Objective To explore the factors associated with infections in children with leukemia after chem-otherapy. Methods Clinical data of fifty-six children with leukemia, who infected after chemotherapy were reviewed with infection characteristics and some related factors. Results The infection rates were 27% in upper airway,37% in lower upper airway,12% in oral cavity,5% in gastrointestinal tract,3% in perianal abscess,7% in urinary sys-tem,5% in scpficaemia and 2% in ofitis media. Most infection happened from five to ten days after chemotherapy,and the infection rates varied with chemotherapy scheme. The persistence time of infection were (8.5±2.2) days in the children with the numbers of neutrophil below 0.5×109/L and (3.5±1.4) days in those above 0.5×109/L with significant difference(P <0.01). Conclusions After chemotherapy,the children had more chances to be infected. To be more exact, the /nfection was more common observed in respiratory tract, next in gastrointestinal tract. And the stronger the medicine used the number of heterophil granulocyte dropped much more ,the infection happened more easily.

Objective To explore the effect of transforming growth factor ?_1 (TGF-?_1) on expression of paxillin(Pax) in human kidney proximal tubular epithelial cell line (HKC cells) in different phases. Methods HKC cells cultured in vitro were divided into four groups at random. Control group(C group): HKC cells were cultured with serum free medium (FSM), and TGF-?_1-treated groups (T1, T2, T3 group): HKC cells were cultured with FSM containing 10ng/ml TGF-?_1. In the latter groups, duration of treatment varied as follows: T1 group for 24h, T2 group for 48h, T3 group for 72h. The proliferation of HKC cells induced by TGF-?_1 was assessed by MTT and the expression of Pax was determined. The gene expression of Pax was determined by reverse transcripton polymerase chain reaction (RT-PCR) and the protein expression of Pax was assessed by immunohistochemical staining and Western blot. Results TGF-?_1 could induce the proliferation of HKC cells in a time-dependent manner. In C group there was expression of Pax mRNA and protein in HKC cells. The expression of Pax mRNA and protein in HKC cells was positive in all the T groups, especially in T2 group and T3 group they were significantly increased compared with T1 group (P

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random: control group(C): cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2): cultured with FSM containing different concentrations of TGF?1 (T1: 5 ng/ml, T2: 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P

Objective To study the efficacy as well as side effects of the Nimotop in the treatment of benign memory disorders with oral tab. Method 38 patients with benign memory disorders as treatment group, taking Nimotop oral tab 30mg, Tid for 6 weeks and anathr 34patients with benign memory disorders taking Pyrrolidone Acetamide oral tab 0. 8, Tid as control group. Result The total efficiency were 73.6% in treatment group, while 47% in control group. Although some improvement in the memory scale and memory quotient were found in both groups, but the treatment group is obviously superior to control group ( P ＜ 0. 05) . Conclusion Nimotop has positive effect on the treatment of benign memory disorders.

Objective:To investigate the clinical effect of Pijiling Liniment for seborrheic dermatosis on 327 cases. Methods:327 patients with seborrheic dermatosis were treated with Pijiling Liniment. The clinical effects were compared with that of the control group(Co.Resorcinol Ointment, 76 cases). Results:The total effective rate and curative rate of the treatment group for seborrheic dermatitis were 93.5% and 33.3%, respectively. The effective rate for acne was 80.6% and for dermatomycosis microsporina was 7.5%. The total effective rate and curative rate of the control group were 92.1% and 2.6 %, respectively. Conclusions:There is a significant difference in the curative rate between two groups( P 0.05) through x 2 test.

The involvement of oxidization of peroxid in the antigen-antibody reaction makes the cell membrane permeability enhanced, so the fluorescence-labeled antibody stain can infiltrate into the cell easily and contact the antigen rapidly and diffusely, and then the antigen-antibody crosslinking can be formed efficiently. With the enhanced staining efficiency, shortened test duration, simplified operation, increased positive detection rate and accuracy, the new technology lays a foundation of clinical definite, curative effect view and prognosis.

OBJECTIVE To investigate the characteristics of mycoplasma infection and antifungal resistance to guide the clinical reasonable usage.METHODS Mycoplasma was isolated and its antibiotic susceptibility was detected by Mycoplasma IES test kit.RESULTS Among the 1103 samples,418(37.8%) were positive,including 268 with Ureaplasma urealyticum(Uu)(64.1%),13 with Mycoplasma hominis(Mh)(3.1%),and 137 with mixed infection(Uu and Mh)(32.8%),Young people with the age from 20 to 45 were the highest group of infection(80.1%),Sensitive antibiotics to mycoplasma in precedence order were doxycyline,minocycline,tetracycline,josamycin,and clarythromycin.CONCLUSIONS Mycoplasma is a common pathogen resulting in urogenital tract infection.We should pay more attention to monitor mycoplasma infection and use antibiotics properly and to guide clinically antibiotics usage.

Objective To explore the effect of heroin dependence on the expression and the function of glucagon in islet A cells of rats.Methods The changes of numerical density on area of A cells and level of plasma glucagon of heroin-dependent rats were investigated by means of the immunohistochemical SABC method,image analysis and radioimmunoassay(RIA).Results As compared with SCG and NCG,it was observed that the number of Glu-IR positive cells increased and the intensity of immunohistochemical staining enhanced in the heroin-dependent groups.The result of image analysis showed that the numerical density on area of A cell apparently increased in the 38-day heroin-dependent group(P

Objective To investigate the effect of Guizhi Fuling pills on levels of serum MAPK, ERK and VEGF in patients with endometriosis. Methods 60 patients with endometriosis were selected and according to the random control table were divided into the experiment group and the control group, 30 cases each.The control group were treated with conventional acetate goserelin sustained-release implantation agent subcutaneous injection, the experiment group were treated by Guizhi Fuling pill treatment.After treatment, the clinical efficacy, serum MAPK, ERK-5, VEGF, ERK, T-cad and VE-cad levels of the patients were compared.Results Compared with the control group, the clinical total effective rate was higher in the experiment group (P<0.05);MAPK and ERK-5 levels in serum were lower (P<0.05), ERK level in serum was lower(P<0.05);T-cad and VE-cad levels in serum were lower(P<0.05);VEGF level in serum was lower(P<0.05).Conclusion Guizhi Fuling pill can block the cell signal transduction pathway through inhibition of MEK and ERK activity, thereby inhibit abnormal endometrial proliferation and cell division cycle, can effectively inhibit the proliferation and differentiation of the tumor.