OBJECTIVE:To compare economical efficiency of 4 therapeutic regimens for pediatric perianal abscess. METH-ODS:By retrospective study method,85 children were divided into group A(18 cases),B(10 cases),C(14 cases)and D(43 cases)according to different therapeutic regimens. They were given amoxicillin-flucloxacillin,flucloxacillin,cefathiamidine and ce-famandole 100 mg/(kg·d) intravenously for 5-7 d,respectively. Clinical efficacies and the occurrence of ADR were compared among 4 groups. The economical efficiency of 4 therapeutic regimens were analyzed by the method of cost-effectiveness analysis. RESULTS:For group A,B,C and D,the cure rates were 77.78%,80.00%,57.14% and 90.70%,that of group C was signifi-cantly lower than those of other 3 groups,with statistical significance(P<0.05). No significant ADR were found in 4 groups. The costs were 3 600.86,2 913.92,3 416.19 and 2 981.97 yuan,and the cost-effectiveness ratios were 46.30,36.42,59.79 and 32.88. The incremental cost-effectiveness ratios of group A,C and D to group B were -309.43,-21.97 and 6.36. The results of cost-ef-fectiveness analysis were supported by sensitivity analysis. CONCLUSIONS:For pediatric perianal abscess,cefamandole shows the best cost-effectiveness;but the therapeutic duration and total cost of flucloxacillin therapeutic regimen is shorter and lower. Thera-peutic regimen should be selected according to children’s condition.

Objective To explore the relationship between self-confident and personality of college students' self-confident.Methods 527 college students were investigated with Personal Evaluation Inventory 16.04)than that of girls(141.13±15.76,t=2.558,P＜0.05);the score of students from urban areas was higher(145.14±15.66)than that of students from rural areas(141.54 ±15.99,t=2.394,P＜0.05);the score of students from one-child family was higher(146.50±16.76)than that of students from non-only-child family (141.57±15.61,t=2.910,P＜0.01);the score of students from Different Grades existed significant differences students(r=0.548,P＜0.01),and it was significantly negative correlation with neuroticism(r=-0.207,P＜0.01)and psychoticism(r=-0.476,P＜0.01).Conclusion There are significant difference of gender,family locus,only-child whether or not and grade in self-confident of college students;and self-confident of college students also significantly correlated with personality characteristics.

Objective] Research the treatment characteristics of premenstrual abdominal pain and postmenstrual abdominal pain in Fu Qingzhu’s Obstetrics and Gynecology and the clinical medical records.[Method] By reading the relevant documents and medical records to discuss the medication characteristics of premenstrual abdominal pain and postmenstrual abdominal pain in Fu Qingzhu’s Obstetrics and Gynecology along with the medication experience of dysmenorrhea from the traditional Chinese medicine expert Guo Zhenqing. [Results] Fu Qingzhu treated dysmenorrhea by distinguishing between the anterior and posterior with different treatments such as Xuanyu Tongjing decoction and the decoction for regulating liver. Focus on liver qi stagnation and use of white peony root, angelica nourishing blood and liver to relieve pain .Old Guo paid attention to treatment based on syndrome differentiation and modified acupoint prescriptions based on Xuanyu Tongjing Decoction. She treated dysmenorrhea with two prescriptions, two periods of medication and hit the pathogenesis. Achieve satisfactory results in the treatment of this disease. [Conclusion] The clinical dysmenorrhea syndrome is complex, Xuanyu Tongjing Decoction and the decoction for regulating liver should not be confined to the treatment before and after abdominal pain, must have detailed exploration of its thought and use the original prescription flexibly, which can be effective.

Objective:Peripherally inserted central catheter(PICC) has been application in clinical for many years,but the training in the knowledge about maintenance wasn't univesal systematically yet.To analyze the existed problems on the clinical peripherally inserted central catheter in washpipe technology and find out countermeasures. Methods: The self-made questionnaire was used to survey a total of 46 nurses in every department and make on-site operation demonstration. Results: Only 12 nurses' operation was completely correct and the correct rate was 26.1percent,which was related to the training,manager's attention,nurses' participate awareness. Conclusion: The training on nurses' operation of red tube needed to be strengthen.

Through the comparative analysis of traditional medical dispute handling methods and the concept of the third party mediation in medical dispute,this paper discussed that the third party mediation method in medical dispute obtain the recognition of both doctor and patient with its advantages such as neutrality,impartiality,convenience,and it had good running effect in the practice from place to place.But there still remain Some Problems,in cluding the intervention lacks initiative;the amount of indemnity lacks scientificity;the conciliation agreementlacks coercive enforcement;the privacy lacks protection;the institution development is limited.So we must improve the third party mediation work mechanism,identification process,operation mechanism and the relevant insurance system.

Objective To observe the influence of modified Sijunzi Decoction on the decrease of immune function of exercise-induced exhaustion rats. Methods Thirty SD rats were given adaptive training, and then 6 dead or non-adaptive rats were dropped out. The remaining 24 SD rats were randomly divided into normal group ( N= 8) , model group ( N = 8) , and modified Sijunzi Decoction group ( MSD group, N = 8) . Normal group did not receive any medication and took diet freely. The rats of model group and MSD group were orally given distilled water or modified Sijunzi Decoction 8 g per day respectively after the modeling by exercise-induced exhaustion. After 61 days, the serum was taken from the three groups, and immunoglobulin lgA, lgG, lgM, and complement C3,C4 were detected. Results The contents of immunoglobulins IgA, IgG, IgM and complements C3, C4 in MSD group were higher than those in the model group ( P<0.01) . Conclusion The decrease of immunity occurs in the process of exercise stress, but can be delayed by modified Sijunzi Decoction..

