Objective: To study the diterpenes in the roots of Rabdosia japonica var. glaucocalyx. Methods: The constituents of R. japonica var. glaucocalyx were separated and purified with chromatographic methods, and their structures were elucidated by spectroscopic methods. Results: Two compounds were isolated from the roots of R. japonica var. glaucocalyx, and they were identified as glaucocalyxoside (1) and inflexuside A (2). Conclusion: Compound 1 is a new abietane diterpene glycoside named glaucocalyxoside, and compound 2 is isolated from R. japonica var. glaucocalyx for the first time.

Cardiovascular Diseases ; etiology ; prevention & control ; Humans ; Metabolic Syndrome

Cardiovascular Diseases ; prevention & control ; China ; Humans

Objective To investigate the effects of losing ratio of orthodontic brackets bonding after prolonged etching time (prolonged etching time method) and microabrasion with normal acid etching time (etching after microabrasion method) to different degree of dental fluorosis.Methods A total of 30 patients with dental fluorosis were selected from the cases treated in the Department of Stomatology,Lianyungang Hospital of Traditional Chinese Medicine.They were classified as 3 groups from mild to severe degree according to Dean's criteria,10 people per group.Patients within each group were randomly assigned to 2 subgroups,5 people per subgroup;prolonged etching time method and etching after microabrasion method were used,respectively,in each subgroup.The total and first losing ratios of brackets at five time points (1,2,4,12 and 24 weeks) were observed.The losing ratio differences of orthodontic brackets between the two disposing measures bonding to different degree of dental fluorosis were analyzed.Resuts The total bracket loss rates were 38.3％ (46/120) and 21.4％ (25/117),respectively,in severe dental fluorosis disposed by prolonged etching time method and etching after microabrasion method.It was significantly higher than that of the same processing method of mild and moderate dental fluorosis groups,in which the total bracket loss rates were 12.5％ (15/120),18.1％ (21/116),7.6％ (9/118) and 5.8％ (7/120),respectively,and the differences were statistically significant (x2 =21.12,11.87,8.96,12.24,all P ＜ 0.01).The total loss rate of etching after microabrasion method was lower than that of prolonged etching time method in mild dental fluorosis group,the difference was not statistically significant (x2 =1.56,P ＞ 0.05).The total loss rates of etching after microabrasion method were lower than that of prolonged etching time method in moderate and severe dental fluorosis groups,and the differences were statistically significant (x2 =8.49,8.13,all P ＜ 0.05).The medians of first bracket loss rates were 8.3％ and 4.3％,respectively,in severe dental fluorosis disposed by prolonged etching time method and etching after microabrasion method.They were significantly higher than that of the same processing method of mild and moderate dental fluorosis groups,in which the medians of first bracket loss rates were 2.5％,4.3％,1.7％ and 0.8％,respectively,and the differences were statistically significant (U =2.619,2.627,2.312,2.627,all P ＜ 0.05).The average percentage of first bracket loss rates of etching after microabrasion method was lower than that of prolonged etching time method in mild dental fluorosis group,the difference was not statistically significant (U =-0.949,P ＞ 0.05).The average percentages of first bracket loss rates of etching after microabrasion method were lower than those of prolonged etching time method in moderate and severe dental fluorosis groups,and the differences were statistically significant (U =-2.214,-2.410,all P＜ 0.05).Conclusions Whether in prolonged etching time method subgroup or etching after microabrasion method subgroup,the more serious injury of dental fluorosis is,the higher loss rate of orthodontic bracket will be.For the clinical treatment of mild and severe dental fluorosis,the effect of etching after microabrasion method is better than prolonging etching time method.

