Objective To investigate the analgesic effect of warm needling and explore its relationship with the function of mast cells in the acupoint area and the specificity of acupoints.Method Sixty SD rats were randomized into blank (C), model (M), warm needling Zusanli (Z), contralateral warm needling (O), manual acupuncture (A), warm needling Futu (F), warm needling Yanglingquan(Y), warm needling Weizhong (W), disodium cromoglycate + warm needling (DSCG+Z) and saline + warm needling (Saline+Z) groups. A rat with adjuvant arthritis was used as a model of inflammatory pain (AA model). Hindpaw withdrawal latency was measured in therats during warm needling at Zusanli (ST36), Yanglingquan (SP9), Futu (ST32) and Weizhong (BL40). Pre-treatment and post-treatment rates of mast cell degranulation in the acupoint area were compared and the effect of acupoint area injection of disodium cromoglycate on the degranulation was observed by acupoint tissue section staining.Result Obvious degranulation of mast cells in the acupoint area appeared after warm needling (P>0.05). The analgesic effect of warm needling was better than that of manualacupuncture (P>0.05). After treatment, pain threshold was significantly higher in the warm needling Zusanli group than in the warm needling Futu, warm needling Yanglingquan and warm needling Weizhong groups (P>0.05). Acupuncture-induced mast cell degranulation could be prevented by disodium cromoglycate (P>0.05).Conclusion Warm needling at point Zusanli can produce an analgesic effect in adjuvant arthritis rats. Its analgesic effect is better than that of manual acupuncture. The rate of mast cell degranulation in the acupoint area is higher in the warm needling Zusanli group than in the manual acupuncture group. The analgesic effect of warm needling is reduced after pretreatment with disodium cromoglycate, a mast cell stabilizer. In an AA model, the analgesic effect of warm needling at point Zusanli is better than those of warm needling at points Yanglingquan, Futu and Weizhong.

Objective:Our purpose was to establish an ideal chronic pressure-afterload heart failure rat model which has the transition from cardiac hypertrophy to heart failure. Methods: Chronic pressure-afterload heart failure rat model was induced by gradually constricting the ascending aorta of young rats. Young rats were randomly divided into 2 groups: the constricted and sham-operated groups. Clinical manifestation, tail-cuff blood pressure, organ weight, and hemodynamic data were observed at various time after operation. Results: The overall survival rate was 87%. Tail-cuff pressure began to increase in 4 weeks after operation. Left ventricular hypertrophy appeared in 12 weeks and heart failure in 5 months. Conclusion:It's a practical and reproducible model of cardiac hypertrophy that progresses to chronic heart failure.

Objective Applying a reliable precise method to assess the mitotic index of cardiomyocytes,to disclose of disclosure the mechanism implicated in cardiomyocytes proliferation. Methods H9c2(2-1) cardiomyocytes were originally developed from rat BD1X heart(ATCC).These cells were cultured on coverslips.Double immunofluorescence staining with monoclonal antibodies(1:100) against phospho-histone H3 and ?-sarcomeric actin was performed on the cultured cells.Anti-mouse IgG FITC was used as the secondary antibody for the H3P antibody,and anti-mouse IgM Cy3 was used as the secondary antibody for the ?-sarcomeric actin.DNA was visualized with Hochest 33342.All photographs were taken with an Olympus fluorescence microscope. Results The cytoplasm of cardiomyocytes appeared red,the mitotic chromosomes green with distinct shape,and Hochest 33342-stained nuclei blue.Conclusion Our method is the reliable and exact means to observe and assess cardiomyocytes mitosis.

We report a rare case of pleomorphic adenoma arising from the nasal septum. A 37-year-old woman presented with a 1-year-history of right-sided occasional epistaxis. Computed tomographic scans revealed an oval mass in the right nasal cavity. The tumor was removed endoscopically with endonasal approach. The microscopic finding showed numbers of myoepithelial cells and duct-like structures consisting of loose myxoid stroma. This lesion had histological characteristics of a pleomorphic adenoma, and this was confirmed by immunohistochemical expression of cytokeratin, S-100 protein and SMA. Her post-operative course was uneventful, and she is currently free from the disease 1.5 years after surgery.
Actins ; metabolism ; Adenoma, Pleomorphic ; diagnosis ; surgery ; Adult ; Endoscopy ; Epistaxis ; Epithelial Cells ; Female ; Humans ; Keratins ; metabolism ; Nasal Septum ; pathology ; Nose Neoplasms ; diagnosis ; surgery ; S100 Proteins ; metabolism

Objective To explore the nursing measures of teenagers with arthroscopic reduction of avulsed fracture of tibialintercondylar eminence. Methods Eighteen teenagers with avulsed fracture of tibialintercondylar eminence hospitalized from January 2013 to September 2014 were included in the study. Before operation, psychological care and operative preparation were delicately done. After operation, a plan for function training was made, the preventive measures for related complications were taken and discharge instruction was performed. Results All patients got recovered after function training 3 months since operation. The average score by Lysholm evaluation was (97.6 ± 2.3). Among them, one was Lachman test I positive, with mildly limited extension of the knee join. Conclusion The following nursing strategies inclucling psychological nursing before operation can help to reduce the incidence of complications.

