In recent years,the efficacy of cervical cancer treatment has been increasing gradually.It has got a basic consensus of the standard treatment mode for most cervical cancer,but further effort should be done to improve the treatment response of locally advanced cervical cancer and recurrent or metastatic cervical cancer.The efect of redical surgery and radiotherpy is the same to early cervical cancer.Concurrent chemoradiotherapy is the standard treatment for patients with locally advanced cervical cancer.Chemotherapy combined with antiangiogenic targeted therapy can improve the survival of patients with recurrent or metastatic cervical cancer,while whether needing new adjuvant therapy or not,it asks for further investigation.

In order to select a computed tomography(CT)scanner system with reasonable cost performance (performance-price ratio) among various brands and types of CT products in accordance with the clinical features of the pediatric patients, Tianjin Children's Hospital made a feasibility study on purchasing the equipment in all its aspects, including the evaluation of its type and performance, social and economic benefits, and eventually purchased a 16-slice helical CT scanner which fully fulfills the practical needs of the hospital. This way of evaluation can be widely used in the feasibility study for purchasing different large-scale medical equipment to avoid unnecessary waste of funds.

Objective: To investigate the antitumor effect of SGI-1776 on human ovarian cancer HO-8910 cells and its molecular mechanism. Methods: HO-8910 cells were cultured in vitro, and the proliferation inhibitory effects of SGI-1776 were determined by MTT assay and colony formation assay. The effect of SGI-1776 on the distribution of cell cycle phase was observed by flow cytometry with propidium iodide (PI) staining. hTe inhibition rate of migration and invasion were valued by transwell cell assay. Multiple molecular techniques, such as ELISA, Western blot, siRNA and cDNA transfection were used to explore the molecular mechanism. Results: SGI-1776 presented dramatic anti-tumor activity against HO-8910 cells in vitro, inhibited the cells proliferation and colony formation, and attenuated the migration and invasion in a dose-dependent manner, accompanied by cell cycle arrest in G1 phase. SGI-1776 caused the proliferation inhibition with concomitant decrease in Pim-1 kinase activity, down-regulated the expression of Pim-1 protein and and its downstream genes, such as CDK6, pCDK6, CDK4, pCDK4, CDK2 and pCDK2, and increased the expression of P21 and P27. Down-regulation expression of Pim-1 by siRNA followed SGI-1776 treatment resulted in enhanced cell proliferation inhibition rate and attenuated migration/invasion. Up-regulation of Pim-1 by cDNA transfection attenuated SGI-1776-induced cell proliferation inhibition and its migration/invasion. Conclusion: Pim-1 mediates the biological effect of SGI-1776 in human ovarian cancer HO-8910 cells, suggesting Pim-1 might be a novel target for human ovarian cancer.

Objective To investigate the clinical characteristics of Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡ.Methods The clinical data of 1 case Kennedy disease with secondary mitochondrial dysfunction and glutaric aciduria typeⅡwas retrospectively analyzed.Results The patient presented muscle weakness in proximal limbs, fasciculation, bulbar palsy, amyotrophy, postural tremor, sensory disturbance, gynaecomastia, impotency.The level of serum creatine kinase and urinary glutaric acid were elevated. Electromyogram and nerve and muscle biopsy supported mixed damage in peripheral nerves, as well as neurogenic and secondary myogenic pathologic changes in muscles.Gene sequencing indicated trinucleotide CAG repeated amplification for 47 times in chromosome X.Diabetes was diagnosed at 7 years after onset.Conclusions The pathogenesis of Kennedy disease is very complex, which may involve myogenic factor.The treatment to the secondary mitochondrial and lipid metabolic disturbance in muscle can improve the muscle weakness to a certain extent.

ObjectiveTo investigate the change of the platelet function in children with primary immune thrombocy-topenia (ITP) before and after treatment.MethodsThe platelet indices, immature platelet fraction (IPF%), immature platelet counts (IPC), granule membrane glycoproteins of platelet (CD62p, PAC-1, CD42b) were tested by automatic blood cell analyzer and lfow cytometry (FCM) in 18 children with ITP before treatment and after complete response and in 17 children undergoing elective surgery (control gourp).ResultsCompared with control group, MPV, PDW, P-LCR and IPF% were signiifcantly higher and PLT, PCT, IPC were signiifcantly lower in children with ITP. The expressions of three platelet membrane glycoproteins (PAC-1, CD62p, CD42b) were signiifcantly lower in children with ITP. After treatment, children with ITP achieved completely response. Compared with before treatment, MPV, PDW, P-LCR and IPF% were signiifcantly lower, and PLT, PCT, IPC, and the expressions of three platelet membrane glycoproteins (PAC-1, CD62p and CD42b) were significantly higher. ConclusionsThe platelet function is depressed and in status of low activity in children with ITP. The relative platelet indices provide new references for the diagnosis and the judgment of therapeutic effect in ITP patients.

