1.Effect of high glucose on the expression of activating transcription factor 4 in cultured retinal Müller glia cells
Chinese Journal of Ocular Fundus Diseases 2012;28(5):498-500
Objective To observe the effect of high glucose on the expression of activating transcription factor 4 (ATF4) in cultured retinal Müller glia cells.Methods The retinal tissue of Sprague Dawley (SD) rats was collected,and Müller cells were isolated and cultured.The glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS) of Müller cells were identified by streptavidin-biotinperoxidase complex.Cultured rat Müller cells were divided into control group (5.5 mmol/L glucose),group A (20 mmol/L glucose),group B (30 mmol/L glucose) and group C (40 mmol/L glucose).ATF4 protein expressions in Müller cells of four groups were measured by Western blot four days after cultured.Results GFAP and GS expressed in more than 95% of Müller cells.Over 95% of Müller cells of group A,B and C were positive for GFAP and GS.Western blots indicated that ATF4 protein in group A,B and C increased obviously compared with the control group (q=0.293,0.754,0.484; P<0.05).Conclusion High glucose can increase the expression of ATF4 protein and cause endoplasmic reticulum stress in retinal Müller glia cells in vitro.
2.Risk factors analysis of postoperative intracranial infection in patients with recurrent glioma.
Chinese Medical Journal 2013;126(9):1796-1796
Brain Diseases
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etiology
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Brain Neoplasms
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surgery
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Glioma
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surgery
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Humans
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Infection
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etiology
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Postoperative Complications
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etiology
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Recurrence
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Risk Factors
3.Livedoid vasculitis: an update
Chinese Journal of Dermatology 2016;49(5):371-374
Livedoid vasculitis is a kind of skin disease caused by local vascular occlusion.It mainly manifests as erythematous and purpuric maculas or papules on the legs and ankles,which may progress into intensely painful ulcers and heal leaving porcelain-white atrophic scars called atrophie blanche.Histopathologically,inflammatory response is weak or absent in local lesions of livedoid vasculitis,hence,it is commonly considered to be related to local thrombosis and other autoimmune diseases.Now,it is mainly managed with anticoagulation-based comprehensive treatment in clinic.
4.Meta analysis of non-penetrating trabecular surgery versus trabeculectomy for the treatment of open angle glaucoma.
Min, KE ; Jing, GUO ; Zhigang, QIAN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2011;31(2):264-70
This study compared the efficacy of non-penetrating trabecular surgery and trabeculectomy for the treatment of open angle glaucoma. We searched the Cochrane Library, PUBMED (1966 to 2009), Embase (1980 to 2009) and CMB-disk (1979 to 2009) for the randomized clinical trials (RCT) concerning the two treatment strategies. The reports, including the papers listed in bibliographies, were evaluated against a set of quality criteria and the RCTs that satisfied the criteria were selected and subjected to Meta analysis by employing the Cochrane Collaboration's RevMan 4.5 software package. A total of nine RCTs were included in the study. The analyses of the reports showed that, 12 months after surgery, there was significant difference in the reduction of interocular pressure (IOP) between non-penetrating trabecular surgery and trabeculectomy (Z=6.05 P<0.00001). There also existed statistically significant difference in the reduction of IOP at the censored time between the two procedures (Z=4.92, P<0.00001). Difference in the success rate was also found between the two surgeries (Z=3.82, P=0.0001). It is concluded that, compared with the non-penetrating trabeculectomy, the traditional trabeculectomy could reduce IOP more and had higher success rate while the non-penetrating trabecular surgery is associated with lower postoperative complications.
5.Effect of high glucose on cultured retinal Müller cell in vitro
Jing, GUO ; Min, KE ; Xiao-feng, WEN
Chinese Journal of Experimental Ophthalmology 2012;30(9):791-794
Background Retinal Müller cells can offer nutrient and maintain the normal structure of retina.Researches showed that the abnormality of Müiller cells leads to retinal vascular disease.To explore the effect of high glaucoma on retinal Müller cells is of a very important significance for the study on diabetic retinopathy (DR).Objective This study was to investigate the effects of different concentrations of glucose on retinal Müller cells in vitro.Methods Retinal tissue was isolated from 1 10-day-oM clean SD rat.Mtiller cells were cultured by explant culture method and passaged in DMEM containing 20% fetal bovine serum.The third generation of cells were obtained and identified using glial fibrillary acidic protein (GFAP) staning.Then,5.5,30.0 and 40.0 mmol/L glucose were added into the culture medium for 4 days respectively.The proliferation (A570) of Müller cells was detected by MTT,and apoptosis rate of Müller cells was calculated by flow cytometer to evaluate the effects of 5.5,30.0 and 40.0 mmol/L glucose to cell vitality.Results Cultured and passaged cells grew well with the spindle shape.The positive reactive cells were >95% for GFAP.The A570 value of Müller cells was 0.24±0.01,0.21±0.03 and 0.20±0.02 in 5.5,30.0 and 40.0 mmol/L glucose group respectively,showing a significant difference among the three groups(F=6.755,P<0.05).Compared with 5.5 mmol/L glucose group,As70 values were significantly lower in 30.0and 40.0 mmol/L glucose group (q =0.645,0.486,P < 0.05).Apoptosis rates of Miiller cells were (26.40 ±0.25)%,(30.19±0.16)% and (36.23±0.19)% in 5.5,30.0 and 40.0mmol/L glucose groups,with a significant difference among them (F =294.530,P<0.05),and those in 30.0 and 40.0 mmol/L glucose groups were significantly reduced in comparison with 40.0 mmol/L glucose group (q =0.754,0.484,P < 0.05).Conclusions High concentration of glucose inhibits the viability and promote the apoptosis of retinal Müller cells at a concentrationdependent manner.
