Objective:To understand the status quo of community care in Chongqing city in 2006. Methods:To know the basic state of community care by observation and data collection in 9 community health service centers and 18 service stations that located in urban area,we investigated 104 nurses and 62 patients in communities by questionnaire. Results:The average community nurses are older than hospital nurses;98% community nurses are below the university academic career;some service items such as home nursing,health care,rehabilitation nursing and hospitalpice are not totally completed;there are manifest differences in community service among nurses with different educational background and different professional titles(P﹤0.05). Conclusion:An alternate institution may be established between general hospitals and community health centers(station). The finance support to community medical and training of community nurse should be guaranteed and service range of community care should be expanded.

BACKGROUND: Glass ionomer cement has been a commonly used filling matedal in the clinic. However, glass ionomer easily absorbs water and dissolves in early concretionary phase, which can reduce the leak tightness of the edge of obturator, resulting in slightly leakage. Therefore, protective agents are necessary following glass monomer filling. OBJECTIVE: To compare the effects of three protective agents (vaseline, cocoa butter, luminous paint) on edge closeness of glass ionomer cements. DESIGN, TIME AND SETTING: The controlled observation study was performed at the Central Laboratory, Hospital Affiliated to Medical College, Qingdao University from July to October 2007. MATERIALS: Forty freshly block teeth were used. After cleaning the facing, a 5 mm×3 mm×2 mm cavity was made at 1/3 part near to buccal surface using a high speed turbine engine under water cooling. Tooth facing was coated twice with colorless nail-polish at1 mm from the cavity. Apical foramen was covered with rod wax sheet. Teeth were equally and randomly assigned into control group, vaseline group, cocoa butter group and luminous paint group. METHODS: Glass ionomer cement was filled into the cavity. The filling surfaces were respectively coated with vaseline, cocoa utter and luminous paint in each group. The control group was left intact. Hot-cold cycle test and microleakage test were performed. MAIN OUTCOME MEASURES: The depth from dye penetration into the cavity wall to filling was recorded. RESULTS: Microleakage to different degrees was observed in each group under the microscope. However, the penetration depth was deeper in the control group than other three groups (P ＜ 0.05). No significant difference in penetration depth was detected among the vaseline, cocoa butter and luminous paint groups (P ＞ 0.05). CONCLUSION: Vaseline, cocoa butter and luminous paint have good effects on wet prevention.

Objective To understand the comprehensive quality status of community nurses in Chongqing and seek the development strategy of community nursing education. Methods Related infor-marion was collected through questionnaire investigation in 104 community nurses from 9 community health service center and 18 community health station. Results The mastering degree of various community nursing knowledge was different among nurses with different education background and different profession-al titles (P ＜ 0.05).More than 80% nurses considered it necessary to cultivate comprehensive technique of special courses ,the ability to cope with community emergencies and self-development in their work.78.9% nurses thought they should reinforce the ability in predicting potential problems in community and solving problems by theirselves, Conclusions We need to strengthen the work belief of community nurses,perfect training conditions,optimize course contents and cultivate nurses' comprehensive ability purposely.

Objective To investigate the effect of electrical stimulation of cerebellar fastigial nucleus (FN) on the contents of monoaminergie transmitters in frontal lobes of rats with depression, and to explore the pathway and mechanism of electrical stimulation of cerebellum in treating depression. Methods A total of 30 Wistar rats were used tO establish the animal depression model by exposure to chronic stressing circumstances. Electrical stimulation delivered to the left cerebellar FN or left dentate nucleus (DN) for 1 h was used as the intervention. The contents of serotonin (5-HT), noradrenalin (NE) and dopamine (DA) in frontal lobe were determined with fluorospectropho-tometry. Results Compared with normal control group, the contents of 5-HT and NE in bilateral frontal lobes of depression model rats decreased significantly (P < 0. 001). No significant change of all three monoaminergic trans-mitters in normal rats was observed after stimulation of FN or DN (P > 0. 05), but significant increases of 5-HT and NE in right and left frontal lobes were observed in depression model rats after stimulation of FN (P < 0.05, P < 0.001; P <0.05, P <0.01), with the increase in right lobe more significant than in left lobes (P <0.05). In de-pression model rats underwent DN stimulation, significant increase of contents of all three monoaminergic transmitters in frontal lobes was observed. Conclusion ElectrieM stimulation of cerebellar FN can increase the contents of 5-HT and NE in frontal lobes of rats with depression, and this suggest that electrical stimulation might have therapeutic effect to depression.