OBJECTIVE:To study tanshinone ⅡA inhibiting the proliferation and migration induced by homocysteine(Hcy)of rat aortic vascular smooth muscle cells(VSMCs)and its signal pathway. METHODS:VSMCs were selected for the following ex-periments. In order to validate TanshinoneⅡA inhibiting the proliferation and migration induced by Hcy of VSMCs,VSMCs were di-vided into control group,Hcy group(1 000μmol/L),TanshinoneⅡA low-dose,medium-dose and high-dose groups(5,10,20 μg/L). In mechanism study test,VSMCs were divided into control group,TanshinoneⅡA group(20 μg/L),rapamycin group(20 nmol/L), MHY1485 group(10μmol/L),TanshinoneⅡA+rapamycin group(TanshinoneⅡA,20 μg/L+rapamycin,20 nmol/L),TanshinoneⅡA+MHY1485 group (TanshinoneⅡA,20 μg/L+MHY1485,10 μmol/L). In validation test of P70S6K and p-P70S6K pathway expres-sion,rapamycin and MHY1485 were used for inhibitory test and activation test,respectively. The proliferation of VSMCs was de-termined by ELIASA,and Transwell chambers and wound healing test were employed to test the migratory ability of VSMCs. West-ern blotting were used to investigate the expressions of P21,P27,MMP-2,MMP-9,P70S6K and p-P70S6K in VSMCs. RE-SULTS:In validation test,compared with control group,24,48 h absorbance,migration area,the number of VSMCs penetration and the expression of MMP-2,MMP-9 and p-P70S6K increased significantly,while the expression of P21 and P27 decreased sig-nificantly(P<0.01). Compared with Hcy group,48 h absorbance of TanshinoneⅡA low-dose group,24,48 h absorbance and the expression of MMP-2 of TanshinoneⅡA medium-dose and high-dose groups,migration area,the number of VSMCs penetration, the expression of MMP-9 and p-P70S6K in low-dose,medium-dose and high-dose groups all decreased significantly;the expres-sion of P21 and P27 increased significantly in TanshinoneⅡA medium-dose and high-dose groups(P<0.01);there was no statisti-rapamycin group,while 24,48 h absorbance increased significantly in MHY1485 group(P<0.01). Compared with TanshinoneⅡA group,24,48 h absorbance decreased significantly in TanshinoneⅡA+rapamycin group,while 12,24,48 h absorbance,migration area and the number of VSMCs penetration increased significantly in TanshinoneⅡA+MHY1485 group (P<0.01). In inhibitory test,compared with control group,the expression of p-P70S6K decreased significantly in TanshinoneⅡA group (P<0.01);com-pared with TanshinoneⅡA group,the expression of p-P70S6K decreased significantly in rapamycin group and TanshinoneⅡA+rapa-mycin group(P<0.01);in activation test,the expression of p-P70S6K decreased significantly in TanshinoneⅡA group(P<0.01), compared with TanshinoneⅡA group,the expression of p-P70S6K increased significantly in MHY1485,TanshinoneⅡA+MHY1485 group (P<0.01). CONCLUSIONS:TanshinoneⅡA can inhibit the proliferation and migration of VSMCs by suppressing mTOR/P70S6K signal pathway.

Hematopoietic stem cell transplantation (HSCT) has been widely performed for patients with various genetic and acquired malignant and non-malignant diseases.Central nervous system (CNS) complications are considered an important cause of morbidity and mortality after HSCT.This review discuss the main causes of CNS after HSCT such as infection,drug toxicity,graft-versus-host disease,metabolic encephalopathy,neurologic relapses etc,so as to benefit the early recognition of CNS complications and to improve the prognosis of patients after HSCT.

Objective To observe the efficacy of the combination of moxibustion and pressing beans on auricular in the treatment of elderly patients with chronic diarrhea.Methods 45 elderly patients met the diagnostic criteria for diarrhea were divided into two groups,the treatment group (23 cases) received the treatment of combined moxibustion with ear pressure beans,the control group (22 cases) received traditional Chinese medicine treatment.The efficacy was observed and compared after two weeks.Results The effective rate reached 95.6％ in the treatment group,81.8％ in the control group (statistically significant difference,odds ratio 1.16).Conclusion Combined ear pressure beans with moxibustion treatment will receive a significant effect for the elderly patients with chronic diarrhea.The therapy is worth clinical application.