Objective To establish a HPLC method for the content determination of Chlorogenic acid and Hyperoside in the leaves of acanthopanax senticosus from different areas.Methods The samples were separated on Hedera? ODS-2 C18 column,using acetonitrile-0.4% phosphoric acid(17∶83) as mobile phase,the detection wavelength was 360 nm.Result Chlorogenic acid and Hyperoside showed good linear relationship over the ranges of 10.2～306 ?g/mL(r =0.999 5) and 10.4～312 ?g/mL(r =0.999 8),respectively.The average recoveries were 97.14% and 98.21% with RSD at 1.65% and 1.09%,respectively.The contents of Chlorogenic acid and Hyperoside were different obviously in samples from different areas.Conclusion This method is simple,rapid and sensitive,and it can be used for the further development and the quality control of the leaves of acanthopanax senticosus.

Objective To assess the regional left ventricular (LV) rotation motion in the patients with rheumatic mitral stenosis (MS) with velocity vector imaging (VVI).Methods This study included 38 patients with isolated MS (mild,moderate and severe) and 55 healthy control subjects.Short-axis parasternal views at the basal and apical level of LV were taken.Peak value of rotation angle and rotation velocity,as well as time to peak value were measured by VVI workplace offline respectively.Results ①In the healthy control subjects LV rotates clockwisely at the base and counterclockwisely at the apex,the same as the patients with MS.② In th patients with MS,rotaion angle and rotation velocity were significantly reduced at the apical level(P ＜0.001),rotation velocity were significantly reduced at some segments of basal level (anterior,lateral,septal) (P ＜ 0.05).③Compared with the healthy control,the difference of time to peak roation angle between base and apex was significantly longer in patients with MS (P ＜0.05).④ There were no significant differences among mild,moderate and severe MS (P ＞0.05).Conclusions The patients with MS may suffer from systolic dysfunctin even in the early stage identified by the alteration of LV rotation motion,which is independent of the hemodynamic severity of MS.

Objective To detect the serum anti‐sialic acid glycoprotein receptor antibody (anti‐ASGPR) levels in the patients with chronic hepatitis B(CHB) ,chronic hepatitis C (CHC)and healthy people ,and to observe the relationship between anti‐ASGPR and the disease development in the patients with hepatitis .Methods Totally 60 patients with hepatitis B virus (HBV) infection(30 cases of CHB and 30 cases of CHB cirrhosis) and 60 patients with hepatitis C virus (HCV) infection(30 cases of CHC and 30 cases of CHC cirrhosis) were selected with 60 persons undergoing the physical examination as the control group .The anti‐ASGPR and ALT levels in all research subjects were detected with ELISA .Results (1)The anti‐ASGPR level in the HBV and HCV infection groups was significantly higher compared with the control group ,and the difference was statistically significant(P<0 .01) .The an‐ti‐ASGPR level in the CHB cirrhosis group was significantly higher than that in the CHB group ,and the difference was statistically significant(P<0 .05) .The anti‐ASGPR level in the CHC cirrhosis group was significantly higher than that in the CHC group ,and the difference was statistically significant(P<0 .05) .No correlation between anti‐ASGPR and ALT was found .(2) The anti‐ASG‐PR level in the CHC group was significantly higher than that in the CHB group ,and the difference was statistically significant(P<0 .01) .Conclusion The detection of anti‐ASGPR is helpful for clinical differential diagnosis and has an important significance for the treatment and prognosis .

OBJECTIVE:To determine camphor in compound menthol nose drop by RP-HPLC.METHODS:Waters No-va-pak C 18 was taken as the chromatographic column,the mobile phase was composed of chloroform-methanol-water(14∶52∶30)with a flow speed at1.0ml/min and detection wavelength at289nm,the column temperature was35℃.RESULTS:The linear range for camphor was0.8～5.6?g/ml(r=0.9998)and the average recovery rate was99.4%(RSD=0.7%).CONCLUSION:The method is specific,convenient and accurate,which can be used for the quality control of the preparation.

Alzheimer's disease(AD),the most common form of dementia,it is lack of effective cure or preventive treatment.Dementias in the elder are an increasing medical,social and economic problems and current treatments are only mildly effective.Recently,amyloid-beta protein(A?) has become a major therapeutic target.A? vaccine treatment can improve cognition in the patients with AD,but adverse events,such as meningencephalitis were observed in clinical study.The passive A? immunotherapy in humans is effective with possible safety.However,patients need to be monitored carefully.