Objective To investigate the feasibility,technique and clinical significance of preserving the great auricular nerve in parotid benign tumors surgery during the first postoperative year.Methods Fifty-two patients with parotid benign tumors were randomly divded into 2 groups.24 patients (group sacrificed) underwent classic parotidectomy with sacrifice of the great auricular nerve.The surgeons spare the nerve in the 32 patients with FOCUS ultrasonic harmonic scalpel (group preserved).Results After surgery,both groups showed lower levels of subjective sensation and sensory function test at auricle.These alterations were less pronounced in group preserved.Both groups showed improvement over time.In group preserved the sensory function reached normal level by 12 months after surgery.The recuperation in group sacrificed was partial at 12 months after operation.Conclusion The great auricular nerve preservation during parotidectomy is feasible,It can decrease sensory disturbance in the early postoperative period and avoid the permanent sequelae that occur when the nerve is sacrificed.The FOCUS ultrasonic harmonic scalpel technique can reduce the operation time.

Objective To investigate the changes in proliferation index (PrI) of gingival fibroblasts in nifedipine-induced gingival hyperplasia ( NIFr-HGF). Methods Gingival fibroblasts were derived from a patient with nifedipine-induced gingival hyperplasia. Cells were induced by 10 ng/mL and 1 000 ng/mL nifedipine ( low- and high-concentration drug intervention groups), respectively. Cells were harvested 18 h and 30 h after intervention, cell cycles were detected by flow cytometry, and Prls were calculated. NIFr-HGF without nifedipine induction were served as blank control. Results After induction for the same time, Prls of NIFr-HGF cell cycle of low- and high-concentration drug intervention groups were significantly higher than those of blank control group (P <0.05) , while there was no significant difference between low and high-concentration drug intervention groups (P > 0.05). In low and high-concentration drug intervention groups, Prls of NIFr-HGF cell cycle after intervention for 30 h were significantly higher than those after intervention for 18 h [(57. 54 ± 0.019)% vs (21.15 ±0.011)%, and (59.36 ±0.031)% vs (19.01 ±0.012) %, respectively] (P < 0.05). Conclusion For patients with nifedipine-induced gingival hyperplasia, PrI of NIFr-HGF cell cycle increases with time of nifedipine intervention, while is not significantly related to drug concentration.

Papillary thyroid cancer (PTC) is the most common type in thyroid carcinomas.Recently,the prevalence and diagnostic rate of PTC has got significantly high with the general use of ultrasound.Nowadays,more and more studies have suggested that the coexistence of PTC and diabetes is common.They indicated that hyperglycemia would induce the deterioration of oxidative stress injury,chronic inflammation,insulin resistance,obesity,dyslipidemia,deficiency of vitamin D and dysimmunity.All of these may break the balance of oxidation and antioxidation and result in disordering signal pathway,accumulation of inflammatory cytokines,over activating of insulin and insulin-like growth factor-1,changing the metabolic pathways,which will promote the occurrence and progression of PTC.

Objective To explore the emotional socialization development between boys and girls aged 1-3 years. Methods A total of 1008 children were randomly selected with 571 boys and 437 girls. Chinese version of Urban Infant-Toddler Social and Emotional Assess-ment (CITSEA) was used to assess the emotional socialization development situation between boys and girls. Results There were statistical-ly significant differences between boys and girls in the overt behavior domain and ability domain of CITSEA (t>2.136, P<0.05), and was not in the covert behavior domain and imbalance domain (t<1.172, P>0.05). Conclusion There are gender differences in emotional socialization development of children.

Humans ; MicroRNAs ; Pulmonary Fibrosis

Objective To study the effect of thyroid-stimulating hormone (TSH) on human vascular endothelial cells and smooth muscle cells and to explore the roles of TSH in the development of atherosclerosis.Methods Human vascular endothelial cells and smooth muscle cells were cultured in vitro.MTT method was used to assay the effect of TSH on cell viability.Real-time PCR was used to determine the levels of endothelial nitric oxide synthase (eNOS),prostacyclin(PGI2),endothelin-1 (ET-1),plasminagen activator inhibitor(PAI-1) (mRNA) in endothelial cells and the phenotype transition of smooth muscle cells.The effect of TSH to the cycle of smooth muscle cells was detected by using flow cytometry.Immunocytochemistry and Western blot were used to investigate the expression of the cell cyclin A,D1,and the expression of endothelial cell associated factors eNOS and ET-1.Results Compared with the control group,eNOS and PGI2 mRNA levels decreased while ET-1 and PAI-1 mRNA levels increased when different concentrations of TSH were applied to endothelial cells(P＜0.05).The level of eNOS protein was decreased gradually while the level of ET-1 protein was gradually increased(P＜0.05).Different concentrations of TSH applied to smooth muscle cells could promote the transition of cell cycle phase G2 to phase M and increase the expression of cell cyclin A and D1.Conclusion TSH may damage the function of vascular endothelial cells and promote the proliferation of smooth muscle cells.

The expressions of HNF-4? and HNF-1? gene were assayed by RT-PCR in the livers of insulin-resistant rats and type 2 diabetic rats. mRNA levels of HNF-4? and HNF-1? in insulin-resistant rats were lower than those in control rats, and these levels were further decreased in diabetic rats. Changes in expressions of these 2 genes seem to be associated with type 2 diabetes mellitus.

Objective To assess myocardial ischemia in metabolic syndrome (MS), and risk factors of coronary heart disease (CHD) in MS, and to evaluate myocardial nuclein perfusion imaging (MPI). Methods 140 in-patients were divided into three groups according to the diseases they suffered from: metabolic syndrome (MS): 82 cases (m/f=54/28), essential hypertension (EH): 38 cases (m/f=15/23), and diabetes mellitus (DM): 20 cases (m/f=10/10). The degree of myocardial ischemia was detected by myocardial perfusion single-photon emission computed tomography (SPECT MPI). Results (1) The percentage of myocardial ischemia in MS group was 81.7%, and 56.8% of the patients in this group suffered from severe ischemia. Both percentages were higher than that in the other two groups (P

Abstract Objective:To investigate whether mRNA of prolactin(PRL) ,an important immunomodulatory hormone,is expressed in thyroidglands of patients with Graves'disease(GD) .Methods:The expression of PRL mRNA was examined by nonisotopic in situ hybridization in GDthyroid glands and the control ones( including multinodular goiters and samples of normal thyroid tissue adjacent to adenomas) as well as intra-thyroidal mononuclear cells isolated from GD thyroid glands and primary thyroid follicular cell(TFC) cultures derived from them.Results:PRL mRNA was expressed in GD thyroid glands, but not in the control ones. It was localized in infiltrating mononuclear cells and vascular endothelial cells adjacent to mononuclear cell infiltrates, but not in TFC .(9.8?.3)% (x ?s) of intrathyroidal mononuclear cells isolated from GD thy-roid were found containing PRL mRNA.It was absent in the primary TFC cultures. Conclusion:PRL mRNA is expressed in GD thyroid tissue. This indicates that PRL-like substance can be locally produced in GD thyroid tissue, which may play an important role in GD pathogenesis by autocrine and paracrine mechanisms.

Brown and white adipocytes have different metabolism characteristics.White adipocytes promote the formation and development of atherosclerosis.However, brown adipocytes contribute to the control of atherosclerosis.Brown and white adipocyte phe-notypes can transform mutually.This article will elaborate the pathogenesis of the transformation between brown/white adipocyte pheno-types regulated by transcription factors(such as PPARγ, PRDM16, PGC-1α), co-regulators, hormones, proteins, signal molecules, oxidative stress and miRNAs.