Objective To study the immune state and correlated strategy of patients with hepatic cirrhosis and primary hepatic carcinoma.Methods 17 patients with hepatic cirrhosis and 8 patients with primary hepatic carcinoma(PHC) in our hospital were collected since 2002.The indexcs of immune function were detected in all patients and some were re-examined.Results It was found that although the immune index varied in patients suffering from severe liver diseases,some regularities were shown as follows:(1)CD4 and CD4/CD8 in T lymphocyte subset declined markedly(P

Though prokaryotic cells could hardly express recombinant human beta nerve growth factor (rhNGF-?) with a proper three-dimensional conformation, using of E. coli as a host for industrial production of rhNGF-? is controversial. Recombinant human beta NGF was expressed in E. coli and was refolded in vitro. The isolated products was shown to be consistent with those expressed and secreted by CHO cells in biochemical characters by SDS-PAGE, RP-HPLC, mass spectrometry, N terminal analysis and bioassay determined using DRG and PC12 cells. The products can be acquired with 95% purity, 1.8 ng/U biological activity from both expression system,which remain invariable biological activity when lyophilized in excipient and store at 37℃?2℃, RH 75%?5% for 3 months. Moreover, the product refolded from inclusion bodies of E. coli shows the predominance in homogeneity and the lower cost.

Objective: To summarize our experience on perioperative anesthesia management of patients with crush syndrome caused by earthquake under field condition. Methods: The clinical anesthesia data of 10 patients with crush syndrome caused by China Wenchuan earthquake, who were treated under field condition, were retrospectively analyzed, and our experience on perioperative anesthesia management was summarized. Results: After volume therapy, urine alkalization, and stabilization of internal environment, the 10 patients underwent debridement, decompression by fasciotomy or amputation under epidural anesthesia or brachial plexus block plus psychological therapy and moderate tranquillizer. During the operation, the patients showed smooth respiratory and cardiovascular characteristics without pain. Conclusion: Under field condition, regional anesthesia can provide adequate analgesic effect during and after the surgery. Early fluid resuscitation and life support are vital to the safety and life of patients.

Objective To explore the influence of breast cancer treated with chemo-therapy and Shengxue decoction.Methods 91 Cases of breast cancer were randomly recurited into two groups:The western medicine treatment group (40 patients) was given anastrozole tablets and chemo-therapy; The Chinese and western medicine treatment group (51 patients) was given chemo-therapy and Shengxue decoction.Results The total effective rate of was 82％ and 53％ in the Chinese and western medicine treatment group and western medicine treatment group respectively,the difference was statistically significant (x2=19.74,P＜0.05).The peripheral blood's CEA and MMP-2 mRNA expression of western medicine treatment were [ (7.50 ±4.32)ng/ml、(116.32±67.40)ng/ml respectively],higher than the Chinese and western medicine treatment group [ (7.20 ± 3.22) ng/ml、(105.08 ± 37.82) ng/ml,respectively,P＜ 0.05 ],but the TIMP-2/MMP-2 (26.65 ±19.75) was significantly lower compared with the Chinese and western medicine treatment group [ (39.48±21.21) (P＜0.05) ].Conclusion The combination of traditional Chinese and western medicine was effective in treatment of breast cancer and well worth for popularization.

Objective To discuss the application value of multi- position radiography in the diagnosis for fracture of rib.Methods 98 cases with integrated data of thoracic trauma undergone multi- position view X-ray from 2007 to 2009 were retrospectively reviewed.Results The percent of fractures with orthophoria view is 65.4%.The percents of fractures with oblique position,tangential projection and spot film under the X-ray fluoroscopy are 20.6%、5.1% 、8.8%,while doesn' t find the A-P position,The total percent is 34.6%.Conclusion Multi-position radiography can elevate the diagnosis for fracture of rib and has application value.