Since Chinese Regulation of Human Organ Transplantation being enforced in practice on May 1,2007,many related issues have arisen and drown our attention.One typical example is the mutual kidney transplantation issue between two families in Hunan province.Based on analyzing the pros and cons in this real event of disputes,the paper discusses ethical issues related to mutual kidney transplantation as follows.Whether or not this exchange approach of organ transplantation can be justified in order to save human lives? Whether or not the mutual transplantation style should be expanded to multiple families? How to handle the gap of organ supply shortage during practical transplantation between two or among several families? How to coordinate the disputes between mutual kidney transplantation and the now in-force legal regulations on organ transplantation?

Shenghua decoction made by FU Qing-zhu is a famous formula aimed at syndrome of coagulation cold due to blood deficiency,static blood obstruction.The mechanism of disease is definite when this formula is applied.But the extensive use of Shenghua decoction,especially some potent preparation of it following childbirth without according to TCM principle based on differentiation of syndromes is a manifestation of highlighting formula and underestimating theory,which is useless and harmful.It should be emphasized.So the Shenghua decoction should be used under the direction of basic theory of TCM,through precise syndrome differentiation,according to the principle of "looking to and not being besieged by postpartum ".We should not follow blindly the method of treatment of "warming and resolving stasis" on the postpartum disease.It is important that Shenghua decoction should be used on the basis of syndrome differentiation.

BACKGROUND: There is a close correlation between telomere length and cell senescence, immortalization or canceration. OBJECTIVE: To explore the significance of telomere length diversifies in the chronic compartment syndrome in lumbosacral region. DESIGN, TIME AND SETTING: A comparative observation was performed at the State-Level Key Medical Genomics Laboratory, Shanghai Jiao Tong University School of Medicine from July 2007 to February 2008. PARTICIPANTS: The vertical spinal muscular tissue samples were taken from people aged 29-49 years old [mean aged (34.7?12.6) years] including 10 normal people and 15 patients with chronic compartment syndrome. According to the age, the normal were divided into the aged 30-37, and aged 38-45 groups. The abnormal were divided into aged 29-34, aged 35-43 and aged 44-49 groups, with 5 samples in each group. METHODS: DNA was extracted from samples of each group. Telomere length in vertical organizations spinal muscular skeletal muscle was measured by Southen Blotting hybrid technology. MAIN OUTCOME MEASURES: Telomerase length of erector spinal muscles in patients and normal people. RESULTS: The mean telomere length in patients with chronic compartment syndrome was obvious shorter than that of the normal people, which has significant difference (P

Objective To design and establish a set of diffusion optical tomography(DOT) system.Methods Near-infrared(NIR) laser was employed as the light source and only one photomultiplier tube(PMT) as the detector.An optical multiplexer was used to alter the detector channels.The 32 channels of the system,which are consisted of 16 launch channels and 16 detector channels,worked under the continuous-wave(CW) model and were used to acquire 256 boundary data.Results Experiments were performed based on the proposed imaging system.The intralipid was used as the tissue-like medium and the India ink as the absorber.Two sets of data on the boundary were sampled,respectively,before and after the absorber was embedded inside the tissue-like medium.The two sets of data were normalized and then used to reconstruct the absorption coefficient distribution.The recovered image reflected the real location and size of the absorber.Conclusion The proposed imaging platform can image the tissue optical parameters effectively.However the resolution of the reconstructed image was not high because the inverse problem was gravely underdetermined and the noise was not considered in the reconstruction algorithm.The recovered result in the next generation system could be improved by making more use of prior information and enhancing the performance of the system.

AIM: To establish chromatography fingerprint of Radix Ophiopogonis. METHODS: HPLC was applied on a Kromasil C_(18) column(5 ?m,4.6 mm?250 mm) with CH_3CN-0.01%H_3PO_4 solution by gradient elution,flow-rate of 1.0 mL/min,and the UV absorbance was monitored at 297 nm. RESULTS: 30 common peaks were picked up. CONCLUSION: This method is reliable,simple and provides a reference standard for the quality control of Radix Ophiopogonis.