As a T cell subsets growth factor,interleukin-2 (IL-2) can inhibit cancer cell proliferation,reduce tumorigenicity and transitivity,improve local tumor microenvironment,enhance the immunogenicity of hepatocellular carcinoma cells,reduce the load of hepatitis virus and greatly enhance the ability of anti-tumor.On the other hand,it can enhance tumor hypercoagulable state.The effect of IL-2 on primary hepatic carcinoma and its potential clinical value have a bright future.

Objective To observe the effect of human telomerase reverse transcriptase-thymidine kinase/ganciclovir (phTERT-TK/GCV) system combined human telomerase reverse transcriptase-tumstatin (phTERT-tumstatin) system on apoptosis of human HepG2 and mRNA expression and protein content of AFP,RhoC related with cancer.Methods Fluorescence microscopy was used to observe expression of EGFP and MCHERRY in transfected HepG2 and L-02.Real-time PCR and Western blot were used to detect AFP and RhoC mRNA and protein content.Flow cytometry was used to detect the apoptosis of transfected HepG2.Results phTERT-tumstatin and phTERT-TK/GCV genes expressed in transfected HepG2.Real-time PCR showed that AFP and RhoC mRNA expression in different group were 0.76±0.09 and 0.80±0.04 (TK/GCV group),0.62±0.09 and 0.40±0.02 (TM group),0.49±0.07 and 0.54±0.03 (MK group).The differences were significant (P ＜ 0.01) except TK/GCV group compared with empty plasmid group.Western blot test results showed that protein content of AFP and RhoC were higher in TK/GCV group (0.97±0.02/1.17± 0.01),TM group (0.83±0.02/0.99±0.02),MK group (0.69±0.01/0.77±0.02) than in empty plasmid group (1.19±0.03/1.32±0.05) and non-transfected group (1.15±0.05/1.29±0.30) (P ＜ 0.01).Additionally,protein content of AFP and RhoC in MK group were significant difference with TK/GCV group and TM group (P ＜ 0.01).Flow cytometry showed that phTERT-tumstatin,phTERT-TK/GCV co-transfected HepG2 cells apoptosis rate was significantly higher than both individually transected group.Cells apoptosis rate of alone and co-transfected groups was significant difference compared with empty vector group and untransfected group.Conclusions The effect of phTERT-TK/GCV and phTERT-tumstatin on pro-apoptotic of HepG2 cells is significant.phTERT-TK/GCV combined with phTERT-tumstatin has strong therapeutic function.

This paper introduces the development situation and mode of physician group in China, and analyzes its main legal risks and countermeasures.The main legal risks include: the nature of the Physician group is fuzzy and specific laws and regulations lack a clear stipulation;the legitimacy of the cooperative mode of the physician group is difficult to define and lacks legal protection for its contract effectiveness and implementation;the practice is driven by the interests and loopholes in legal norms and physicians may illegally transfer the patients;the multipoint practice policy is not perfect and brought legal risks for the development of the physician group.Therefore, the following measures are proposed to take adequate precautions: firstly, the nature of the physician group and its standards of supervision and taxes should be identified and clarified;secondly, the legitimacy standard for the physician group should be established for its cooperation mode to standardize the doctors` behavioral standards;and finally, we should make sure the multipoint practice policy and physician practice law are coherent during implementation.

Objective:To investigate the relation of counselors'and therapists'interpersonal personality on the ethical behavior of dual relationship.Methods:Totally 177 practitioners (21-65 years old) were investigated with 4 interpersonal personality subscales of the Chinese Personality Inventory (CPAI) and the Questionnaire of the attitude of counselors to the ethical behavior of dual relationship.Results:There were no differences of 4 dimension of interpersonal personality on the 6 items of professional relationship between higher and lower groups (Ps ＞ 0.05),but on the other 5 types of dual relations respectively,there were 6 (9％),10 (15％),12 (18％) and 21 items'(32％) score showing statistically difference (Ps ＜ 0.05).Furthermore,more items had score difference on social relationship.Conclusion:It suggests that the counselors'and therapists'interpersonal personality may be related to the ethical behavior in dual relationship.

Diabetic peripheral neuropathy( DPN) is one of the most common chronic complications and the disabling one, which is related to chronic hyperglycemia and microvascular changes. Currently, the treatments of diabetic peripheral neuropathy contains glu-cose control,pain management,etiological treatment,prevention and remove risk factors. So we make a review on the progress on the treatment of DPN by now , providing a scientific basis to a advanced and effective program.

0.05) and that of cure rate was significant ( P