Objective:To observe the efficacy of Chuankezhi injection combined with budesonide and formoterol fumarate powder for inhalation in the treatment of chronic obstructive pulmonary disease ( COPD) . Methods:Totally 120 patients with COPD were ran-domly divided into two groups:the observation group inhaled budesonide and formoterol fumarate powder for inhalation combined with intramuscular injection of Chuankezhi injection, and the control group inhaled budesonide and formoterol fumarate powder for inhala-tion. The treatment course was 28 days. The pulmonary function indices [ FEV1% expected value, FEV1 /FVC, 6 min walking dis-tance (6MWD)], the times of acute exacerbation and the adverse drug reactions during the treatment were observed and compared be-tween the two groups. Results:After the treatment, the lung function and 6MWD were improved in the two groups, and the improve-ment of FEV1% expected value in the treatment group was more obvious than that in the control group [(59. 7+12. 1)% vs (49. 8+11. 3)%, P<0. 05]. The number of patients with at least once of acute exacerbation was significantly reduced in the treatment group when compared with that in the control group (35% vs 62%, P<0. 01). The incidence of adverse drug reactions in the two groups showed no significant difference. Conclusion:Chuankezhi injection combined with budesonide and formoterol fumarate powder for in-halation can notably improve the lung function in the patients with COPD and reduce the times of acute exacerbation without effect on the incidence of adverse drug reactions.

The development and progress of gastric mucosal diseases is a complex process associated with multiple factors and underlying mechanisms,and individuals may present different genetic susceptibilities to gastric mucosal diseases. Various studies have demonstrated a strong link between Toll-like receptors( TLRs),as pattern recognition receptors,and the development and progress of gastric mucosal diseases. The gene polymorphisms of TLRs may change its encoded proteins or related signaling pathways,which results in different susceptibilities to gastric mucosal diseases;this might affect the development,progress and clinical outcome of the disease. In this review article,the advances in study on TLRs and its gene polymorphisms in gastric mucosal diseases were summarized.

Firstly, this paper analyzed the significance of strengthening medical students’ ecological moral ed-ucation under the view of Beautiful China. Secondly, it discussed the present situation of ecological moral and the reasons of lacking of ecological moral in medical students. At last, the authors pointed out the countermeasures to strengthen the moral cultivation of medical students’ ecological countermeasures under the view of Beautiful China. Therefore, we must strengthen the top-level design to ensure the ecological moral education enter into the national education system, enhance to maintain power to build the full-area coverage ecological moral teaching system, and strengthen institutional construction to establish long-term mechanism of ecologic moral education.

Alzheimer's disease (AD) is the most common form of dementia, which affects more than 35 million people worldwide with increasing tendency. AD brings stupendous economic burden for both the society and family. It has become a major global public health issue. Electro-acupuncture (EA) therapy has been widely used in AD treatment with satisfactory results. Based on the basic research, this paper explored the therapeutic effect of EA in AD treatment from the learning ability and morphological changes in AD animal models. From the cholinergic neuronal apoptosis theory, inflammatory response theory, oxidative stress theory and other aspects, the action mechanism of EA in the treatment of AD animal models were reviewed. At last, limitations of EA in AD treatment were analyzed. It proposed the outlook of a new type of EA-music EA in AD treatment. It provided a new idea and method for AD clinical treatment.

Objective: To inquire into the discrepancy in the behavior and therapeutic ways for the children at different age with dental diseases.Methods: 994 children at the age of 1～12 with dental diseases were divided into 3 groups on the base of their ages. Relevant therapeutic ways were taken according to their behavior. The influencing factors that made dental treatment finished smoothly were reached through analyzing the relationship among the children, the parents and the medical personals. Results: The manifestations of children's behavior had significant age differences when they received dental treatment. Non cooperative children constituted the majority of the infantile children, while the school age children were mostly cooperative. The ways chosen to treat children's dental diseases also had significant age differences. Compulsory means was the main way to treat infantile children and routine means to the pre school age and school age children. Inductive and threatening ways were always used for the non cooperative pre school age children. The non cooperative school age children were always received inductive means and seldom threatening and compulsory ones. The parents often accompanied with the infantile children, but did not with the pre school age and school age children. Conclusion: The age is a key factor to determine the clinical manifestations and the methods used to treat children's dental diseases.