment of the individualized nursing intervention effect of health and living habits of patients based on. Methods 94 elderly patients with gallstones were treated surgically in our hospital were randomly divided into control group (n=47) and study group (n=47), the two groups of patients after all give Xiaoyanlidanpian oral treatment, the control of 47 cases of elderly patients with basic nursing group on the basis of this, the study group was given 47 cases of elderly patients with individualized nursing intervention on the basis of the control group. Evaluation of two groups before and after nursing care in elderly patients with negative emotions and quality of life changes, after the concept of nursing Observe two groups of elderly patients with rehabilitation and nursing effect. Results The implementation of clinical intervention and nursing care of two groups after SDS and SAS scores were significantly reduced and the difference before and after group (P<0.05), and the patients in the study group, SDS and SAS score decreased to a greater degree (P<0.05); the two group patients before treatment GIQLI score was significantly lower, GIQLI scores of the two groups increased significantly after the implementation of nursing, compared before and after nursing group (P<0.05), and the study group the GIQLI score increased to a greater degree, the difference between the two groups was statistically significant (P<0.05); the control group and the study group of patients with Kang Fuxian The efficiency were 85.11% and 97.87%, the difference between the two groups was statistically significant (P<0.05); the control group and the study group patients nursing satisfaction rate were 61.70% and 89.36%, the difference between the two groups was statistically significant (P<0.05). Conclusion The health and living habits of patients based on the individualized nursing intervention is helpful to improve the curative effect in the treatment of old patients with gallstones taken surgery combined with Xiaoyanlidan tablets, significantly improve the depression and anxiety status of the patients, it has important significance to restore and improve the quality of life of patients after operation.

Objective To observe the effect of angiotensin-converting enzyme inhibitor(ACEI) on insulin sensiti-(vity) in dogs with acute myocardial infarction(AMI) after thrombolytic treatment. Methods Twenty dogs were made as AMI models and then underwent thrombolytic treatment.The dogs were divided into the control group(n=10) and the captopril group(n=10) randomly.Insulin,plasma glucose,erythrocyte insulin receptor(EIR),nitrogen(NO) and angiotensin Ⅱ(AT Ⅱ) were(detected) and insulin sensitivity index(ISI) was calculated.The changes of these values in the two groups were contrasted. Results After reperfusion for 120 min,in the control group,ISI and AT Ⅱgot much more rise while EIR and NO fell much more(P

0.05).At the eighth week of training and after ceasing the cognitive training for 4 weeks the NCSE scores and the FIM scores were improved in both groups,espeeially in the cognitive training group(P