To investigate the cell proliferation inhibition and apoptosis induced by berberine a-hydroxy f-decanoylethyl sulfonate (HB) on MDA-MB-231 cells in vitro, and the inhibitory effect of HB on the expression of poly adenosine diphosphate RNA polymerase (PARP), MTT assay was used to detect the viability of MDA-MB-231 cells and cell cycle was examined by flow cytometry. The results showed that HB could significantly inhibit the proliferation of MDA-MB-231 cells, and mildly arrested cell cycle progression at S phase. The IC50S for 24, 48 and 72 h treatment were 4.65, 1.46 and 0.75 mg.L-1 (7.55, 2.37 and 1.22 micromol.L-1), respectively. Annexin V-FITC/PI double staining assay showed that HB increased apoptotic ratio of MDA-MB-231 cells. Western blotting analysis showed the expressions of procaspase-3, procaspase-9 and PARP were decreased after HB treatment, while their fragment increased. The results suggest that HB can inhibit the growth and induce apoptosis of MDA-MB-231 cells, which may be associated with inhibition of the expression of procaspase-3, procaspase-9 and PARP.

With the development of mass spectrometry technology and its wide range of applications in the biological field,Veristrat classifier has been applied in non-small cell lung cancer (NSCLC) individualized therapy as well as in other tumors.Oncologists select individualized treatment plan based on peripheral blood veristrat test results.Many trials have verified its guiding role in NSCLC and other tumors' s first line and multi-line individualized therapy.Veristrat classifier provides a new idea and method for individualized treatment of NSCLC and other tumors.

Synergistic antitumor effects of HB (berberine alpha-hydroxy-beta-decanoylethyl sulfonate, houttuyn berberine) with HCPT (hydroxycamptothecine), and its correlative mechanism were studied in vitro. MTT assay was employed to determine the cytotoxicity of HB combined with HCPT in tumor cells culture in vitro, IC50 and combination index (CI value) were used to evaluate the synergistic effects. The supercoiled DNA relaxation mediated by topoisomerase I & II was measured by agarose gel electrophoresis assay, and influence of HB was detected. The results showed that HB could inhibit the proliferation of tumor cells (SGC-7901, SW1116 and SW480) in vitro, and the inhibition ratio was increased, IC50 was reduced when combining with HCPT. CI value of the two drugs was less than 1 in HepG2, SW480, SGC-7901 and SW1116 cells. The lowest value was 0.447, 0.626, 0.161 and 0.178 in these tumor cells, respectively, further indicating HB has synergistic action with HCPT on suppressing tumor proliferation. The agarose gel electrophoresis assay showed HB can inhibit topoisomerase I & II activity of SW480 cells at the concentration of 2.0-8.0 mg x L(-1). HCPT is a typical inhibitor of topoisomerase I , the synergistic action between HCPT and HB on suppressing tumor proliferation is perhaps related to the congenerous inhibition of topoisomerase.

Objective To investigate whether and how regulatory T cells(Tr) affect macrophages foam-cell formation, and thereby investigate the mechanism of Tr in the development of atherosclerosis. Methods Tr were isolated from lymphocyte suspensions by magnetic cell sorting-column and analyzed by flow cytometry. Macrophages were cultured alone, with CD4~+ CD25~+ T, or CD4~+ CD25~- T cells in the pres-ence of oxLDL for 48 h to transform macrophages into foam cells. Oil red O staining and cellular lipid meas-urement were used to identify macrophage foam cell formation. Semi-quantitative PCR, quantitative real-time PCR and Western blot analysis were carried out to explore the mechanism of Tr-mediated suppression on macrophages foam cell formation. Results Foam cell formation, as identified by oil red O staining, was readily apparent in cells treated with CD4~+ CD25~- T cells and without T cells. After treatment with Tr, a marked decrease(13.9% ± 5.6% ) in foam cell count was observed, compared with untreated cells(13.9% ±5.6% vs 52.9% ± 10.4%, P<0.001 ) or CD4~+ CD25~- T-treated cells(13.9% ± 5.6% vs 53. 1% ± 17.2%, P<0.001 ), 52.9% ± 10.4% and 53.1% ± 17.2%, respectively (P<0.001). The similar effect of Tr was obtained when extracted oil red O and measured by a spectrophotometer. Cholesteryl ester ac-cumulation also used to quantify foam cell formation. Compared with untreated (no T) and CD4~+ CD25~- Tr-treated macrophage cells (CD25~-), the lipids accumulation in CD4~+ CD25~+ Tr-treated macrophage foam cells(CD25~+) was significantly reduced. Total cellular cholesterol and cellular cholesteryl ester was siginifi-cantly reduced in CD25~+ cultures relative to no T[TC(total cholesterol): 57.46 ± 17.92 vs 159.48 ± 16.38, P<0.01 ; CE(esterified cholesterol): 26.68 ± 8.88 vs 102.54 ± 16.67, P<0.001] or CD25~- (TC: 58.50±7.00 vs 150.55±25.11, P<0.01; CE: 26.68±8.88 vs96.90 ± 11.95, P<0.001) cul-tures. Moreover, PCR and Western blot analysis showed that the expression of both CD36 and SRA in Tr-treated macrophage foam cells was significantly down-regulated. Conclusion Results collectively suggest that CD4~+ CD25~+ Tr cells may inhibit macrophage foam-cell formation, which is largely attributed to a down-regulated expression in scavenger receptor in Tr-treated macrophage foam cells.