6.Application value of the jejunal exclusion length in gastric bypass for obesity and type 2 diabetes mellitus
Weidong TONG ; Zhigang KE ; Jing CHEN
Chinese Journal of Digestive Surgery 2017;16(6):555-558
Roux-en-Y gastric bypass (RYGB) is the preferred surgical procedure for obesity and type 2 diabetes mellitus (T2DM).Currently,it is controversial how long the roux limb and biliopancreatic limb should be selected during RYGB.Based on the survey of American Society for Bariatric Surgery (ASBS) (2008),the Roux limb ranged from 10 cm to 250 cm and the biliopancreatic limb ranged from 35 cm to 250 cm.The length of small bowel exclusion may affect the postoperative results.Shorter jejunum exclusion may affect the curative effect,while overlong jejunum exclusion may result in nutritional disorders,diarrhea and other complications.Under the condition of fixed jejunal exclusion,it is worth exploring the relationship between the postoperative outcome and different parameters of small bowel exclusion.Lacking of consensus and criteria in this field,authors focused on the related references published recent years to summarize the valuable information.
7.Association of Gly82Ser polymorphism of the RAGE gene with pathogenesis of colon cancer in Chinese population
Fei QIAN ; Wenyi ZHANG ; Jing KE
Chinese Journal of General Surgery 2013;(6):444-447
Objective To investigate the role of Gly82Ser polymorphism of receptor for advanced glycation end-products (RAGE) in the genesis and progression of colon cancer in Chinese population.Methods Using the method of PCR-restriction fragment length polymorphism (PCR-RFLP),the Gly82Ser genotype of RAGE were examined in 90 colon cancer patients and 78 control subjects age and sex matched.Analyses stratified by TNM and tumor differentiation were conducted to check the associations of the Gly82Ser gene polymorphisms in RAGE and development of colon cancer.Results The genotype distribution was in agreement with that predicted under Hardy-Weinberg equilibrium for both patients and controls (both P > 0.05).With the GG genotype as reference,the odds ratio (OR) for heterozygous GG and carriers with S allele (GS and SS) were 2.037 (95% CI:1.207-3.438) and 2.022 (95% CI:1.275-3.208),respectively,which had a significantly higher risk of colon cancer.Moreover,the elevated colon cancer risk was especially evident in patients with TNM (Ⅲ + Ⅳ) and/or patients with poor differentiation by stratification analysis (OR,3.575,95% CI:1.495-8.550 and OR,3.580,95% CI:1.390-9.217,respectively).Conclusions The RAGE Gly82Ser polymorphism may confer not only an increased risk of colon cancer but also with invasion of colon cancer in the Chinese population.
8.Research progress in the biology of Hodgkin's lymphoma in the 54th ASH annual meeting
Wei ZHANG ; Jing WANG ; Xiaoyan KE
Journal of Leukemia & Lymphoma 2013;22(7):385-386
The Hodgkin and Reed/Sternberg (HRS) tumor cells of classical Hodgkin's lymphoma (HL) and the lymphocyte-predominant (LP) tumor cells of nodular lymphocyte-predominant HL are both derived from germinal center B cells.Several studies show that HL pathogenesis includes the deregulated activity of numerous members and regulators of the NF-κB and JAK/STAT signaling pathways,genetic lesions of epigenetic regulators,and the interactions between tumor cells and other infiltrating cells in the microenvironment,et al.
9.Primary mediastinal lymphoma: reports from the 19th European Hematology Association annual congress
Yifan PANG ; Jing WANG ; Xiaoyan KE
Journal of Leukemia & Lymphoma 2014;23(10):589-592
Primary mediastinal large B-cell lymphoma (PMBCL) is a unique type of B-cell lymphoma probably arising from a putative thymic medulla B cell.It constitutes 6 %-10 % of all diffuse large B-cell lymphomas (DLBCL),with special pathologic features,immunophenotype and genetic abnormlities,and need to be identified with classical Hodgkin lymphoma and other types of DLBCL.In this review,the pathological features,molecular biological changes,treatment,prognosis and treatment of PET in the evaluation of the initial reaction in PMBCL were summarized.
10.Advances in biological prognostic indicators of chronic lymphocytic leukemia
Lan MA ; Jing WANG ; Xiaoyan KE
Journal of Leukemia & Lymphoma 2010;19(1):55-58
The patients of chronic lymphocytic leukemia(CLL)have great individual differences.It is important for clinicians to determine the prognosis at the beginning of diagnosis and choose proper treatment for the patients.The recent advances in biological prognostic indicators of CLL were reviewed,including IgVH gene mutation status,ZAP70,CD_(38),chromosome abnormality[t(11q;v),del(11q),del(17p),+12 and del(13q)].telomere and telomerase.