Objective To observethe effect of continuous veno-venous hemofiltration (CVVH) on serum level of antibrain-antibody (ABAb) in patients with severe traumatic brain injury.Methods Thirtysix patients with severe traumatic brain injury were randomly divided into control group (19 patients) and CVVH group (17 patients).The control group accepted conventional treatment,the CVVH group accepted CVVH treatment additionally for 7 d.ELISA was used to measure serum level of ABAb at 1,3,5,7,14 d after treatment.The change of ABAb in serum of each group was observed.The scores of Glasgow coma scale (GCS) was assessed everyday.Results The level of ABAb in CVVH group was significantly lower than that in control group from the third day after treatment[the third day:(1.28 ± 0.25) kU/L vs.(1.33 ± 0.30) kU/L;the fifth day:(1.37 ±0.17) kU/L vs.(1.42 ±0.22) kU/L;the seventh day:(1.45 ±0.37) kU/L vs.(1.52 ± 0.35) kU/L;the fourteenth day:(1.49 ± 0.41) kU/L vs.(1.67 ± 0.39) kU/L,P ＜ 0.05 or ＜ 0.01].The scores of GCS in CVVH group was significantly higher than that in control group from the seventh day after treatment [the seventh day:(6.95 ± 1.24) scores vs.(5.74 ± 1.51) scores;the fourteenth day:(9.29 ± 1.76) scores vs.(7.22 ± 1.24) scores] (P ＜ 0.01).Conclusion CVVH can decrease the level of ABAb and improve the prognosis.

Objective To explore the value of electroencephalogram (EEG) in children with headache.Methods 180 children with headache were carried out EEG recording,and the electrical activity of the brain was recorded and analyzed.Results In 180 cases of children,EEG was normal in 56 cases,abnormalities in 124 cases,the abnormal rate was 68.89％.The mild abnormalities was 78 cases,in a proportion to 43.33％ in the total record:moderate abnormalities was 14 cases,in a proportion to 7.78％ in the total reccrd;high-grede,abnormalities was 2 cases,in a proportion to 1.11％ ;5 patients was in critical state,in a proportion to 2.78％ ;epileptiform activity was 25 cases,in a proportion to 13.89％.97 normal cases with intermittent headache,abnormal rate was 46.1 1％,which was significantly lower than the attack headache ( x2 =15.16,P ＜ 0.05 ).Conclusion Headache in children with EEG examination had important significance.

Objective To observe the change of serum anti-brain antibodies after cardiopulmonary resuscitation,and investigate the clinical significance.Methods A total of 27 cases of cardiopulmonary resuscitation after cardiac arrest patients with success and survival more than 12 weeks were divided into two groups according to Glasgow Coma Scale (GCS) score:mild and moderate group with GCS score ≥ 8 scores (12 cases) and severe group with GCS score≤7 scores (15 cases).The serum anti-brain antibody levels at 3,7 days and 2,3,4,12 weeks after recovered in the spontaneous circulation (ROSC) was compared between two groups,and compared with control group (15 cases of healthy persons).Results The serum anti-brain antibody levels at 3,7 days and 2,3,4,12 weeks after ROSC were significantly higher than those in control group [(1.34 ± 0.23),(1.30 ± 0.27) kU/L vs.(0.28 ± 0.05) kU/L,(1.38 ± 0.33),(1.44 ± 0.30) kU/L vs.(0.28 ±0.05) kU/L,(1.44 ±0.31),(1.51 ±0.33) kU/L vs.(0.28 ±0.05) kU/L,(1.53 ±0.27),(1.67 ±0.36) kU/L vs.(0.28 ±0.05) kU/L,(1.72 ±0.25),(1.93 ±0.44) kU/L vs.(0.28 ±0.05) kU/L,(1.98 ±0.45),(2.15 ±0.52) kU/L vs.(0.28 ±0.05) kU/L],and there were significant differences (P＜ 0.01).The serum anti-brain antibody levels in severe group were significantly higher than those in mild and moderate group,and there were significnat differences (P ＜ 0.05).Conclusions The serum anti-brain antibody levels after cardiopulmonary resuscitation rise significantly.Anti-brain antibody may be used as a biochemistry marker to judge degree and prognosis of brain injury with patients after cardiopulmonary resuscitation.