0.05),with the determination result of the whole blood-diluted sample more similar to that of the undiluated one.CONCLUSION:Whole blood sample of cyclosporine can be stored stably at room temperature at least for 2 weeks.Cold storage is the best storage method and blank whole blood was proved to be the best dilution solvent.

OBJECTIVE:To discuss the approach for clinical pharmacists to participate in the drug treatment.METHODS:The contents and importance for clinical pharmacist to participate in ward rounds and discuss with doctors about the optimal dosage regimen were introduced.RESULTS & CONCLUSION:A satisfactory outcome was able to be achieved through efforts of both clinical pharmacists and physicians to modify and work out a new individualized dosage regimen.Clinical pharmacists can offer direct,responsible drug-related pharmaceutical care for patients by making fully use of the related pharmaceutical knowledge they have mastered.

OBJECTIVE:To study the correlation between salivary drug concentration and blood,drug level its clinic sig-nificance.METHODS:The salivary drug concentration and blood drug level of gentamicin of15volunteer cases were deter-mined by radioimmunoassay.RESULTS:Both blood drug level and salivary drug concentration of gentamicin reached to peak level1hour after medication.CONCLUSION:The blood drug level of gentamicin and the salivary drug concentration of gentamicin are positively correlated,i.e.it is possible for salivary to substitute for blood in the therapeutic drug monitoring of gentamicin.

Objective To analyze the anesthesia effect of droperidol and pethidine hydrochloride in combina-tion with paracervical block in artificial abortion.Methods 386 patients undergoing artificial abortion were admitted. They were randomly divided into the two groups.The study group(193 cases)was treated with droperidol and pethi-dine hydrochloride in combination with paracervical block,while the control group (193 cases)was treated with droperidol and pethidine hydrochloride only.The analgesic effect,hemodynamics,cervix relaxation and side effects between the two groups were compared.Results The analgesic effect of the study group was 9 1 .7%,which was much higher than 72.0% of the control group(χ2 =25.198,P<0.01),and there were significant differences between the two groups in hemodynamics(t=4.545,3.674,all P<0.01).The total cervix relaxation were 102 cases,and partly cervix relaxation were 69 cases in the study group,which were significantly higher than that of the control group (69 cases,60 cases,respectively;Z=4.643,P<0.01).There was no difference between side effects except for abor-tion syndrome(χ2 =7.580,P=0.006).Conclusion Droperidol and pethidine hydrochloride in combination with paracervical block have an excellent effect on artificial abortion.

Objective:To investigate the principle of anesthetic management for intracranial aneurysm.Method:The anesthetic regimens of 34 cases of intracranial aneurysm were analyzed.Controlled lowering of blood pressure and protective measures for brain were implemented introperatively to prevent rapture of cerebral aneurysm and postoperative cerebrovascular spasm.Intraoperative ECG,MAP,HR,NIBP and SPO2 were monitored and the HR,MAP before and after induction phase were compare.Result:The effects of anesthesia and lowering of Bp were satisfactory,no death occurred intraoperative!y,and aneurysm ruptured m one case.The H-Rs of whole group during induction phase were not decreased as compared with those before induction phase,and the decrease of MAP was extremely significant as compared with that after induction phase.Conclusion:The effect on parts of brain should be considered when choosing narcotics and hypotensors.Perioperative brian protection could reduce the postoperative incidence rates of arterial spasm and cerebral infarction.

Humanities ; Knowledge ; Medicine

Cervical cancer is the most common malignancy of the female genital tract. Its incidence is still increasing with lower average onset age. Mass screening should be above prevention and treatment, and three screening programs, including the optimal program, the general program, and the basic program, are currently adopted in China. Cervical intraepithelial neoplasia (CIN) , a precancerous lesion, can be confirmed by the combined use of cytology, colposcopy, and histology and then managed with standardized approach. Human papillomavirus (HPV) infection is an essential factor during the development of cervical cancer, and persistent infection of high-risk HPVs may lead to CIN and subsequently develop to cervical cancer. High-risk HPV detection can be used for screening, differentiation of the atypical squamous cells of undetermined significance/ low-grade squamous intraepithelial lesion (ASCUS/LSIL) triage, and follow-up after treatment. The modern strategy of HPV infection is "to treat the disease, CIN, means to treat the virus, HPV". The licensing of HPV vaccine is an important event in cancer prevention, and this vaccine can be used for the primary prevention. However, early diagnosis and early treatment are still the most basic strategies for cervical cancer prevention and treatment.
Cervical Intraepithelial Neoplasia ; diagnosis ; therapy ; virology ; Early Diagnosis ; Female ; Humans ; Papillomavirus Infections ; diagnosis ; prevention & control ; therapy ; Papillomavirus Vaccines ; Uterine Cervical Neoplasms ; diagnosis ; prevention & control ; virology

Breast Neoplasms ; diagnosis ; therapy ; Cervical Intraepithelial Neoplasia ; diagnosis ; therapy ; Endometriosis ; diagnosis ; therapy ; Female ; Humans ; Neoplasms ; diagnosis ; economics ; therapy ; Ovarian Neoplasms ; diagnosis ; therapy ; Uterine Neoplasms ; diagnosis ; therapy

Objective To investigate the clinical characteristics of Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡ.Methods The clinical data of 1 case Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡwas retrospectively analyzed.Results The patient presented muscle weakness in proximal limbs, fasciculation, bulbar palsy, amyotrophy, postural tremor, sensory disturbance, gynaecomastia, impotency.The level of serum creatine kinase and urinary glutaric acid were elevated. Electromyogram and nerve and muscle biopsy supported mixed damage in peripheral nerves, as well as neurogenic and secondary myogenic pathologic changes in muscles.Gene sequencing indicated trinucleotide CAG repeated amplification for 47 times in chromosome X.Diabetes was diagnosed at 7 years after onset.Conclusions The pathogenesis of Kennedy disease is very complex, which may involve myogenic factor.The treatment to the secondary mitochondrial and lipid metabolic disturbance in muscle can improve the muscle weakness to a certain extent.

Objective: To detect the expression profile of NDRG1 gene in different tissues and cell lines and explore the relationship of NDRG 1 with liver tissue differentiation and hepatocarcinogenesis. Methods: The expression profiles of NDRG 1 in hepatocellular carcinoma (HCC) tissues, paired noncancerous liver (PNL) tissues, adult normal liver (NL) tissues, baby mouse liver tissues and fetal liver tissues in different developmental stages and cultured cell lines were performed using RT PCR and Northern Blot analysis. Results: Expression of NDRG 1 was significantly up regulated in HCC tissues compared to that of NL tissues and PNL tissues, however, the expressions of NDRG1 in NL and PNL tissues showed no evident difference. In ten cell lines, the highest expression was in the 293T human kidney cell line, the next one in liver cell L02, and the lowest one in the undifferentiated HLE cell line. Expression of NDRG1 in liver tissues enhanced with the development of the baby mouse and human fetus. Conclusion: NDRG 1 is probably related to the liver cell differentiation and is highly expressed in the specific stage of the differentiation. However, it could not be recognized as a marker of differentiation. The high expression of NDRG 1 in HCC indicates that HCC is not just distributed to a simple de differentiation. Much more study is necessary in understanding the comprehensive relationship of the disorder proliferation and differentiation of HCC and the related genes.